scholarly journals Development and effect of a Lactobacillus plantarum inoculant on quality of maize grain silage

2020 ◽  
Vol 36 (2) ◽  
pp. 239-250
Author(s):  
Snezana Djordjevic ◽  
Violeta Mandic ◽  
Nikola Djordjevic
Keyword(s):  
Phylogenetic Analysis ◽  
Lactic Acid ◽  
Nutritional Value ◽  
Positive Control ◽  
Negative Control ◽  
High Moisture ◽  
Acid Detergent Fibre ◽  
Maize Grain ◽  
Competitive Product

The main aim of these studies was the characterisation and identification of lactic acid (LAB) bacteria isolated from untreated silage, and the effect of selected bacteria (inoculant was called Silko for maize) on ensiling of maize high-moisture grain. Four isolates of L(L1, L2, L3 and L4) were characterised by the use of phenotypic assays and identified by phylogenetic analysis of 16S rRNA as L. plantarum. The fresh maize high-moisture grain was ensiled with a Silko for maize inoculant, inoculant available in the market (positive control) and no additive (untreated; negative control). After 60 days of ensiling, the results showed that the chemical composition and fermentation characteristics were better in treated silages with inoculants compared to the negative control. The contents of ash, fat and lactic acid (LA) were significantly higher in the silages treated with inoculants than in negative control. In comparison, the contents of cellulose, acid detergent fibre (ADF), neutral detergent fibre (NDF), NH3-N/total nitrogen and butyric acids (BA) were considerably lower in silage treated with Silko for maize compared to the positive control. The Silko for maize improve nutritional value and fermentation of maize grain silage and is a competitive product on the market.

scholarly journals Effect of lactic acid bacteria on Listeria monocytogenes infection and innate immunity in rabbits

2020 ◽  
Vol 65 (No. 1) ◽  
pp. 23-30 ◽  
Author(s):  
Heping Zhao ◽  
Feike Zhang ◽  
Jun Chai ◽  
Jianping Wang
Keyword(s):  
Lactic Acid ◽  
Listeria Monocytogenes ◽  
Lactic Acid Bacteria ◽  
Positive Control ◽  
Negative Control ◽  
Listeriolysin O ◽  
Serum Iga ◽  
Probiotic Lactic Acid Bacteria ◽  
Spleen And Lymph Nodes ◽  
Proinflammatory Factors

The present study aimed to investigate the effect of probiotic lactic acid bacteria (LAB) addition on Listeria monocytogenes translocation and its toxin listeriolysin O (LLO), proinflammatory factors, immune organ indexes and serum immunoglobulins in farmed rabbits. Five treatments included negative control (NC), positive control (PC) with L. monocytogenes infection and supplemental LAB at 3.0 × 10<sup>6 </sup>(low-LAB, L-LAB), 3.0 × 10<sup>8</sup> (medium-LAB, M-LAB) and 3.0 × 10<sup>10 </sup>(high-LAB, H-LAB) CFU/kg of diet, respectively. The LAB was a mixture of equal amounts of Lactobacillus acidophilus (ACCC11073), Lactobacillus plantarum (CICC21863) and Enterococcus faecium (CICC20430). A total of 180 weaned rabbits (negative for L. monocytogenes) were randomly assigned to 5 groups with 6 replicates of 6 rabbits each in response to the 5 treatments. L. monocytogenes infection occurred on the first day of feeding trial and dietary LAB supplementation lasted for 14 days. The results showed that on days 7 and 14 post administration, L. monocytogenes in caecum, liver, spleen and lymph nodes was reduced in M-LAB and H-LAB compared to PC (P &lt; 0.05), and linear and quadratic reducing trends were found in liver on day 7 (P ≤ 0.002). On day 14, mucosa LLO mRNA expression and serum TNFα, IL1β and IFNγ were reduced in the three LAB treatments (P &lt; 0.05), and linear and quadratic trends were found on TNFα and IL1β (P ≤ 0.025); indexes of thymus and spleen, serum IgA and IgG were increased in the LAB treatments (P &lt; 0.05). It is concluded that LAB can be used to alleviate L. monocytogenes infection and to improve the immune function of farmed animals.

scholarly journals PSVI-1 Improvement of gut morphology and meat quality of broiler chickens by encapsulated citral and cinnamaldehyde

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 195-195
Author(s):  
Chongwu Yang ◽  
Janghan Choi ◽  
Marion Mogire ◽  
Argenis Rodas-González ◽  
Moussa S Diarra ◽  
...  
Keyword(s):  
Growth Performance ◽  
Meat Quality ◽  
Ph Value ◽  
Basal Diet ◽  
Positive Control ◽  
Negative Control ◽  
Intestinal Morphology ◽  
Post Mortem ◽  
Gut Morphology

Abstract This study investigated the effects of encapsulated citral (CIT) and cinnamaldehyde (CIN) on growth performance, intestinal morphology and meat quality in broilers. A total of 320 0-day-age male broilers (Cobb 500) were allocated 32 pens with 8 pens per treatment. The treatments included: 1) Corn-wheat-SBM basal diet (negative control); 2) basal diet with 30 ppm avilamycin premix (positive control); 3) basal diet with 50 ppm encapsulated CIT and CIN (CCL); 4) basal diet with 100 ppm encapsulated CIT and CIN (CCH). There were no significant differences between treatments in growth performance (P &gt; 0.05) at each feeding stage. Additionally, birds had similar relative organ weights of heart, liver, spleen and bursa (P &gt; 0.05). The higher ratios of villus height to crypt depth (VCR; P ≤ 0.05) were detected in broilers fed either CCL or CCH, with an average being 14.67 and 15.13 in the duodenum, and 15.13 and 13.58 in the jejunum, respectively. Regarding meat quality, a higher pH value (pH = 6.21) in the breast was observed in 24-h post mortem chickens fed CCL (P ≤ 0.05). No significant differences of pH were detected in the breast in 96-h post mortem birds. The breast color (redness, yellowness, and lightness) in either 24-h or 96-h post mortem chickens was not significantly different among treatments (P &gt; 0.05). No change in purge loss (%) was observed in the breast after 48 h. The severity of white striping (WS) or woody meat (WB) was higher in the positive control (WS = 0.58; WB = 0.15) than in other treatments (P ≤ 0.05). In conclusion, dietary treatment with encapsulated CIT and CIN improved gut morphology and meat quality of broilers by increasing VCR in the small intestine and reducing the severity of WS/WB in the breast, respectively, without affecting growth performance.

Protective Effect of 4% Titanium Tetrafluoride Varnish on Dentin Demineralization Using a Microcosm Biofilm Model

2019 ◽  
Vol 53 (5) ◽  
pp. 576-583 ◽  
Author(s):  
Daiana Moreli Soares dos Santos ◽  
Juliana Gonçalves Pires ◽  
Aline Braga Silva ◽  
Priscila Maria Aranda Salomão ◽  
Marília Afonso Rabelo Buzalaf ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Protective Effect ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Extracellular Polysaccharide ◽  
Human Saliva ◽  
Positive Control ◽  
Negative Control ◽  
Titanium Tetrafluoride ◽  
Biofilm Model

This study evaluated the effect of titanium tetrafluoride (TiF4) varnish on the development of dentin carious lesions. Bovine root dentin samples were treated for 6 h with: (A) 4% TiF4 varnish (2.45% F); (B) 5.42% sodium fluoride (NaF) varnish (2.45% F); (C) 2% chlorhexidine (CHX) gel – positive control; (D) placebo varnish; or (E) untreated – negative control (n = 4 × biological triplicate, n = 12). Treated dentin samples were exposed to human saliva mixed with McBain saliva (1:50) for the first 8 h in 24-well plates. Thereafter, the medium was removed, and McBain saliva containing 0.2% sucrose was applied for 16 h. From days 2 to 5, McBain saliva with sucrose was replaced daily (37°C, 5% CO2). The demineralization was measured using transverse microradiography, while the effect on biofilm was analyzed using viability, extracellular polysaccharide (EPS), and lactic acid production assays. The data were statistically analyzed (p < 0.05). All treatments (fluorides and CHX) significantly reduced the biofilm viability compared to placebo varnish and negative control. However, none of them was able to reduce the colony-forming unit counting for total microorganism, total streptococci, and Streptococcus mutans. NaF significantly reduced the number of Lactobacillus sp. compared to negative control. No effect was seen on lactic acid production neither on EPS synthesis, except that CHX significantly reduced the amount of insoluble EPS. Both fluorides were able to reduce dentin demineralization compared to placebo varnish and negative control; TiF4 had a better effect in reducing mineral loss and lesion depth than NaF. Therefore, TiF4 varnish has the best protective effect on dentin carious lesion formation using this model.

Clinical observation of yizhongshengxue capsule on the treatment of advanced gastric cancer-related anemia.

2017 ◽  
Vol 35 (15_suppl) ◽  
pp. e21696-e21696
Author(s):  
Li Hou ◽  
Jia Wang ◽  
Jing Wang ◽  
Ya Li ◽  
Xinyi Chen
Keyword(s):  
Quality Of Life ◽  
Gastric Cancer ◽  
Advanced Gastric Cancer ◽  
Positive Control ◽  
Negative Control ◽  
Before And After ◽  
Loss Of Appetite ◽  
Cancer Related Anemia ◽  
Basic Treatment

e21696 Background: Cancer-related anemia is an important factor that affects the clinical efficacy and quality of life for patients with advanced gastric cancer. This study is to observe the clinical efficacy of Yizhongshengxue Capsule, which is an herbal formula, on advanced gastric cancer-related anemia. Methods: A total of 96 eligible patients were randomized into three arms: 46 patients in the experimental arm, 26 patients in the positive control arm, and 24 patients in the negative control arm. All three arms received basic treatment of conventional medicine. Meanwhile, the experimental arm received Yizhongshengxue Capsule at the dosage of 3 capsules, 3 times daily. The positive control arm received Fufangejiao Syrup at the dosage of 20 ml, 3 times daily. The negative control arm received only basic treatment of western medicine. The course of treatment was 5 weeks. Results: After the 5-week-treatment, there was a statistically significant rise ( p<0.05) of hemoglobin(HGB) in both experimental arm and positive control arm. The negative control arm had no statistically difference of HGB ( p>0.05) before and after the treatment. Symptoms of dizziness, fatigue, loss of appetite, palpitation and insomnia in experimental arm were improved after the treatment ( p<0.05). Symptoms of fatigue and loss of appetite in positive control arm were alleviated after the treatment ( p<0.05). The negative control arm had no statistically difference in the above symptoms ( p>0.05) before and after the treatment. Furthermore, there was a significant improvement of dizziness, loss of appetite, palpitation and insomnia in the experimental arm compared to the negative control arm ( p<0.05). Finally, an improvement of the Karnofsky Performance Score(KPS) was seen only in the experimental arm after the treatment compared to that before the treatment ( p<0.05). Conclusions: Our study shows that Yizhongshengxue Capsule can not only improve the clinical symptoms and quality of life in patients with advanced gastric cancer-related anemia, but also reduce or even reverse the trend of HGB decrease. Therefore, it is very promising in treating cancer-related anemia. Clinical trial information: ChiCTR-TRC-13003604.

Influence of selenium source on the performance, feathering and meat quality of broilers

2017 ◽  
Vol 5 ◽  
Author(s):  
V. Ravindran ◽  
S. Elliott
Keyword(s):  
Meat Quality ◽  
Feed Intake ◽  
Sodium Selenite ◽  
Control Diet ◽  
Positive Control ◽  
Negative Control ◽  
Breast Meat ◽  
Growing Period ◽  
Breast Meat Yield

SummaryThe following trial was conducted to investigate the effects of Se source (inorganic sodium selenite (SS) versus organic use Sel-Plex® (SP; Alltech Inc, Nicholasville, KY, USA)) on the performance, feathering and meat quality of 900, one-day-old male broilers over a 42 day growing period. Pens (n = 20) were randomly assigned to either: Diet A (no Se; negative control), Diet B as for diet A plus 0.3 mg/kg SS (positive control), Diet C as for diet A plus 0.2 mg/kg SP, Diet D as for diet A plus 0.3 mg/kg SP and Diet E as for diet A plus 0.4 mg/kg SP. From day 1 to 28, significant (P < 0.05) treatment effects were seen for feed intake and FCR, with values for the negative control being higher than for the diets containing Se. The best FCR was recorded for the diet containing 0.4 mg/kg SP. Breast meat yield for birds fed the negative control were lower than those fed selenite or SP supplemented diets (P < 0.05). Feather measurements on d 14, 28 and 42 of age showed that, although scores became poorer with age (4.9 at 14 d, 4.6 at 28 d and 3.6 at 42 d), they were unaffected (P > 0.05) by dietary Se inclusion. Numeric trends in favour of SP were seen in cooking losses in meat chilled for 24 hours and seven days. Cooking losses in meat frozen for seven days were significantly influenced by Se inclusion, whereby values for birds fed 0.3 and 0.4 mg/kg SP were significantly lower than values for birds fed inorganic selenite (P < 0.05). Tissue Se concentrations were highest for the SP diets. Meat oxidation from the birds fed SP was lower than for the birds fed no Se or SS. The results demonstrated that SP may be more advantageous in improving certain variables of performance and meat quality compared to inorganic sources of Se.

scholarly journals Influence of species and preservations on the quality and safety of grass silages

2010 ◽  
Vol 58 (5) ◽  
pp. 329-336
Author(s):  
Jiří Skládanka ◽  
Petr Doležal ◽  
Jan Nedělník ◽  
Hana Moravcová ◽  
Roman Poštulka ◽  
...  
Keyword(s):  
Organic Matter ◽  
Lactic Acid ◽  
Lolium Perenne ◽  
Water Extract ◽  
Grass Species ◽  
Crude Fibre ◽  
Acid Detergent Fibre ◽  
Organic Nutrients ◽  
Biological Additive

The paper evaluates the quality of model silages made of wilted grass biomass and treated with silage additives. Grass species used for the production of silages were Lolium perenne, Festulolium pabulare and Festulolium braunii harvested in the first cut at the stage of earing. The assessed grass species were wilted after the cut for an identical time 36 hours (2008), resp. 24 hours (2009). The treatment was made either with a chemical preparation (formic acid, propionic acid, ammonium formate) and/or with a bio­lo­gi­cal inoculant (Enterococcus faecium, Lactobacillus plantarum, Pediococcus acidilactici, Lactobacillus salivarius, cellulase, hemicellulase and amylase). The amount of the chemical ingredient was 4 l . t−1 and the amount of the biological additive was 10 g . t−1.The biomass was after wilting ensilaged in con­tai­ners whose diameter and height were 0.15 m and 0.64 m, respectively. After 60 days of ensilaging, the silages were assessed for pH, organic acids content, ethanol content and acidity of water extract (AWE); organic nutrients assessed in the silages were crude fibre (CF), neutral detergent fibre (NDF), acid detergent fibre (ADF), crude protein (CP) and digestibility of organic matter (DOM). Hygienic safety was assessed from the contents of zearalenon, fumonisin and aflatoxin mycotoxins. The high (P < 0.05) dry matter (DM) content in Festulolium pabulare silages indicates that the species tends to rapid wilting. The higher DM content reflected in lower biomass losses (P < 0.05). The lowest pH values (P < 0.05) were detected in silages made of Festulolium braunii. The fact relates to the higher content of lactic acid in the prepared microsilages. The use of ensiling additives affected the quality of extracts. Namely the application of the biological additive led to the increased content (P < 0.05) of not only lactic acid but acetic acid too. Titrable acidity was not affected by the ensiling additives. As to the emanation of ethanol, heterofermentative bacteria of lactic fermentation apparently took part in the fermentation process of the silages as well. While the evaluated forage species showed differences in the di­ges­ti­bi­li­ty of organic matter (P < 0.05), the application of preservatives did not influence the content of organic nutrients. Fumonisin was not detected at all and aflatoxins were below the level of detection. None of the assessed factors had an effect on the content of zearalenon; in spite of the fact, an apparent tendency towards a higher content of zearalenon was recorded in Lolium perenne.

scholarly journals Gambaran Daya Terima Minuman Sari Buah Pedada (Sonneratia sp.) dengan Penambahan Gula Stevia (Stevia rebaudiana)

2021 ◽  
Vol 10 (2) ◽  
pp. 89-99
Author(s):  
Dini W Dari ◽  
Sri Rahmadhani ◽  
Dini Junita
Keyword(s):  
Nutritional Value ◽  
Fruit Juice ◽  
Descriptive Analysis ◽  
Stevia Rebaudiana ◽  
Positive Control ◽  
Negative Control ◽  
Acceptance Testing ◽  
Acceptance Test ◽  
Coastal Plant ◽  
Exponential Method

One type of coastal plant in the mangrove forest is the pedada (Sonneratia sp.). This fruit has high nutritional value and has the potential to be processed into food products. This fruit is still rarely used because of its sour taste. One form of food processed from pedada fruit is pedada juice drink. The purpose of this study was to describe the acceptance of the parameters of color, aroma, texture, taste, and overall acceptance in the form of selecting the best formulation, which was analyzed using the exponential method of pedada fruit juice drinks. A descriptive analysis within the experimental design was applied in this research. There were 5 samples in this study, namely A1 positive control (200 g, 70% sucrose sugar), A2 negative control (200 g pedada, 0% stevia sugar), A3 (200 g pedada, 9% stevia sugar), A4 (pedada 200 g, 18% stevia sugar), and A5 (200 g pedada, 36% stevia sugar). The acceptance test used the hedonic method with 40 consumer panelists aged 17-35 years. This research was conducted in May-August 2020. The manufacture of pedada fruit juice drinks and acceptance testing were carried out at home. The results showed that the A4 sample of 200 g of pedada fruit with the addition of 18% stevia sugar obtained the highest score on the acceptability of pedada fruit juice drinks and overall acceptance. The average results of each color, aroma, texture, and taste acceptability parameter were 3.9, 3.9, 4.0, and 4.0, respectively. Keywords: Acceptance; fruit juice; pedada; stevia sugar   ABSTRAK Indonesia kaya akan hasil alamnya seperti berbagai jenis hutan yang salah satunya adalah hutan mangrove. Hutan mangrove itu sendiri memiliki berbagai jenis tumbuhan pantai seperti pedada atau Sonneratia sp. Buah ini memiliki nilai gizi yang tinggi dan berpotensi untuk diolah menjadi produk pangan. Buah ini masih jarang digunakan karena rasanya yang asam. Salah satu bentuk pengolahan makanan dari buah pedada adalah minuman sari buah pedada. Adapun tujuan dari penelitian ini yaitu untuk mendeskripsikan penerimaan terhadap parameter warna, aroma, tekstur, dan rasa serta penerimaan secara keseluruhan berupa pemilihan formulasi terbaik yang dianalisis menggunakan metode eksponensial minuman sari buah pedada. Penelitian ini merupakan penelitian eksperimental dengan desain analisis deskriptif murni. Sampel dalam penelitian ini sebanyak 5 sampel yaitu kontrol positif A1 (200 g, gula sukrosa 70%), kontrol negatif A2 (200 g pedada, gula stevia 0%), A3 (200 g pedada, gula stevia 9%), A4 ( pedada 200 g, gula stevia 18%), A5 (200 g pedada, gula stevia 36%). Uji penerimaan menggunakan metode hedonis dengan 40 panelis konsumen berusia 17-35 tahun. Penelitian ini dilakukan pada bulan Mei-Agustus 2020, pembuatan minuman sari buah pedada dan uji daya terima dilakukan di rumah. Akseptabilitas minuman sari buah pedada dan penerimaan keseluruhan yaitu sampel minuman A4 buah pedada 200 g dengan penambahan gula stevia 18%, diperoleh skor rata-rata tertinggi (4,0) dengan hasil rata-rata masing-masing parameter akseptabilitas warna 3.9, aroma 3.9, tekstur 4.0, dan rasa 4.0. Kata Kunci: Daya terima, gula stevia, pedada, sari buah

scholarly journals Microbiological Quality and Proximate Composition of Bakasang Laor, a Traditional Fermented Fishery Product in Maluku

2020 ◽  
Vol 12 (1) ◽  
pp. 64-69
Author(s):  
Ferymon Mahulette ◽  
Tri Santi Kurnia
Keyword(s):  
Lactic Acid ◽  
Proximate Composition ◽  
Nutritional Value ◽  
Microbiological Quality ◽  
Raw Material ◽  
Coliform Bacteria ◽  
Fishery Products ◽  
Microbiological Research ◽  
Better Than

Bakasang laor is one of the traditional fermented fishery products in Maluku, Indonesia. There are two types of  bakasang laor, i.e. with and without vinegar. The microbiological research of bakasang processed use laor as raw material  has never been done before. The research aimed to analyze the microbiological quality and proximate composition of two types of bakasang laor.  The proximate composition can determine the nutritional value of  this product for consumption by the community. The sample of bakasang laor was taken from traditional producer in Latuhalat village, Ambon. The total number of halotolerant and coliform bacteria  in bakasang laor without vinegar were 1.7x106 CFU/g and 4.1x106 CFU/g, respectively, while the total of lactic acid bacteria in bakasang laor with vinegar was 6.0x106 CFU/g.  The carbohydrate, protein, fat and ash contents of bakasang laor with vinegar was higher than bakasang laor without vinegar. The carbohydrate, protein and fat contents of bakasang laor with vinegar were 19.64 %, 16.23 %, and 2.42 %, respectively. Generally, microbiological quality and proximate composition  of  bakasang laor with vinegar was better than bakasang laor without  vinegar. The bacteria that play a role in fermentation can be used as a starter in the fermentation of various food products.  The results of this research to improve the quality of this fermentation product in the future.

scholarly journals EFEKTIVITAS BAKTERI ASAM LAKTAT YANG DIISOLASI DARI SUSU KUDA SUMBA TERHADAP KUALITAS SILASE JERAMI PADI

2019 ◽  
Vol 6 (1) ◽  
pp. 31-37
Author(s):  
Annytha Ina Rohi Detha ◽  
Frans Umbu Datta ◽  
Elisabet Beribe ◽  
Nancy D. F. K. Foeh ◽  
Nemay Ndaong
Keyword(s):  
Lactic Acid ◽  
Treatment Group ◽  
Lactic Acid Bacteria ◽  
Rice Straw ◽  
Positive Control ◽  
Positive Control Group ◽  
Control Group ◽  
Golden Color

This study aims to evaluate the ability of lactic acid bacteria isolated from Sumba horse milk as a starter in improving the quality of rice straw silage. Determination of the quality of rice straw silage through organoleptic tests, measurement of percentage of silage damage and silage pH. there are 7 treatments based on silage compositions and lactic acid bacteria. The results showed the presence of golden color in all treatments as the original color, the texture was very soft to soft and not slimy, and the presence of a distinctive silage odor or close to the typical smell of silage in all treatments. The presentation of silage damage to rice straw in the positive control group showed the highest percentage of damage that was equal to 43.58%, while the percentage of damage in the treatment group ranged from 28.57% to 41.02%. The pH results showed the control group had a pH of 3.6 and the P1-P6 group had an average pH ranging from 5.7 to 6.5. The addition of lactic acid bacteria in the manufacture of silage in rice straw provides good quality silage results.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK BIJI BUAH DUKU Lansium domesticum TERHADAP BAKTERI STAPHYLOCOCUS AUREUS DAN ESCHERICHIA COLI

PHARMACON ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 818
Author(s):  
Albrita Pehino ◽  
Fatimawali Fatimawali ◽  
Elly J. Suoth
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Nutritional Value ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Activity Test ◽  
Solution Studies ◽  
Lansium Domesticum

ABSTRACT Duku (Lansium domesticum) provides many benefits for the community. Apart from the fruit which has high nutritional value, duku is believed by the community to have benefits. The study aims to learn about the antibacterial extract of my seed against the staphylococus aureus and escherichia coli. This study used the meseration method with ethanol as a solvent. The antibacterial activity test used the well diffusion method with a concentration of 10%, 20%, 30%, 40%. The positive control used was Ciprofloxacin and the negative control used was the CMC solution. Studies show that the suppression of extract is 10%, 20%, 30%, and 40% staphylococus aureus, the average drip zone is 11.3mm, 13.3mm, 13.6mm, 11,3mm, and escherichia coli, on average, 10 mm,  9,3mm, 10,3mm, 12,6 mm. The results suggest that the extract of the duku fruit has antibacterial activity against the growth of the staphylococcus aureus and escherichia coli. Keywords: Duku seed, Antibacterial, Antibacterial activity ABSTRAK Duku (Lansium domesticum) banyak memberikan manfaat bagi masyarakat. Selain buahnya yang mempunyai nilai gizi tinggi, duku dipercaya masyarakat memiliki manfaat untuk kesehatan. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak biji duku terhadap bakteri staphylococus aureus  dan Escherichia coli. Penelitian ini menggunakan metode maserasi dengan pelarut etanol. Uji aktivitas antibakteri menggunakan metode difusi sumuran  dengan konsentrasi 10%, 20%, 30%, 40%. Kontrol positif yang digunakan adalah  Ciprofloxacin dan kontrol negatif yang digunakan adalah larutan CMC.  Hasil penelitian menunjukan bahwa daya hambat ekstrak dengan konsentrasi 10%, 20%, 30%, dan 40% Staphylococcus aureus rata-rata  zona beningnya 11,3mm, 13,3mm, 13,6mm, 11,3mm, dan Escherichia coli rata-rata  zona beningnya 10 mm, 9,3mm, 10,3mm, 12,6 mm. Dari hasil yang didapatkan  disimpulkan bahwa ekstrak biji buah duku memiliki aktivitas antibakteri terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli. Kata Kunci:Biji buah duku, Antibakteri, Aktivitas antibakteri

Sign in / Sign up

Export Citation Format

Share Document