scholarly journals Influence of the purification of human adult pancreatic islets on insulin secretion

2010 ◽  
Vol 67 (2) ◽  
pp. 128-131
Author(s):  
Dragan Nikolic ◽  
Predrag Djordjevic ◽  
Vesna Dimitrijevic-Sreckovic ◽  
Marina Dzingalasevic ◽  
Sandra Belij ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Pancreatic Islets ◽  
Density Gradient ◽  
Functional Capacity ◽  
Stimulation Index ◽  
Cellular Damage ◽  
Benign Tumors ◽  
Human Adult ◽  
Automated Method ◽  
Significant Difference

Background/Aim. The most effective method for human adult pancreatic islets purification is density-gradient centrifugation. The aim of this study was to analyze the effects of non-automated purification on preservation of functional capacity of human adult pancreatic islet cells. Methods. Human pancreata were obtained after pancreatectomy in the patients with chronic pancreatitis or benign tumors. Pancreatic islets were purified by non-automated method in discontinuous Ficoll density gradient. The samples were divided in 2 fractions: purified (P) and non-purified (NP) cultures. Islets were stained with diphenyl-thiocarbazone. The efficiency of separation was determined by comparing percentage of stained cells in P and NP cultures on day 1, 3 and 7 of shortterm cultivation. Glucose-stimulated insulin secretion was expressed as stimulation index (SI). Results. The results obtained showed a statistically significant difference (p < 0.01) between P and NP cultures. P cultures had higher percentages of stained cells (70.43 ? 3.97%, 73.77 ? 4.22% and 71.34 ? 4.69% on the first, third and seventh day of cultivation, respectively) than NP cultures (53.68 ? 1.71%, 57.14 ? 3.94% and 43.97 ? 4.56%, respectively). P cultures had higher values of SI for the first, third and seventh day of cultivation than NP cultures (0.45 ? 0.08, 0.80 ? 0.21, 1.28 ? 0.15 and 0.46 ? 0.10, 0.752 ? .0.16, 0.76 ? 0.11 for P and NP cultures respectively). The difference was statistically significant on day seven (p = 0.01). Conclusion. Although during purification process islets were exposed to a number of insults that might result in cellular damage and functional impairment, our assessments showed that islets in P cultures preserved their functional capacity better than islets in NP cultures, since they had greater insulin secretion.

scholarly journals Effect of alcohol on insulin secretion and viability of human pancreatic islets

2017 ◽  
Vol 145 (3-4) ◽  
pp. 159-164 ◽  
Author(s):  
Dragan Nikolic ◽  
Dragan Micic ◽  
Vesna Dimitrijevic-Sreckovic ◽  
Mirko Kerkez ◽  
Biljana Nikolic
Keyword(s):  
Insulin Secretion ◽  
Pancreatic Islets ◽  
Stimulation Index ◽  
Test Group ◽  
Control Group ◽  
Inadequate Response ◽  
Automated Method ◽  
Increased Risk ◽  
Significant Difference

Introduction/Objective. There are controversial data in the literature on the topic of effects of alcohol on insulin secretion, apoptosis, and necrosis of the endocrine and exocrine pancreas. The goal of this research was to determine how alcohol affects the insulin secretion and viability of human adult pancreatic islets in vitro during a seven-day incubation. Methods. Human pancreatic tissue was digested with Collagenase XI, using a non-automated method. Cultures were incubated in Roswell Park Memorial Institute (RPMI) medium containing alcohol (10 ?l of alcohol in 100 ml of medium). Insulin stimulation index (SI) and viability of the islets were determined on the first, third, and seventh day of cultivation. Results. Analysis of the viability of the islets showed that there wasn?t significant difference between the control and the test group. In the test group, viability of the cultures declined with the time of incubation. SI of the test group was higher compared to the control group, by 50% and 25% on the first and third day of cultivation, respectively. On the seventh day, insulin secretion was reduced by 25%. The difference was not statistically significant (p > 0.05). In the test group, significant decline in insulin secretion was found on the third and seventh day of incubation (p ? 0.05). Conclusion. Alcohol can increase or decrease insulin secretion of islets cultures, which may result in an inadequate response of pancreatic ?-cells to blood glucose, leading to insulin resistance, and increased risk of developing type 2 diabetes.

scholarly journals Extended time of cold ischemia and its influence on the physiological function of human adult pancreatic islets

2011 ◽  
Vol 63 (4) ◽  
pp. 1007-1013
Author(s):  
D.M. Nikolic ◽  
P.B. Djordjevic ◽  
Dj. Radak ◽  
Vesna Lackovic ◽  
Milos Bajcetic ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Pancreatic Islets ◽  
Physiological Function ◽  
Pancreatic Tissue ◽  
Physiological Solution ◽  
Cold Ischemia ◽  
Short Term ◽  
Human Pancreatic Islets ◽  
Adult Human ◽  
Human Adult

In this study we compared the effects of duration of cold ischemia (longer and shorter ischemia) on the yield, viability and preservation of the physiological function and insulin secretion of adult human pancreatic islets in short-term (seven days) culture. Based on the tested parameters, we established that there are no major differences between these two test groups and that the storage and transport of pancreatic tissue in physiological solution at 4oC gives quite satisfactory results.

Pulsatile insulin secretion from isolated human pancreatic islets

Diabetes ◽  
1994 ◽  
Vol 43 (6) ◽  
pp. 827-830 ◽  
Author(s):  
P. Marchetti ◽  
D. W. Scharp ◽  
M. Mclear ◽  
R. Gingerich ◽  
E. Finke ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Pancreatic Islets ◽  
Human Pancreatic Islets ◽  
Pulsatile Insulin Secretion

Automated method for isolation of human pancreatic islets

Diabetes ◽  
1988 ◽  
Vol 37 (4) ◽  
pp. 413-420 ◽  
Author(s):  
C. Ricordi ◽  
P. E. Lacy ◽  
E. H. Finke ◽  
B. J. Olack ◽  
D. W. Scharp
Keyword(s):  
Pancreatic Islets ◽  
Human Pancreatic Islets ◽  
Automated Method

Intraportal transplantation of pancreatic islets into livers of diabetic rats. Reinnervation of islets and regulation of insulin secretion by the hepatic sympathetic nerves

Diabetes ◽  
1994 ◽  
Vol 43 (11) ◽  
pp. 1345-1352 ◽  
Author(s):  
A. Gardemann ◽  
K. Jungermann ◽  
V. Grosse ◽  
L. Cossel ◽  
F. Wohlrab ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Pancreatic Islets ◽  
Sympathetic Nerves ◽  
Diabetic Rats

Arachidonic acid metabolism and insulin secretion by isolated human pancreatic islets

Diabetes ◽  
1988 ◽  
Vol 37 (7) ◽  
pp. 992-996 ◽  
Author(s):  
J. Turk ◽  
J. H. Hughes ◽  
R. A. Easom ◽  
B. A. Wolf ◽  
D. W. Scharp ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Insulin Secretion ◽  
Pancreatic Islets ◽  
Acid Metabolism ◽  
Arachidonic Acid Metabolism ◽  
Human Pancreatic Islets

scholarly journals Pituitary Adenylate Cyclase-Activating Polypeptide Receptors Mediating Insulin Secretion in Rodent Pancreatic Islets Are Coupled to Adenylate Cyclase But Not to PLC

Endocrinology ◽  
2002 ◽  
Vol 143 (4) ◽  
pp. 1253-1259 ◽  
Author(s):  
Francoise Jamen ◽  
Raymond Puech ◽  
Joel Bockaert ◽  
Philippe Brabet ◽  
Gyslaine Bertrand
Keyword(s):  
Insulin Secretion ◽  
Adenylate Cyclase ◽  
Pancreatic Islets ◽  
Pituitary Adenylate Cyclase

scholarly journals Vitamin B6 deficiency disrupts serotonin signaling in pancreatic islets and induces gestational diabetes in mice

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Ashley M. Fields ◽  
Kevin Welle ◽  
Elaine S. Ho ◽  
Clementina Mesaros ◽  
Martha Susiarjo
Keyword(s):  
Cell Proliferation ◽  
Insulin Secretion ◽  
Pancreatic Islets ◽  
Glucose Intolerance ◽  
Vitamin B6 ◽  
Serotonin Receptor ◽  
Dependent Manner ◽  
Β Cell ◽  
Vitamin B6 Deficiency ◽  
Maternal Glucose

AbstractIn pancreatic islets, catabolism of tryptophan into serotonin and serotonin receptor 2B (HTR2B) activation is crucial for β-cell proliferation and maternal glucose regulation during pregnancy. Factors that reduce serotonin synthesis and perturb HTR2B signaling are associated with decreased β-cell number, impaired insulin secretion, and gestational glucose intolerance in mice. Albeit the tryptophan-serotonin pathway is dependent on vitamin B6 bioavailability, how vitamin B6 deficiency impacts β-cell proliferation during pregnancy has not been investigated. In this study, we created a vitamin B6 deficient mouse model and investigated how gestational deficiency influences maternal glucose tolerance. Our studies show that gestational vitamin B6 deficiency decreases serotonin levels in maternal pancreatic islets and reduces β-cell proliferation in an HTR2B-dependent manner. These changes were associated with glucose intolerance and insulin resistance, however insulin secretion remained intact. Our findings suggest that vitamin B6 deficiency-induced gestational glucose intolerance involves additional mechanisms that are complex and insulin independent.

scholarly journals Diagnostic Accuracy of Oral Cancer and Suspicious Malignant Mucosal Changes among Future Dentists

Healthcare ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 263
Author(s):  
Kamis Gaballah ◽  
Asmaa Faden ◽  
Fatima Jassem Fakih ◽  
Anfal Yousuf Alsaadi ◽  
Nadeen Faiz Noshi ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Dental Students ◽  
Benign Tumors ◽  
Dental Practitioners ◽  
Significant Difference ◽  
Potentially Malignant Disorders ◽  
Potentially Malignant ◽  
Recent Graduates ◽  
Mucosal Changes ◽  
Oral Abnormalities

This study aimed to assess the ability of dental students and recent graduates to detect and recognize mucosal changes that are suggestive of oral cancer and potentially malignant disorders. In this cross-sectional study, a questionnaire was administered to dental students and recent graduates of Ajman University (n = 132). Completed questionnaires were received from 84 (63.6%) females and 48 (36.4%) males which included fifth-year students (n = 80), interns (n = 39), and dental practitioners (n = 13). This questionnaire was designed to assess the respondent’s ability to detect and recognize different types of oral lesions, as well as their knowledge of oral cancer appearance and malignancy potential. The overall accuracy of diagnosis was 46%. The participants correctly identified normal variations, benign tumors, malignant tumors, and premalignant lesions at rates of 60.3%, 31.0%, 55.7%, and 33.4%, respectively. There was no significant difference between the two genders in their ability to recognize and detect correct answers (females, 48.3%; males, 47.2%). According to education level, interns provided the highest percentage of correct answers (52.5%), followed by newly dental practitioners (51.9%) and fifth-year students (44.1%). Conclusion: The respondents of this survey did not exhibit a satisfactory diagnostic capability in recognizing mucosal changes consistent with the clinical presentation of oral cancer. Thus, a need exists for improved and updated educational methods for undergraduate students regarding oral cancer and potentially malignant disorders. Meanwhile, practitioners should look for oral abnormalities to provide better diagnosis and management. Practitioners should also stay up to date on the oral malignancy topic by attending workshops and clinicopathological conferences.

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