Functions of Bacterial Cell Surface Structures

BioScience ◽  
1985 ◽  
Vol 35 (3) ◽  
pp. 172-177 ◽  
Author(s):  
F. G. Ferris ◽  
T. J. Beveridge
Keyword(s):  
Cell Surface ◽  
Bacterial Cell ◽  
Surface Structures ◽  
Bacterial Cell Surface ◽  
Cell Surface Structures

scholarly journals Phosphoethanolamine cellulose enhances curli-mediated adhesion of uropathogenicEscherichia colito bladder epithelial cells

2018 ◽  
Vol 115 (40) ◽  
pp. 10106-10111 ◽  
Author(s):  
Emily C. Hollenbeck ◽  
Alexandra Antonoplis ◽  
Chew Chai ◽  
Wiriya Thongsomboon ◽  
Gerald G. Fuller ◽  
...  
Keyword(s):  
Cell Surface ◽  
Bacterial Cell ◽  
Cell Monolayer ◽  
Surface Structures ◽  
Bacterial Cell Surface ◽  
Cell Surface Structures ◽  
Bladder Cell ◽  
Type 1 Pili ◽  
Tract Infections

UropathogenicEscherichia coli(UPEC) are the major causative agents of urinary tract infections, employing numerous molecular strategies to contribute to adhesion, colonization, and persistence in the bladder niche. Identifying strategies to prevent adhesion and colonization is a promising approach to inhibit bacterial pathogenesis and to help preserve the efficacy of available antibiotics. This approach requires an improved understanding of the molecular determinants of adhesion to the bladder urothelium. We designed experiments using a custom-built live cell monolayer rheometer (LCMR) to quantitatively measure individual and combined contributions of bacterial cell surface structures [type 1 pili, curli, and phosphoethanolamine (pEtN) cellulose] to bladder cell adhesion. Using the UPEC strain UTI89, isogenic mutants, and controlled conditions for the differential production of cell surface structures, we discovered that curli can promote stronger adhesive interactions with bladder cells than type 1 pili. Moreover, the coproduction of curli and pEtN cellulose enhanced adhesion. The LCMR enables the evaluation of adhesion under high-shear conditions to reveal this role for pEtN cellulose which escaped detection using conventional tissue culture adhesion assays. Together with complementary biochemical experiments, the results support a model wherein cellulose serves a mortar-like function to promote curli association with and around the bacterial cell surface, resulting in increased bacterial adhesion strength at the bladder cell surface.

Bacterial Cell Surface Structures in Yersinia enterocolitica

2012 ◽  
Vol 60 (3) ◽  
pp. 199-209 ◽  
Author(s):  
Nataniel Białas ◽  
Katarzyna Kasperkiewicz ◽  
Joanna Radziejewska-Lebrecht ◽  
Mikael Skurnik
Keyword(s):  
Cell Surface ◽  
Yersinia Enterocolitica ◽  
Bacterial Cell ◽  
Surface Structures ◽  
Bacterial Cell Surface ◽  
Cell Surface Structures

Micro-organism and Cell Viability on Antimicrobially Modified Titanium

2009 ◽  
Vol 88 (10) ◽  
pp. 957-962 ◽  
Author(s):  
S. Omori ◽  
Y. Shibata ◽  
T. Arimoto ◽  
T. Igarashi ◽  
K. Baba ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Cell Surface ◽  
Streptococcus Mutans ◽  
Bacterial Cell ◽  
Serum Proteins ◽  
Surface Structures ◽  
Osteoblastic Cells ◽  
Nacl Solution ◽  
Cell Surface Structures ◽  
Micro Organisms

When titanium is anodized by discharge in NaCl solution, both antimicrobial activity and osteoconductivity are conferred. The viability of adherent micro-organisms and cells on antimicrobial titanium remains uncertain. We hypothesized that a thin peroxidation barrier would efficiently destroy adherent bacteria, whereas adherent osteoblastic cells would be viable, since these cells adhere to the surface indirectly though serum proteins. The efficacy of antimicrobial titanium appears to be based on peroxidation, since peroxidation products were detected in parallel with the destruction of bacterial cell-surface structures. The peroxidation effect of antimicrobial titanium was confined to the surface within narrow limits. The viability of osteoblastic cells on the surface was strongly dependent on the presence of serum protein, whereas that of adherent Streptococcus mutans was not affected by the presence of serum proteins. Therefore, differences in the adherent systems used by bacteria and osteoblastic cells are important determinants of their viability on antimicrobial titanium.

Fe(iii)-complex mediated bacterial cell surface immobilization of eGFP and enzymes

2021 ◽  
Author(s):  
Lilin Feng ◽  
Liang Gao ◽  
Daniel F. Sauer ◽  
Yu Ji ◽  
Haiyang Cui ◽  
...  
Keyword(s):  
Cell Surface ◽  
Bacterial Cell ◽  
Surface Immobilization ◽  
E Coli ◽  
Bacterial Cell Surface ◽  
Reversible Method

A facile and reversible method to immobilize His6-tagged proteins on the E. coli cell surface through the formation of an Fe(iii)-complex.

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