Identification and characterization of two bisabolene synthases from linear glandular trichomes of sunflower (Helianthus annuus L., Asteraceae)

2016 ◽  
Vol 124 ◽  
pp. 29-37 ◽  
Author(s):  
Anna-Katharina Aschenbrenner ◽  
Moonhyuk Kwon ◽  
Jürgen Conrad ◽  
Dae-Kyun Ro ◽  
Otmar Spring
2011 ◽  
Vol 8 (1) ◽  
pp. 27-38 ◽  
Author(s):  
Salwa N. Zein ◽  
Abd El-khalik ◽  
Khatab Samaa ◽  
A.A.H. Eman ◽  
Clara R. Azzam

2009 ◽  
Vol 70 (9) ◽  
pp. 1117-1122 ◽  
Author(s):  
M. Adrián Troncoso-Ponce ◽  
Nicholas J. Kruger ◽  
George Ratcliffe ◽  
Rafael Garcés ◽  
Enrique Martínez-Force

2021 ◽  
Vol 2 (10) ◽  
pp. 1018-1034
Author(s):  
Osuntokun OT ◽  
Azuh VO ◽  
Adejoro BF ◽  
Akele EO

The aim of this study is to comparative study between conventional and molecular assay of isolation, identification and characterization of non-leguminous endophytic bacteria and fungi in the leguminous root samples. The plant root samples, Helianthus annuus, Carica papaya and Lycoperesicum solanum (Sunflower root and stem, pawpaw root and stem, and tomato root and stem from Adekunle Ajasin University School farm, Akungba Akoko, Ondo state, Nigeria. The isolation of endophytic bacteria were performed using the conventional method of isolation (biochemical test) and characterization were done using both the conventional and molecular method of bacteria characterization. The antibiotic susceptibility test (Antibiogram) was observed using disc diffusion. The four bacteria identified were Bacillus cereus, Enterobacter sp. Actnomycoses sp. and Aeromonas sp. for conventional method and Fusarium solani, Fusarium vortecelium and Bacillus thuringiensis for molecular method as confirmatory point of view. In this study, all isolated organisms tends to be Gram positive using the gram staining technique. Antibiogram shows the zones of inhibition with diameter ranging from 0-20 mm, Enterobacter sp. were more sensitive to the various antibiotics used. Ultraviolet spectrophotometer was also used to determine the growth dynamic as well as the death rate of the isolates, the addition of antibiotics (ciprofloxacin) to the isolates at the 24th hour speed up the death rate of the isolates from non-leguminous endophytic bacteria. After the preliminary identification of the bacteria isolates and the confirmatory identification of both bacteria and fungi isolates of the non-leguminous endophytic microorganism, it was noted that the preliminary identification was only able to achieve the genus level of taxonomic characterization, While the molecular method confirm the molecular sub level identification of isolates depletes the absolute taxonomic identification and characterization to the sub-species level. The results of this study validates the use of molecular sequencing for the assay identification and characterization of non-leguminous endophytic bacteria and fungi as the easy and best mode of identification of both bacteria and fungi isolates as a veritable tools for research purposes.


LWT ◽  
2012 ◽  
Vol 45 (1) ◽  
pp. 65-72 ◽  
Author(s):  
Pablo R. Salgado ◽  
Silvina R. Drago ◽  
Sara E. Molina Ortiz ◽  
Silvana Petruccelli ◽  
Oscar Andrich ◽  
...  

2010 ◽  
Vol 29 (3) ◽  
pp. 239-248 ◽  
Author(s):  
Diego Zavallo ◽  
Marisa Lopez Bilbao ◽  
H. Esteban Hopp ◽  
Ruth Heinz

Plant Science ◽  
2018 ◽  
Vol 272 ◽  
pp. 117-130 ◽  
Author(s):  
M.A. Troncoso-Ponce ◽  
J. Rivoal ◽  
S. Dorion ◽  
R. Sánchez ◽  
M. Venegas-Calerón ◽  
...  

Plants ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 28 ◽  
Author(s):  
Surendra Neupane ◽  
Sarah Schweitzer ◽  
Achal Neupane ◽  
Ethan Andersen ◽  
Anne Fennell ◽  
...  

Mitogen-Activated Protein Kinase (MAPK) genes encode proteins that regulate biotic and abiotic stresses in plants through signaling cascades comprised of three major subfamilies: MAP Kinase (MPK), MAPK Kinase (MKK), and MAPKK Kinase (MKKK). The main objectives of this research were to conduct genome-wide identification of MAPK genes in Helianthus annuus and examine functional divergence of these genes in relation to those in nine other plant species (Amborella trichopoda, Aquilegia coerulea, Arabidopsis thaliana, Daucus carota, Glycine max, Oryza sativa, Solanum lycopersicum, Sphagnum fallax, and Vitis vinifera), representing diverse taxonomic groups of the Plant Kingdom. A Hidden Markov Model (HMM) profile of the MAPK genes utilized reference sequences from A. thaliana and G. max, yielding a total of 96 MPKs and 37 MKKs in the genomes of A. trichopoda, A. coerulea, C. reinhardtii, D. carota, H. annuus, S. lycopersicum, and S. fallax. Among them, 28 MPKs and eight MKKs were confirmed in H. annuus. Phylogenetic analyses revealed four clades within each subfamily. Transcriptomic analyses showed that at least 19 HaMPK and seven HaMKK genes were induced in response to salicylic acid (SA), sodium chloride (NaCl), and polyethylene glycol (Peg) in leaves and roots. Of the seven published sunflower microRNAs, five microRNA families are involved in targeting eight MPKs. Additionally, we discussed the need for using MAP Kinase nomenclature guidelines across plant species. Our identification and characterization of MAP Kinase genes would have implications in sunflower crop improvement, and in advancing our knowledge of the diversity and evolution of MAPK genes in the Plant Kingdom.


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