scholarly journals Metabolism of Chicken Feathers and Concomitant Electricity Generation by Pseudomonas aeruginosa by Employing Microbial Fuel Cell (MFC)

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Venkatesh Chaturvedi ◽  
Pradeep Verma
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Fuel Cell ◽  
Microbial Fuel Cell ◽  
Nitrogen Sources ◽  
Electricity Production ◽  
Malt Extract ◽  
Feather Degradation ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Chicken Feathers

Keratinolytic potential of Pseudomonas aeruginosa strain SDS3 has been evaluated for the metabolism of chicken feathers. Results indicated that strain SDS3 showed complete metabolism of 0.1 and 0.5% (w/v) chicken feathers in minimal medium. Feathers were metabolized up to 80% at 1% (w/v) concentration. Maximum soluble protein (480.8±17.1 μg/mL) and keratinase (15.4±0.25 U/mL) were observed in the presence of 1% chicken feathers after five days of incubation. The effect of carbon and nitrogen sources showed that feather degradation was stimulated by complex carbon/nitrogen sources such as starch, malt extract, tryptone, and beef extract and was inhibited by simple carbon and nitrogen sources. Electricity production by employing chicken feathers as a substrate in microbial fuel cell (MFC) was evaluated. It was observed that maximum voltage corresponding to 141 mV was observed after 14 days of incubation. Maximum power density of 1206.78 mW/m2 and maximum current density of 8.6 mA/m2 were observed. The results clearly indicate that chicken feathers can be successfully employed as a cheap substrate for electricity production in MFC. This is the first report showing employment of chicken feathers as substrate in MFC.

scholarly journals Glycine Betaine Transmethylase Mutant of Pseudomonas aeruginosa

2002 ◽  
Vol 184 (15) ◽  
pp. 4301-4303 ◽  
Author(s):  
Ana L. Serra ◽  
Javier F. Mariscotti ◽  
José L. Barra ◽  
Gloria I. Lucchesi ◽  
Carlos E. Domenech ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Sequence Analysis ◽  
Glycine Betaine ◽  
Nitrogen Sources ◽  
Open Reading Frame ◽  
Carbon And Nitrogen Sources ◽  
Reading Frame ◽  
Carbon And Nitrogen ◽  
Molecular Approaches ◽  
Knockout Gene

ABSTRACT The gene for glycine betaine transmethylase (gbt) was identified in Pseudomonas aeruginosa strain Fildes III by biochemical, physiological, and molecular approaches. Based on sequence analysis, the knockout gene corresponded to an open reading frame (ORF) named PA3082 in the genome of P. aeruginosa PAO1. The translated product of this ORF displayed similarity to transferases of different microorganisms. Mutation in gbt blocked the utilization of choline and glycine betaine as carbon and nitrogen sources.

scholarly journals The preliminary optimization of culture components for improving antifungal production by a wild-type soil bacteria Bacillus subtilis

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 955-961
Author(s):  
Ramya Ramachandran ◽  
Kanika Bhatnagar ◽  
Ramlal Thakur ◽  
Utpal Roy
Keyword(s):  
Nitrogen Sources ◽  
Growth Conditions ◽  
Indicator Strain ◽  
Coefficient Of Determination ◽  
Bacillus Species ◽  
Antifungal Compound ◽  
Malt Extract ◽  
Antifungal Compounds ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen

Antifungal compounds are produced by Bacillus species under various growth conditions. While optimizing the antifungal production by using the one-factor-at-a-time (OFAT) and response surface methodology (RSM) approaches mannose and malt extract were identified as the most suitable carbon and nitrogen sources respectively. The RSM was applied to determine the optimum conditions of the three parameters such as pH, carbon and nitrogen sources for improved production. Optimum concentrations of carbon and nitrogen sources were 0.3% and 0.6% respectively with optimum media pH of 7.0 which showed optimum value of 40 AU/ml of antifungal compound against the Candida albicans SC5314 used as an indicator strain. In the present study, the F-value was determined as 0.0034 to imply that the model is significant. The goodness of the fit of the model was tested using coefficient of determination, R2  value, that tantamounts to 0.8562. The identification of antifungal compounds with their molecular masses was accomplished by MALDI-TOF mass spectrometry after n- butanol extraction. The present study thus has provided a platform to upgrade the yield of antifungal compounds which have got immense clinical potential to tread Candidosis.

scholarly journals Effects of carbon and nitrogen sources on the proteome of Pseudomonas aeruginosa PA1 during rhamnolipid production

2010 ◽  
Vol 45 (9) ◽  
pp. 1504-1510 ◽  
Author(s):  
Rodrigo Siqueira Reis ◽  
Surza Lucia Gonçalves da Rocha ◽  
Donat Alexander Chapeaurouge ◽  
Gilberto Barbosa Domont ◽  
Lidia Maria Melo Santa Anna ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Nitrogen Sources ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Rhamnolipid Production

Evaluation of Different Carbon and Nitrogen Sources in Production of Rhamnolipids by a Strain of Pseudomonas aeruginosa

2002 ◽  
Vol 98-100 (1-9) ◽  
pp. 1025-1036 ◽  
Author(s):  
Alexandre S. Santos ◽  
Ana Paula W. Sampaio ◽  
Gina S. Vasquez ◽  
Lídia M. Santa Anna ◽  
Nei Pereira Jr. ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Nitrogen Sources ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen

Evaluation of Different Carbon and Nitrogen Sources in Production of Rhamnolipids by a Strain of Pseudomonas aeruginosa

2002 ◽  
pp. 1025-1035 ◽  
Author(s):  
Alexandre S. Santos ◽  
Ana Paula W. Sampaio ◽  
Gina S. Vasquez ◽  
Lídia M. Santa Anna ◽  
Nei Pereira ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Nitrogen Sources ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen

Electricity Production from Dual Chambers Microbial Fuel Cell Fed with Chicken Manure-Wastewater

2015 ◽  
Vol 3 (1) ◽  
pp. 9-18
Author(s):  
Ali J. Jaeel
Keyword(s):  
Fuel Cell ◽  
Microbial Fuel Cell ◽  
Chicken Manure ◽  
Electricity Production ◽  
Anaerobic Sludge ◽  
Livestock Wastewater ◽  
Graphite Electrodes ◽  
Reliable Source ◽  
Current Densities ◽  
Resistance Value

Chicken manure wastewaters are increasingly being considered a valuable resource of organic compounds. Screened chicken manure was evaluated as a representative solid organic waste. In this study, electricity generation from livestock wastewater (chicken manure) was investigated in a continuous mediator-less horizontal flow microbial fuel cell with graphite electrodes and a selective type of membrane separating the anodic and cathodic compartments of MFC from each other. The performance of MFC was evaluated to livestock wastewater using aged anaerobic sludge. Results revealed that COD and BOD removal efficiencies were up to 88% and 82%, respectively. At an external resistance value of 150 Ω, a maximum power and current densities of 278 m.W/m2 and 683 mA/m2, respectively were obtained, hence MFC utilizing livestock wastewater would be a sustainable and reliable source of bio-energy generation .

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