scholarly journals In vitro complexes of copper and zinc with chlorophyll

2006 ◽  
Vol 71 (5) ◽  
pp. 501-512 ◽  
Author(s):  
Jelena Petrovic ◽  
Goran Nikolic ◽  
Dejan Markovic
Keyword(s):  
Fluorescence Spectroscopy ◽  
Magnesium Atom ◽  
Chelate Cycle ◽  
Copper And Zinc

Complexes of copper and zinc with chlorophyll, the major photosynthesis pigment, were studied by Vis, FTIR and fluorescence spectroscopy. Two types of complexes were recognized. While copper replaces the central magnesium atom of chlorophyll to form a "central" Cu-Chl complex, this was not proposed in the case of zinc. Instead, the zinc-mediated formation of a 6-membered chelate cycle fused at the periphery of the chlorophyll structure is proposed. The latter event could be ascribed to allomerization reactions of chlorophyll.

Study of in vitro interaction between tetrabromobisphenol A and bovine serum albumin by fluorescence spectroscopy

2011 ◽  
Vol 30 (12) ◽  
pp. 2697-2700 ◽  
Author(s):  
Yingxin Wu ◽  
Yan Qian ◽  
Hao Cui ◽  
Xiaomin Lai ◽  
Xianchuan Xie ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Fluorescence Spectroscopy ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Tetrabromobisphenol A ◽  
In Vitro Interaction

In vitro Growth of Genovese Basil in Response to Different Concentrations of Salts and Interaction of Sucrose and Activated Carbon

2018 ◽  
Vol 10 (9) ◽  
pp. 142 ◽  
Author(s):  
J. R. Trettel ◽  
A. B. Nascimento ◽  
L. N. Barbosa ◽  
H. M. Magalhães
Keyword(s):  
Activated Carbon ◽  
Callus Formation ◽  
Sucrose Concentration ◽  
Ionic Balance ◽  
In Vitro Growth ◽  
Economic Potential ◽  
Toxic Compounds ◽  
Copper And Zinc ◽  
High Concentrations

Genovese basil has great economic potential; however, there is no established micropropagation protocol for this species. Therefore, this study aimed at assessing the in vitro growth of Genovese basil in response to different concentrations of salts in the Murashige and Skoog medium (MS) and interaction of sucrose and activated carbon. Two assays were conducted independently in an in vitro environment using the MS medium, regulators, agar, and five salt concentrations (0, 25, 50, 70 and 100%). In the second assay, two concentrations of sucrose (30 and 60 g L-1) and three concentrations of activated carbon (0, 3.0, and 4.5 g L-1) were tested.In addition, copper and zinc were quantified in the roots. The results showed that shoots were favored when the medium was at its full strength (100% salts), with seedlings forming more leaves.This result may be associated with a higher demand for nitrogen and because of the ionic balance between NH4+ and NO3-. High concentrations of salts affected the roots, but a reduction to 70% salt favored root development. Doubling the usual dose of sucrose (60 g L-1) damaged the growth of the seedlings. Damage caused by osmotic and oxidative potentials, and by toxic compounds may be related to the observed results. The amount of copper and zinc in the root increased with increased concentrations of activated carbon in the medium. The presence of activated carbon reduced callus formation but did not mitigate the effects of increased sucrose concentration.

Inhibition of glutathione and s-allyl glutathione on pancreatic lipase: Analysis through in vitro kinetics, fluorescence spectroscopy and in silico docking

2020 ◽  
Vol 160 ◽  
pp. 623-631
Author(s):  
Palvannan Thayumanavan ◽  
Selvan Nallaiyan ◽  
Chitra Loganathan ◽  
Penislusshiyan Sakayanathan ◽  
Saravanan Kandasamy ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
Pancreatic Lipase ◽  
In Silico ◽  
In Silico Docking ◽  
In Vitro Kinetics

Biosensing Surfaces Based on Quantum Dots Array

2014 ◽  
Vol 490-491 ◽  
pp. 1602-1606 ◽  
Author(s):  
Jana Drbohlavová ◽  
Jana Chomoucka ◽  
Radim Hrdý ◽  
Vojtech Svatos ◽  
Jaromir Hubalek
Keyword(s):  
Quantum Dots ◽  
Chemical Composition ◽  
Fluorescence Spectroscopy ◽  
Surface Topography ◽  
Electrochemical Detection ◽  
Physical Adsorption ◽  
Homogeneous Distribution ◽  
Alumina Template ◽  
Detection Of Biomolecules

The fabrication of self-ordered semiconductor (TiO2) and noble metal (Au) QDs arrays was successfully achieved by advanced nonlithographic template based method, namely using nanoporous alumina template. The emphasis was placed on the successful preparation of QDs arrays with the desired size, homogeneous distribution and optical (especially fluorescence) properties. Titania and gold QDs characterization by SEM, EDX and fluorescence spectroscopy was performed in order to verify their surface topography, chemical composition and emission properties in UV/VIS range of spectra, respectively. The surface biofunctionalization of QDs was realized via simple physical adsorption of glutathione tripeptide, which makes these arrays suitable for potential biosensing application, mainly in optical and electrochemical detection of biomoleculesin vitro.

Two-photon excited fluorescence spectroscopy and imaging of melanin in vitro and in vivo

2012 ◽  
Author(s):  
Tatiana B. Krasieva ◽  
Feng Liu ◽  
Chung-Ho Sun ◽  
Yu Kong ◽  
Mihaela Balu ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
Two Photon
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