Fluorescence spectroscopy and SHG imaging of an in vitro collagen model

2006 ◽  
Author(s):  
N.D. Kirkpatrick ◽  
U. Utzinger
Keyword(s):  
Fluorescence Spectroscopy

Study of in vitro interaction between tetrabromobisphenol A and bovine serum albumin by fluorescence spectroscopy

2011 ◽  
Vol 30 (12) ◽  
pp. 2697-2700 ◽  
Author(s):  
Yingxin Wu ◽  
Yan Qian ◽  
Hao Cui ◽  
Xiaomin Lai ◽  
Xianchuan Xie ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Fluorescence Spectroscopy ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Tetrabromobisphenol A ◽  
In Vitro Interaction

Inhibition of glutathione and s-allyl glutathione on pancreatic lipase: Analysis through in vitro kinetics, fluorescence spectroscopy and in silico docking

2020 ◽  
Vol 160 ◽  
pp. 623-631
Author(s):  
Palvannan Thayumanavan ◽  
Selvan Nallaiyan ◽  
Chitra Loganathan ◽  
Penislusshiyan Sakayanathan ◽  
Saravanan Kandasamy ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
Pancreatic Lipase ◽  
In Silico ◽  
In Silico Docking ◽  
In Vitro Kinetics

scholarly journals In vitro complexes of copper and zinc with chlorophyll

2006 ◽  
Vol 71 (5) ◽  
pp. 501-512 ◽  
Author(s):  
Jelena Petrovic ◽  
Goran Nikolic ◽  
Dejan Markovic
Keyword(s):  
Fluorescence Spectroscopy ◽  
Magnesium Atom ◽  
Chelate Cycle ◽  
Copper And Zinc

Complexes of copper and zinc with chlorophyll, the major photosynthesis pigment, were studied by Vis, FTIR and fluorescence spectroscopy. Two types of complexes were recognized. While copper replaces the central magnesium atom of chlorophyll to form a "central" Cu-Chl complex, this was not proposed in the case of zinc. Instead, the zinc-mediated formation of a 6-membered chelate cycle fused at the periphery of the chlorophyll structure is proposed. The latter event could be ascribed to allomerization reactions of chlorophyll.

Biosensing Surfaces Based on Quantum Dots Array

2014 ◽  
Vol 490-491 ◽  
pp. 1602-1606 ◽  
Author(s):  
Jana Drbohlavová ◽  
Jana Chomoucka ◽  
Radim Hrdý ◽  
Vojtech Svatos ◽  
Jaromir Hubalek
Keyword(s):  
Quantum Dots ◽  
Chemical Composition ◽  
Fluorescence Spectroscopy ◽  
Surface Topography ◽  
Electrochemical Detection ◽  
Physical Adsorption ◽  
Homogeneous Distribution ◽  
Alumina Template ◽  
Detection Of Biomolecules

The fabrication of self-ordered semiconductor (TiO2) and noble metal (Au) QDs arrays was successfully achieved by advanced nonlithographic template based method, namely using nanoporous alumina template. The emphasis was placed on the successful preparation of QDs arrays with the desired size, homogeneous distribution and optical (especially fluorescence) properties. Titania and gold QDs characterization by SEM, EDX and fluorescence spectroscopy was performed in order to verify their surface topography, chemical composition and emission properties in UV/VIS range of spectra, respectively. The surface biofunctionalization of QDs was realized via simple physical adsorption of glutathione tripeptide, which makes these arrays suitable for potential biosensing application, mainly in optical and electrochemical detection of biomoleculesin vitro.

Two-photon excited fluorescence spectroscopy and imaging of melanin in vitro and in vivo

2012 ◽  
Author(s):  
Tatiana B. Krasieva ◽  
Feng Liu ◽  
Chung-Ho Sun ◽  
Yu Kong ◽  
Mihaela Balu ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
Two Photon

Mitochondria-targeted porphyrin-based photosensitizers containing triphenylphosphonium cations showing efficient in vitro photodynamic therapy effects

2019 ◽  
Vol 23 (11n12) ◽  
pp. 1505-1514 ◽  
Author(s):  
Xing Guo ◽  
Hao Wu ◽  
Wei Miao ◽  
Yangchun Wu ◽  
Erhong Hao ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Cancer Therapy ◽  
Fluorescence Spectroscopy ◽  
Hela Cells ◽  
Photophysical Properties ◽  
Quantum Yields ◽  
Subcellular Organelle

Subcellular organelle-targeted photosensitizers have recently reported to be effective photodynamic therapy (PDT) agents. In this work, three porphyrin-derived photosensitizers, containing one, two or four triphenylphosphonium targeting groups, were synthesized and characterized by NMR, HRMS, UV-vis and fluorescence spectroscopy. These photosensitizers showed similar photophysical properties to classical porphyrins and exhibited excellent [Formula: see text]O[Formula: see text] quantum yields in acetonitrile. Subcellular colocalization indicated that all three photosensitizers specifically stain the mitochondria of HeLa cells. Photosensitizer mito-dp, containing two triphenylphosphonium cations was found to be the most uptaken by cells and exhibited the best PDT effect with an effective phototoxicity (IC[Formula: see text] (light) [Formula: see text] 12.4 nM), suggestive of a higher practicable potential of mitochondria-targeted PDT agents in cancer therapy.

scholarly journals Recombinant Coat Protein of Banana Bract Mosaic Virus as a Potential Antigen for Serological Detection of the Virus

2020 ◽  
pp. 47-60
Author(s):  
Darsana Dilip ◽  
Vimi Louis ◽  
Pallavi Sabharwal ◽  
H. S. Savithri ◽  
P. M. Namitha ◽  
...  
Keyword(s):  
Coat Protein ◽  
Fluorescence Spectroscopy ◽  
Mosaic Virus ◽  
Gene Segment ◽  
Mosaic Disease ◽  
Polyclonal Antiserum ◽  
Expression Vectors ◽  
Affinity Column ◽  
Banana Bract Mosaic Virus

Banana bract mosaic disease caused by Banana bract mosaic potyvirus (BBrMV) is reported to instigate heavy loss in banana and plantain across Asia. Almost all the cultivars of banana succumb to the disease resulting in malformed bunches weighing less than half of normal ones. In the current study the coat protein (CP) gene segment present at the 3’ terminal region of the viral genome amplified by RT-PCR was cloned into expression vectors, pRSET-C and pGEX-4T-2 to use it for raising polyclonal antiserum which in turn will aid in developing assays to detect the virus. Recombinant BBrMV CP (rCP) in pRSET-C when expressed was insoluble whereas, it was in the soluble fraction when expressed from pGEX-4T-2. The GST-fusion protein was purified by GSH sepharose affinity column chromatography and western blot analysis was performed using anti GST antibodies. 360 µg/ml of protein was purified from 1 l of culture. The GST tag was cleaved from the purified protein by incubation with thrombin at 25°C overnight.  The rCP was characterized using ultracentrifugation, fluorescence spectroscopy and electron microscopy. The tagless monomer failed to assemble to virus like particles (VLPs) in vitro which was substantiated by fluorescence spectroscopy. This study will be first step towards deciphering structure and functions of Banana bract mosaic virus coat protein.

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