scholarly journals Effect of fermentation conditions on lipase production by Candida utilis

2007 ◽  
Vol 72 (8-9) ◽  
pp. 757-765 ◽  
Author(s):  
Sanja Grbavcic ◽  
Suzana Dimitrijevic-Brankovic ◽  
Dejan Bezbradica ◽  
Slavica Siler-Marinkovic ◽  
Zorica Knezevic
Keyword(s):  
Candida Utilis ◽  
Lipolytic Activity ◽  
Nitrogen Sources ◽  
Tween 80 ◽  
Lipase Production ◽  
Malt Extract ◽  
Fermentation Conditions ◽  
Carbon And Nitrogen ◽  
Wild Yeast ◽  
Negative Effect

A wild yeast strain isolated from spoiled soybean oil and identified as Candida utilis initially presented rather low lipase activity (approximately 4 IU dm-3) in submerged culture in a universal yeast medium containing 2 % malt extract. Studies were undertaken to improve the lipase production. The best yields of lipase were obtained with a medium supplemented with caprylic and oleic acids as inducers, but higher concentrations of the former (> 0.5 %) had a negative effect on the lipase production and cell growth. The type of nitrogen source seemed also to be very important. The highest lipolytic activity of 284 IU dm-3 was achieved after 5 days of fermentation in a medium containing oleic acid and hydrolyzed casein as carbon and nitrogen sources, respectively, and supplemented with Tween 80?. It was shown that optimization of the fermentation conditions can lead to a significant improvement in the lipase production (more than 70-fold higher compared to the initial value obtained in the non-optimized medium). .

scholarly journals DETERMINATION OF OPTIMUM CULTURE CONDITIONS FOR MYCELIAL GROWTH OF Macrolepiota procera MUSHROOM

2020 ◽  
Vol 19 (1) ◽  
pp. 11-20
Author(s):  
Aysun Pekşen ◽  
Beyhan Kibar
Keyword(s):  
Yeast Extract ◽  
Mycelial Growth ◽  
Nitrogen Sources ◽  
Culture Conditions ◽  
Malt Extract ◽  
The Public ◽  
Carbon And Nitrogen ◽  
Ph Values ◽  
Incubation Temperatures ◽  
Macrolepiota Procera

Macrolepiota procera, commonly called the Parasol Mushroom, is a delicious mushroom collected from the nature and commonly consumed by the public in many regions of Turkey. This study was conducted to determine the optimum culture conditions (pH, temperature, carbon and nitrogen sources) for mycelial growth of M. procera. Three pH values (pH 5.0, 5.5 and 6.0), four incubation temperatures (15, 20, 25 and 30°C), seven carbon (C) sources (dextrose, glucose, lactose, maltose, mannitol, sucrose and xylose) and six nitrogen (N) sources ((NH4)2HPO4, NH4NO3 and Ca(NO3)2, malt extract, peptone and yeast extract) were investigated. In the second step of the study, the effect of seven pH values (4.0, 4.5, 5.0, 5.5, 6.0, 6.5 and 7.0) on the mycelial colony diameter was examined at 20 and 25°C since these temperatures gave the best mycelial growth in the previously conducted temperature experiment. The best mycelial growth was determined at pH 6.0. The optimum temperature for mycelial growth of M. procera was found as 25°C. The use of glucose as carbon source and yeast extract and peptone as nitrogen source in the culture medium gave the best results for mycelial growth. Determining of optimum culture conditions for mycelial growth of M. procera will provide important contributions to the fortcoming studies on it’s commercially cultivation in Turkey.

scholarly journals Nitrogen source and pH interact and modulate lipase secretion in a non-clinical strain of Candida parapsilosis

2019 ◽  
Vol 41 ◽  
pp. e45481
Author(s):  
Rodolfo Krüger da Câmara Ribas ◽  
Diórgenes dos Santos Carboni ◽  
Juciana Clarice Cazarolli ◽  
Simone Hickmann Flôres ◽  
Maurício Ramirez-Castrillon ◽  
...  
Keyword(s):  
Nitrogen Source ◽  
Lipolytic Activity ◽  
Nitrogen Sources ◽  
Lipase Production ◽  
Industrial Applications ◽  
Enzyme Secretion ◽  
Clinical Strain ◽  
Surface Model ◽  
Yeast Strains ◽  
Initial Ph

Lipases (E.C. 3.1.1.3) are serine-hydrolases, and act on long chain fatty acid ester bonds. They exhibit specific and enantioselective activities, which are desirable for many industrial applications. This study aimed at screening and optimizing the production of lipases by wild yeast strains from a variety of substrates, as well as characterizing the enzyme. An initial selection was made in oxygenated oil-supplemented minimum medium, and the enzymatic activity of the supernatant was tested over p- nitrophenyl palmitate. One-hundred and twenty-four yeast strains from different substrates were tested, and twenty-three showed significantly higher lipolytic activity (p <0.01). One yeast in particular, QU110, showed best lipase production and therefore was selected for the optimization and characterization processes. This yeast exhibits enzyme secretion in initial pH 6.0, with olive oil and tryptone as carbon and nitrogen sources, respectively. There was a strong interaction between nitrogen source and initial pH, and pH 9.0 seems to inhibit enzyme secretion. The crude enzyme (cell-free supernatant) shows stability in surfactants and n-hexane, but not in ethanol or methanol. A Response Surface Model was created and optimal enzyme activity conditions were observed at 36°C and pH 8.0. The lipase is appropriate for transesterification reactions, as the enzyme is more stable in strong apolar solvents than moderately apolar ones. Also, secretion by pH was not reported elsewhere, which should be further investigated and contribute for other yeast bioprocesses as well.

Influence of carbon and nitrogen sources on lipase production by a newly isolated Candida viswanathii strain

2012 ◽  
Vol 63 (4) ◽  
pp. 1225-1234 ◽  
Author(s):  
Alex Fernando de Almeida ◽  
Sâmia Maria Taulk-Tornisielo ◽  
Eleonora Cano Carmona
Keyword(s):  
Nitrogen Sources ◽  
Lipase Production ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Candida Viswanathii

scholarly journals OPTIMIZATION OF ASPERGILLUS ORYZAE FERMENTATION CONDITIONS FOR KOJIC ACID PRODUCTION

2018 ◽  
Vol 10 (1) ◽  
pp. 316 ◽  
Author(s):  
Adnina Fitra Azzahra ◽  
Dyah Karina Puspita Sukarna ◽  
Herman Suryadi
Keyword(s):  
Aspergillus Oryzae ◽  
Yeast Extract ◽  
Acid Production ◽  
Kojic Acid ◽  
Nitrogen Sources ◽  
Carbon And Nitrogen Sources ◽  
Fermentation Conditions ◽  
Carbon And Nitrogen ◽  
Aeration Condition ◽  
Kojic Acid Production

Objective: The objective of this study is to obtain the optimal fermentation conditions for Aspergillus oryzae by gradually optimizing the medium andfermentation conditions.Methods: Fermentation condition were optimized by varying carbon and nitrogen sources, pH medium, temperature, and aeration condition. Levelsof kojic acid were determined using thin-layer chromatography-densitometry with an ultraviolet detector at a wavelength of 318 nm.Results: A combination of sucrose and yeast extract was chosen as the best source of carbon and nitrogen, respectively, from nine medium variations,producing 1.5425 g/L kojic acid. The optimum acidity of the medium was at pH 4.5, producing 1.7127 g/L of kojic acid, compared with pH 3.5 and5.5. Compared with fermentation at room temperature, fermentation at 35°C yielded greater kojic acid production. The best aeration condition was100 mL medium in 250 mL flask that produced the most kojic acid (1.6472 g/L).Conclusion: The optimal medium for fermentation of kojic acid using A. oryzae uses sucrose and yeast extract as carbon and nitrogen sources,respectively, at pH 4.5. The optimal aeration and incubation conditions were used, a volume of 100 mL in a 250 mL conical flask, incubated at 35°C,obtaining the highest yield value of 0.0370 g/g.

scholarly journals The preliminary optimization of culture components for improving antifungal production by a wild-type soil bacteria Bacillus subtilis

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 955-961
Author(s):  
Ramya Ramachandran ◽  
Kanika Bhatnagar ◽  
Ramlal Thakur ◽  
Utpal Roy
Keyword(s):  
Nitrogen Sources ◽  
Growth Conditions ◽  
Indicator Strain ◽  
Coefficient Of Determination ◽  
Bacillus Species ◽  
Antifungal Compound ◽  
Malt Extract ◽  
Antifungal Compounds ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen

Antifungal compounds are produced by Bacillus species under various growth conditions. While optimizing the antifungal production by using the one-factor-at-a-time (OFAT) and response surface methodology (RSM) approaches mannose and malt extract were identified as the most suitable carbon and nitrogen sources respectively. The RSM was applied to determine the optimum conditions of the three parameters such as pH, carbon and nitrogen sources for improved production. Optimum concentrations of carbon and nitrogen sources were 0.3% and 0.6% respectively with optimum media pH of 7.0 which showed optimum value of 40 AU/ml of antifungal compound against the Candida albicans SC5314 used as an indicator strain. In the present study, the F-value was determined as 0.0034 to imply that the model is significant. The goodness of the fit of the model was tested using coefficient of determination, R2  value, that tantamounts to 0.8562. The identification of antifungal compounds with their molecular masses was accomplished by MALDI-TOF mass spectrometry after n- butanol extraction. The present study thus has provided a platform to upgrade the yield of antifungal compounds which have got immense clinical potential to tread Candidosis.

scholarly journals Metabolism of Chicken Feathers and Concomitant Electricity Generation by Pseudomonas aeruginosa by Employing Microbial Fuel Cell (MFC)

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Venkatesh Chaturvedi ◽  
Pradeep Verma
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Fuel Cell ◽  
Microbial Fuel Cell ◽  
Nitrogen Sources ◽  
Electricity Production ◽  
Malt Extract ◽  
Feather Degradation ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Chicken Feathers

Keratinolytic potential of Pseudomonas aeruginosa strain SDS3 has been evaluated for the metabolism of chicken feathers. Results indicated that strain SDS3 showed complete metabolism of 0.1 and 0.5% (w/v) chicken feathers in minimal medium. Feathers were metabolized up to 80% at 1% (w/v) concentration. Maximum soluble protein (480.8±17.1 μg/mL) and keratinase (15.4±0.25 U/mL) were observed in the presence of 1% chicken feathers after five days of incubation. The effect of carbon and nitrogen sources showed that feather degradation was stimulated by complex carbon/nitrogen sources such as starch, malt extract, tryptone, and beef extract and was inhibited by simple carbon and nitrogen sources. Electricity production by employing chicken feathers as a substrate in microbial fuel cell (MFC) was evaluated. It was observed that maximum voltage corresponding to 141 mV was observed after 14 days of incubation. Maximum power density of 1206.78 mW/m2 and maximum current density of 8.6 mA/m2 were observed. The results clearly indicate that chicken feathers can be successfully employed as a cheap substrate for electricity production in MFC. This is the first report showing employment of chicken feathers as substrate in MFC.

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