scholarly journals Changes in alfalfa cell wall structure during vegetation

2014 ◽  
Vol 59 (3) ◽  
pp. 275-286
Author(s):  
Aleksa Bozickovic ◽  
Goran Grubic ◽  
Nenad Djordjevic ◽  
Bojan Stojanovic

The investigation was done on 141 samples of one alfalfa cultivar, collected from the same location during the first three growth cycles: spring growth, the first and the second regrowth. Within each growth cycle, sampling was done during the whole growing period, commencing when plant height was below 150 mm and continuing until plants were bearing ripe seeds. On all collected samples the following cell wall characteristics were determined: neutral detergent fibre (NDF), acid detergent fibre (ADF), acid detergent lignin (ADL), neutral detergent insoluble crude protein (NDICP), acid detergent insoluble crude protein (ADICP). Cellulose and hemicellulose were detected on the base of the mentioned chemical parameters. Significantly lower (p<0.01) content of aNDF, ADF, ADL, ADICP and cellulose is found in the second regrowth, while there were no significant differences between the other two growth cycles. Except in NDICP and ADICP, the increase in all accompanying components of the cell wall was observed, and expressed in average daily changes. There was no consistent trend in NDICP and ADICP. During the spring growth from late bud to full-bloom stage the ?plateau? was observed. The plateau was represented as almost constant content of aNDF, ADF, ADL and cellulose. The correlations between all components of the cell wall were shown. The equation aNDF = 36.713 + 1.181 ? ADF is recommended for conversion of ADF into aNDF in alfalfa.

2020 ◽  
Vol 47 (1) ◽  
pp. 246-256
Author(s):  
N. J. Ekanem ◽  
U. Okah ◽  
F. O. Ahamefule ◽  
O. J. Ifut ◽  
K. Ikwunze ◽  
...  

Enterolobium cyclocarpum leaves are less acceptable by ruminants due to their high contents of anti-nutritional factors. The effect of preserving Enterolobium cyclocarpum leaves as hay and silage or silage: hay combinations on the proximate, fibre fractions, anti-nutritional factors, minerals and vitamins compositions were investigated in this study. Six experimental treatments consisting of 100% fresh Enterolobium cyclocarpum leaves, 100% ensiled Enterolobium cyclocarpum leaves, 100% sun dried Enterolobium cyclocarpum leaves, 75% silage: 25% hay, 50% silage: 50% hay and 25% silage: 75% hay were formulated. Each treatment was replicated 3 times and analyzed for chemical composition. The crude protein content ranged from 14.70 – 22.05%, which significantly differed (p<0.05) across treatments with the highest value in the fresh leaves. The values for neutral detergent fibre ranged from 56.64 – 57.92 %, acid detergent fibre from 37.14 – 39.87 % and acid detergent lignin from 13.30 – 14.96 %, they all differed significantly (p<0.05) across treatments with highest values in the 100% ensiled treatment. Preservation as silage significantly (p<0.05) reduced the concentrations of tannin, phytate and hydrocyanic acid, while preservation as hay significantly (p<0.05) reduced the concentration of saponin. The mineral contents of the EC leaves preserved as silage, hay and silage: hay combinations (Treatments 2 - 6) compared favourably with the mineral contents of the fresh leaves (Treatment 1). Preserved EC leaves (Treatments 2 – 6) had significantly (p<0.05) higher concentrations of vitamin C (444.21 –657.60 mg/100g) than the fresh leaves (308.79mg/100g). Thus preservation of E. cyclocarpum leaves as silage or hay improved its chemical composition and reduced its contents of anti-nutritional factors.


2020 ◽  
Vol 45 (4) ◽  
pp. 203-209
Author(s):  
U. Okpanachi ◽  
C. I. Agu ◽  
L. E. Igoche ◽  
F. A. Oyedapo

The study was aimed at assessing the effects of two varieties of cashew (Anacardium occidentale) apple pulp as feedstuff for ruminants. Samples of the yellow and red varieties of cashew apple pulp were collected, processed and analysed for mineral, crude fiber fraction and pH. Results showed that sodium value in red variety was significantly higher (P<0.05) than that of the yellow variety. Potassium value in red variety was significantly higher (P<0.01) than that of the yellow variety. Values for calcium, magnesium, and phosphorus in the yellow variety were significantly higher (P<0.01) than those of the red variety. Values for cellulose, hemicelluloses and neutral detergent fibre in the red variety were significantly higher (P<0.01) than those of the yellow variety. Acid Detergent Lignin value for the yellow variety was significantly higher (P<0.01) than that of the red variety. Values for acid detergent fibre, crude protein, carbohydrate and pH were not significantly affected (P>0.05). Yellow cashew apple pulp from the results of this study is richer in minerals when compared to the red variety. Results from the crude fibre fraction suggest that the yellow cashew apple pulp variety may have a better digestibility when compared to the red variety. Although the values of the various parameters from the 2 varieties show that they are fit to be used as feeding stuff for ruminants, the yellow variety appears to be better on the general basis.


1991 ◽  
Vol 31 (3) ◽  
pp. 341 ◽  
Author(s):  
GF Craig ◽  
DT Bell ◽  
CA Atkins

The forage potential of 11 species of Acacia from naturally saline areas of Western Australia was assessed by analysing their nutritive characteristics and acceptability to sheep. The crude protein content of new shoots was greater than that of mature phyllodes [8-25 v. 6-12% dry matter (DM) respectively]. This was reflected in higher in vitro DM digestibility (IVDMD) values (34-59% in shoots v. 22-54% in phyllodes). IVDMD appeared to be reduced by high concentrations of neutral and acid detergent fibre (NDF and ADF) and acid detergent lignin (ADL). The least acceptable species generally had high values of NDF. The most promising species for use as perennial fodder shrubs were Acacia ampliceps, A. brunzalis, A. cyclops and A. ligustrina. Mature phyllodes of these species had IVDMD >40%, ADL <20% and crude protein level of all foliage in the range 8-17% DM. A. patagiata and A. ixiophylla were acceptable to sheep; however, crude protein levels and IVDMD of phyllodes were relatively low. Although new shoot material of A. eremaea, A. inceae, A. aff. lineolata, A. mutabilis and A. redolens had relatively high levels of crude protein, mature phyllodes of these species had low levels of protein, IVDMD <40% and high NDF concentrations (>50% DM), which suggests that these species would be unsuitable as animal feed. The ability of salt-tolerant acacias to grow in marginal land means they may provide supplementary forage in times of drought.


Author(s):  
Jasmine Kaur ◽  
S. Thakur ◽  
Mahendra Singh

The aim of the present study was to determine the nutritional value of Phalaris minor seeds in comparison to conventional cereal grains. P. minor seeds had higher ether extract (6.3%), total ash (7.5%), acid insoluble ash (AIA, 4.5%), acid detergent fibre (ADF, 10.2%), cellulose (5.5%), acid detergent lignin (ADL, 2.0%), neutral detergent insoluble crude protein (NDICP, 4.0%) and acid detergent insoluble crude protein (ADICP, 2.7%) content in comparison to conventional cereal grains. The crude protein of P. minor seeds (12.3%) was similar to that of wheat (12.0%), but higher than other conventional cereal grains including maize (9.0%). The amino acid composition of P. minor seeds was more or less similar to conventional cereal grains. P. minor seeds had a superior mineral profile (Ca, P, Na, K, Mg, Fe, Cu, Zn and Mn) than conventional cereal grains. In vitro evaluation revealed that net gas production was lowest (P less than 0.05) in P. minor seeds (216.37 L/kg DM/24 h). The methane production (L/kg DM/24 h)from P. minor seeds (43.03)was lower (P less than 0.05) than that from wheat (54.33) and barley (57.35). The organic matter digestibility of P. minor seeds (83.27%) was similar to that of barley (81.69%).It was concluded that P. minor seeds could be considered as potential source of nutrients for livestock.


1929 ◽  
Vol 12 (3) ◽  
pp. 329-344
Author(s):  
T. Brailsford Robertson

1. It has previously been shown by the author and many others that growth, in animals and plants, is an autocatalysed process. In animals it is usual to find that growth occurs in several superimposed autocatalytic cycles. In many cases, in plants and animals, especially if the cycle is one which occupies a large proportion of the growing period, it is found that the velocity-constant of the autocatalysed monomolecular formula falls off as growth proceeds, at first rapidly and later more slowly. 2. It has previously been shown by the author that the fall of the velocity-constant of growth, in the white mouse, is directly proportional to the fall of the nucleo-cytoplasmic ratio, determined by the chemical method of Le Breton and Schaeffer. If we assume this relationship to be generally applicable to the growth of animals and plants, then the following additional conclusions may be deduced, without calling in the aid of any other assumption:— 3. The increase of cytoplasm in any given cycle of growth is proportional to the concurrent increase of nuclear material. 4. The growth of cytoplasm takes place in accordance with a monomolecular formula in which the velocity-constant varies directly as the mass of the nucleus. If we superadd to these facts and deductions the hypothesis that each growth-cycle represents the growth of a separate group of cells within the animal, then the additional conclusions follow:— 5. That the cells which participate in the growth composing any cycle have initially lower nucleo-cytoplasmic ratios than the cells which participated in the preceding cycles. 6. That cells of large nucleo-cytoplasmic ratios in a multicellular animal inhibit the growth of cells which possess smaller ratios. 7. These conclusions collectively imply that the nucleus plays a predominant role in determining the development of the cell in which it resides.


2008 ◽  
Vol 53 (No. 12) ◽  
pp. 499-505 ◽  
Author(s):  
P. Homolka ◽  
V. Koukolová ◽  
Z. Němec ◽  
Z. Mudřík ◽  
B. Hučko ◽  
...  

Lucerne (<I>Medicago sativa</I> L. var. Palava), harvested at four successive dates over a 30-day period, was evaluated for chemical composition (dry matter, organic matter, crude protein, ether extract, crude fibre, nitrogen-free extract, neutral detergent fibre, acid detergent fibre, acid detergent lignin), amino acid contents and intestinal digestibility in dairy cows. Dry matter (<I>r</I> = 0.78), organic matter (<I>r</I> = 0.95), crude fibre (<I>r</I> = 0.91), neutral detergent fibre (<I>r</I> = 0.94), acid detergent fibre (<I>r</I> = 0.79) and acid detergent lignin (<I>r</I> = 0.48) presented positive linear correlation coefficients (<I>r</I>) with growth stage, whereas crude protein (<I>r</I> = –0.96), ether extract (<I>r</I> = –0.86) and nitrogen-free extract (<I>r</I> = –0.70) showed negative relationships. Total essential amino acid content decreased (<I>r</I> = –0.94) from 84.1 to 55.3 g/kg of dry matter with maturity, with <I>r</I>-values higher than –0.90 obtained between growth stage and contents of lysine, methionine, threonine and valine. With the exception of tyrosine (<I>r</I> = –0.68), r-values between growth stage and individual non-essential amino acids were all higher than –0.90. Total amino acid (<I>r</I> = –0.98) and nitrogen (<I>r</I> = –0.99) contents presented comparable tendencies with successive sampling times. Whereas no definite trends were detected for the amino acid composition of rumen incubated (16 hours) lucerne samples, the intestinal digestibility of total essential (<I>r</I> = –0.78), total non-essential (<I>r</I> = –0.58) and total (<I>r</I> = –0.69) amino acids as well as nitrogen (<I>r</I> = –0.99) decreased with growth. However, due to a small sample size (<I>n</I> = 4), most linear relationships between constituents and growth stage were insignificant. It can be concluded that, although limited in the sample size, this report presents information on the decrease in amino acid contents and intestinal amino acid digestibility as growth proceeds in lucerne (var. Palava) produced in the Czech Republic, which could be utilized in the feeding of ruminants.


2020 ◽  
Vol 50 (1) ◽  
pp. 9-16 ◽  
Author(s):  
H Çayıroğlu ◽  
G Filik ◽  
İ Coşkun ◽  
A Gül Filik ◽  
H Çayan ◽  
...  

This study was conducted to determine the effects of spraying oregano essential oil (OEO) onto sugar beet pulp silage (SBPS) on silage quality and aerobic stability after opening. A factorial experiment with three replicates of three treatments and four time periods was conducted using laboratory-type plastic silos. The treatments were an untreated control, silage sprayed with 10 ml/75 cm2 OEO, and silage sprayed with 20 ml/75 cm2 OEO. The silages were sampled at 0, 72, 120, and 168 hours after spraying. Temperature, L*, a*, and b* colour values, pH, water-soluble carbohydrates, crude nutrient contents, Fleig score, metabolizable energy (ME) value, lactic acid bacteria (LAB), total live bacteria (TLB), yeast, and mould formation were assessed. Spraying OEO onto SBPS did not affect L*, a*, and b* values, pH, water-soluble carbohydrates, and Fleig score values, but decreased temperature. Spraying OEO onto the silage increased organic matter, ether extract, acid detergent fibre, neutral detergent fibre and acid detergent lignin contents without affecting crude protein, crude fibre, nitrogen free extract, and ME contents. Irrespective of treatment, crude protein, ether extract, acid detergent fibre, acid detergent lignin, nitrogen free extract, Fleig score, and ME contents of silages increased with time after spraying. The OEO spraying reduced LAB, TLB, and yeast contents in silages. In conclusion, OEO spraying onto opened SBPS reduced LAB, TLB, and yeast formation and stopped mould growth up to 72 hours without affecting their nutritional properties, suggesting that OEO could be used to ensure the stability of SBPS. Keywords: aerobic stability, mould growth, silage microbiology, nutrients, feeding management


2020 ◽  
Vol 28 (s1) ◽  
pp. 103-115
Author(s):  
D. Kereilwe ◽  
V.E. Emongor ◽  
O. Oagile ◽  
O. Phole

Safflower (Carthamus tinctorius L.) is a multipurpose crop, grown for its high quality edible oil (poly unsaturated and monounsaturated fatty acids), and as a horticultural crop (vegetable and cut flower). It is also grown for flavouring and colouring foods, as herbal tea, livestock feed, pharmaceuticals, dyes, paints and biodiesel.The objective of this study wasto evaluate the influence of safflower genotype on the nutritional quality of whole seed used as livestock feed. Nine safflower genotypes (eight exotic and one local) were grown in winter and summer. The seeds were harvested at physiological maturity and analysed for different nutritional variables. Whole safflower seeds dry matter (DM), crude protein (CP), neutral detergent fibre (NDF), acid detergent fibre (ADF), acid detergent lignin (ADL) and ash varied significantly, depending on genotype and growing season. The seed mineral content (P, K, Mg and Na) also varied across genotypes. Safflower whole seeds have great potential to serve as an excellent livestock feed in semi-arid conditions.


2008 ◽  
Vol 52 (No. 10) ◽  
pp. 348-353
Author(s):  
B. Písaříková ◽  
J. Peterka ◽  
M. Trčková ◽  
J. Moudrý ◽  
Z. Zralý ◽  
...  

Twenty samples of the aboveground biomass of <i>Amaranthus cruentus</i> (varieties Olpir, Amar 2 RR-R 150, and A 200 D) and <i>A. hypochondriacus</i> (variety No. 1008) were analysed to determine their nutritional value during the experimental period covering five growth stages from inflorescence emergence till full ripening of grain. During plant growth, from day 80 to day 120 of cultivation, the nutritional value decreased. This was demonstrated by an increase in insoluble fibre and a reduction in crude protein content. The content of neutral detergent fibre (NDF) in the dry matter increased from 356 to 420 g/kg (<i>P</i> < 0.01); acid detergent fibre (ADF) from 238 to 286 g/kg (<i>P</i> < 0.01); acid detergent lignin (ADL) from 22.9 to 53.4 g/kg (<i>P</i> < 0.01). The content of crude protein in the dry matter of the investigated amaranth varieties decreased from 174 to 109 g/kg (<i>P</i> < 0.01). In the Czech Republic the aboveground biomass of the cultivated <i>A. cruentus</i> and <i>A. hypochondriacus</i> could be used as a good source of fibre and protein in animal diets when harvested in the period from day 80 to 90 of cultivation.


2021 ◽  
Vol 38 (1) ◽  
pp. 55-63
Author(s):  
T. E. Lawal ◽  
E .A Iyayi ◽  
B. A . Adeniyi ◽  
O. Adaramoye

This study was carried out to investigate changes in proximate, sugars and cell wall components of cassava root sieviates (CRS) treated with extracted enzymes obtained from Aspergillus niger (An), Trichoderma viridae (Tv), Rhizopus stolonifer (Rs) and Mucor mucedo (Mm) applied on autoclaved CRS at 250ml/kg. Another treatment that had a commercial enzyme Roxazyme G2G (RG2G) as the degrading agent was prepared and the enzyme was applied at 150g/tonne as recommended by the manufacturer. At the end of the7 thday after enzyme application, the chemical analysis showed a higher bioavailability of nutrients in the degraded samples than the undegraded sample. Non starch polysaccharides (NSPs) in the CRS was negatively related to crude protein, ash and metabolisable energy (P<0.05) but positively related to crude fibre, pectin, neutraldetergent fibre (NDF), acid detergent fibre (ADF), acid detergent lignin (ADL), hemicellulose and cellulose content . The highest value of metabolisable energy (ME), Ash, crude protein and phosphorus were : 2807.81kcal/kg, 17.32,18.32 and 0.91 g/100gdry matter respectively; obtained when A.niger, T.viridae, A. niger and R. stolonifer enzymes were added respectively .The lowest values of crude fibre, pectin, NDF,ADF, ADL, hemicellulose and cellulose were: 4.82,3.52,30.74, 17.12, 3.74,13.71 and 14.46g/100g dry matter respectively obtained when enzymes extracted from A.niger , R. stolonifer, T. viride ,A. niger, R. stolonifer, M. mucedo and T. viride were applied . Results of levels of sugars (µg/ml) in undegraded CRS showed that glucose level increased by 72.40% (257.34 to 932.22), 67.18%(257.34 to 784.20), 53.75%(257.34 to 556.40), 50.13%(257.32 to 516.06) and 32.53%(257.34 to 381.43) when enzyme preparations from A. niger, T.viridae , R.stolonifer, M.mucedo, and Roxazyme G2G were applied on CRS for degradation respectively . Other sugars that were significantly (P<0.05) increased after biodegradation were galactose, fructose and sucrose. Significant (P<0.05) differences were also expressed in the mineral analysis. After biodegradation, the degraded CRS had better mineral bioavailability as there were improvements in the minerals quantity. The results revealed that the use of enzymes from the above named fungi and the commercial enzymes defiberised the CRS and hydrolyzed the cell walls and hence promoted better availability of energy, crude proteins and other nutrients which were hitherto unavailable.


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