scholarly journals Extended time of cold ischemia and its influence on the physiological function of human adult pancreatic islets

2011 ◽  
Vol 63 (4) ◽  
pp. 1007-1013
Author(s):  
D.M. Nikolic ◽  
P.B. Djordjevic ◽  
Dj. Radak ◽  
Vesna Lackovic ◽  
Milos Bajcetic ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Pancreatic Islets ◽  
Physiological Function ◽  
Pancreatic Tissue ◽  
Physiological Solution ◽  
Cold Ischemia ◽  
Short Term ◽  
Human Pancreatic Islets ◽  
Adult Human ◽  
Human Adult

In this study we compared the effects of duration of cold ischemia (longer and shorter ischemia) on the yield, viability and preservation of the physiological function and insulin secretion of adult human pancreatic islets in short-term (seven days) culture. Based on the tested parameters, we established that there are no major differences between these two test groups and that the storage and transport of pancreatic tissue in physiological solution at 4oC gives quite satisfactory results.

Pulsatile insulin secretion from isolated human pancreatic islets

Diabetes ◽  
1994 ◽  
Vol 43 (6) ◽  
pp. 827-830 ◽  
Author(s):  
P. Marchetti ◽  
D. W. Scharp ◽  
M. Mclear ◽  
R. Gingerich ◽  
E. Finke ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Pancreatic Islets ◽  
Human Pancreatic Islets ◽  
Pulsatile Insulin Secretion

Arachidonic acid metabolism and insulin secretion by isolated human pancreatic islets

Diabetes ◽  
1988 ◽  
Vol 37 (7) ◽  
pp. 992-996 ◽  
Author(s):  
J. Turk ◽  
J. H. Hughes ◽  
R. A. Easom ◽  
B. A. Wolf ◽  
D. W. Scharp ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Insulin Secretion ◽  
Pancreatic Islets ◽  
Acid Metabolism ◽  
Arachidonic Acid Metabolism ◽  
Human Pancreatic Islets

SURVIVAL OF CRYOPRESERVED ISOLATED ADULT HUMAN PANCREATIC ISLETS OF LANGERHANS

1987 ◽  
Vol 44 (1) ◽  
pp. 75-82 ◽  
Author(s):  
GARTH L. WARNOCK ◽  
DEREK W. R. GRAY ◽  
PHILIP MCSHANE ◽  
MAUREEN PETERS ◽  
PETER J. MORRIS
Keyword(s):  
Pancreatic Islets ◽  
Islets Of Langerhans ◽  
Human Pancreatic Islets ◽  
Adult Human ◽  
Pancreatic Islets Of Langerhans

Deleterious Effect of Dithizone-DMSO Staining on Insulin Secretion in Rat and Human Pancreatic Islets

Pancreas ◽  
1994 ◽  
Vol 9 (2) ◽  
pp. 157-160 ◽  
Author(s):  
J. I. Conget ◽  
Y. Sarri ◽  
J. M. González-Clemente ◽  
R. Casamitjana ◽  
M. Vives ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Pancreatic Islets ◽  
Deleterious Effect ◽  
Human Pancreatic Islets

scholarly journals Pulsatile Insulin Secretion by Human Pancreatic Islets

2002 ◽  
Vol 87 (1) ◽  
pp. 213-221 ◽  
Author(s):  
Soon H. Song ◽  
Lise Kjems ◽  
Robert Ritzel ◽  
Susan M. McIntyre ◽  
Michael L. Johnson ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Pancreatic Islets ◽  
Human Pancreatic Islets ◽  
Pulsatile Insulin Secretion

Activin A stimulates insulin secretion in cultured human pancreatic islets

2000 ◽  
Vol 23 (4) ◽  
pp. 231-234 ◽  
Author(s):  
P. Florio ◽  
S. Luisi ◽  
P. Marchetti ◽  
R. Lupi ◽  
L. Cobellis ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Pancreatic Islets ◽  
Activin A ◽  
Human Pancreatic Islets

scholarly journals Influence of the purification of human adult pancreatic islets on insulin secretion

2010 ◽  
Vol 67 (2) ◽  
pp. 128-131
Author(s):  
Dragan Nikolic ◽  
Predrag Djordjevic ◽  
Vesna Dimitrijevic-Sreckovic ◽  
Marina Dzingalasevic ◽  
Sandra Belij ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Pancreatic Islets ◽  
Density Gradient ◽  
Functional Capacity ◽  
Stimulation Index ◽  
Cellular Damage ◽  
Benign Tumors ◽  
Human Adult ◽  
Automated Method ◽  
Significant Difference

Background/Aim. The most effective method for human adult pancreatic islets purification is density-gradient centrifugation. The aim of this study was to analyze the effects of non-automated purification on preservation of functional capacity of human adult pancreatic islet cells. Methods. Human pancreata were obtained after pancreatectomy in the patients with chronic pancreatitis or benign tumors. Pancreatic islets were purified by non-automated method in discontinuous Ficoll density gradient. The samples were divided in 2 fractions: purified (P) and non-purified (NP) cultures. Islets were stained with diphenyl-thiocarbazone. The efficiency of separation was determined by comparing percentage of stained cells in P and NP cultures on day 1, 3 and 7 of shortterm cultivation. Glucose-stimulated insulin secretion was expressed as stimulation index (SI). Results. The results obtained showed a statistically significant difference (p < 0.01) between P and NP cultures. P cultures had higher percentages of stained cells (70.43 ? 3.97%, 73.77 ? 4.22% and 71.34 ? 4.69% on the first, third and seventh day of cultivation, respectively) than NP cultures (53.68 ? 1.71%, 57.14 ? 3.94% and 43.97 ? 4.56%, respectively). P cultures had higher values of SI for the first, third and seventh day of cultivation than NP cultures (0.45 ? 0.08, 0.80 ? 0.21, 1.28 ? 0.15 and 0.46 ? 0.10, 0.752 ? .0.16, 0.76 ? 0.11 for P and NP cultures respectively). The difference was statistically significant on day seven (p = 0.01). Conclusion. Although during purification process islets were exposed to a number of insults that might result in cellular damage and functional impairment, our assessments showed that islets in P cultures preserved their functional capacity better than islets in NP cultures, since they had greater insulin secretion.

scholarly journals Human placental lactogen (hPL-A) activates signaling pathways linked to cell survival and improves insulin secretion in human pancreatic islets

Islets ◽  
2011 ◽  
Vol 3 (5) ◽  
pp. 250-258 ◽  
Author(s):  
Marco F. Lombardo ◽  
Fabiana De Angelis ◽  
Luca Bova ◽  
Barbara Bartolini ◽  
Federico Bertuzzi ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Cell Survival ◽  
Pancreatic Islets ◽  
Signaling Pathways ◽  
Placental Lactogen ◽  
Human Placental Lactogen ◽  
Human Pancreatic Islets
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