scholarly journals The possibility of coriander seed disinfection with the essential oil of peppermint

2021 ◽  
Vol 66 (1) ◽  
pp. 39-52
Author(s):  
Sara Mikic ◽  
Ivana Vico ◽  
Natasa Duduk ◽  
Tatjana Markovic

Seed health testing aims to ensure a healthy crop and to prevent the spread of plant diseases. The need to study and apply both essential oils and plant extracts against seed diseases is growing and becoming more important. Coriander (Coriandrum sativum L.) is an annual herbaceous, aromatic, spicy and medicinal plant from the Apiaceae family, known for its seeds with a characteristic and pleasant odour. In human medicine, coriander is a part of various medicines for the treatment of digestive organs. Phytopathogenic fungi and bacteria are the most common and significant causes of coriander seed disease. The aim of this study was to assess coriander seed health and investigate the possibility of seed disinfection using peppermint (Mentha x piperita) essential oil. The health of the seeds was examined by the filter paper laboratory standard method. Different concentrations (1, 2, 5 and 10%) of peppermint essential oil were used for seed disinfection. The composition of the essential oil was determined by gas chromatographic analysis. The results of seed health analysis showed the presence of a fungus after seven days of incubation in a humid chamber at room temperature. The fungus was identified as Alternaria sp. (sect. Alternaria) based on the symptoms and macroscopic and microscopic features of the fungus (on seed and potato dextrose agar). Immersion treatment of infected seed in peppermint essential oil reduced the presence of Alternaria sp. on coriander seeds. The highest disease inhibition, 69,33%, namely 58,93% without the influence of the emulsifier, was shown by oil solution of 5%. Stagnation in inhibition was observed with a further increase in oil concentration. The results of this study show the possibility of disinfecting coriander seeds with peppermint essential oil to reduce infection with Alternaria sp.

2020 ◽  
Author(s):  
Theint Theint Win ◽  
Sikandar Khan ◽  
Pengcheng Fu

Abstract Background: Biogenic nanoparticles have proved to be effective biocontrol agents for certain plant diseases. It possesses the potential for extensive use for sustainable agriculture. Many attempts have been made to synthesize nano-based antifungal compounds for the management of soil borne pathogenic fungi for crops.Results: In our work, silver nanoparticles (AgNPs) was constructed with phytopathogenic fungi (Alternaria sp.) which was isolated from banana cultivated soil. Alternaria sp. was able to grow rapidly and produce highly bioactive compounds as safe antifungal agent against plant pathogenic fungi (Fusarium spp. and Alternaria sp.). The size of synthesized silver nanoparticles ranged between 5-10 nm. Analytic tools, such as UV-visible spectroscopy, Fourier transformed infra-red (FTIR) spectroscopy, scanning transmission electron microscopy (STEM), EDS and elemental mapping were used to visualize the formation of AgNPs. The UV-visible spectra showed the peak at 435 nm. The maximum inhibition zone was observed at 100 µl concentration of AgNPs for Fusarium oxysporum (21 ± 2 mm) following Alternaria sp. (20± 2 mm), suggested that the efficacy of the biosynthesized NPs against the phytopathogenic fungi.Conclusions: The resulting AgNPs showed distinct antifungal activity against selected pathogenic plant fungi. The work indicates that green reduction and biogenic synthesis of nanoparticles with benign fungi is an effective, low cost, sustainable and environmentally friendly approach for prevention of soil borne plant diseases.


2006 ◽  
Vol 49 (6) ◽  
pp. 867-871 ◽  
Author(s):  
Terezinha de Jesus Faria ◽  
Rafael Sottero Ferreira ◽  
Lidiane Yassumoto ◽  
José Roberto Pinto de Souza ◽  
Noemia Kazue Ishikawa ◽  
...  

An investigation of antifungal activity of the essential oil obtained by steam-distillation (1.1% w/w) of the aerial parts of Ocimum gratissimum and of an ethanolic extract from the steam-distillation residue was carried out using the agar diffusion method. The results revealed that the essential oil inhibited the growth of all fungi tested, including the phytopathogens, Botryosphaeria rhodina, Rhizoctonia sp. and two strains of Alternaria sp., while the extract from the residue was inactive. The essential oil was subjected to TLC bioautography used to detect fungitoxic constituents. The compound that showed antifungal activity was isolated and identified as eugenol. GC/MS analysis showed that eugenol was the main constituent of the essential oil studied. The antifungal activity of eugenol was evaluated against a species of Alternaria isolated from tomato (A1) and Penicillium chrysogenum. The minimal inhibitory concentrations of eugenol were 0.16 and 0.31 mg/disc for Alternaria sp. (A1) and P. chrysogenum, respectively.


2011 ◽  
Vol 6 (11) ◽  
pp. 1934578X1100601 ◽  
Author(s):  
Mohamed Znini ◽  
Gregory Cristofari ◽  
Lhou Majidi ◽  
Hamid Mazouz ◽  
Pierre Tomi ◽  
...  

The essential oils of the aerial parts of Asteriscus graveolens have been studied using GC and GC-MS. Twenty-eight compounds were identified in the essential oil amounting to 94.9% of the total oil. The aerial part oils showed similar chromatographic profiles and were characterized by having a high content of oxygenated sesquiterpenes with 6-oxocyclonerolidol (66.7% ±5.5) and 6-hydroxycyclonerolidol (8.8% ±1.2) as the major components. The antifungal effect of the essential oil from A. graveolens leaves was evaluated in vitro against three phytopathogenic fungi of apples ( Alternaria sp., Penicillium expansum, and Rhizopus stolonifer). The results suggest that this essential oil has fungicidal properties towards Alternaria sp. from direct contact assay at 0.2% (v/v) and to P. expansum from vapor assay tests at 80 μL.


2010 ◽  
Vol 13 (3) ◽  
pp. 29-38
Author(s):  
Ha Bich Phan ◽  
Thach Ngoc Le

Coriandrum sativum L. belongs to the Apiaceae family, which is cultivated in Dong Nai province, in this paper its seed oil of was studied. The essential oil was extracted by hydrodistillation. We used two methods for activating of hydrodistillation: conventional heating and microwave irradiating. Its physical and chemical indexes were measured. The chemical composition of this oil was identified by GC/MS and quantified by GC/FID. Linalool (75.51-77.21 %), and geranyl acetate (15.64-12.79 %) were the main constituents of the oil which were obtained in 0,32-0.39 % yield. The biological activity of this oil was reported.


Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1066
Author(s):  
Fahima Abdellatif ◽  
Muhammad Akram ◽  
Samir Begaa ◽  
Mohammed Messaoudi ◽  
Adel Benarfa ◽  
...  

This study describes the minerals elements, chemical composition, antioxidant and antimicrobial activities of Algerian Melissa officinalis plant. The essential oil (EO) was extracted by hydrodistillation (HD) using a Clevenger-type apparatus of dry leaves of M. officinalis and was analyzed by two techniques, gas chromatography coupled with flame ionization (GC-FID) and gas chromatography coupled with mass spectrometry (GC-MS). Eighteen minerals comprising both macro- and microelements (As, Br, K, La, Na, Sb, Sm, Ba, Ca, Ce, Co, Cr, Cs, Fe, Rb, Sc, Th, and Zn) were determined using neutron activation analysis technique for the first time from Algerian Melissa officinalis plant. Seventy-eight compounds were identified in the essential oil, representing 94.090% of the total oil and the yields were 0.470%. The major component was geranial (45.060%). Other predominant components were neral (31.720%) and citronellal (6.420%). The essential oil presented high antimicrobial activity against microorganisms, mainly five human pathogenic bacteria, one yeast, Candida albicans, and two phytopathogenic fungi. The results can be used as a source of information for the pharmaceutical industry and medical research.


2021 ◽  
pp. 24-27
Author(s):  
Л.М. Соколова ◽  
А.В. Янченко ◽  
А.Ю. Федосов ◽  
М.И. Азопков ◽  
В.С. Голубович

Морковь и свекла столовая – одни из самых распространенных в овощеводстве корнеплодных культур. Важный признак семян высокого качества – отсутствие болезней, передаваемых через семена. Эффективный контроль болезней растений имеет решающее значение для надежного производства овощей и потенциально может привести к значительной экономии использования воды, земли, топлива и других ресурсов в сельском хозяйстве. Присутствие в семенах патогенов, передающихся через семена, либо препятствует прорастанию, либо может привести к эпифитотиям из-за передачи возбудителя болезни от семени к растению. За последние годы отмечено увеличение числа заболеваний, вызванных фитопатогенными грибами, бактериями и вирусами. В зависимости от погодных условий и фитосанитарного состояния посевов, распространенность болезней может достигать 70-80% от всей популяции растений, а урожайность снижаться в ряде случаев на 80-98%. Цель исследований – выявить инфицированность и родовую принадлежность патокомплекса грибных болезней на семенах моркови и свеклы столовой, а также подобрать оптимальный режим обработки семян. Термическая обработка семян представляет собой жизнеспособную альтернативу химической обработке для уничтожения патогенов. Задача термической обработки – найти наилучшее сочетание продолжительности экспозиции и температуры, которое обеспечивает максимальную гибель патогенов при минимальном разрушающем воздействии на семена. Основное предположение, оправдывающее термическую обработку, заключается в том, что патоген-мишень более чувствителен к высокотемпературному стрессу, чем семена. Целевыми патогенными микроорганизмами являются в основном грибы, вирусы и бактерии, находящиеся на поверхности и внутри семян. В настоящей статье отображено влияние термической обработки семян столовой свеклы и моркови на всхожесть и их зараженность патогенами. Показана высокая эффективность термической обработки семян столовой свеклы и моркови против Alternaria. Дана визуальная оценка эффективности термической обработки на питательной среде Чапека. One of the most common root vegetables in vegetable growing are carrots and beets. One of the important signs of qualitative changes is the absence of diseases transmitted through seeds. Effective control of plant diseases is crucial for the reliable production of vegetables and can potentially lead to a significant reduction in the use of water, land, fuel and other resources in agriculture. The presence of pathogens transmitted through seeds in seeds either prevents germination, or can lead to epiphytotics of diseases due to the transmission of the pathogen from the seed to the plant. In recent years, there has been an increase in the number of diseases caused by phytopathogenic fungi, bacteria and viruses. Depending on the weather conditions and the phytosanitary condition of crops, the prevalence of diseases can reach 70-80% of the entire plant population, and the yield can decrease in some cases by 80-98%. The purpose of the research is to identify the infection and generic affiliation of the pathocomplex of fungal diseases on carrot and beet seeds, as well as to choose the optimal seed treatment regime. Heat treatment of seeds is a viable alternative to chemical treatment for the destruction of pathogens. The task of heat treatment is to find the best combination of time and temperature that maximizes the reduction in the survival of pathogens with minimal destructive effects on seeds. The main assumption justifying heat treatment is more sensitive to high-temperature stress than seeds. The target pathogenic microorganisms are mainly fungi, viruses and bacteria located on the surface and inside the seeds. This article shows the effect of heat treatment of table beet and carrot seeds on germination and their infection with pathogens. The high efficiency of heat treatment of table beet and carrot seeds against Alternariais shown. A visual assessment of the effectiveness of heat treatment on the Czapek nutrient medium was carried out.


2012 ◽  
Vol 36 (4) ◽  
pp. 383-390 ◽  
Author(s):  
Ricardo Borges Pereira ◽  
Gilvaine Ciavareli Lucas ◽  
Fabiano José Perina ◽  
Pedro Martins Ribeiro Júnior ◽  
Eduardo Alves

The rust and brown eye spot are the main coffee diseases. The losses are due to intense defoliation of plants, which has reduced its production and longevity. The brown eye spot also occurs in fruits, with negative effects on the beverage quality. Some essential oils have presented promising results in the control of plant diseases, as an alternative to the use of fungicides. The objective of this study was to evaluate citronella essential oil in the control of rust and brown eye spot and in the activation of coffee plants defense responses. Twelve-month-old plants were sprayed with citronella oil 1000 µL L-1, acibenzolar-S-methyl 200 mg L-1 and tebuconazole fungicide 200 mg L-1. Plants were inoculated with Hemileia vastatrix and Cercospora coffeicola seven days later. The application was repeated after 30 days. Plants with five months were sprayed with the same treatments to assess the induced defense responses. Citronella oil controlled rust and brown eye spot with efficiencies of 47.2% and 29.7%, respectively, while tebuconazole presented control of 96.5% and 90.5%, respectively. Acibenzolar-S-methyl reduced brown eye spot by 55.9% and showed no significant control of rust. Citronella oil increased peroxidase and chitinase activities in five months coffee plants 336, and 24 and 336 hours after spraying, respectively. Acibenzolar-S-methyl increased peroxidase, chitinase and ββ-1,3-glucanase activities 192, 288 and 336; 24 and; 240 hours after spraying, respectively. The treatments did not increase accumulation of phenols, but a significant increase in lignin was observed in plants sprayed with citronella oil.


2021 ◽  
Author(s):  
Kadriye Ozlem saygi ◽  
Birgul Kacmaz ◽  
Serdar Gul

Abstract Bacterial infections are one of the most serious health problems all over the world, which cause need for the discovery of new drug. Since antibiotic resistance is a major threat to both humans and the environment, there is a need for studies on the antimicrobial properties of different forms of traditionally used plants. Herein, the seeds of coriander were used to isolate essential oil (EO) and to synthesise silver nanoparticles (C-AgNPs). The major oil constituents were characterized by GC-MS as Linalool (79.12%), Camphor (6.16%), γ-Terpinene (2.82%) and α-Pinene (2.67%). The synthesized AgNPs were characterized by UV-Visible spectrophotometry, Scanning Electron Microscopy (SEM), Energy Dispersive X-ray (EDX), X-ray difraction (XRD) and Fourier Tranmission Infrared (FTIR). The surface plasmon resonance (SPR) of C-AgNPs at 437 nm was recorded on the UV-Vis spectrometer. The spherical and homogenous of AgNPs was presented in SEM images. EDX showed elemental distribution and confirmed AgNPs. A characteristic intense peak was at 3.0 keV. The antibacterial activities of the essential oil and AgNPs form of coriander seed against Staphylococcus aureus ATCC 29213 and Escherichia coli ATCC 25922 bacteria were investigated by broth microdilution test. AgNPs and essential oil of coriander can be expected to provide future opportunities in nanomedicine and materials science. AgNPs can be displayed synergistic antimicrobial effect when used in combination with essential oil.


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