scholarly journals Genetic-phytohormonal interactions in male fertility and male sterility phenotype expression in sunflower (Helianthus annuus L.)

Helia ◽  
2008 ◽  
Vol 31 (48) ◽  
pp. 39-46
Author(s):  
M. Duca
Keyword(s):  
Male Sterility ◽  
Helianthus Annuus ◽  
Male Fertility ◽  
Helianthus Annuus L ◽  
Male Sterility Phenotype ◽  
Phenotype Expression

scholarly journals Organization Features of the Mitochondrial Genome of Sunflower (Helianthus annuus L.) with ANN2-Type Male-Sterile Cytoplasm

Plants ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 439 ◽  
Author(s):  
Maksim S. Makarenko ◽  
Alexander V. Usatov ◽  
Tatiana V. Tatarinova ◽  
Kirill V. Azarin ◽  
Maria D. Logacheva ◽  
...  
Keyword(s):  
Mitochondrial Genome ◽  
Male Sterility ◽  
Helianthus Annuus ◽  
Candidate Genes ◽  
High Throughput Sequencing ◽  
De Novo ◽  
Simultaneous Action ◽  
Male Sterile ◽  
Helianthus Annuus L ◽  
Sunflower Line

This study provides insights into the flexibility of the mitochondrial genome in sunflower (Helianthus annuus L.) as well as into the causes of ANN2-type cytoplasmic male sterility (CMS). De novo assembly of the mitochondrial genome of male-sterile HA89(ANN2) sunflower line was performed using high-throughput sequencing technologies. Analysis of CMS ANN2 mitochondrial DNA sequence revealed the following reorganization events: twelve rearrangements, seven insertions, and nine deletions. Comparisons of coding sequences from the male-sterile line with the male-fertile line identified a deletion of orf777 and seven new transcriptionally active open reading frames (ORFs): orf324, orf327, orf345, orf558, orf891, orf933, orf1197. Three of these ORFs represent chimeric genes involving atp6 (orf1197), cox2 (orf558), and nad6 (orf891). In addition, orf558, orf891, orf1197, as well as orf933, encode proteins containing membrane domain(s), making them the most likely candidate genes for CMS development in ANN2. Although the investigated CMS phenotype may be caused by simultaneous action of several candidate genes, we assume that orf1197 plays a major role in developing male sterility in ANN2. Comparative analysis of mitogenome organization in sunflower lines representing different CMS sources also allowed identification of reorganization hot spots in the mitochondrial genome of sunflower.

scholarly journals Male sterility in sunflower (Helianthus annuus L.) induced with new gametocidal agents

2019 ◽  
Vol 24 ◽  
pp. 26-32
Author(s):  
O. V. Bilynska ◽  
V. S. Lyutenko ◽  
P. G. Dulniev ◽  
V. P. Bezpartochna
Keyword(s):  
Bioorganic Chemistry ◽  
Male Sterility ◽  
Helianthus Annuus ◽  
Female Gametophyte ◽  
Seed Set ◽  
Pollen Sterility ◽  
Helianthus Annuus L ◽  
Investigation Methods ◽  
Negative Effect ◽  
Stock Solutions

Aim. Evaluation of the new gametocidal agents effect on pollen sterility and viability of female gamethophyte in sunflower was the main goal ofthe investigation. Methods. Sunflower florets were treated with nine newly synthesided in the Institute of Bioorganic Chemistry and Petrochemistry of NAS of Ukraine chemicals in concentrations ranged from 1.0 ml/l to 15.0 ml/l of stock solutions. Gibberellic acid (50 mg/l) was used as a control. The gametocidal effect on the pollen sterility was determined using seed-set after gametocide treatment and controlled self-pollination compared to untreated florets. Influence of gameticides on the female gametophyte viability was evaluated as seed-set after free cross-pollination. Results. It was revealed that DGK-2 and DGK-3 have a high gametocidal activity even at the lowest dose – 1 ml/l. DSK-10 appeared to be the most effective one at a dose of 5 ml/l. This treatment allowed us to achieve near-complete male sterility. Negative effect of gametocides on the female gametophyte was enhanced with increase in their concentrations. However, new gametocides had less negative effect compared to gibberellin in certain experimental variants. Conclusions. DGK-2, DGK-3 and DSK-10 are promising for further investigations on the chemical demasculation in sunflower for further hybridization on the fertile basis without mechanical anther removal. Keywords: sunflower, (Helianthus annuus L.), induced pollen sterility, gamitocidal agents, gibberellin, seed-set.

Diversity among sources of cytoplasmic male sterility in sunflower (Helianthus annuus L.)

Euphytica ◽  
1991 ◽  
Vol 55 (2) ◽  
pp. 125-129 ◽  
Author(s):  
F. W. J. Havekes ◽  
J. F. Miller ◽  
C. C. Jan
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Helianthus Annuus ◽  
Helianthus Annuus L
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