scholarly journals Morphometric analysis of the fascicular organisation of the optic nerve

2015 ◽  
Vol 72 (2) ◽  
pp. 132-135
Author(s):  
Miroslav Radunovic ◽  
Zdravko Vitosevic ◽  
Mila Cetkovic ◽  
Aleksandra Vuksanovic-Bozaric ◽  
Nemanja Radojevic ◽  
...  
Keyword(s):  
Optic Nerve ◽  
Connective Tissue ◽  
Blood Vessels ◽  
Morphometric Analysis ◽  
Testing System ◽  
Entire Surface ◽  
Optic Nerves ◽  
Different Parts

Background/Aim. The optic nerve is anatomically observed in four segments: intrabulbar, orbital, canalicular, and cranial. According to the literature, the surface of the transversal cut of the nerve is different through it. The aim of this study was to evaluate the fascicular organisation of the optic nerve, throughout its three segments from the eye. Methods. Five pairs of optic nerves, obtained from the autopsies were examined. Using Heidenhain's (azan) staining, the cuts were prepared for microscopy. Morphometric analysis was performed using the stereological methods for morphometric cytology - the Weible?s testing system M42. The following measures were established: the surface of the transverse cut of the nerve, the entire surface of fasciculi, the entire surface of connective tissue and blood vessels, the number of fasciculi, the surface of a single fasciculus. Results. The surface of the transverse cut of the nerve was found to grow from the orbital to the cranial segment, as well as the entire surface of fasciculi. While their number is significantly lower in the cranial segment, the number of fasciculi varied slightly between the orbital and the canalicular segment. The surface of a single fasciculus grows from the bulb to the chiasma. There is probable a cause to believe that this may be due to fusion of the ?small? fasciculi in the orbitocranial direction. Conclusion. There are significant differences among the examined parameters of the different parts of the optic nerve.

Permeability of blood vessels and connective tissue sheaths in retina and optic nerve

1973 ◽  
Vol 26 (2) ◽  
pp. 147-156 ◽  
Author(s):  
Yngve Olsson ◽  
Krister Kristensson
Keyword(s):  
Optic Nerve ◽  
Connective Tissue ◽  
Blood Vessels

Regeneration in the rat optic nerve after cold injury

1989 ◽  
Vol 71 (2) ◽  
pp. 254-265 ◽  
Author(s):  
Mineko Murakami ◽  
Chizuka Ide ◽  
Haruyuki Kanaya
Keyword(s):  
Optic Nerve ◽  
Connective Tissue ◽  
Blood Vessels ◽  
Smooth Endoplasmic Reticulum ◽  
Light And Electron Microscopy ◽  
Central Zone ◽  
Content Type ◽  
Adult Rat ◽  
Astrocyte Endfeet ◽  
Fine Print

✓ In order to examine nerve regeneration under conditions in which the basal laminae of the glial limiting membranes (GLM) and blood vessels were preserved intact, the intraorbital segment of adult rat optic nerve was frozen locally. During the next 3 months, degenerative and regenerative changes in axons and glial cells were observed by light and electron microscopy. On the day after treatment, all the myelinated and unmyelinated axons in the central zone of the lesion were damaged. The astrocyte endfeet of the GLM as well as the blood vessels were extensively disrupted, while their basal laminae were preserved apparently intact as a continuous sheet. Three days after treatment, regenerating axons appeared in the central zone of the lesion. They contained various numbers of clear and dense-cored vesicles as well as some smooth endoplasmic reticulum. The regenerating axons gradually increased in number, especially beneath the pial and perivascular surfaces of the lesion, where an abundance of regenerating axons was found 3 months after treatment. A few of these axons were abnormally remyelinated by oligodendrocytes. In addition to this axonal regeneration through the intraoptic nerve compartment, fine regenerating axons were seen growing out through GLM into the pial connective tissue 3 weeks after treatment. Astrocyte endfeet of the GLM became irregular in contour, protruding in a fern-leaf fashion into the pial connective tissue. Fine naked axons grew out through these protrusions and subsequently increased in number, vigorously growing in large bundles both proximally and distally along blood vessels in the pial connective tissue. Bundles of regenerating axons extended as much as 1.5 mm from the site of the lesion 3 months after surgery. These bundles were covered by thin processes of pial or arachnoidal non-neuronal cells, and the regenerating axons remained unmyelinated. The above findings indicate that under well-nourished conditions, adult mammalian optic nerve exhibits considerable regenerative ability.

Characterization of a goldfish antigen during development and regeneration of the visual system

1989 ◽  
Vol 2 (5) ◽  
pp. 449-454 ◽  
Author(s):  
S. B. Braverman ◽  
I. Rappaport ◽  
S. C. Sharma
Keyword(s):  
Monoclonal Antibody ◽  
Optic Nerve ◽  
Connective Tissue ◽  
Visual System ◽  
Neuronal Cells ◽  
Müller Cells ◽  
Muller Cells ◽  
Optic Nerves

AbstractNormal, regenerating, and developing optic nerves of the goldfish were studied utilizing a monoclonal antibody (mAb) 1E1T which has specificity for Müller cells in the retina, radial ghal cells in the tectum, and non-neuronal cells in the optic nerve.Sections of the normal optic nerve revealed longitudinally oriented chains of non-neuronal cells, that were 4–8 cells long The number of chains in the normal nerve was very few. In addition, short acellular septa, probably the connective tissue septa, were also labeled with mAb 1E1T. Sections of crushed optic nerves showed an increase in the antigen recognized by mAb 1E1T within the septa and new septa were now visualized. Furthermore, the existing septa were longer and extended the length of the optic nerve. The formation and elongation of the septa occurred as early as 3 day postcrush. Between 3 and 11 d postcrush, there was heavy labeling of the septa and a large accumulation of non-neuronal cells at the crush site. At 3 months postcrush, the accumulation of non-neuronal cells labeled by mAb 1E1T were no longer visible but heavy labeling of the septa was still apparent.

scholarly journals Characteristics of perioptic and intraoptic arterial vascularization of extracranial segment of optic nerve

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Radmila Balaban-Djurević ◽  
Samra Hajrovic ◽  
Siniša Šolaja ◽  
Vanja Pljevčević ◽  
Valvita Reci ◽  
...  
Keyword(s):  
Nervous System ◽  
Optic Nerve ◽  
Blood Vessels ◽  
Ophthalmic Artery ◽  
Morphological Characteristics ◽  
Vascular Network ◽  
Branching Pattern ◽  
Optic Nerves ◽  
India Ink ◽  
Arterial Vascularization

Introduction. The optic nerve is supplied by the arterial branches of thesuperficial pial plexus and direct intraneural branches. The aim of the studywas to study the morphological characteristics (origin, number of arteries,calibers, and branching pattern) of perioptic arterial vascularization andintraoptic vascular network of the extracranial sections of the optic nerve:canalicular and intraorbital.Methods. The study included 18 pairs of the optic nerves of both sexes(11 males and 7 females), aged 51 to 78 years, with no signs of changes inthe structures of the nervous system. The vasculature of the optic nerveswas microdissected and examined under the stereoscopic microscope, afterinjecting their arteries with 10% mixture of India ink and gelatin, and5% formaline fixation.Results. The intracanalicular part of the optic nerve received fine bloodvessels from the ophthalmic artery, usually in two branches in 29 (82.9%)cases, while in 6 (17.1%) specimens there was only one branch for thenerve. The intraorbital segment of the optic nerve was supplied by twointerconnected systems of blood vessels, peripheral and axial, both comingfrom the central retinal artery.Conclusion. On the basis of the obtained results, it has been confirmedthat both segments of the optic nerve have a rich and very delicate vascularnetwork. The superficial pial arterial plexus of the intracanalicular segmentof the optic nerve has been identified as the only source of its vascularsupply. The intraorbital part of the optic nerve has two interconnectedsystems of blood vessels: peripheral and axial.

Early Detection of Central Nervous System Relapse of Pediatric Leukemia with Measurement of Optic Nerve Sheath Diameter on MRI

2019 ◽  
Vol 15 (2) ◽  
pp. 237-241
Author(s):  
Taner Arpaci ◽  
Barbaros S. Karagun
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Optic Nerve ◽  
Early Detection ◽  
Optic Nerve Sheath Diameter ◽  
Central Nervous System Relapse ◽  
Mri Findings ◽  
Pediatric Leukemia ◽  
Optic Nerves ◽  
Cns Relapse

Background: Leukemia is the most common pediatric malignancy. Central Nervous System (CNS) is the most frequently involved extramedullary location at diagnosis and at relapse. </P><P> Objective: To determine if Magnetic Resonance Imaging (MRI) findings of optic nerves should contribute to early detection of CNS relapse in pediatric leukemia. Methods: Twenty patients (10 boys, 10 girls; mean age 8,3 years, range 4-16 years) with proven CNS relapse of leukemia followed up between 2009 and 2017 in our institution were included. Orbital MRI exams performed before and during CNS relapse were reviewed retrospectively. Forty optic nerves with Optic Nerve Sheaths (ONS) and Optic Nerve Heads (ONH) were evaluated on fat-suppressed T2-weighted TSE axial MR images. ONS diameter was measured from the point 10 mm posterior to the globe. ONS distension and ONH configuration were graded as 0, 1 and 2. Results: Before CNS relapse, right mean ONS diameter was 4.52 mm and left was 4.61 mm which were 5.68 mm and 5.66 mm respectively during CNS relapse showing a mean increase of 25% on right and 22% on left. During CNS relapse, ONS showed grade 0 distension in 15%, grade 1 in 60%, grade 2 in 25% and ONH demonstrated grade 0 configuration in 70%, grade 1 in 25% and grade 2 in 5% of the patients. Conclusion: MRI findings of optic nerves may contribute to diagnose CNS relapse by demonstrating elevated intracranial pressure in children with leukemia.

scholarly journals Novel in vivo depiction of optic nerves hemorrhages in child abuse: a 3D-SWI pilot study

2021 ◽  
Author(s):  
Giulio Zuccoli
Keyword(s):  
Optic Nerve ◽  
Mass Effect ◽  
Abusive Head Trauma ◽  
Superficial Siderosis ◽  
Susceptibility Artifacts ◽  
Female Ratio ◽  
Optic Nerves ◽  
Statistical Correlations ◽  
Post Traumatic

Abstract Purpose Until now, the diagnosis of optic nerves hemorrhages in abusive head trauma (AHT) has been obtained only in the postmortem setting. The aim of the IRB-approved study was to assess the presence of optic nerves hemorrhages in AHT patients using 3D-SWI. Methods Thirteen children with a final confirmed multidisciplinary diagnosis of AHT underwent coronal and axial 3D-SWI imaging of the orbits. The presence of optic nerve sheath (ONS) hemorrhages was defined by thickening and marked 3D-SWI hypointensity of the ONS, resulting in mass effect upon the CSF space. Optic nerve (ON) hemorrhages were defined by areas of susceptibility artifacts in the ON parenchyma. Superficial siderosis was defined by susceptibility artifact coating the ON. Furthermore, data about post-traumatic deformity of the ONS at the head of the optic nerve were collected. Results The average age of the population was 7.9 ± 5.9 months old. The average GCS was 11.8 ± 4.5. The male to female ratio was 7:6. ONS hemorrhages were identified in 69.2% of cases. Superficial siderosis and ON hemorrhages were identified in 38.5 and 76.9% of cases, respectively. 3D-SWI also depicted traumatic deformity of the ONS at the level of the optic nerve head in 10 cases (76.9%). No statistical correlations were identified between RetCam findings and 3D-SWI findings or GCS and ON hemorrhages. Conclusion This research shows that dedicated MRI with volumetric SWI of the orbits can depict hemorrhages in the ON, ONS, and ONS injury, in AHT victims.

IV. Postscript to a paper “On the ganglia and nerves of the heart.”

1849 ◽  
Vol 139 ◽  
pp. 47-48
Keyword(s):  
Nervous System ◽  
Left Ventricle ◽  
Blood Vessels ◽  
Coronary Arteries ◽  
Royal Society ◽  
Posterior Surface ◽  
Anterior Surface ◽  
Entire Surface ◽  
Naked Eye ◽  
Nervous Structure

Since the communication above referred to was presented to the Royal Society, I have made a very minute dissection in alcohol of the whole nervous system of the young heifer’s heart. The distribution of the ganglia and nerves over the entire surface of the heart, and the relations of these structures to the blood-vessels and muscular substance, are far more fully displayed in these preparations than in any of my former dissections. On the anterior surface, there are distinctly visible to the naked eye ninety ganglia or ganglionic enlargements on the nerves, which pass obliquely across the arteries and the muscular fibres of the ventricles from their base to the apex. These ganglionic enlargements are observed on the nerves, not only where they are crossing the arteries, but where they are ramifying on the muscular substance without the blood-vessels. On the posterior surface, the principal branches of the coronary arteries plunge into the muscular substance of the heart near the base, and many nerves with ganglia accompany them throughout the walls to the lining membrane and columnse carneæ. From the sudden disappearance of the chief branches of the coronary arteries on the posterior surface, the nervous structure distributed over a consider­ able portion of the left ventricle is completely isolated from the blood-vessels, and on these, numerous ganglionic enlargements are likewise observed, but smaller in size than the chains of ganglia formed over the blood-vessels on the anterior surface of the heart. In the accompanying beautiful drawings, Mr. West has depicted with the greatest accuracy and minuteness the whole nervous structures demon­strable in these preparations on the surface of the heart. But the ganglia and nerves represented in these drawings constitute only a small portion of the nervous system of the heart, numerous ganglia being formed in the walls of the heart which no artist can represent. It can be clearly demonstrated that every artery distributed throughout the walls of the Uterus and Heart, and every muscular fasciculus of these organs, is supplied with nerves upon which ganglia are formed.

Bilateral Intraorbital Arachnoid Cysts of the Optic Nerves and Coloboma of the Optic Nerve Ends

1984 ◽  
Vol 4 (3) ◽  
pp. 165-168 ◽  
Author(s):  
R. Wijngaarde ◽  
G. Blaauw ◽  
A. Van Balen
Keyword(s):  
Optic Nerve ◽  
Arachnoid Cysts ◽  
Optic Nerves

scholarly journals Morphometric Analysis of Nonsclerosed Glomeruli Size and Connective Tissue Content during the Aging Process

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Vesna R. Stojanović ◽  
Ivan D. Jovanović ◽  
Sladjana Z. Ugrenović ◽  
Ljiljana P. Vasović ◽  
Vladimir S. Živković ◽  
...  
Keyword(s):  
Cluster Analysis ◽  
Connective Tissue ◽  
Morphometric Analysis ◽  
Aging Process ◽  
Systemic Disease ◽  
Digital Camera ◽  
Morphometric Parameters ◽  
Tissue Content ◽  
Tissue Area ◽  
Tissue Specimens

Number of sclerotic glomeruli increases during the aging process. Consequently, majority of remained nonsclerosed glomeruli become hypertrophic and some of them sclerotic, too. The aim of this study was to quantify the size and connective tissue content of nonsclerosed glomeruli and to evaluate the percentage of hypertrophic ones in examined human cases during the aging. Material was right kidney's tissue of 30 cadavers obtained during routine autopsies. Cadavers were without previously diagnosed kidney disease, diabetes, hypertension, or any other systemic disease. Tissue specimens were routinely prepared for histological and morphometric analysis. Images of the histological slices were analyzed and captured under 400x magnification with digital camera. Further they were morphometrically and statistically analyzed with ImageJ and NCSS-PASS software. Multiple and linear regression of obtained morphometric parameters showed significant increase of glomerular connective tissue area and percentage. Cluster analysis showed the presence of two types of glomeruli. Second type was characterized with significantly larger size, connective tissue content, and significantly lower cellularity, in relation to the first type. Such glomeruli might be considered as hypertrophic. First type of glomeruli was predominant in younger cases, while second type of glomeruli was predominant in cases older than 55 years.

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