scholarly journals Extraction of flavonoids from garden (Salvia officinalis L.) and glutinous (Salvia glutinosa L.) sage by ultrasonic and classical maceration

2007 ◽  
Vol 72 (1) ◽  
pp. 73-80 ◽  
Author(s):  
Dragan Velickovic ◽  
Milena Nikolova ◽  
Stephanie Ivancheva ◽  
Jelena Stojanovic ◽  
Vlada Veljkovic

Flavonoids were analyzed in the extracts of garden (Salvia officinalis L.) and glutinous (Salvia glutinosa L.) sage. Ultrasonic extraction (20 minutes at 40?C) and classical maceration (6 h at room temperature) of the extractable substances from dried herbs and dried residual plant materials from which the essential oil had previously been removed by hydrodistillation were performed with petroleum ether, 70 % aqueous solution of ethanol and water. It was found that the extracts from both plants contained flavonoids, but their compositions were dependent of the plant species, the polarity of the extracting solvent and the extraction technique applied. Apigenin and its derivatives (e.g., apigenin 4'-methyl ether), scutellarein 6-methyl ether, isoscutellarein 8-methyl ether, luteolin and 6-OH-luteolin-6-methyl ether where distinctive for S. officinalis. Apigenin, luteolin, 6-OH-luteolin-6-methyl ether, kaempherol 3-methyl ether, kaempherol 3,7-dimethyl ether, quercetin 3,7,3'-trimethyl ether and quercetin 3,7,3',4'-tetramethyl ether were distinctive for S. glutinosa. The flavonoids were also detected in considerable quantities in the plant material from which the essential oils had been already removed. Hence, this industrial waste plant material might be further used as a source of the flavonoids. .

2011 ◽  
Vol 65 (5) ◽  
pp. 599-605 ◽  
Author(s):  
Dragan Velickovic ◽  
Ivana Karabegovic ◽  
Sasa Stojicevic ◽  
Miodrag Lazic ◽  
Valentina Marinkovic ◽  
...  

Antioxidant and antimicrobial activities as well as total phenols and flavonoids contents of Salvia glutinosa L. (glutinous sage) and Salvia officinalis L. (sage) extracts were studied. Methanol and aqueous ethanol (70% v/v) were used for extraction of bioactive compounds, both in the presence and the absence of ultrasound, from herb and the spent plant material remaining after the essential oil hydrodistillation. The ratio of plant material to extracting solvent was 1:10 g/ml. Antioxidant and antimicrobial activities of the extracts were found to depend on the type of plant material and the extraction conditions. The plant materials from which essential oil had been recovered were proven to be valuable raw materials for making various herbal preparations.


2019 ◽  
Vol 70 (6) ◽  
pp. 2015-2020
Author(s):  
Silvia Robu ◽  
Aurelia Romila ◽  
Olimpia Dumitriu Buzia ◽  
Adrian Florin Spac ◽  
Camelia Diaconu ◽  
...  

Numerous articles on Salvia officinalis L. have been published regarding the composition of their essential oil. The considerable variation found may be due to the quality of the plant material as well as to the methods used for analysis. A simple GC-MS method was developed and optimized in the QbD approach, for the determination of sage essential oils. The optimization of GC-MS analysis was performed using different mobile phase flows, injection volumes, split ratios and temperature programs. The optimized method proved to be simple and can be successfully applied for the determination of sage essential oils.


1992 ◽  
Vol 22 (5) ◽  
pp. 585-590 ◽  
Author(s):  
Z. Djarmati ◽  
R. M. Jankov ◽  
J. Csanadi ◽  
A. Djordjević ◽  
B. Ribar ◽  
...  

2008 ◽  
Vol 3 (10) ◽  
pp. 1934578X0800301 ◽  
Author(s):  
Necdet Çamaş ◽  
Jolita Radušienė ◽  
Ali Kemal Ayan ◽  
Cüneyt Çırak ◽  
Valdimaras Janulis ◽  
...  

The present study was conducted to determine the variation in the content of hyperforin, hypericin and pseudohypericin in Hypericum triquetrifolium Turra growing wild in four locations of Turkey. The aerial parts, representing a total of 30 individuals, were collected at full flowering and dissected into floral, leaf and stem tissues. After drying at room temperature, the plant materials were assayed for their chemical contents by HPLC. The populations varied significantly in chemical contents. Hyperforin content ranged from 0.05 to 0.56 mg/g, hypericin from 0.74–1.98 mg/g, and pseudohypericin from 0.72–2.26 mg/g, dry weight. Among the different plant parts, the flowers were found to be the principle organ for hyperforin accumulation, while hypericin and pseudohypericin were accumulated mainly in leaves. Such kinds of data could be useful for optimizing the processing methodology of wild-harvested plant material and phytochemical evaluation of H. triquetrifolium.


2021 ◽  
Vol 40 ◽  
pp. 03004
Author(s):  
Anastasia Moldavanova ◽  
Anastasia Malyutina ◽  
Larissa Kozubova ◽  
Valentina Kazakova ◽  
Natalia Shestopalova

Inflammatory diseases of the female genital area are dominant among the total number of gynecological diseases. Among all the variety of medicinal plant materials used in official medicine, salvia medicinal draws attention to itself, which has repeatedly proved its effectiveness in the treatment of inflammatory diseases of the mucous membranes of various origins. That is why the goal was set to develop a technology for obtaining a liquid extract from the leaves of Salvia officinalis L. As a result of the study, a method was developed for obtaining a liquid extract by selecting the optimal extraction conditions. It was found that the optimal raw material-extractant ratio is 1:50, the optimal concentration of the extractant ethyl alcohol is 70%, the infusion time of the extract is 24 hours. To intensify the extraction process, the method of ultrasonic processing of raw materials at the soaking stage was used. The optimal time for processing plant materials with ultrasound was 45 minutes. As a result of using this method to obtain a liquid extract from the leaves of sage officinalis, the yield of phenolic compounds was doubled in comparison with obtaining an extract without sonication of raw materials and amounted to 182.7 mg / g of flavonoids in terms of luteolin-7-glycoside.


1957 ◽  
Vol 37 (1) ◽  
pp. 1-11 ◽  
Author(s):  
M. W. Cormack ◽  
R. W. Peake ◽  
R. K. Downey

Several methods for inoculation of alfalfa with the bacterial wilt organism were compared in the greenhouse and field. The root-ball soak method, not previously described, proved particularly rapid and effective for greenhouse tests. All methods of root and crown inoculation tested gave good results, but spraying and other methods of stem inoculation were not dependable. In the field, the best results were obtained with bare-root soak inoculation before transplanting, and with hypodermic injection of the roots in the fall.Cultures of the bacterium of tested virulence and infected plant materials proved equally effective as inoculum, but the latter were more easily handled and stored. The organism remained highly viable in dried plant material stored for three years at room temperature, 5 °C, and −20 °C. The pulverized plant inoculum gave best results when soaked in water for 4 to 12 hours before use. It was gradually inactivated with longer periods of soak.Infection increased progressively with age in seedlings and rooted cuttings of Grimm alfalfa inoculated in the greenhouse at ages ranging from 3 to 10 weeks. Maximum susceptibility at 7 to 10 weeks of age was associated with the extensive formation of secondary tissues in the root and crown. The disease generally developed more rapidly in seedlings than in clonal material.


Author(s):  
Çiğdem Sönmez ◽  
Adem Gökçöl ◽  
Ayşe Özge Şimşek Soysal ◽  
Emine Bayram ◽  
Ahmet Esen Çelen

In this study, Common Sage (Salvia officinalis L.) and Anatolian Sage (Salvia fruticosa Mill.) seeds, which are important medicinal plants, were used as plant material. The seeds of the Salvia genus have dormancy as they have seed husks containing a mucilaginous layer. For this reason, in order to improve the germination and emergence performance of seeds and to increase the quality of seeds, nine pre-sowing treatments (KNO3 application, GA3 application, polymer coating, polymer coating + KNO3, polymer coating + GA3, priming, priming + polymer coating, priming + polymer coating + KNO3, priming + polymer coating + GA3) were applied to the seeds and the effect of applications on germination rate, emergency rate, germination speed and emergency speed were investigated.


Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
A Aprotosoaie ◽  
E Gille ◽  
A Spac ◽  
M Gonceariuc ◽  
M Hancianu ◽  
...  

2019 ◽  
Vol 25 (1) ◽  
Author(s):  
NARENDRA SINGH ◽  
N. S. BHADAURIA ◽  
PRADYUMN SINGH

The Bio-efficacy of eleven plant extracts namely viz.Neem Kernel; Rhizome of Ginger; Leaves of Datura, Gajarghas, Harsingar, Oak and Latjeera; Bulb of Garlic and Onion; Flowers of Chrysenthemum and Fruits of Chilli in the concentration of 5 percent and imidacloprid @ 40 g ai/ha was tested against mustard aphid, Lipaphiserysimi and their effect on D. rapae and Coccinellid beetle were tested in the Department of Entomology, College of Agriculture, Gwalior (M.P.). All the tested plant materials and imidacloprid @ 40 g ai/ha were effective significanty in reducing the aphid population over control.The aphid population in treated plots ranged from 7.2 to 40.0 as against 85.4 aphid/twig in untreated control. Among the plant material, three sprays of Neem Kernel were found most effective followed by three sprays of chilli fruits.All the plant extracts were found significantly safer to D. rapae and coccinellid bettle in comparision to insecticide (imidacloprid).


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