scholarly journals Histometric analysis of gingival hyperplasia in Wistar rats during nifedipine administration

2007 ◽  
Vol 64 (1) ◽  
pp. 19-23
Author(s):  
Zlata Brkic
Keyword(s):  
Wistar Rats ◽  
Water Solution ◽  
Control Group ◽  
Channel Blockers ◽  
Gingival Hyperplasia ◽  
Time Intervals ◽  
Tissue Samples ◽  
Experimental Animals ◽  
Periodontal Tissues ◽  
Interdental Papilla

Background/Aim. The use of calcium channel blockers, especially nifedipine, causes gingival hyperplasia which leads to the destruction of the deeper periodontal tissues. During this process, inflammatory changes and the changes of colagen fibers occur. The aim of this study was to metrically compare the extent of proliferation of connective tissue in the deeper periodontal tissue in experimental animals regarding the dose and duration of nifedipine administration. Methods. The study involved 50 Wistar rats to which water solution of nifedipine was given in certain time intervals and doses. Before starting the experiment, i.e. before nifedipine administration, and in the defined time intervals, measuring of the morphology of gingival size was performed including the buccolingual and mesiodistal wideness and vertical altitude of the central interdental papilla. The measurement was performed by the use of a special graduated probe. Histometric analyses of the tissue samples were done on the sagital cross-sections in the direction from the top to the bottom of papilla on five levels. For the statistical analysis of the data, the established values to the extent of the most present changes were used. The mesiodistal and buccolingual diameters for the levels L2 and L3 were quantitively determined and compared. These values were compared to the vertical diameter of gingival growth determined before the onset of patohistologic analyses of the tissue samples. Results. At the beginning of the experiment, the volume of the lower incisive central papilla in the rats was 12 mm3. The central interdental papilla vertical altitude was 6.6 mm in rats which had received a lower dose of nifedipine, 8 mm in rats which had received a higher dose in the defined time intervals while the value for the control group was 3.8 mm. Conclusion. The obtained results showed that the administration of nifedipine led to the extensive gingival hyperplasia in the experimental animals. Gingival hyperplasia correlates with both the dose of nifedipine and the duration of its administration.

scholarly journals Influence of nifedipine on gingiva of Wistar rats

2004 ◽  
Vol 61 (1) ◽  
pp. 5-8 ◽  
Author(s):  
Zlata Brkic
Keyword(s):  
Wistar Rats ◽  
Water Solution ◽  
Channel Blockers ◽  
Gingival Hyperplasia ◽  
Dental Practice ◽  
Gingival Fibroblasts ◽  
Gingival Overgrowth ◽  
Time Period ◽  
Time Periods ◽  
Higher Doses

Noninflammatory hyperplastic growth of gingiva induced by calcium channel blockers, mostly nifedipine, is often seen in everyday dental practice. In order to establish an association of nifedipine and gingival hyperplasia, experimental model was used. Wistar rats were given water solution of nifedipine in different daily doses, using specially designed cannula. At the beginning of the experiment, before the application of nifedipine and in the determined time periods, gingival volume was measured. The volume of lower incisors interdental central papillas, represented multiplied values of vertical hight, mesio-distal width, and bucco-lingual depth, expressed in millimeters. The results indicated that gingival hyperplasia was more excessive in the experimental animals, which were given higher doses of the drug for longer time period. Nifedipine is a drug which induces gingival fibroblasts to produce higher quantity of collagen that causes gingival overgrowth.

scholarly journals Histopathologic investigations of nifedipine-induced gingival hyperplasia in Wistar rats

2005 ◽  
Vol 62 (3) ◽  
pp. 207-211 ◽  
Author(s):  
Zlata Brkic
Keyword(s):  
Cross Sections ◽  
Wistar Rats ◽  
Alveolar Bone ◽  
Bone Destruction ◽  
Extended Period ◽  
Gingival Hyperplasia ◽  
Experimental Animals ◽  
Periodontal Tissues ◽  
Periodontal Destruction ◽  
High Doses

Background. Nifedipine, a calcium channel blocker, used in the therapy of cardiovascular diseases, can induce gingival overgrowth. The aim of the study was to assess the influence of nifedipine on the deeper periodontal tissues of the experimental animals concerning connective tissue and blood vessels proliferation. Methods. The research was performed on Wistar rats, and they received the precise doses of nifedipine water suspension while the changes were followed up in pre-determined time intervals. The analyses of samples of tissues were done on the cross-sections of papilla from the top to the bottom on five levels. Results. Considerable level of changes was observed in the area of dental alveolar edge and in the gingivo-dental interspace. Epithelial hyperplasia and inflammatory subepithelial infiltration of lymphoplasmocyte type were also present. Alveolar bone destruction, the increased number of osteoclasts and periodontal destruction were found, as well. Conclusion. The results of this study showed a significant gingival hyperplasia in the experimental animals which received high doses of drug during extended period of time.

scholarly journals ASSESSMENT OF IMMUNOMODULATORY POTENTIAL OF AN AYURVEDIC FORMULATION, NIROCIL SYRUP IN WISTAR RATS

2020 ◽  
pp. 154-158
Author(s):  
DINESH DILIP GHADIGAONKAR ◽  
MUKESH B CHAWDA ◽  
KAPIL S THAKUR
Keyword(s):  
Wistar Rats ◽  
Animal Ethics ◽  
Complete Blood Count ◽  
Treated Group ◽  
Control Group ◽  
Differential Leukocyte Count ◽  
Experimental Animals ◽  
Prior Approval ◽  
Hemagglutination Titer ◽  
Immunomodulatory Potential

Objective: This study aims to assess the immunomodulatory potential of an Ayurvedic formulation, Nirocil syrup, in Wistar rats. Methods: The experiments were conducted on Wistar rats with prior approval from the Institutional Animal Ethics Committee. Nirocil syrup was administered for 6 weeks to experimental animals. Parameters such as hemagglutination titer, histopathology of immunological organs, complete blood count, differential leukocyte count, and immunological paw edema were recorded and compared with controlled (untreated) and becozinc treated groups. Results: Nirocil treated group significantly enhanced the antibody titer in comparison to the control group. The results are supported by the increase in blood lymphocyte count and antigenic stimulation in immunological organs (spleen). Nirocil syrup enhanced antibody formation and suppressed the immunological edema in experimental animals. Conclusions: The study concludes that the Ayurvedic formulation Nirocil syrup has immunopotentiating activity.

scholarly journals Microanatomical and biochemical changes of the cerebellum following ethanol gavage in adult Wistar rats

2019 ◽  
Vol 8 (2) ◽  
pp. 1662-1669
Author(s):  
I.M. Usman ◽  
I.A. Iliya ◽  
A.E. Ivang ◽  
F Ssempijja ◽  
A.O. Ojewale ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Sialic Acid ◽  
Purkinje Cells ◽  
Wistar Rats ◽  
Histological Study ◽  
Ethanol Exposure ◽  
Biochemical Changes ◽  
Control Group ◽  
Experimental Animals ◽  
Dose Dependent

Ethanol consumption has been linked with social and medical problems, coupled with damage of multiple organs including the cerebellum. The present study is aimed at investigating the histological and biochemical changes in the cerebellum of Wistar rats associated with ethanol exposure. The experimental animals were grouped into five groups designated as Group 1 which served as the control group and was given distilled water, Groups 2,3,4 and 5were given 40%, 25%, 12% and 5% v/v of ethanol respectively. Each of the experimental animals was administered 10mls/kg body weight of the stock solution for 42days after which the animals were sacrificed humanely. The cerebellum was removed, fixed in Bouins fluid for histological study while brain homogenates were prepared and used for the biochemical studies. Data was analyzed using one-way ANOVA and Tukey HSD Post-Hoc comparison test was used to determine where the difference lies. Oxidative stress studies showed significant increase and decrease in some oxidative stress markers when compared to the control group (p<0.05). The sialic acid studies showed a dose dependent decrease in the mean sialic acid concentration of the cerebellum across the groups when compared to the control (p<0.05). The histological studies showed the following changes; necrotic Purkinje cells with reduced linear distribution of Purkinje cells, in section of the cerebellar tissue of rats in Groups 2 and 3 with sections from Groups 4 and 5 remaining relatively normal when compared to the slide from the control group. Exposure to ethanol from the present studies showed a dose dependent effect on the cerebellum, as manifested in the histological and biochemical studies.Keywords: Ethanol gavage, Histological, Biochemical changes, Cerebellum

scholarly journals Dynamics of blood morphological indicators of Wistar line rats under parenteral BLV infection

2021 ◽  
pp. 53-58
Author(s):  
A. V. Krasnikov ◽  
A. S. Belyakova ◽  
E. S. Krasnikova
Keyword(s):  
Aplastic Anemia ◽  
Wistar Rats ◽  
Lymphocytic Leukemia ◽  
Control Group ◽  
Experimental Animals ◽  
Laboratory Rats ◽  
Intraperitoneal Infection ◽  
Experimental Group

Hematological studies of Wistar rats with intraperitoneal infection of their lymphocytes from BLV-infected cows revealed markers characteristic of the leukemic process induced by the pathogen enzootic leukemia in cattle. In 75% of experimental animals, lymphocytic leukemia and neutropenia were detected. The number of lymphocytes in the blood of rats of the experimental group was 17-36 % more than in the control group, leukocytes in average by 30 %. The animals of the experimental group showed signs of erythrocyte aplasia, hemolytic or aplastic anemia. Allergy markers were observed in individual rats. This allows us to recommend an intraperitoneal method of infecting laboratory rats with suspended lymphocytes from infected livestock for rapid and informative reproduction of experimental BLV infection.

scholarly journals Histopathological Changes in Gastrointestinal Tissues of Wistar Rats Administered with Methanolic Leaf Extract of Caladium bicolor (Araceae)

2020 ◽  
pp. 12-19
Author(s):  
Dayo Rotimi Omotoso ◽  
Adeniran Oluwadamilare Akinola ◽  
Ibifuro Brown
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Body Weight Loss ◽  
The Body ◽  
Control Group ◽  
Surface Erosion ◽  
Histopathological Changes ◽  
Experimental Animals ◽  
Toxic Potential

To assess the effect of methanolic leaf extract of Caladium bicolor on the histomorphology of gastrointestinal tissues of experimental animals. Twenty four Wistar rats (weighing between 175-190 g) were randomly and equally divided into four groups which include one control group (CG) and three treatment groups (TG I, TG II and TG III). The CG was administered with distilled water [2 ml/kg body weight (b.w.)] while TGs I, II and III were administered with 100 ml/kg, 200 ml/kg and 300 ml/kg (b.w.) of C. bicolor extract respectively. All administrations were done orally and once daily for a period of thirty days. The body weight of all animals was recorded at the beginning and end of study. After the period of study, gastric and small intestinal tissues of experimental animals were harvested, processed, converted to tissue blocks and sectioned. Tissue sections were stained using Haematoxylin and Eosin (H&E) technique. Thereafter, stained sections microscopically examined for observable histopathological changes within study tissues. The results of this study showed that exposure to C. bicolor extract causes significant (p < 0.05) body weight loss in TGs I-III compared to CG. In addition, prominent histopathological changes were observed in gastrointestinal tissues of experimental animals in TGs I-III including gastric mucosal surface erosion and intestinal villi degeneration compared to normal gastrointestinal histomorphology of CG animals. These histopathological changes may be associated with toxic effect of phytochemicals constituents of the extract. Therefore, its application for therapeutic purposes needs to be thoroughly re-validated or perhaps disallowed where alternative therapeutic agents with minimal toxic potential exist.

scholarly journals Study of acute hepatotoxicity of Equisetum arvense L. in rats

2009 ◽  
Vol 24 (6) ◽  
pp. 449-453 ◽  
Author(s):  
Nilo César do Vale Baracho ◽  
Bruno Battiston Vilela Vicente ◽  
Guilherme D'Andréa Saba Arruda ◽  
Brunno Cezar Framil Sanches ◽  
Jarbas de Brito
Keyword(s):  
Wistar Rats ◽  
Oral Treatment ◽  
The Other ◽  
Hepatic Tissue ◽  
Control Group ◽  
Equisetum Arvense ◽  
Hepatic Enzymes ◽  
Tissue Samples ◽  
Blood Samples ◽  
Lobular Structure

PURPOSE: To evaluate the acute hepatotoxicity of Equisentum arvense L. in rats. METHODS: Fifty Wistar rats were used, these being divided in four groups, one being the control (receiving only water) and the other groups receiving graded doses of Equisentum arvense L. (30, 50, and 100mg/kg respectively) for 14 days. Blood samples were obtained to determine TGO, TGP, FA, DHL and GT-gamma activities. After that, hepatic tissue samples were collected for the anatomopathologic analysis. RESULTS: The anatomopathologic exam of the hepatic tissue showed organ with preserved lobular structure. In the same way, there was no significant change in the seric activities of the hepatic enzymes when compared to control group. CONCLUSION: The oral treatment with graded doses of Equisentum arvense L. was not able to produce hepatic changes. Further studies are necessary to evaluate the chronic hepatotoxicity of Equisentum arvense L. in rats.

scholarly journals Acute Toxicity Study of Iron-21 Syrup in Female Wistar Rats

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 2637-2643
Author(s):  
Kirti G Sahu ◽  
Manish P Deshmukh ◽  
Sukeshini B Lote ◽  
Ashish B Budrani ◽  
Deepak S Khobragade
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Oral Route ◽  
The Body ◽  
Control Group ◽  
Haematological Parameter ◽  
Oral Toxicity ◽  
Tissue Samples ◽  
Histopathological Studies

The iron-21 syrup is used for iron deficiency anaemia which supplies iron and calories a provide iron and calorie nutriment to recompense haemoglobin deprivation. The objective of this study is to determine acute oral toxicity of Iron-21 syrup in vivo in Wistar rats. Iron-21 Syrup formulation was given through the oral route. The syrup formulation was administered in three increasing doses of 3, 6 and 12 ml/kg body weight for concentration of 500, 1000 and 2000mg/kg respectively. The other group of rats designated as a control group was given the only vehicle orally. The test and control group contains five rats each. Tests, as well as control group rats, were sacrificed on the fifteenth day of treatment. The blood and tissue samples of test animals were sent for histopathological studies examination. Four parameters were observed throughout the study, and they are cage side observation, the effect to the body weight, haematological parameter and histopathology. All animals were survived till they sacrificed. No notable changes were found in behaviour, haematological and histopathology studies. The oral administration of Iron-21 Syrup is not shown any toxic effect in the animal at a given dose. Therefore, it is a safe remedy for human use.

scholarly journals The oxygen-induced retinopathy as an experimental model of retinopathy of prematurity

2013 ◽  
Vol 6 (3) ◽  
pp. 37-42 ◽  
Author(s):  
Olga Aleksandrovna Konikova ◽  
Vladimir Vsevolodovich Brzheskiy ◽  
Yelena Pavlovna Fedotova ◽  
Ruslan Abdulayevich Nasyrov
Keyword(s):  
Experimental Model ◽  
Retinopathy Of Prematurity ◽  
Wistar Rats ◽  
Clinical Signs ◽  
Control Group ◽  
Experimental Animals ◽  
Oxygen Induced Retinopathy ◽  
Significant Impairment ◽  
Experimental Group

The experimental model of retinopathy of prematurity was developed on the base of an oxygen-induced retinopathy in newborn. Wistar rats. This model was meant to investigate histopathological and functional manifestations of the disease. The study was performed on 60 newborn Wistar rats. The main experimental group included 34 animals with induced retinopathy of prematurity, the control group — 26 experimental animals. The predominating morphological manifestations of the oxygen-induced retinopathy were photoreceptor apoptosis, and the development of pathological intraretinal vascularization. Histological and electrophysiological changes were also detected even before the formation of clinical signs of retinopathy. There was a significant impairment of immature retina architectonic after induced hyperoxia.

scholarly journals Effects Of Amitryptilin Administration on Rat Sera and Brain Beta-endorphins

2006 ◽  
Vol 6 (4) ◽  
pp. 64-66 ◽  
Author(s):  
Radivoj Jadrić ◽  
Sabaheta Hasić ◽  
Emina Kiseljaković ◽  
Jovan Radovanović ◽  
Emina Ićindić-Nakaš ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Antidepressant Drug ◽  
Drug Effects ◽  
Control Group ◽  
Psychoactive Drugs ◽  
Experimental Animals ◽  
Antidepressive Drugs ◽  
Beta Endorphins

The aim of our study was to establish the influence of antidepressive drugs on serum and brain beta-endorphins in experimental animals. Experiment was performed on albino Wistar rats. Antidepressant amitryptiline was used, and for quantification of sera and brain beta-endorphins RIA technique. Our results showed difference between sera and brain beta-endorphins concentration in amitryptiline pretreated animals, vs. those in serum and brain of control group treated with 0.95% NaCl. This study shows that use of psychoactive drugs have influence on sera and brain beta-endorphins concentration. Beta-endorphins could be of great importance, used as markers for evaluation of antidepressant drug effects.

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