scholarly journals Solid-phase extraction of antioxidant compounds from commercial cranberry extract and its antiradical activity

2007 ◽  
pp. 157-164 ◽  
Author(s):  
Vesna Tumbas ◽  
Sonja Djilas ◽  
Jasna Canadanovic-Brunet ◽  
Gordana Cetkovic ◽  
Sladjana Savatovic
Keyword(s):  
Vitamin C ◽  
Solid Phase Extraction ◽  
Solid Phase ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Content ◽  
Antioxidant Compounds ◽  
Phase Extraction ◽  
Spin Resonance

This study is concerned with the fractionation and determination of major antioxidant compounds (phenols, flavonoids, anthocyanins and vitamin C) in commercial cranberry extract. The total content of phenolics, flavonoids and total and monomers of anthocyanins, determined spectrophotometrically, was 1.67 mg/g, 0.41 mg/g, 5.12 mg/g and 3.32 mg/g. The content of vitamin C, determined volumetrically, was 121.74 mg/g. Commercial cranberry extract was dissolved in 80 % acetone and the solution was fractionated using solid phase extraction (SPE) in order to abstract vitamin C, neutral and acidic phenols. The free radical scavenging activity of the cranberry extract and its fractions was investigated on stable 2,2-diphenyl-1-picrylhydrazyl (DPPH) and reactive hydroxyl radicals employing electron spin resonance (ESR) spectroscopy. The most effective fractions were those containing vitamin C (AADPPH= AAOH=100%), neutral (AADPPH=89.50% and AAOH=43.11%) and acidic (AADPPH=83.98% and AAOH=38.58%) phenols. The presence of vitamin C, abstracted from cranberry extract, was determined by Fe(III)-mediated ascorbate oxidation which yields characteristic ESR doublet spectrum of ascorbyl radical.

scholarly journals Bioactivity of extracts of Centaurea polyclada dc. (Asteraceae)

2009 ◽  
Vol 61 (3) ◽  
pp. 447-452 ◽  
Author(s):  
Melanie Granger ◽  
Emilie Samson ◽  
Severine Sauvage ◽  
Anisha Majumdar ◽  
Poonam Nigam ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Free Radical ◽  
Solid Phase Extraction ◽  
Brine Shrimp ◽  
Solid Phase ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Phase Extraction ◽  
Brine Shrimp Lethality Assay

The free radical-scavenging property, antibacterial activity, and brine shrimp toxicity of n-hexane, dichloromethane (DCM), and methanol (MeOH) extracts of Centaurea polyclada, an endemic Turkish species, were assessed using the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay, the resazurin microtiter plate-based assay, and the brine shrimp lethality assay, respectively. The DCM and MeOH extracts of C. polyclada exhibited free radical-scavenging ability with RC50 values 1.17 and 0.015 mg/mL, respectively. Among solid-phase extraction fractions of the MeOH extract, the fraction eluted with 60% MeOH in water demonstrated the highest level of free radical-scavenging activity (RC50 = 0.016 mg/mL). Only the DCM extract showed considerable antibacterial activity against all nine test strains except Escherichia coli, with MIC ranging from 1.25 to 2.50 mg/mL. This antibacterial activity pattern was also observed with solid-phase extraction fractions of the DCM extract with varied potencies. None of the extracts showed any significant toxicity towards brine shrimps (LD50 = >1.00 mg/mL).

Determination of the total content of some sulfonamides in milk using solid-phase extraction coupled with off-line derivatization and spectrophotometric detection

2015 ◽  
Vol 188 ◽  
pp. 51-56 ◽  
Author(s):  
Stanislava G. Dmitrienko ◽  
Elena V. Kochuk ◽  
Veronika V. Tolmacheva ◽  
Vladimir V. Apyari ◽  
Yury A. Zolotov
Keyword(s):  
Solid Phase Extraction ◽  
Solid Phase ◽  
Total Content ◽  
Phase Extraction ◽  
Spectrophotometric Detection
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