scholarly journals Scanning probe microscopy characterization of immobilized enzyme molecules on a biosensor surface: Visualisation of individual molecules

2004 ◽  
Vol 69 (2) ◽  
pp. 93-106 ◽  
Author(s):  
Dusan Losic ◽  
Ken Short ◽  
Justin Gooding ◽  
Joe Shapter
Keyword(s):  
Scanning Probe Microscopy ◽  
Immobilized Enzyme ◽  
Scanning Probe ◽  
Probe Microscopy ◽  
Force Microscopy ◽  
Tunnelling Microscopy ◽  
Enzyme Molecules ◽  
Microscopy Characterization ◽  
Microscopy Techniques ◽  
Stm Images

Scanning probe microscopy techniques were used to study immobilized enzyme molecules of glucose oxidase (GOD) on a biosensor surface. The study was carried out in order to optimize atomic force microscopy (AFM) imaging and reveal themolecular resolution of individual GOD molecules. Chemically modified AFM tips and the light tapping mode were found to be the optimal conditions for imaging soft biomolecules such as GOD. The information obtained from the AFM images included spatial distribution and organization of the enzyme molecules on the surface, surface coverage and shape, size and orientation of individual molecules. Two typical shapes of GOD molecules were found, spherical and butterfly, which are in accordance with the shapes obtained from scanning tunnelling microscopy (STM) images. Using a model of the orientation of the GOD molecules on the surface, these shapes are assigned to the enzyme standing and lying on the surface. After AFM tip deconvolution, the size of the spherical shaped GOD molecules was found to be 12 ?2.1 nm in diameter, whereas the butterfly shapes were 16.5 ? 3.3 nm x10.2 ? 2.5 nm. Corresponding STM images showed smaller lateral dimensions of 10 _1nm_ 6 ?1nm and 6.5 ? 1 nm x5 ? 1 nm. The disagreement between these two techniques is attributed to the deformation of the GOD molecules caused by the tapping process.

Developments in Using Scanning Probe Microscopy To Study Molecules on Surfaces — From Thin Films and Single-Molecule Conductivity to Drug–Living Cell Interactions

2006 ◽  
Vol 59 (6) ◽  
pp. 359 ◽  
Author(s):  
Pall Thordarson ◽  
Rob Atkin ◽  
Wouter H. J. Kalle ◽  
Gregory G. Warr ◽  
Filip Braet
Keyword(s):  
Single Molecule ◽  
Scanning Probe Microscopy ◽  
Cell Interactions ◽  
Scanning Probe ◽  
Probe Microscopy ◽  
Force Microscopy ◽  
Atomic Force ◽  
Tunnelling Microscopy ◽  
Molecule Surface

Scanning probe microscopy (SPM) techniques, including atomic force microscopy (AFM) and scanning tunnelling microscopy (STM), have revolutionized our understanding of molecule–surface interactions. The high resolution and versatility of SPM techniques have helped elucidate the morphology of adsorbed surfactant layers, facilitated the study of electronically conductive single molecules and biomolecules connected to metal substrates, and allowed direct observation of real-time processes such as in situ DNA hybridization and drug–cell interactions. These examples illustrate the power that SPM possesses to study (bio)molecules on surfaces and will be discussed in depth in this review.

Reliability of Thin Thermally Grown SiO2 on 3C-SiC Studied by Scanning Probe Microscopy

2010 ◽  
Vol 645-648 ◽  
pp. 833-836 ◽  
Author(s):  
Jens Eriksson ◽  
Ming Hung Weng ◽  
Fabrizio Roccaforte ◽  
Filippo Giannazzo ◽  
Patrick Fiorenza ◽  
...  
Keyword(s):  
Scanning Probe Microscopy ◽  
Dielectric Breakdown ◽  
Sample Surface ◽  
Scanning Probe ◽  
Probe Microscopy ◽  
Force Microscopy ◽  
Local Current ◽  
Microscopy Techniques ◽  
Cubic Silicon Carbide ◽  
Thermally Grown

This paper reports on the electrical characteristics of thermally grown SiO2 on cubic silicon carbide (3C-SiC). The 3C-SiC (111) was grown on Si-face 6H-SiC (0001) on-axis substrates by a non-conventional Vapor-Liquid-Solid (VLS) technique. Scanning probe microscopy techniques, including Atomic Force Microscopy (AFM), Scanning Capacitance Microscopy (SCM) and tunneling AFM (TUNA) were employed to study the morphology, local capacitance and local current variations across the sample surface. This nanoscale investigation allowed monitoring the homogeneity, as well as reliability in terms of dielectric breakdown (BD), of the thermally grown SiO2. In this way it was possible to gain insights into the breakdown related to pre-existing defects (extrinsic breakdown) as well as the actual intrinsic breakdown of the dielectric.

2013 Microscopy Today Innovation Awards

2013 ◽  
Vol 21 (5) ◽  
pp. 40-45
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Scanning Probe Microscopy ◽  
Scanning Probe ◽  
Probe Microscopy ◽  
Analysis Methods ◽  
Microscopy Analysis ◽  
Ion Microscopy ◽  
Microscopy Techniques

Microscopy Today congratulates the fourth annual group of Innovation Award winners. The ten innovations described below move several microscopy techniques forward: light microscopy, scanning probe microscopy, electron microscopy, ion microscopy, and hybrid microscopy-analysis methods. These innovations will make imaging and analysis more powerful, more flexible, more productive, and easier to accomplish.

scholarly journals Origin of surface potential in undoped zinc oxide films revealed by advanced scanning probe microscopy techniques

RSC Advances ◽  
2017 ◽  
Vol 7 (67) ◽  
pp. 42393-42397
Author(s):  
Ting Su
Keyword(s):  
Zinc Oxide ◽  
Surface Potential ◽  
Scanning Probe Microscopy ◽  
Oxide Films ◽  
Scanning Probe ◽  
Zno Film ◽  
Probe Microscopy ◽  
Zinc Oxide Films ◽  
Combined Use ◽  
Microscopy Techniques

Surface potential of undoped ZnO film has been studied by a combined use of PFM and KPFM techniques.

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