Comparative Effects of Cobaltous Chloride, Potassium Ferricyanide, and Zinc Chloride on Reconstitution in Planarians

1955 ◽  
Vol 28 (4) ◽  
pp. 330-339
Author(s):  
Rosemary R. Hein
Keyword(s):  
Zinc Chloride ◽  
Potassium Ferricyanide ◽  
Cobaltous Chloride

TEM comparison of osmium vs osmium with potassium ferricyanide secondary fixatives and the impact of secondary fixative temperature on tissue preservation/contrast quality

1996 ◽  
Vol 54 ◽  
pp. 910-911
Author(s):  
J. W. Horn ◽  
B. J. Dovey-Hartman ◽  
V. P. Meador
Keyword(s):  
Osmium Tetroxide ◽  
Potassium Ferricyanide ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Microscopic Evaluation ◽  
Cacodylate Buffer ◽  
Transmission Electron Microscopic ◽  
Glutaraldehyde Solution ◽  
Temperature Impact ◽  
The Impact

Osmium tetroxide (OsO4) is a universally used secondary fixative for routine transmission electron microscopic evaluation of biological specimens. Use of OsO4 results in good ultrastructural preservation and electron density but several factors, such as concentration, length of exposure, and temperature, impact overall results. Potassium ferricyanide, an additive used primarily in combination with OsO4, has mainly been used to enhance the contrast of lipids, glycogen, cell membranes, and membranous organelles. The purpose of this project was to compare the secondary fixative solutions, OsO4 vs. OsO4 with potassium ferricyanide, and secondary fixative temperature for determining which combination gives optimal ultrastructural fixation and enhanced organelle staining/contrast.Fresh rat liver samples were diced to ∼1 mm3 blocks, placed into porous processing capsules/baskets, preserved in buffered 2% formaldehyde/2.5% glutaraldehyde solution, and rinsed with 0.12 M cacodylate buffer (pH 7.2). Tissue processing capsules were separated (3 capsules/secondary fixative.solution) and secondarily fixed (table) for 90 minutes. Tissues were buffer rinsed, dehydrated with ascending concentrations of ethanol solutions, infiltrated, and embedded in epoxy resin.

EFFECTS OF COBALTOUS CHLORIDE

1969 ◽  
Vol 61 (2_Suppl) ◽  
pp. S128-S149
Author(s):  
Sture Falkmer
Keyword(s):  
Cobaltous Chloride

Investigation of characteristics of pulse discharge about surface water solution of zinc chloride

2016 ◽  
Vol 39 (0) ◽  
pp. 100-105
Author(s):  
В. В. Данило ◽  
І. В. Шевера ◽  
Й. О. Миня ◽  
З. Т. Гомокі ◽  
Д. А. Калімуліна ◽  
...  
Keyword(s):  
Surface Water ◽  
Zinc Chloride ◽  
Water Solution ◽  
Pulse Discharge

Use of Low Pressure Mercury Lamps for the Photodecomposition of Iron Cyanide in Gold Mill Effluents

1985 ◽  
Vol 20 (2) ◽  
pp. 111-119 ◽  
Author(s):  
J.H. Carey ◽  
S.A. Zaidi
Keyword(s):  
Ultraviolet Light ◽  
High Intensity ◽  
Experimental Evaluation ◽  
Low Pressure ◽  
Pilot Scale ◽  
Weak Acid ◽  
Potassium Ferricyanide ◽  
Technical Feasibility ◽  
Iron Cyanide ◽  
Scale Test

Abstract The use of ultraviolet light (UV) from low pressure mercury lamps for destroying iron cyanide in synthetic and actual gold mill effluents was evaluated in this study. For the light intensities used in this study, UV irradiation was not able to efficiently destroy cyanide. However, it converted iron cyanide to a weak acid dissociable form which was destroyed by chlorine. Data from several bench-scale tests and one pilot scale test were used to estimate quantum efficiencies (moles iron cyanide destroyed/einstein). These efficiencies ranged from 0.2% to 1%; approximately 30% to 90% lower than those reported in the literature for potassium ferricyanide. The data collected during the study demonstrated the technical feasibility of using UV in conjunction with chlorination for destroying iron cyanide in gold mill effluents. However, low pressure mercury lamps do not appear to be a practical UV source for this purpose. Irradiation with high intensity lamps may be more practical and is recommended for experimental evaluation.

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