scholarly journals Significance of screening tests in diagnosis of infectious mononucleosis

2008 ◽  
Vol 61 (9-10) ◽  
pp. 489-496
Author(s):  
Gordana Kovacevic ◽  
Ivana Hrnjakovic-Cvjetkovic ◽  
Vesna Milosevic ◽  
Vera Jerant-Patic ◽  
Jelena Radovanov-Tadic ◽  
...  

The investigation included 91 patients in who an acute or previous EBV infection was established by ELISA test. All patients were also subjected to the Paul-Bunnell-Davidsohn test, while 20 patients were tested by the rapid screening test Clearview IM. The diagnosis of acute infective mononucleosis was in 61 patients (67%) confirmed by the Elisa test, and in 12 patients (19.67 %) by the Paul-Bunnell-Davidsohn test, while the rapid screening test Clearview IM demonstrated too low a detection of heterophile antibodies. The rapid screening test was not reliable. In 25% cases, the test was invalid, at early infection stages the rapid test failed to diagnose any case of the EBV virus infection. Paull-Bunell-Davidsohn was often negative, especially with young children. Therefore, priority should be given to virology tests based on the detection of specific antibodies to EBV antigen.

1970 ◽  
Vol 53 (6) ◽  
pp. 1224-1228
Author(s):  
Raymond J Sitkiewicz

Abstract A rapid screening test has been developed to determine killing dilutions of quaternary ammonium compounds. Two factors, Letheen broth as a quaternary ammonium compound neutralizer and triphenyltetrazolium chloride as a microbial growth indicator, resulted in a rapid test system for the evaluation of quaternary ammonium compounds. This rapid test system takes from 4 to 8 hr to complete as opposed to the 24 to 48 hr for other tests. A new germicide can be bacteriologically screened in one day.


2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Federica Romitelli ◽  
Leopoldo Paolo Pucillo ◽  
Umberto Basile ◽  
Enrico Di Stasio

Objectives. A new rapid, automatic, and sensitive screening test useful to detect cryoglobulins in serum samples is proposed.Design and Methods. The increase of turbidity during the cryoglobulin aggregation was monitored spectrophotometrically in sera from 400 patients with clinical evidence of cryoglobulinemia related disorders and 100 controls. Results were correlated to those obtained by the traditional method.Results. Kinetics of the aggregation curves were described by their maximum turbidity increase, lag time, and slope. Despite a partial correspondence between the traditional and the rapid test, patients with symptomatic cryoglobulinemia showed turbidity values significantly higher than the determined cutoff. Moreover, a functional classification of cryoglobulins is proposed.Conclusions. Due to its high reproducibility, operator independence, low cost, and results obtained within 2 hours, the rapid test can be used as a “real time” monitoring of cryoglobulinemia related diseases and for the evaluation of plasmapheresis efficacy.


1970 ◽  
Vol 102 (2) ◽  
pp. 237-237
Author(s):  
R. M. McDonald

Nature ◽  
1962 ◽  
Vol 193 (4822) ◽  
pp. 1298-1298 ◽  
Author(s):  
H. V. STREET

1993 ◽  
Vol 30 (3) ◽  
pp. 163-167 ◽  
Author(s):  
Pollen K.F. Yeung ◽  
Susan J. Mosher ◽  
Rongshi Li ◽  
Patrick S. Farmer ◽  
Gerald A. Klassen ◽  
...  

1971 ◽  
Vol 17 (11) ◽  
pp. 1081-1082 ◽  
Author(s):  
Jean E Matusik ◽  
James B Powell ◽  
David M Gregory

Abstract We have devised a rapid screening test for use in detecting sickling hemoglobins. This test depends on the insolubility of sickling hemoglobins in 2.24 molar phosphate buffer. Sickling hemoglobins form a turbid suspension in this test, in which positive and negative results, respectively, appear very much like those for the "Sickledex" test.


2019 ◽  
Vol 6 (1) ◽  
pp. e000329 ◽  
Author(s):  
Luc Durel ◽  
Guglielmo Gallina ◽  
Terence Pellet

Ceftiofur, a third-generation cephalosporin, is one of the most used antibiotics in dairy industry. Intramuscular injection of 1 mg/kgBW ceftiofur hydrochloride (HCl) generally results in 0 hour withdrawal time for the milk in dairy cows. Nevertheless, farmers and dairy processors occasionally complain about ceftiofur-based products in case of positive result to a commercial rapid screening test for the presence of violative residues of antimicrobials (inhibitors) in the bulk milk tank. Six lactating cows were injected with a 50 mg/ml ceftiofur HCl-based product at the dosage regimen of 1 mg/kg, intramuscularly, once a day, for five consecutive days, as per label. Milk samples were then collected just before the very last injection (T0) and then at 12, 24, 36, 48, 60, 72, 84 and 96 hours after the last injection. Individual milk samples were tested using three commercial screening test kits for inhibitor residues: DelvotestSP NT, SNAP Beta-Lactam ST Plus and ROSA MRL Beta-Lactam Test. Since bulk tank is screened in real operating conditions, samples were also diluted to 1:4, 1:10 and tested again. For the Delvotest SP NT, which lowest detected concentration is close the MRL of the ceftiofur (100 µg/kg), all results were negative. For the ROSA MRL Beta-Lactam Test and the SNAP Beta-Lactam ST Plus, several samples yielded positive and doubtful results at T0 and T12. However, after dilution to 1:10, all results were negative. Consequently, when used as officially instructed, the tested 50 mg/ml ceftiofur HCl-based injectable veterinary products are safe, and milk should be free of violative residues of ceftiofur. With consideration to the low specificity and the low positive predictive value of commercial screening tests, positive reactions of the bulk milk should be interpreted as false positive or another risky usage of β-lactam-based medicines in the farm must be investigated.


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