scholarly journals Retention behaviour of estradiol derivatives in reversed phase HPLC

2005 ◽  
pp. 169-178
Author(s):  
Marijana Acanski ◽  
Slobodan Petrovic
Keyword(s):  
Linear Relationship ◽  
Silica Gel ◽  
Mobile Phase ◽  
Volume Fraction ◽  
Reversed Phase ◽  
Considerable Effect ◽  
Reversed Phase Hplc ◽  
Retention Behaviour ◽  
Estradiol Derivatives ◽  
Octadecyl Silica Gel

Retention behaviour ofestradiol derivatives has been studied by the reversed phase HPLC. LiChrosorb RP-18 column end eluents consisting of methanol respectively aceto-nitrile and water in various proportions, were used. It was shown that there is a linear relationship between the retention constant and the volume fraction of organic component in the mobile phase. The slopes and intercepts of lines are in agreement with the polarity of derivatives studied. Considerable effect on the chromatographic behaviour exerted the type and position of substituents in the molecule of estradiol derivative. Results obtained in these investigations are compared with results of the same derivatives obtained in previous investigations on octadecyl silica gel in TLC with eluent methanol-water in various proportions.

The liquid chromatography of cholesterol esters

1991 ◽  
Vol 56 (4) ◽  
pp. 796-808 ◽  
Author(s):  
Ladislav Svoboda ◽  
Alena Krejčová
Keyword(s):  
Liquid Chromatography ◽  
Silica Gel ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Cholesterol Esters ◽  
Octadecyl Silica Gel ◽  
Unsaturated Carboxylic Acids ◽  
Retention Characteristics ◽  
Phase Systems

The retention characteristics were found for cholesterol and 19 of its esters with saturated and unsaturated carboxylic acids containing 1 to 22 carbon atoms using high performance liquid chromatography in the normal and reversed-phase systems. HPLC on silica gel in a mobile phase consisting of heptane and 2-propanol or ethyl acetate is especially suitable for analysis of the esters of the lower acids, while chromatography on an octadecyl silica gel sorbent in a water-tetrahydrofuran or acetonitrile-dichlormethane mobile phase can bee successfully used for the separation of esters with acids containing 10–22 carbon atoms, that can differ in the presence of double bonds in the acyl or through cis-trans isomerism.

scholarly journals Simultaneous Determination of Preservatives (Methyl Paraben and Propyl Paraben) in Sucralfate Suspension Using High Performance Liquid Chromatography

2011 ◽  
Vol 8 (1) ◽  
pp. 340-346 ◽  
Author(s):  
Rajesh M. Kashid ◽  
Santosh G. Singh ◽  
Shrawan Singh
Keyword(s):  
High Performance Liquid Chromatography ◽  
Simultaneous Determination ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Hplc Method ◽  
Reversed Phase Hplc ◽  
Methyl Paraben ◽  
Propyl Paraben

A reversed phase HPLC method that allows the separation and simultaneous determination of the preservatives methyl paraben (M.P.) and propyl paraben (P.P.) is described. The separations were effected by using an initial mobile phase of water: acetonitrile (50:50) on Inertsil C18 to elute P.P. and M.P. The detector wavelength was set at 205 nm. Under these conditions, separation of the two components was achieved in less than 10 min. Analytical characteristics of the separation such as precision, specificity, linear range and reproducibility were evaluated. The developed method was applied for the determination of preservative M.P. and P.P. at concentration of 0.01 mg/mL and 0.1 mg/mL respectively. The method was successfully used for determining both compounds in sucralfate suspension.

Retention behaviour of crown ethers and actinomycin D in reversed-phase HPLC

1992 ◽  
Vol 34 (3-4) ◽  
pp. 155-158 ◽  
Author(s):  
A. Horti ◽  
E. Glibin ◽  
V. Nesterov
Keyword(s):  
Crown Ethers ◽  
Actinomycin D ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Retention Behaviour

scholarly journals Correlation between retention and C-18 silica gel surface coverage in reversed-phase liquid chromatography

2000 ◽  
Vol 65 (11) ◽  
pp. 811-818
Author(s):  
Marijana Acanski ◽  
Slobodan Petrovic ◽  
Vjera Pejanovic ◽  
Julijana Petrovic
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Carbon Content ◽  
Silica Gel ◽  
High Performance ◽  
Surface Coverage ◽  
Reversed Phase ◽  
Reversed Phase Liquid Chromatography ◽  
Retention Behaviour ◽  
Phase Liquid

The effect of C-18 silica gel surface coverage on the retention behaviour of some estrone derivatives in reversed phase high performance liquid chromatography has been studied. Two commercial columns with different C-18 coverage, Spherisorb ODS-1(8 % carbon content) and Li Chrosorb RP-18(22% carbon content), using methanol-water as the eluent, were used.

scholarly journals Retention behaviour of acyclic polyfunctional D-arabinose derivatives

2002 ◽  
pp. 35-44 ◽  
Author(s):  
Ljiljana Kolarov ◽  
Eva Loncar ◽  
Radomir Malbasa
Keyword(s):  
Mobile Phase ◽  
Volume Fraction ◽  
Retention Mechanism ◽  
Biologically Active ◽  
Polar Component ◽  
Chiral Molecules ◽  
Retention Behaviour ◽  
Polar Components ◽  
Solute Retention ◽  
Mobile Phases

It is well known that some of biologically active compounds are chiral molecules. Natural monosaccarides are applicable as chiral precursors. D-arabinose is an appropriate chiral precursor in the synthesis of very important biomolecules such as biotin and its analogues and feromon )-exobrevicomin. Acyclic poly functional D- arabinose derivatives are intermediates in the synthesis of the former biomolecules. This paper deals with the retention behaviour and retention mechanism of acyclic poly functional D-arabinose derivatives. The tested compounds have been chromato graphed on silica gel thin layer using binary organic mobile phases with a different polarity. With increasing polarity of the eluent polar components the solute retention is decreasing. The linear relationship is obtained between the values of retention constant, RM' and the logarithm of the mobile phase polar component volume fraction for all solutes tested and eluents used.

Influence of Metal Impurities in Silica Gel on Protein Analysis in Reversed-phase HPLC

1990 ◽  
Vol 19 (4) ◽  
pp. 687-690 ◽  
Author(s):  
Junichi Koyama ◽  
Junko Nomura ◽  
Yutaka Ohtsu ◽  
Okitsugu Nakata ◽  
Motoji Takahashi
Keyword(s):  
Silica Gel ◽  
Protein Analysis ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Metal Impurities
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