scholarly journals Normal-phase high performance liquid chromatography of estradiol derivatives on amino- and diol-columns

2003 ◽  
Vol 68 (12) ◽  
pp. 971-977
Author(s):  
Marijana Acanski
Keyword(s):  
High Performance Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Volume Fraction ◽  
Stationary Phases ◽  
Normal Phase ◽  
Retention Behavior ◽  
Estradiol Derivatives ◽  
Binary Mobile Phase ◽  
The Relationship

The retention behavior of estradiol derivatives was studied by HPLC on chemically bonded polar stationary phases: commercially available amino and diol- columns, as a function of the heptane-propan-1-ol as the mobile phase, when the volume fraction of propan-1-ol in the binary mobile phase was low, even less than 5 %. The relationship between the logarithm of the retention constant (log k) and the logarithm of the volume fraction of propan-1-ol (?log ?) in the eluent was linear for all solutes studied. The results are discussed in terms of the solute and stationary phase properties and compared with the results of the same derivatives obtained in earlier investigations.

scholarly journals Comparison of Retention Behavior between Supercritical Fluid Chromatography and Normal-Phase High-Performance Liquid Chromatography with Various Stationary Phases

Molecules ◽  
2019 ◽  
Vol 24 (13) ◽  
pp. 2425 ◽  
Author(s):  
Tsunehisa Hirose ◽  
Daniel Keck ◽  
Yoshihiro Izumi ◽  
Takeshi Bamba
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Supercritical Fluid ◽  
Supercritical Fluid Chromatography ◽  
High Performance ◽  
Stationary Phases ◽  
Retention Factor ◽  
Normal Phase ◽  
Retention Behavior ◽  
Selection Of

The retention behavior of a wide variety of stationary phases was compared in supercritical fluid chromatography (SFC) and normal-phase high-performance liquid chromatography (NP-HPLC). We also attempted to elucidate the retention behavior in SFC by investigating the selectivity of the different stationary phases. SFC separation conditions with polar stationary phases, such as silica gel (SL) and diol (Diol) phases, operate via adsorptions that include hydrophilic and ionic interactions similar to those in NP-HPLC. Moreover, non-polar stationary phases, such as pentabromophenyl (PBr), pyrenylethyl (PYE), and octadecyl (C18), could be used despite the non-polar mobile phase conditions, because the dispersion and π-π interactions were stronger in SFC than in HPLC. These results reflect the selectivity of the stationary phase and its retention factor, thus providing useful information for the selection of appropriate stationary phases for particular analytes.

scholarly journals Retention behaviour of some estradiol derivatives on alumina in normal phase chromatrography

2003 ◽  
Vol 68 (3) ◽  
pp. 163-170 ◽  
Author(s):  
Marijana Acanski
Keyword(s):  
Mobile Phase ◽  
Volume Fraction ◽  
Retention Behavior ◽  
Relative Retention ◽  
Linear Relationships ◽  
Mobile Phases ◽  
Binary Mobile Phases ◽  
Estradiol Derivatives ◽  
Rf Values ◽  
The Relationship

The retention constants of variously substituted estradiol derivatives were measured as a function of the composition of various binary mobile phases in order to study the relationship between chemical structure and retention behavior in TLC on alumina. The slopes and intercepts of the linear relationships between the retention constant RM and the logarithm of the volume fraction of the polar mobile phase component were calculated and are discussed in relation to the characteristics of the solute and mobile phase. The RF values and relative retention ?RM of the compounds depend largely on the retention behavior of their substituents. The results obtained in this investigation are compared with the results of the same derivatives obtained in previous investigations on silica gel.

scholarly journals The Separation of Cannabinoids on Sub-2 µm Immobilized Polysaccharide Chiral Stationary Phases

2021 ◽  
Vol 14 (12) ◽  
pp. 1250
Author(s):  
Takafumi Onishi ◽  
Weston J. Umstead
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Stationary Phases ◽  
Chiral Stationary Phases ◽  
Reversed Phase ◽  
Normal Phase ◽  
Elution Order ◽  
Testing Methods ◽  
Structural Isomers

The increased use and applicability of Cannabis and Cannabis-derived products has skyrocketed over the last 5 years. With more and more governing bodies moving toward medical and recreational legalization, the need for robust and reliable analytical testing methods is also growing. While many stationary phases and methods have been developed for this sort of analysis, chiral stationary phases (CSPs) are unique in this area; not only can they serve their traditional chiral separation role, but they can also be used to perform achiral separations. Given that mixtures of cannabinoids routinely contain enantiomers, diastereomers, and structural isomers, this offers an advantage over the strictly achiral-only analyses. This work presents the separation of a 10-cannabinoid mixture on several polysaccharide-based sub-2 µm CSPs with both normal-phase and reversed-phase ultra-high-performance liquid chromatography (UHPLC) conditions. Along with the separation of the mixture, appropriate single-peak identification was performed to determine the elution order and reported where applicable.

scholarly journals HPLC separation of panthenol enantiomers on different types of chiral stationary phases

2018 ◽  
Vol 11 (2) ◽  
pp. 114-119 ◽  
Author(s):  
Anna Lomenova ◽  
Katarína Hroboňová ◽  
Terézia Šolónyová
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Limit Of Detection ◽  
Stationary Phases ◽  
Pharmaceutical Preparations ◽  
Chiral Stationary Phases ◽  
Biologically Active ◽  
Different Types

Abstract Panthenol is a biologically active compound closely related to vitamin B5 (pantothenic acid). This work deals with the separation of panthenol enantiomers using high performance liquid chromatography. Different types of chiral stationary phases (β-cyclodextrin, isopropyl carbamate cyclofructan 6, amylose tris(3,5-dimethylphenylcarbamate)) were tested in normal phase separation mode. Effect of mobile phase composition on the resolution and retention of enantiomers was studied. Two types of detectors, low-wavelength UV and polarimetric, were used. The optimal chromatographic system includes a chiral stationary phase based on amylose and a mobile phase of hexane/ethanol (60/40, v/v) where the resolution of enantiomers reached the value Rs = 2.49. Suitable chromatographic conditions were applied for the determination of panthenol enantiomers in samples of pharmaceutical preparations with the obtained recovery of more than 92 %. Linearity of the high performance liquid chromatography method with spectrophotometric detection was from 1.0 × 10−3 to 1.3 mg mL−1 (R2 = 0.998), with the limit of detection of 0.3 × 10−3 mg mL−1 for both enantiomers.

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