scholarly journals DEVELOPMENTAL MODIFICATIONS IN THE SAND DOLLAR CAUSED BY COBALTOUS CHLORIDE IN COMBINATION WITH SODIUM SELENITE AND ZINC CHLORIDE

1957 ◽  
Vol 113 (3) ◽  
pp. 480-487 ◽  
Author(s):  
OLIN RULON
Keyword(s):  
Zinc Chloride ◽  
Sodium Selenite ◽  
Sand Dollar ◽  
Cobaltous Chloride

Effect of zinc chloride and sodium selenite supplementation on in vitro maturation, oxidative biomarkers, and gene expression in buffalo (Bubalus bubalis) oocytes

Zygote ◽  
2021 ◽  
pp. 1-8
Author(s):  
Wael A. Khalil ◽  
Chun-Yan Yang ◽  
Mostafa M. El-Moghazy ◽  
Mohamed S. El-Rais ◽  
Jiang-Hua Shang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Zinc Chloride ◽  
Sodium Selenite ◽  
Expression Patterns ◽  
Basic Medium ◽  
Expression Levels ◽  
Maturation Medium ◽  
Oxidative Biomarkers

Summary This study examined the effects of zinc chloride (ZnCl2) and sodium selenite (Na2SeO3) supplementation in maturation medium on in vitro maturation (IVM) rate, oxidative biomarkers and gene expression in buffalo oocytes. Ovaries from a slaughterhouse were aspirated and good quality cumulus–oocyte complexes (COCs) with at least four layers of compact cumulus cells and evenly granulated dark ooplasm were selected. COCs were randomly allocated during IVM (22 h) to one of four treatment groups: (1) control maturation medium (basic medium), or basic medium supplemented with (2) ZnCl2 (1.5 µg/ml), (3) Na2SeO3 (5 µg/l), or (4) ZnCl2 + Na2SeO3 (1.5 µg/ml + 5 µg/l, respectively). Oocytes were denuded after 22 h of IVM in the first four replicates. Specimens were fixed and stained to evaluate the stage of nuclear maturation. The spent medium was collected for biochemical assays of total antioxidant capacity (TAC), malondialdehyde (MDA) and hydrogen peroxide concentrations. A second four replicates were used for COCs for RNA extraction. The expression levels of antioxidant (SOD1, GPX4, CAT and PRDX1), antiapoptotic (BCL2 and BCL-XL) and proapoptotic (BAX and BID) genes were measured. Supplementation with ZnCl2 and Na2SeO3 during IVM increased the ratio of oocytes reaching metaphase II at 22 h, increased TAC and decreased MDA and H2O2 concentrations in the maturation medium (P < 0.05). Moreover, beneficial effects were associated with complementary changes in expression patterns of antioxidative, antiapoptotic and proapoptotic genes, suggesting lower oxidative stress and apoptosis. Supplementation medium with zinc chloride and sodium selenite improves the maturation rate, reduces oxidative stress and increases expression levels of antioxidative and antiapoptotic genes.

EFFECTS OF COBALTOUS CHLORIDE

1969 ◽  
Vol 61 (2_Suppl) ◽  
pp. S128-S149
Author(s):  
Sture Falkmer
Keyword(s):  
Cobaltous Chloride

Impact of Sodium Selenite on Antioxidative Metabolism in Kidney Tissue of Albino Rat Under Ammonia Stress

2012 ◽  
Vol 3 (1) ◽  
pp. 489-490
Author(s):  
M. SIVA SANKAR M. SIVA SANKAR ◽  
◽  
K. SUJATHA K. SUJATHA ◽  
P. NEERAJA P. NEERAJA
Keyword(s):  
Sodium Selenite ◽  
Kidney Tissue ◽  
Albino Rat ◽  
Antioxidative Metabolism ◽  
Ammonia Stress
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