scholarly journals Smoking, alcohol consumption and human papillomavirus infection as risk factors for oral cavity and oropharyngeal tumors in Serbia - a pilot study

2020 ◽  
Vol 77 (7) ◽  
pp. 740-745
Author(s):  
Ljiljana Bozic ◽  
Predrag Jeremic ◽  
Milovan Dimitrijevic ◽  
Tanja Jovanovic ◽  
Aleksandra Knezevic
Keyword(s):  
Risk Factors ◽  
Human Papillomavirus ◽  
High Risk ◽  
Alcohol Consumption ◽  
Oral Cavity ◽  
Tongue Cancer ◽  
Hpv Infection ◽  
Benign Lesions ◽  
Significant Difference ◽  
Malignant Lesions

Background/Aim. The oral cavity and oropharyngeal cancers are among the most common cancers worldwide with the multifactorial etiology. The aim of this study was to determine the major risk factors among patients with oral cavity and oropharyngeal tumors in Serbia. Methods. A total of 63 patients with biopsy proven malignant (33 patients) or benign (30 patients) oral cavity or oropharyngeal lesions were included in this study. The data about gender, age, smoking habits and alcohol consumption were obtained from the routine medical files. The detection and genotyping of human papillomavirus (HPV) was done in paraffin embedded tissue samples using in situ hybridization. Results. Malignant lesions were more frequent in men, smokers and patients who consume alcohol with a statistically significant difference compared to the patients with benign lesions. The prevalence of HPV infection was higher in patients with malignant lesions compared to patients with benign lesions, but without statistically significant difference. High risk genotypes were detected only in patients with malignant lesions of tonsils and base tongue cancer, while low risk types were demonstrated in patients with benign lesions with a highly statistically significant difference. Conclusion. The results point to the significant association of tobacco smoking, alcohol consumption and high risk HPV genotypes as risk factors for oral cavity and oropharyngeal carcinomas in Serbian patients.

scholarly journals Human papillomavirus infection rate, distribution characteristics, and risk of age in pre- and postmenopausal women

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Yan Shen ◽  
Jing Xia ◽  
Huihui Li ◽  
Yang Xu ◽  
Sanping Xu
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
High Risk ◽  
Postmenopausal Women ◽  
Infection Rate ◽  
Hpv Infection ◽  
The Other ◽  
Distribution Characteristics ◽  
Significant Difference ◽  
High Risk Hpv

Abstract Background The incidence rate of cervical cancer is increasing yearly. The persistent infection of high-risk human papillomavirus (HPV) is the main factor leading to cervical cancer. HPV infection is double peak type. This study aimed at analyzing the HPV distribution characteristics, infection rate, and risk of age in pre- and postmenopausal women. So as to provide reference for the prevention of HPV infection and cervical cancer screening strategy. Methods A retrospective analysis of 4614 women who underwent cervical cytology, and HPV examination from January 2018 to October 2019 at the healthcare department of Wuhan Union Hospital was done. We explored the characteristics and distribution of HPV infections around the menopause, then comparing the infection rate of HPV in postmenopause and over 65 years old, in order to analyze the influence of different ages on HPV infection. Results Generally, the HPV infection rate was 13.10% (539/4115), whereby the high-risk subtype constituted 73.84% (398/539) of all positive cases. On the other hand, the HPV39 infection was more common in postmenopausal women; however, there was no significant difference in the distribution of the other types in the pre- and postmenopausal women. The first four types were 52/53/58/16. The results further showed that the rates of HPV infection before and after menopause were 12.34% (367/2975) and 15.09% (172/1140), respectively, which had no significant difference (P = 0.056), but more susceptible to high-risk HPV infection after the age of 65 (P = 0.041). Except for 40 years old to menopause, the infection rate of high-risk HPV in this age group was different from that in postmenopause (P = 0.023, 0.729 (0.555, 0.957)), other age groups had no significant effect on high-risk HPV infection. Conclusions It was concluded that whether menopause has nothing to do with HPV infection. Moreover, the risk of high-risk HPV infection in women aged 40 to premenopausal is relatively low, but the infection rate increases after 65. Hence the cutoff screening age should be appropriately prolonged.

scholarly journals Portrait of DNA methylated genes predictive of poor prognosis in head and neck cancer and the implication for targeted therapy

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jessica Hier ◽  
Olivia Vachon ◽  
Allison Bernstein ◽  
Iman Ibrahim ◽  
Alex Mlynarek ◽  
...  
Keyword(s):  
Risk Factors ◽  
Head And Neck Cancer ◽  
High Risk ◽  
Head And Neck ◽  
Neck Cancer ◽  
Tongue Cancer ◽  
Hpv Infection ◽  
Environmental Carcinogens ◽  
Dna Hypermethylation ◽  
Regulatory Pathways

AbstractIn addition to chronic infection with human papilloma virus (HPV) and exposure to environmental carcinogens, genetic and epigenetic factors act as major risk factors for head and neck cancer (HNC) development and progression. Here, we conducted a systematic review in order to assess whether DNA hypermethylated genes are predictive of high risk of developing HNC and/or impact on survival and outcomes in non-HPV/non-tobacco/non-alcohol associated HNC. We identified 85 studies covering 32,187 subjects where the relationship between DNA methylation, risk factors and survival outcomes were addressed. Changes in DNA hypermethylation were identified for 120 genes. Interactome analysis revealed enrichment in complex regulatory pathways that coordinate cell cycle progression (CCNA1, SFN, ATM, GADD45A, CDK2NA, TP53, RB1 and RASSF1). However, not all these genes showed significant statistical association with alcohol consumption, tobacco and/or HPV infection in the multivariate analysis. Genes with the most robust HNC risk association included TIMP3, DCC, DAPK, CDH1, CCNA1, MGMT, P16, MINT31, CD44, RARβ. From these candidates, we further validated CD44 at translational level in an independent cohort of 100 patients with tongue cancer followed-up beyond 10 years. CD44 expression was associated with high-risk of tumor recurrence and metastasis (P = 0.01) in HPV-cases. In summary, genes regulated by methylation play a modulatory function in HNC susceptibility and it represent a critical therapeutic target to manage patients with advanced disease.

scholarly journals Human Papillomavirus and Infertility

Medicina ◽  
2019 ◽  
Vol 55 (7) ◽  
pp. 377 ◽  
Author(s):  
Jeršovienė ◽  
Gudlevičienė ◽  
Rimienė ◽  
Butkauskas
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Sperm Quality ◽  
Hpv Infection ◽  
University Hospital ◽  
Sperm Parameters ◽  
World Health ◽  
Abnormal Sperm ◽  
Significant Difference ◽  
High Risk Hpv

Background and objectives. Human papillomavirus (HPV) is the most commonly sexually transmitted infection. Recent evidence suggests that an HPV infection may affect fertility. The aim of the study was to determine the prevalence of HPV infections among couples undergoing in vitro fertilization (IVF) and to identify their awareness of HPV. Material and Methods. A total of 200 samples were collected from couples who received IVF treatment during 2017–2018 in Vilnius University Hospital Santaros Klinikos (VUH SK) Santaros Fertility Centre (SFC). For HPV detection, cervical swabs from women and sperm samples from men were taken and a real time polymerase chain reaction (RT-PCR) was used for the identification of 14 high-risk HPV types. Sperm parameters were evaluated according to World Health Organization (WHO) recommendations for 2010. Research subjects answered an anonymous questionnaire to ascertain their knowledge of HPV. Results. After testing of HPV in couples undergoing IVF, it was found that 33 out of 100 couples (33%) were HPV positive. Of these, 19% of women (19/100) and 20% of men (20/100) tested positive. Using Fisher’s exact test, a statistically significant difference was found between HPV infections and abnormal sperm quality parameters (p = 0.023). Conclusions. HPV may have an impact in spermatogenesis, because an HPV infection was more frequently detected in men with abnormal sperm parameters. High-risk HPV 52 was the most common genotype among couples undergoing IVF treatment.

Prevalence of Human Papillomavirus in Malignant Lesions of the Oral Cavity and its Association With Risk Factors for Oral Cancer

2015 ◽  
Vol 120 (2) ◽  
pp. e102
Author(s):  
KAMILE LEONARDI DUTRA ◽  
BRUNO EDUARDO SILVA DE ARAUJO ◽  
MARIA LUIZA BAZZO ◽  
ELENA RIET CORREA RIVERO ◽  
ALEXANDRE SHERLLEY CASIMIRO ONOFRE
Keyword(s):  
Risk Factors ◽  
Human Papillomavirus ◽  
Oral Cancer ◽  
Oral Cavity ◽  
Malignant Lesions

Alcohol consumption and persistent infection of high-risk human papillomavirus

2014 ◽  
Vol 143 (7) ◽  
pp. 1442-1450 ◽  
Author(s):  
H. Y. OH ◽  
M. K. KIM ◽  
S. SEO ◽  
D. O. LEE ◽  
Y. K. CHUNG ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Alcohol Consumption ◽  
Hpv Infection ◽  
Cancer Center ◽  
Cervical Carcinogenesis ◽  
Multivariate Logistic Regression ◽  
Major Step ◽  
High Consumption ◽  
Drinking Frequency

SUMMARYAlcohol consumption is a possible co-factor of high-risk human papillomavirus (HR-HPV) persistence, a major step in cervical carcinogenesis, but the association between alcohol and continuous HPV infection remains unclear. This prospective study identified the association between alcohol consumption and HR-HPV persistence. Overall, 9230 women who underwent screening during 2002–2011 at the National Cancer Center, Korea were analysed in multivariate logistic regression. Current drinkers [odds ratio (OR) 2·49, 95% confidence interval (CI) 1·32–4·71] and drinkers for ⩾5 years (OR 2·33, 95% CI 1·17–4·63) had a higher risk of 2-year HR-HPV persistence (HPV positivity for 3 consecutive years) than non-drinkers and drinkers for <5 years, respectively (vs. HPV negativity for 3 consecutive years). A high drinking frequency (⩾twice/week) and a high beer intake (⩾3 glasses/occasion) had higher risks of 1-year (OR 1·80, 95% CI 1·01–3·36) HPV positivity for 2 consecutive years) and 2-year HR-HPV persistence (OR 3·62, 95% CI 1·35–9·75) than non-drinkers. Of the HPV-positive subjects enrolled, drinking habit (OR 2·68, 95% CI 1·10–6·51) and high consumption of beer or soju (⩾2 glasses/occasion; OR 2·90, 95% CI 1·06–7·98) increased the risk of 2-year consecutive or alternate HR-HPV positivity (vs. consecutive HPV negativity). These findings suggest that alcohol consumption might increase the risk of cervical HR-HPV persistence in Korean women.

scholarly journals Necessity for subsequent surgery in women of child-bearing age with positive margins after conization

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Xinmei Wang ◽  
Juan Xu ◽  
Yang Gao ◽  
Pengpeng Qu
Keyword(s):  
Risk Factors ◽  
Risk Factor ◽  
High Risk ◽  
Infection Type ◽  
Hpv Infection ◽  
Transformation Zone ◽  
Positive Margins ◽  
Child Bearing ◽  
Significant Difference ◽  
Residual Lesions

Abstract Background 20–25% of women with high-grade cervical intraepithelial neoplasias (HSIL) have residual lesions after conization. The state of the margin is generally considered to be a risk factor for recurrence or persistent lesions. Predictors of positive margins and residual lesions need to be identified. A design for postoperative management and avoidance of overtreatment needs to be provided, especially for women of child-bearing age. Methods This study was a retrospective analysis of 1309 women of child-bearing age with HSIL, who underwent cold knife conization (CKC). Age, gravidity, parity, human papillomavirus (HPV) species, cytology, transformation zone type, results of endocervical curettage (ECC), quadrant involvement, glandular involvement, and Cervical Intraepithelial Neoplasia (CIN) grade were analyzed. Among those with positive margins, 245 patients underwent secondary surgery within three months, including CKC, a loop electrosurgical excision procedure, and total hysterectomy. Risk factors for positive margins and residual lesions were assessed. Results There was no significant difference in age, gravidity, parity, glandular involvement, and CIN grade between the two groups (P > 0.3). There was a significant difference in HPV species, cytology, ECC results, and quadrant involvement (P < 0.002). Multivariate analysis showed a major cytology abnormality, high-risk HPV infection, type III transformation zone, positive ECC result, and multiple quadrant involvement were independent risk factors for positive margins and residual lesions (P < 0.02). Age > 35 years was also a risk factor for residual lesions (P < 0.03). Conclusion High-risk women should be treated appropriately considering fertility. Patients with positive margins should be managed uniquely. Surgery for women without fertility may be appropriate. Close follow-up is necessary for women who have fertility requirements or are unwilling to undergo subsequent surgery if they have no risk factors, especially women < 35 years.

Risk Factors for High-Risk Human Papillomavirus Infection and Cofactors for High-Grade Cervical Disease in Peru

2011 ◽  
Vol 21 (9) ◽  
pp. 1654-1663 ◽  
Author(s):  
Maribel Almonte ◽  
Catterina Ferreccio ◽  
Miguel Gonzales ◽  
Jose Manuel Delgado ◽  
C. Hilary Buckley ◽  
...  
Keyword(s):  
Risk Factors ◽  
Human Papillomavirus ◽  
High Risk ◽  
Hpv Infection ◽  
High Grade ◽  
Papanicolaou Test ◽  
First Sexual Intercourse ◽  
Papillomavirus Infection ◽  
Cervical Disease ◽  
Potential Risk Factors

ObjectiveTo evaluate the association between potential risk factors for high-risk human papillomavirus (HR-HPV) infection and cofactors for cervical intraepithelial lesions grade 2 or worse (CIN2+) in women attending cervical screening in Amazonian Peru.Materials and MethodsParticipants completed a risk factor questionnaire before screening. High-risk human papillomavirus infection was determined by Hybrid Capture II. Logistic regression was used to evaluate associations between potential risk factors for HR-HPV infection and between cofactors and risk of CIN2+ among women with HR-HPV infection.ResultsScreening and questionnaires were completed by 5435 women aged 25 to 49 years. The prevalence of HR-HPV was 12.6% (95% confidence interval [CI], 11.8%–13.6%) and decreased by age. Early age at first sexual intercourse and several lifetime sexual partners increased the risk of having HR-HPV (age-adjusted odds ratio [AOR] of age at first sexual intercourse <18 vs ≥20, 1.5; 95% CI, 1.2–2.0; AOR of ≥5 lifetime sexual partners vs 1, 2.1; 95% CI, 1.4–3.2). Among women with HR-HPV infection, those with no schooling (AOR relative to 1–5 years of schooling, 3.2; 95% CI, 1.3–8.3) and those with parity ≥3 (AOR relative to parity <3, 2.6; 95% CI, 1.4–4.9) were at increased risk of CIN2+. The effect of parity was stronger for cancer (AOR of parity ≥3 vs <3, 8.3; 95% CI, 1.0–65.6). Further analysis showed that the association between parity and CIN2+ was restricted to women younger than 40. Most women (83%) had previously been screened. Sixty-four percent of CIN2+ cases detected in this study occurred in women who reported having had a Papanicolaou test in the previous 3 years. Only 4 of 20 cancers were detected in women never screened before. Having had a previous abnormal Papanicolaou test increased the risk of CIN2+ (OR, 16.1; 95% CI, 6.2–41.9).ConclusionAmong women with HR-HPV, high parity (in young women), no schooling, lack of good-quality screening and of adequate follow-up care are the main risk factors for high-grade cervical disease in Peru.

Correlation between the diversity of vaginal microbiota and the risk of high-risk human papillomavirus infection

2019 ◽  
Vol 29 (1) ◽  
pp. 28-34 ◽  
Author(s):  
Xiao-Pei Chao ◽  
Ting-Ting Sun ◽  
Shu Wang ◽  
Qing-Bo Fan ◽  
Hong-Hui Shi ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Statistical Difference ◽  
Hpv Infection ◽  
Cervical Neoplasia ◽  
Vaginal Microbiota ◽  
Future Research ◽  
Hexadecyltrimethylammonium Bromide ◽  
Significant Difference ◽  
High Risk Hpv

ObjectivesSince other genital infections enhance HIV susceptibility by inducing inflammation and evidence suggests that the vaginal microbiome plays a functional role in the persistence or regression of high-risk human papillomavirus (HPV) infections, we investigated the relationship between the composition of the vaginal microbiota and the risk of high-risk HPV infection.MethodsThe study included 151 healthy women (65 HPV-positive and 86 HPV-negative) aged 20–65 at enrollment. Total genome DNA from samples was extracted using the hexadecyltrimethylammonium bromide (CTAB) CTAB method. The vaginal microbiota composition was determined by sequencing barcoded 16S rDNA gene fragments (V4) on Illumina HiSeq2500.ResultsOf the 30 most abundant bacteria at the genus level, we found only six bacteria with a statistical difference between HPV-positive and HPV-negative women: Bacteroides, Acinetobacter, Faecalibacterium, Streptococcus, Finegoldia, and Moryella. Lactobacillus was the predominant genus and was detected in all women, but there was no significant difference between the two groups for L. iners, L. jensenii, and L. gasseri. Furthermore, we found 26 types of bacteria with a statistical difference at the species level between the two groups. Anaerobic bacteria such as Bacteroides plebeius, Acinetobacter lwoffii, and Prevotella buccae were found significantly more frequently in HPV-positive women, which is the most important finding of our study.ConclusionOur findings suggest a possible role for the composition of the vaginal microbiota as a modifier of high-risk HPV infection, and specific microbiota species may serve as sensors for changes in the cervical microenvironment associated with high-risk HPV infection. The exact molecular mechanism of the vaginal microbiota in the course of high-risk HPV infection and cervical neoplasia should be further explored. Future research should include intervention in the composition of the vaginal microbiota to reverse the course of high-risk HPV infection and the natural history of cervical neoplasia.

scholarly journals The Correlation Between Bacterial Vaginosis, High-Risk Human Papillomavirus Infection, and Cervical Intraepithelial Neoplasia Progression

2021 ◽  
Author(s):  
Xiaolin Xu ◽  
Yichan Zhang ◽  
Liqun Yu ◽  
Xingxian Shi ◽  
Min Min ◽  
...  
Keyword(s):  
Risk Factors ◽  
Human Papillomavirus ◽  
High Risk ◽  
Bacterial Vaginosis ◽  
Cervical Intraepithelial Neoplasia ◽  
Protective Factor ◽  
Intraepithelial Neoplasia ◽  
Hpv Infection ◽  
Vaginal Microbiota ◽  
Papillomavirus Infection

Abstract Persistent infection with high-risk human papillomavirus (HR-HPV) is an important reason for the progression of cervical intraepithelial neoplasia (CIN). Bacterial vaginosis (BV) is a genital infection that frequently presents in women infected with HPV, but the correlation between BV and HPV during CIN development is still elusive. In this study, we enrolled 624 participants and obtained 423 samples of vaginal secretions from them, including 193 HPV-negative samples and 230 HPV-positive samples. We used 16S rRNA sequencing to measure the vaginal microbiota diversity in women with or without BV and HPV co-infection and then calculated risk factors for CIN progression by logistic regression. We found that condom use (OR=3.480; 95% CI=1.069-11.325; P < .05) was a protective factor against CIN, whereas BV (OR=0.358; 95% CI=0.195-0.656; P < .05) and HR-HPV infection (OR= 0.016; 95% CI=0.004-0.072; P < .001) were risk factors for CIN. BV and HPV infection could trigger an increase in the diversity of vaginal microbiota and decrease Lactobacillus domination, which is conducive to CIN regression. The depletion of the carbohydrate metabolism pathway may induce Lactobacillus reduction. Treating BV in the clinical setting could block CIN development and L. iners may be a crucial species during this process.

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