scholarly journals Fractionation of the mixture obtained by the enzymatic hydrolysis of penicillin G

2002 ◽  
Vol 56 (9) ◽  
pp. 386-391 ◽  
Author(s):  
Dan Cascaval ◽  
Corneliu Oniscu ◽  
Anca-Irina Galaction
Keyword(s):  
Enzymatic Hydrolysis ◽  
Ph Value ◽  
Molar Concentration ◽  
Reactive Extraction ◽  
Penicillin G ◽  
Flow Sheet ◽  
Extraction Degree ◽  
Process Flow Sheet ◽  
The Individual ◽  
Hydrolysis Of

Using the experimental data on the individual physical and reactive extraction of 6APA with 1,2-dichlororethane, D2EHPA and Amberlite LA-2 together with data on the influence of the pH value of the aqueous phase and the Amberlite LA-2 concentration on the selective separation of 6APA, PG and PAA from a mixture, a process flow sheet for the fractionation of the solution obtained by the enzymatic hydrolysis of PG has been elaborated and applied. Thus, at pH=10, 6APA was selectively separated by four extraction stages, the overall extraction degree being 98.8%. PG was selectively extracted from the raffinate at pH=6 by three extraction stages, adjusting the molar concentration of Amberlite LA-2 to the value of the PG molar concentration for each extraction stage. The antibiotic extraction degree was 99.6%.

Enzymatic hydrolysis of penicillin G in ionic liquids

2008 ◽  
Vol 136 ◽  
pp. S391
Author(s):  
Mai Ngoc Lan ◽  
Sung Ho Ha ◽  
Yoon-Mo Koo
Keyword(s):  
Ionic Liquids ◽  
Enzymatic Hydrolysis ◽  
Penicillin G ◽  
Hydrolysis Of

Optimization of olive pomace enzymatic hydrolysis for fermentable sugar production

2016 ◽  
Vol 46 (6) ◽  
pp. 778-790 ◽  
Author(s):  
Ghassan Abo Chameh ◽  
Fadi Kheder ◽  
Francois Karabet
Keyword(s):  
Enzymatic Hydrolysis ◽  
Ph Value ◽  
Maximum Yield ◽  
Olive Pomace ◽  
Mixing Rate ◽  
Content Type ◽  
Hydrolysis Yield ◽  
Pretreatment Time ◽  
Hydrolysis Conditions ◽  
Hydrolysis Of

Purpose The purpose of this paper was to find out the appropriate enzymatic hydrolysis conditions of alkali pretreated olive pomace (OP) which enable maximum yield of reducing sugar. Design/methodology/approach The commercial enzymatic preparation (Viscozyme® L) was used for the hydrolysis of OP. The effects of pretreatment, time, temperature, pH, enzyme quantity and substrate loading on the hydrolysis yield were investigated. Findings This study showed that enzymatic hydrolysis of OP using Viscozyme® L can be successfully performed at 50°C. Alkaline pretreatment step of OP prior the enzymatic hydrolysis was indispensable. The hydrolysis yield of alkaline pretreated OP was 2.6 times higher than the hydrolysis yield of untreated OP. Highest hydrolysis yield (33.5 ± 1.5 per cent) was achieved after 24 h using 1 per cent (w/v) OP load in the presence of 100 μl Viscozyme® L at 50°C and pH 5.5 with mixing rate of 100 rpm (p = 0.05). Originality/value Reaction time, temperature, pH value and enzyme quantity were found to have a significant effect on enzymatic hydrolysis yield of alkali pretreated of OP. Although high-solid loadings of OP lowered the hydrolysis yield, it produced higher concentration of reducing sugars, which may render the OP conversion process more economically feasible.

Improving the Industrial Production of 6-APA: Enzymatic Hydrolysis of Penicillin G in the Presence of Organic Solvents

2003 ◽  
Vol 19 (6) ◽  
pp. 1639-1642 ◽  
Author(s):  
O. Abian ◽  
C. Mateo ◽  
G. Fernandez-Lorente ◽  
J.M. Guisan ◽  
R. Fernandez-Lafuente
Keyword(s):  
Enzymatic Hydrolysis ◽  
Organic Solvents ◽  
Industrial Production ◽  
Penicillin G ◽  
Hydrolysis Of

scholarly journals PRODUCTION OF OLIGOFRUCTOSE SYRUP BY ENZYMATIC HYDROLYSIS FROM INULIN-CONTAINING CHICORY SYRUP

Food systems ◽  
2020 ◽  
Vol 3 (3) ◽  
pp. 20-24
Author(s):  
T. S. Puchkova ◽  
D. M. Pikhalo ◽  
O. M. Karasyova
Keyword(s):  
Enzymatic Hydrolysis ◽  
Ion Exchange ◽  
Ph Value ◽  
Optimal Conditions ◽  
Carbohydrate Composition ◽  
Inulin Hydrolysis ◽  
Stage Scheme ◽  
Hydrolysis Of ◽  
Dry Substance

The purpose of the work is to study the enzymatic hydrolysis of inulin-containing syrups of various purification degrees from chicory chips in the production of oligofructose to be used in dietary, diabetic and health-preventive nutrition products. It has been determined that ion exchange purification of the syrup is necessary for hydrolysis. Individual stages of ion-exchange purification are specified using a two-stage scheme: К1-А1-К2-А2 and an additional stage on the “Macronet” sorbent MN200 to stabilize pH and remove bitterness taste. Requirements for the quality of syrup for hydrolysis have been developed: pH value — 4.5–5.0; chromaticity — not more than 0.5 units opt. den.; ash — not more than 0.2%; protein — 0.5%; no bitterness taste. Optimal conditions for hydrolysis of inulin-containing syrup have been established using Novozim 960 endoinulinase (Denmark): temperature — 55–58 °C; pH — 4.7–5.2; DS (dry substance) — 19%; preparation dosage — 0.4 units. INU/g of syrup DS; time 20–24 h. A sample of oligofructose syrup was obtained using the preparation “Novozim 960.” The carbohydrate composition of oligofructose after the inulin hydrolysis was determined: fructooligosaccharides (FOS) — 70.12%; oligofructosides — 24.79%; disaccharides — 2.11%; fructose — 2.98%. Requirements for carbohydrate composition of oligofructose obtained by enzymatic hydrolysis of inulin-containing syrup have been developed: sum total of FOS and oligosaccharides — not less than 93%, sum total of di- and monosaccharides — not more than 7%.

Extraction and enzymatic hydrolysis of Penicillin G in emulsion liquid membranes

1996 ◽  
Vol 208 (1) ◽  
pp. 133-144 ◽  
Author(s):  
I. Miesiąc ◽  
K. Schügerl ◽  
A. Hasler ◽  
J. Szymanowski
Keyword(s):  
Enzymatic Hydrolysis ◽  
Penicillin G ◽  
Liquid Membranes ◽  
Emulsion Liquid Membranes ◽  
Hydrolysis Of
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