scholarly journals Carbapenems resistance of Acinetobacter spp strains isolated from wound swabs during 2009-2010

2011 ◽  
Vol 64 (11-12) ◽  
pp. 583-587 ◽  
Author(s):  
Deana Medic ◽  
Mira Mihajlovic-Ukropina ◽  
Vera Gusman ◽  
Zora Jelesic ◽  
Biljana Milosavljevic
Keyword(s):  
Continuous Monitoring ◽  
Hospital Setting ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Great Ability ◽  
Clinical Center ◽  
Acinetobacter Spp ◽  
Resistant Strains ◽  
Novi Sad ◽  
And Control

Introduction. Acinetobacter spp has become an important cause of nosocomial infections due to its great ability to survive and spread in a hospital setting and to develop resistance to many antibiotics. The aim of this study was to examine the resistance to carbapenems and other commonly used antibiotics in strains of Acinetobacter isolated from wound swabs. Material and Methods. In the Laboratories of Microbiology Center at the Institute for Public Health in Novi Sad wound swabs were taken from the patients hospitalized at the Institutes and Departments of the Clinical Center of Vojvodina in Novi Sad. A total of 271 Acinetobacter spp strains were tested to susceptibility on carbapenems and other most commonly used antibiotics by disk diffusion method according to the recommendation of the Clinical and Laboratory Standards Institute. Results and Discussion. Acinetobacter spp (271 isolates) developed resistance to imipenem and meropenem (67.4% and 64.4%). The resistance to both cephalosporins III and IV generation and ciprofloxacin was 100%. The resistance to ampicillin-sulbactam was 71.4%. Conclusion. Our results show high resistance to carbapenems in Acinetobacter spp strains isolated from wound swabs. These facts suggest the need for continuous monitoring of susceptibility in order to take adequate measures to prevent and control spreading of resistant strains.

scholarly journals Cockroaches as a Source of High Bacterial Pathogens with Multidrug Resistant Strains in Gondar Town, Ethiopia

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Feleke Moges ◽  
Setegn Eshetie ◽  
Mengistu Endris ◽  
Kahsay Huruy ◽  
Dagnachew Muluye ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
Bacterial Species ◽  
Multidrug Resistant ◽  
Resistance Rate ◽  
Control Measures ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Gondar Town ◽  
Resistant Strains ◽  
And Control

Background. Cockroaches are source of bacterial infections and this study was aimed to assess bacterial isolates and their antimicrobial profiles from cockroaches in Gondar town, Ethiopia.Methods. A total of 60 cockroaches were collected from March 1 to May 30, 2014, in Gondar town. Bacterial species were isolated from external and internal parts of cockroaches. Disk diffusion method was used to determine antibiotic susceptibility patterns. Data were entered and analyzed by using SPSS version 20;Pvalues <0.005 were considered as statistically significant.Results. Of 181 identified bacteria species, 110 (60.8%) and 71 (39.2%) were identified from external and internal parts of cockroaches, respectively.Klebsiella pneumoniae32 (17.7%),Escherichia coli29 (16%), andCitrobacterspp. 27 (15%) were the predominant isolates. High resistance rate was observed to cotrimoxazole, 60 (33.1%), and least resistance rate was noted to ciprofloxacin, 2 (1.1%). Additionally, 116 (64.1%) of the isolates were MDR strains;Salmonellaspp. were the leading MDR isolates (100%) followed byEnterobacter(90.5%) andShigellaspp. (76.9%).Conclusion. Cockroaches are the potential source of bacteria pathogens with multidrug resistant strains and hence effective preventive and control measures are required to minimize cockroach related infections.

scholarly journals Dynamics of resistance of Streptococcus pneumoniae strains to antibacterial drugs for the period 2015–2019 in Samara: opportunities for overcoming

2020 ◽  
Vol 30 (2) ◽  
pp. 219-226
Author(s):  
S. S. Sobina ◽  
M. O. Zolotov ◽  
A. V. Lyamin ◽  
D. D. Ismatullin ◽  
A. V. Zhestkov
Keyword(s):  
Streptococcus Pneumoniae ◽  
Age Groups ◽  
Statistical Processing ◽  
World Health ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Antibacterial Drugs ◽  
Low Level ◽  
Study Results ◽  
Resistant Strains

According to the World Health Organization (WHO), diseases caused by Streptococcus pneumoniae are one of the most dangerous diseases, that can be completely prevented by vaccination. Studies conducted in various regions of the Russian Federation and other countries have revealed certain features in the prevalence of pneumococcus strains, resistant to different antibacterial drugs, which requires local studies among patients of different age groups.The aim of the study was to determine the level of sensitivity of S. pneumoniae, isolated from adult patients, to antibacterial drugs (ABD) and the dynamics of resistance to ABD in Samara for the period of 2015–2019.Methods. A retrospective analysis of data from the microbiological department of clinical diagnostic laboratories of the Clinics of Samara State Medical University, Healthcare Ministry of Russia for 2015–2019 was carried out. The microorganism resistance to several groups of ABD was studied – macrolides (erythromycin, azithromycin, clarithro mycin), cephalosporins (ceftriaxone, cefotaxime), fluoroquinolones (levofloxacin) and penicillin. The sensitivity was determined by the disk diffusion method. Statistical processing was performed using the SPSS Statistic 22.0 program. When comparing groups, the χ2 criterion was used; to compare small groups (n < 5), the exact Fisher test was used.Results. Since 2017, there has been a tendency to increase the frequency of pneumococcus isolation in the carrier group. At the same time, a 3-fold decrease in S. pneumoniae excretion from patients with ENT pathology occurred. The highest sensitivity was detected for cephalosporins – 100% sensitivity of all S. pneumoniae strains, detected from ENT organs and during carriage. There is a low level of pneumococcus resistance to levofloxacin. The general sensitivity to ABD from penicillin group is 96.4–98.8%, while the most common resistant strains were found in patients with respiratory tract damage. The highest resistance of S. pneumoniae was revealed to ABD from the macrolide group with a tendency to increase from 4.6% in 2016 to 14.0% in 2019.Conclusion. According to the study results, a rather low level of resistance of S. pneumoniae to most ABD groups, with the exception of macrolides, was demonstrated in Samara. The greatest number of ABD-insensitive pathogens was isolated in patients with pathology of the bronchopulmonary system. A mandatory microbiological examination of the sputum of such patients is necessary with a determination of the sensitivity of the isolated microorganisms to ABD and subsequent correction of therapy upon the identification of resistant strains. Given the increasing resistance of S. pneumoniae in carriers, it is necessary to vaccinate patients in this group. 

scholarly journals Prevalence and Characteristics of Metallo-beta Lactamase-positive and High-risk Clone ST235 Pseudomonas aeruginosa at Ardabil Hospitals

2021 ◽  
Vol 14 (3) ◽  
Author(s):  
Somayeh Safarirad ◽  
Mohsen Arzanlou ◽  
Jafar Mohammadshahi ◽  
Hamid Vaez ◽  
Amirhossin Sahebkar ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Continuous Monitoring ◽  
Multidrug Resistant ◽  
Resistance Rate ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Carbapenem Resistant ◽  
Clonal Relatedness ◽  
Synergy Test ◽  
Highly Virulent

Background: Carbapenems are the most commonly administered drugs for the treatment of multidrug-resistant Pseudomonas aeruginosa (MDR-P. aeruginosa) infections. However, carbapenem-resistant P. aeruginosa is spreading rapidly and has led to a new threat to human health worldwide. Objectives: The current study aimed to determine the prevalence of imipenem-resistant P. aeruginosa, detect metallo-β-lactamase (MBL)-producer isolates, and evaluate their clonal relationships in strains isolated from patients referring to the hospitals of Ardabil city, Iran. Methods: The resistance rate to imipenem was evaluated using the disk diffusion method. Double-disk synergy test and PCR technique were used for phenotypic and genotypic screening of MBL-positive P. aeruginosa, respectively. Ultimately, ERIC-PCR and MLST methods were used for assessing clonal relatedness among the isolates. Results: The prevalence of imipenem-resistant P. aeruginosa strains was estimated at 57.1% (48 out of 84 isolates). In addition, 45 (93.7%) out of 48 imipenem-resistant P. aeruginosa isolates were phenotypically screened as MBL-positive, among which 16 (35.5%) and three (6.6%) isolates harbored blaIMP and blaVIM-1 genes, respectively. However, blaNDM, blaSIM-2, blaSPM, and blaGIM-1 genes were not detected in this study. MBL-producing P. aeruginosa strains were divided into 42 ERIC-PCR types. Based on the results of MLST, P. aeruginosa ST235 was the only identified sequence type. Conclusions: Our results revealed a high and alarming prevalence of imipenem-resistant and blaIMP-positive P. aeruginosa ST235 at Ardabil hospitals. Continuous monitoring is essential to control the further spread of this highly virulent and drug-resistant clone.

scholarly journals Antifungal Susceptibility of Malassezia pachydermatis Isolates from Dogs

2019 ◽  
Vol 63 (2) ◽  
pp. 15-20
Author(s):  
Z. Sihelská ◽  
E. Čonková ◽  
P. Váczi ◽  
M. Harčárová
Keyword(s):  
Antifungal Agents ◽  
Antifungal Susceptibility ◽  
Diffusion Method ◽  
Opportunistic Pathogens ◽  
Ear Canal ◽  
Disk Diffusion Method ◽  
Malassezia Pachydermatis ◽  
Resistant Strains ◽  
Standard Disk

Abstract The genus Malassezia belongs to Basidiomycota and includes 16 species, from which M. pachydermatis is the most common in dogs. M. pachydermatis is a member of the normal mycobiota of the skin and mucosal sites of dogs. Under certain conditions, these yeasts can be opportunistic pathogens and involved skin and ear canal infections of these animals. Topical and oral antifungal agents are used for the therapy of Malassezia dermatitis and otitis. With the expanding use of antifungal agents, resistant strains of Malassezia are increasingly detected. In this study, the susceptibility of 40 M. pachydermatis isolates to fluconazole, itraconazole, ketoconazole, clotrimazole and nystatin were evaluated in vitro based on the modified standard disk diffusion method M44-2A.

scholarly journals Effects of introducing the improved energy management system in the Urgent Care Center of the Clinical Center of Vojvodina

2019 ◽  
Vol 23 (5 Part B) ◽  
pp. 2919-2927
Author(s):  
Djura Oros ◽  
Velimir Congradac ◽  
Veran Vasic ◽  
Filip Kulic
Keyword(s):  
Energy Efficiency ◽  
Energy Consumption ◽  
Care Center ◽  
Hot Water ◽  
Urgent Care ◽  
Urgent Care Center ◽  
Energy Demands ◽  
Clinical Center ◽  
Novi Sad ◽  
And Control

The Urgent Care Center of the Clinical Center of Vojvodina in city of Novi Sad, Republic od Serbia, as a specific medical facility with very demanding conditions of work and functioning, has an extensive and constant energy demands. The building is new, set into operation in 2010. The state-of-the-art concept and the equipment installed create conditions for using advanced solutions in the framework of supervisory and control systems, increasing energy efficiency and reducing operating costs in the Urgent Center. The aim of this paper is the assessment of additional possibilities for increasing energy efficiency in the building using a number of control techniques available today. According to a source of energy, the most utilized is the electric power, which is consumed for air conditioning, compressed air, and lighting. Thermal energy is used for space heating in winter and the preparation of hot water. The first step in increasing energy efficiency was the continuous monitoring and recording of consumption. The next step was the analysis of the energy consumption and the discovery of critical areas of consumption. The final step was related to the plans and algorithms for the energy reduction. To this goal, the energy consumption in the period March 2014-February 2016 was measured and recorded. According to that measuring and data analysis, an expert system based on the methods of computational intelligence that combines all the developed actions and algorithms for increasing energy efficiency in one unit was implemented.

scholarly journals Causative agents of neonatal nosocomial infections and their resistance to antibiotics

2006 ◽  
Vol 59 (3-4) ◽  
pp. 155-159 ◽  
Author(s):  
Ljiljana Markovic-Denic ◽  
Jasna Djurisic ◽  
Tatjana Nikolic ◽  
Ruzdi Ramadani ◽  
Slobodanka Ilic ◽  
...  
Keyword(s):  
Nosocomial Infections ◽  
Neonatal Intensive Care ◽  
Environmental Control ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Coagulase Negative Staphylococci ◽  
Disk Diffusion Method ◽  
Gram Positive Bacteria ◽  
Causative Agents ◽  
Acinetobacter Spp

Introduction. The aim of the present study was to determine the most frequent microorganisms in a neonatal intensive care unit (NICU). Material and methods. A 6-month prospective study was conducted in a NICU. All neonatal hospital infections were registered, and microorganisms were isolated by standard methods. Their susceptibility to antibiotics was tested using the disk diffusion method. Results. One hundred and fifty-four neonatal nosocomial infections were detected. 87% of all infections were supported by a microbiological diagnosis, and 144 pathogens were isolated. Gram-negative bacteria were dominant (80%). The most commonly isolated microorganisms were Acinetobacter spp. (47.9%), Pseudomonas spp, (23.6%), Klebsiella/Enterobacter spp. (8.3%). Coagulase-negative staphylococci (8.3%) and Staphylococcus aureus (6.3%) were the most frequent reported gram-positive bacteria. All microorganisms showed resistance to most of commonly used antibiotics. Conclusion. Environmental control around neonatal patients and strict antibiotic policy are important in prevention of nosocomial transmission of resistant bacteria in the NICUs. .

Molecular Typing of Imipenem-ResistantAcinetobacter baumannii-calcoaceticusComplex in a Singapore Hospital Where Carbapenem Resistance Is Endemic

2007 ◽  
Vol 28 (8) ◽  
pp. 941-944 ◽  
Author(s):  
Thean Yen Tan ◽  
Karen Poh ◽  
Siew Yong Ng
Keyword(s):  
Tertiary Care ◽  
Carbapenem Resistance ◽  
Diffusion Method ◽  
Clinical Samples ◽  
Care Hospital ◽  
Typing Method ◽  
Disk Diffusion Method ◽  
Carbapenem Resistant ◽  
High Prevalence ◽  
Resistant Strains

Objective.To investigate the molecular epidemiology of carbapenem-resistantAcinetobacter baumannii-calcoaceticuscomplex isolates in a tertiary care hospital where the prevalence of carbapenem resistance among these organisms is high.Design.The study was a prospective, observational study performed during an 8-month period (May 1 through December 31, 2004).A. baumanniiisolates recovered from all clinical samples during the study period were included in the study. Antibiotic susceptibility testing was performed using the disk diffusion method, and all carbapenem-resistant strains were typed by a polymerase chain reaction-based typing method.Setting.An 800-bed hospital in Singapore.Results.More than half of recovered isolates were clonally unrelated, with the remaining isolates grouped into 4 genotypes.Conclusions.The results of the study suggest that the high prevalence of carbapenem resistance amongAcinetobacterorganisms in this institution is not caused by the spread of a predominant clone and that other factors may need to be investigated.

scholarly journals Mechanism of Klebsiella pneumoniae resistance to carbapenem antibiotics

2021 ◽  
Vol 18 (7) ◽  
pp. 1519-1526
Author(s):  
Danxia Chen ◽  
Yan Zhu ◽  
Liangshan Hu ◽  
Xiaoyun Wu ◽  
Mingyou Li ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Ethylene Diamine Tetraacetic Acid ◽  
Mechanism Of Action ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Tetraacetic Acid ◽  
Disk Diffusion Method ◽  
Beta Lactamases ◽  
Carbapenem Resistant ◽  
Resistant Strains

Purpose: To investigate the mechanism of action of Klebsiella pneumoniae (Kpn) resistance to carbapenem. Methods: The susceptibility of six Klebsiella pneumoniae strains to antibiotics was determined using KB assay. Six isolated strains which were resistant to carbapenem were identified and collected using modified Hodge test. The phenotypes of metal enzyme were evaluated by ethylene diamine tetraacetic acid (EDTA) disk diffusion method. The genes for beta-lactamases, including KPC gene, were examined. Results: The six carbapenem-resistant strains of Klebsiella pneumoniae were resistant to imipenem, meropenem and aztreonam, but were sensitive to amikacin, fosfomycin, minocycline, and polymyxin. The six pathogens did not produce metal enzyme, but they produced carbapenemases. Moreover, the six strains partially carried blaTEM or blaSHV gene, but all had blaKPC-2 gene. Conclusion: These results suggest that the pathogens that contain blaKPC-2 gene may be involved in the mechanism of Klebsiella pneumoniae (Kpn) resistance to carbapenem.

scholarly journals Prevalencia de especies zoonóticas de Arcobacter en cerdos a nivel de matadero en Ecuador

2020 ◽  
pp. e1798
Author(s):  
Rosa Janneth Simaluiza-Masabanda ◽  
Jimmy Fernández-Cajamarca ◽  
Heriberto Fernandez
Keyword(s):  
Antimicrobial Resistance ◽  
Multiplex Pcr ◽  
High Resistance ◽  
Probabilistic Method ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Fecal Carriage ◽  
Microbiological Methods ◽  
Southern Ecuador ◽  
Resistant Strains

Objective. To establish the prevalence, diversity and antimicrobial resistance of the zoonotic species of Arcobacter in healthy pigs at slaughterhouse level. Material and Methods. Fifty fecal samples were taken by rectal swabs from healthy pigs, before the beginning of the slaughter at the slaughterhouse of Loja city, Southern Ecuador. Sampling was performed using a non-probabilistic method for convenience. Isolation of Arcobacter strains was done by microbiological methods and species identification using biochemical and molecular (multiplex PCR) tests. Antimicrobial behavior was performed using the disk diffusion method. Results. The four zoonotic species of Arcobacter were found. The isolation rates were A. thereius (18.0%), A. skirrowii (18.0%), A. cryaerophilus (6.0%) and A. butzleri (2.0%). High resistance to ciprofloxacin was found and multi-resistant strains were isolated from these four species. Conclusions. The fecal carriage of the zoonotic species of Arcobacter was demonstrated in pigs at slaughterhouse level. These species showed high resistance to ciprofloxacin being isolated muti-resistant strains among these four species.

scholarly journals Daya Hambat Ekstrak Etanolik Buah Sirih Hijau (Piper betle L.) Terhadap Pertumbuhan Jamur Candida albicans (ATCC 1805)

2018 ◽  
Vol 5 (2) ◽  
pp. 77-83
Author(s):  
Ester Rampa
Keyword(s):  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Piper Betle ◽  
Fruit Extract ◽  
Disk Diffusion Method ◽  
Randomized Design ◽  
Fruit Samples ◽  
The University ◽  
And Control

The research had been conducted in the Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, the University of Cenderawasih during September–November 2012. The aims of the research were to understand the effect of green betel fruit extract inhibition against the C. albicans, as well as the effective concentration of extract to inhibit the growth of the fungus. Fruit samples were obtained from the Koya Koso village, Abepura District, Jayapura city, while the fungal isolates of C. albicans was obtained from the Laboratory of Microbiology, Faculty of Medicine, University of Padjadjaran. The study was performed using a method of disk diffusion method with concentration of 10%, 5%, 2.5% and control, while indicator parameter were measured based on the presence of inhibition zone caused by inhibition treatment. The study was designed on a Completely Randomized Design (CRD) and the data were analyzed using analysis of variance at α= 0.5 and α= 0.1. The presences of significant differences of data were further analyzed using Least Significance Differences (LSD) analyses. The results showed that all green betel fruit extract concentrations effectively inhibited the growth of the fungus C. albicans and concentration of 10% revealed the best inhibition effect.Key words: Inhibitory effect, Piper betle L, fruit extract, fungus. 

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