scholarly journals Diversification of yellow-flowered Sempervivum (crassulaceae) species from the Balkan Peninsula: Evidence from the morphometric study of the epidermal structures of rosette leaves

2021 ◽  
Vol 45 (2) ◽  
pp. 163-176
Author(s):  
Maja Jovanovic ◽  
Dmitar Lakusic ◽  
Branislava Lakusic ◽  
Bojan Zlatkovic
Keyword(s):  
Glandular Trichomes ◽  
Balkan Peninsula ◽  
Epidermal Cells ◽  
Descriptive Statistics ◽  
Morphometric Study ◽  
Multivariate Statistical ◽  
Quantitative Characters ◽  
Univariate Anova ◽  
Taxonomic Characterization

Several related yellow-flowered houseleek species which occur on the Balkan Peninsula are divided into two complexes: Sempervivum ciliosum (S. ciliosum, S. jakucsii, S. klepa, S. octopodes, and S. galicicum) and the S. ruthenicum complex (S. ruthenicum, S. leucanthum, S. kindingeri, and S. zeleborii). Due to strong phenotypic plasticity and a limited number of studies, it is difficult to assert at this point whether all the above species are well defined in the taxonomic sense. Detailed studies of the epidermal structures have not been conducted for any of the species in either complex. The aim of this study was to investigate the degree of variability of the epidermal structures together with their potential usefulness for the taxonomic characterization of the species studied. A total of 18 quantitative characters of the epidermal structures of the adaxial and abaxial surfaces of the rosette leaves were analysed within 16 populations. In all species, the epidermal cells are polygonal or irregularly shaped, with straight or sinuous anticlinal walls, while the rosette leaves are amphistomatic with anisocytic stomata. Simple biseriate multicellular glandular trichomes were found on the adaxial and abaxial surfaces and the margins of the rosette leaves. The results of the descriptive statistics, univariate (ANOVA) and multivariate statistical analysis (CDA, AHC) showed low to high variability in the epidermal cells, guard cells and trichomes. The multivariate analysis showed diversification among the complexes and species. The length of the marginal and apical trichomes of the rosette leaves contributed most to diversification.

scholarly journals Foliar anatomical studies of Andropogon gayanus - Andropogon tectorum complex in Southwestern Nigeria

2021 ◽  
Vol 13 (4) ◽  
pp. 11031
Author(s):  
Funmilola M. OJO ◽  
Constance C. NWOKEOCHA ◽  
Julius O. FALUYI
Keyword(s):  
Leaf Blade ◽  
Glandular Trichomes ◽  
Epidermal Cells ◽  
Diagnostic Feature ◽  
Southwestern Nigeria ◽  
Anatomical Studies ◽  
Qualitative And Quantitative ◽  
Quantitative Characters ◽  
Median Part ◽  
Andropogon Gayanus

Foliar epidermal studies were carried out on accessions of Andropogon gayanus-Andropogon tectorum complex collected in Southwestern Nigeria with a view to providing additional characters of the two species of Andropogon to enhance the understanding of the taxonomic relationship between the two species. The epidermal preparation of the adaxial and abaxial surfaces of the leaf blade was made from the median part of well-matured leaf samples by the scrapping method. The analysis of both qualitative and quantitative characters revealed that study revealed that the costal zones of both adaxial and abaxial surfaces of all accessions studied showed similar features with little or no variation in their expression; epidermal cells are mostly rectangular with wavy walls, and the stomata encountered are paracytic. There was a unique occurrence of cluster of cells at the base of the macro hairs present in A. gayanus which is a diagnostic feature for its accessions collected. Kiwani, an unidentified polyploid accession, has the highest number of bands, the stomata are bigger, which is consistent with gigas effect occasioned by its polyploidy status. Glandular trichomes were present in both diploid and tetraploid of A. tectorum, a diagnostic feature for the species.

scholarly journals Goniolimon dalmaticum Rchb. f. and G. tataricum (L.) Boiss. (Plumbaginaceae) in the Croatian Flora and their Distribution in the Balkan Peninsula

2016 ◽  
Vol 75 (2) ◽  
pp. 164-172 ◽  
Author(s):  
Uroš Buzurović ◽  
Sandro Bogdanović ◽  
Marjan Niketić ◽  
Gordana Tomović
Keyword(s):  
New Species ◽  
Statistical Analysis ◽  
Morphometric Analysis ◽  
Multivariate Statistical Analysis ◽  
Balkan Peninsula ◽  
Identification Key ◽  
Morphometric Study ◽  
Multivariate Statistical ◽  
First Time ◽  
A New Species

Abstract Goniolimon tataricum (L.) Boiss. is recognized as a new species for the Croatian flora. Besides G. dalmaticum Rchb. f., G. tataricum represents the second species of this genus in Croatia. To confirm the identity of G. tataricum as a new species, morphometric study and multivariate statistical analysis were performed on the data from four Croatian populations of G. dalmaticum and G. tataricum. Based on results of morphometric analysis, an identification key for these two species is provided. Data about distribution in the Balkan Peninsula, habitat and ecology of the newly-recorded species are also presented. Typification of G. dalmaticum was performed for the first time.

Taxonomic characterization and identification of Saccharomyces cerevisiae D8 for brandy production from pineapple

2018 ◽  
Vol 39 (4) ◽  
pp. 474-482
Author(s):  
Hoang Thi Le Thuong ◽  
Nguyen Quang Hao ◽  
Tran Thi Thuy
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Low Ph ◽  
Yeast Strains ◽  
Biological Studies ◽  
Taxonomic Characterization

Eight yeast strains (denoted as D1 to D8) were isolated from samples of natural fermented pineapple. Strain D8 showed highest alcoholic production at low pH and special aroma of pineapple has been chosen for further study. Taxonomic characterization of strain D8 using morphological, biochemical and molecular biological studies confirmed that strain D8  belong to Saccharomycetaceae family, Saccharomycetales order and Saccharomyces cerevisiae species. Therefore, we named this strain as Saccharomyces cerevisiae D8 for further study on Brandy production from pineapple. Citation: Hoang Thi Le Thuong, Nguyen Quang Hao, Tran Thi Thuy, 2017. Taxonomic characterization and identification of Saccharomyces cerevisiae D8 for brandy production from pineapple. Tap chi Sinh hoc, 39(4): 474- 482. DOI: 10.15625/0866-7160/v39n4.10864.*Corresponding author: [email protected] Received 5 December 2016, accepted 12 August 2017

scholarly journals Biochemical and Taxonomic Characterization of Novel Haloarchaeal Strains and Purification of the Recombinant Halotolerant α-Amylase Discovered in the Isolate

2020 ◽  
Vol 11 ◽  
Author(s):  
Dipesh Kumar Verma ◽  
Gunjan Vasudeva ◽  
Chandni Sidhu ◽  
Anil K. Pinnaka ◽  
Senthil E. Prasad ◽  
...  
Keyword(s):  
Taxonomic Characterization

scholarly journals STATISTICAL MULTIVARIATE ANALYSIS APPLIED TO ENVIRONMENTAL CHARACTERIZATION OF SOIL IN SEMIARID REGION1

2019 ◽  
Vol 32 (1) ◽  
pp. 200-210
Author(s):  
Antônio Italcy de Oliveira Júnior ◽  
Luiz Alberto Ribeiro Mendonça ◽  
Sávio de Brito Fontenele ◽  
Adriana Oliveira Araújo ◽  
Maria Gorethe de Sousa Lima Brito
Keyword(s):  
Organic Matter Content ◽  
Principal Component ◽  
Matter Content ◽  
Multivariate Statistical ◽  
Statistical Tool ◽  
Environmental Characterization ◽  
Hierarchical Grouping ◽  
Two Factors ◽  
Total Data

ABSTRACT Soil is a dynamic and complex system that requires a considerable number of samples for analysis and research purposes. Using multivariate statistical methods, favorable conditions can be created by analyzing the samples, i.e., structural reduction and simplification of the data. The objective of this study was to use multivariate statistical analysis, including factorial analysis (FA) and hierarchical groupings, for the environmental characterization of soils in semiarid regions, considering anthropic (land use and occupation) and topographic aspects (altitude, moisture, granulometry, PR, and organic-matter content). As a case study, the São José Hydrographic Microbasin, which is located in the Cariri region of Ceará, was considered. An FA was performed using the principal component method, with normalized varimax rotation. In hierarchical grouping analysis, the “farthest neighbor” method was used as the hierarchical criterion for grouping, with the measure of dissimilarity given by the “square Euclidean distance.” The FA indicated that two factors explain 75.76% of the total data variance. In the analysis of hierarchical groupings, the samples were agglomerated in three groups with similar characteristics: one with samples collected in an area of the preserved forest and two with samples collected in areas with more anthropized soils. This indicates that the statistical tool used showed sensitivity to distinguish the most conserved soils and soils with different levels of anthropization.

scholarly journals Development and Validation of a Nested-PCR-Denaturing Gradient Gel Electrophoresis Method for Taxonomic Characterization of Bifidobacterial Communities

2003 ◽  
Vol 69 (11) ◽  
pp. 6380-6385 ◽  
Author(s):  
R. Temmerman ◽  
L. Masco ◽  
T. Vanhoutte ◽  
G. Huys ◽  
J. Swings
Keyword(s):  
Nested Pcr ◽  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Temporal Changes ◽  
Rrna Gene ◽  
Specific Pcr ◽  
Gradient Gel Electrophoresis ◽  
Species Specific ◽  
Taxonomic Characterization

ABSTRACT The taxonomic characterization of a bacterial community is difficult to combine with the monitoring of its temporal changes. None of the currently available identification techniques are able to visualize a “complete” community, whereas techniques designed for analyzing bacterial ecosystems generally display limited or labor-intensive identification potential. This paper describes the optimization and validation of a nested-PCR-denaturing gradient gel electrophoresis (DGGE) approach for the species-specific analysis of bifidobacterial communities from any ecosystem. The method comprises a Bifidobacterium-specific PCR step, followed by purification of the amplicons that serve as template DNA in a second PCR step that amplifies the V3 and V6-V8 regions of the 16S rRNA gene. A mix of both amplicons is analyzed on a DGGE gel, after which the band positions are compared with a previously constructed database of reference strains. The method was validated through the analysis of four artificial mixtures, mimicking the possible bifidobacterial microbiota of the human and chicken intestine, a rumen, and the environment, and of two fecal samples. Except for the species Bifidobacterium coryneforme and B. indicum, all currently known bifidobacteria originating from various ecosystems can be identified in a highly reproducible manner. Because no further cloning and sequencing of the DGGE bands is necessary, this nested-PCR-DGGE technique can be completed within a 24-h span, allowing the species-specific monitoring of temporal changes in the bifidobacterial community.

scholarly journals Agronomic and molecular characterization of gamma ray induced banana (Musa sp.) mutants using a multivariate statistical algorithm

Euphytica ◽  
2011 ◽  
Vol 178 (2) ◽  
pp. 151-158 ◽  
Author(s):  
Rosa Karla Nogueira Pestanana ◽  
Edson Perito Amorim ◽  
Cláudia Fortes Ferreira ◽  
Vanusia Batista de Oliveira Amorim ◽  
Larissa Santos Oliveira ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Gamma Ray ◽  
Multivariate Statistical ◽  
Musa Sp ◽  
Statistical Algorithm

Identification and characterization of two bisabolene synthases from linear glandular trichomes of sunflower (Helianthus annuus L., Asteraceae)

2016 ◽  
Vol 124 ◽  
pp. 29-37 ◽  
Author(s):  
Anna-Katharina Aschenbrenner ◽  
Moonhyuk Kwon ◽  
Jürgen Conrad ◽  
Dae-Kyun Ro ◽  
Otmar Spring
Keyword(s):  
Helianthus Annuus ◽  
Glandular Trichomes ◽  
Helianthus Annuus L ◽  
Identification And Characterization

Taxonomic characterization of members of the genus Corallococcus: Molecular divergence versus phenotypic coherency

2007 ◽  
Vol 30 (2) ◽  
pp. 109-118 ◽  
Author(s):  
Erko Stackebrandt ◽  
Orsola Päuker ◽  
Ulrike Steiner ◽  
Peter Schumann ◽  
Bettina Sträubler ◽  
...  
Keyword(s):  
Molecular Divergence ◽  
Taxonomic Characterization
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