scholarly journals Plum pox virus infection level in Prunus species growing along roadsides or in backyards in Vojvodina province

2021 ◽  
Vol 36 (3) ◽  
pp. 111-118
Author(s):  
Ferenc Bagi ◽  
Goran Barac ◽  
Renata Ilicic ◽  
Zagorka Savic ◽  
Milan Burmazovic ◽  
...  
Keyword(s):  
Life Stage ◽  
Sour Cherry ◽  
Early Life Stage ◽  
Plum Pox Virus ◽  
Virus Infections ◽  
Infection Rates ◽  
Prunus Species ◽  
Infection Level ◽  
Novi Sad ◽  
Vojvodina Province

The present study was conducted on 106 leaf samples of Prunus species (44 plum, 47 myrobalan plum, 10 apricot, 3 peach, 1 blackthorn, and 1 sour cherry) collected from the Vojvodina province of Serbia and assessed for the presence of Plum pox virus (PPV) with the aim of establishing whether trees growing along roadsides and in backyards could be sources of PPV infection in commercial orchards. Analyses confirmed PPV infection in 68.2%, 38.6%, and 20% of plum, myrobalan plum and apricot samples, respectively. The infection level varied considerably across locations, with 80% noted in Uljma, followed by 69% in Sremska Kamenica, 60% in Becej, 50% in Temerin, 50% in Senta, 40% in Petrovaradin, 31% in Novi Sad, 30% in Susek, and 20% in Subotica. As no difference in infection rates was noted between young and older plum trees, the infections seemed to have occurred in their early life stage. Trees growing along roadsides were infected to a higher extent (89.6%) than those in backyards (66.6%). In some cases, symptomless trees were also proven to be PPV positive, indicating that they are a potentially important source of further virus infections. Due to the widespread presence of the virus, tolerant or hypersensitive cultivars are recommended for planting new orchards.

scholarly journals Prunus Host Range of Plum pox virus (PPV) in the United States by Aphid and Graft Inoculation

Plant Disease ◽  
2007 ◽  
Vol 91 (1) ◽  
pp. 18-23 ◽  
Author(s):  
V. D. Damsteegt ◽  
R. Scorza ◽  
A. L. Stone ◽  
W. L. Schneider ◽  
K. Webb ◽  
...  
Keyword(s):  
Virus Disease ◽  
Systemic Infection ◽  
Sour Cherry ◽  
Aphid Transmission ◽  
The United States ◽  
Enzyme Linked Immunosorbent Assay ◽  
Plum Pox Virus ◽  
Rt Pcr ◽  
Prunus Species ◽  
Wide Range

Plum pox (Sharka) is a serious virus disease of stone fruits caused by the Plum pox virus (PPV). To determine which species could function as potential hosts and virus reservoirs, we used aphid transmission and bud or chip grafting to evaluate the susceptibility of commercial, ornamental, and wild Prunus species to isolates of PPV found in Pennsylvania, USA. Following inoculation, test trees were observed for symptoms, analyzed by enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR), back-assayed to healthy peach, and followed through at least four cold-induced dormancy (CID) cycles over 4 years. Thirty-one of 33 Prunus species and cultivars were systemically infected following aphid transmission. Systemic infection could not be detected in P. cerasus (sour cherry) and P. × ‘Snofozam’ (Snow Fountains) despite repeated aphid inoculation attempts. Following grafting of PPV-infected budwood, all 40 species and varieties became infected, although species differed in their susceptibility. Within most species, some individual plants remained PPV negative throughout the study despite repeated inoculations. Infection in some species could be detected only through quantitative reverse transcription (RT)-PCR. Most species displayed clear symptoms, were highly positive by ELISA and RT-PCR, and could be back-inoculated into peach seedlings following CID. Our results indicate that a wide range of native and ornamental Prunus species are susceptible to U.S. isolates of PPV-D.

Age and growth of early life stage Atlantic Tarpon Megalops atlanticus from the northcentral Gulf of Mexico

2021 ◽  
Author(s):  
Patrick M. Graham ◽  
James S. Franks ◽  
Evan J. Anderson ◽  
Robert T. Leaf ◽  
Jason D. Tilley
Keyword(s):  
Gulf Of Mexico ◽  
Early Life ◽  
Life Stage ◽  
Early Life Stage ◽  
Age And Growth ◽  
Megalops Atlanticus ◽  
Atlantic Tarpon

scholarly journals Reproductive strategy, egg characteristics and embryonic development of Greenland halibut (Reinhardtius hippoglossoides)

2012 ◽  
Vol 70 (2) ◽  
pp. 342-351 ◽  
Author(s):  
Rosario Domínguez-Petit ◽  
Patrick Ouellet ◽  
Yvan Lambert
Keyword(s):  
Embryonic Development ◽  
Reproductive Strategy ◽  
Biochemical Composition ◽  
Incubation Temperature ◽  
Life Stage ◽  
Early Life Stage ◽  
Greenland Halibut ◽  
Hatching Time ◽  
Greenland Halibut Reinhardtius Hippoglossoides ◽  
Reinhardtius Hippoglossoides

Abstract Domínguez-Petit, R., Ouellet, P., and Lambert, Y. 2013. Reproductive strategy, egg characteristics and embryonic development of Greenland halibut (Reinhardtius hippoglossoides). – ICES Journal of Marine Science, 70: 342–351. Despite the commercial importance of Greenland halibut (GH), important gaps exist in our knowledge of the reproductive and early life stage biology for this species. The present study examined through laboratory experiments the spawning strategy, realized fecundity, egg characteristics, biochemical composition, and embryonic development of GH. The results confirmed the hypothesis that GH is a single-batch spawner producing large eggs, resulting in low realized fecundity. Embryonic development and hatching time are highly dependent on incubation temperature; 50% hatching occurred after 46, 30, and 24 days at 2, 4, and 6°C, respectively. Few changes in the biochemical composition of the eggs are observed during embryonic development. Newly hatched larvae are not well developed, having a large yolk sac, no pigmentation and incomplete development of the jaws. Egg specific density confirmed the mesopelagic distribution of the eggs at sea. However, important buoyancy changes occurring in the last 3–4 days before hatching indicate that larvae hatch higher in the water column. These results are important for understanding advection and dispersion processes of GH eggs and larvae and the connectivity between spawning grounds and nursery areas.

Transcriptomic and phytohormonal analysis of the interaction between Plum pox virus (PPV, sharka) and Prunus species

2017 ◽  
pp. 295-298
Author(s):  
M. Rubio ◽  
P.M. Olivares ◽  
M.A. Batnini ◽  
F. Dicenta ◽  
P. Martínez-Gómez
Keyword(s):  
Plum Pox Virus ◽  
Prunus Species
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