High temperature reversed-phase HPLC using deuterium oxide as a mobile phase for the separation of model pharmaceuticals with multiple on-line spectroscopic analysis (UV, IR, 1H-NMR and MS)

The Analyst ◽  
2001 ◽  
Vol 126 (10) ◽  
pp. 1625-1629 ◽  
Author(s):  
Dave Louden ◽  
Alan Handley ◽  
Steve Taylor ◽  
Ian Sinclair ◽  
Eva Lenz ◽  
...  
Keyword(s):  
High Temperature ◽  
1H Nmr ◽  
Mobile Phase ◽  
Spectroscopic Analysis ◽  
Deuterium Oxide ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
On Line

Reversed-phase HPLC of polymer additives with multiple on-line spectroscopic analysis (UV, IR, 1H NMR and MS)

2002 ◽  
Vol 373 (6) ◽  
pp. 508-515 ◽  
Author(s):  
Dave Louden ◽  
Alan Handley ◽  
Eva Lenz ◽  
Ian Sinclair ◽  
Steve Taylor ◽  
...  
Keyword(s):  
1H Nmr ◽  
Spectroscopic Analysis ◽  
Reversed Phase ◽  
Polymer Additives ◽  
Reversed Phase Hplc ◽  
On Line

HPLC Analysis of Ecdysteroids in Plant Extracts Using Superheated Deuterium Oxide with Multiple On-Line Spectroscopic Analysis (UV, IR,1H NMR, and MS)

2002 ◽  
Vol 74 (1) ◽  
pp. 288-294 ◽  
Author(s):  
Dave Louden ◽  
Alan Handley ◽  
Rene Lafont ◽  
Steve Taylor ◽  
Ian Sinclair ◽  
...  
Keyword(s):  
1H Nmr ◽  
Plant Extracts ◽  
Hplc Analysis ◽  
Spectroscopic Analysis ◽  
Deuterium Oxide ◽  
On Line

scholarly journals Simultaneous Determination of Preservatives (Methyl Paraben and Propyl Paraben) in Sucralfate Suspension Using High Performance Liquid Chromatography

2011 ◽  
Vol 8 (1) ◽  
pp. 340-346 ◽  
Author(s):  
Rajesh M. Kashid ◽  
Santosh G. Singh ◽  
Shrawan Singh
Keyword(s):  
High Performance Liquid Chromatography ◽  
Simultaneous Determination ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Hplc Method ◽  
Reversed Phase Hplc ◽  
Methyl Paraben ◽  
Propyl Paraben

A reversed phase HPLC method that allows the separation and simultaneous determination of the preservatives methyl paraben (M.P.) and propyl paraben (P.P.) is described. The separations were effected by using an initial mobile phase of water: acetonitrile (50:50) on Inertsil C18 to elute P.P. and M.P. The detector wavelength was set at 205 nm. Under these conditions, separation of the two components was achieved in less than 10 min. Analytical characteristics of the separation such as precision, specificity, linear range and reproducibility were evaluated. The developed method was applied for the determination of preservative M.P. and P.P. at concentration of 0.01 mg/mL and 0.1 mg/mL respectively. The method was successfully used for determining both compounds in sucralfate suspension.

Flow injection analysis with multiple on-line spectroscopic analysis (UV, IR, 1H-NMR and MS)

2002 ◽  
Vol 27 (1-2) ◽  
pp. 191-200 ◽  
Author(s):  
Eva Lenz ◽  
Steve Taylor ◽  
Craig Collins ◽  
Ian D Wilson ◽  
Dave Louden ◽  
...  
Keyword(s):  
1H Nmr ◽  
Flow Injection ◽  
Flow Injection Analysis ◽  
Spectroscopic Analysis ◽  
On Line

Ionic liquids improved reversed-phase HPLC on-line coupled with ICP-MS for selenium speciation

Talanta ◽  
2011 ◽  
Vol 83 (3) ◽  
pp. 724-731 ◽  
Author(s):  
Beibei Chen ◽  
Man He ◽  
Xiangju Mao ◽  
Ran Cui ◽  
Daiwen Pang ◽  
...  
Keyword(s):  
Ionic Liquids ◽  
Reversed Phase ◽  
Selenium Speciation ◽  
Reversed Phase Hplc ◽  
Icp Ms ◽  
On Line

scholarly journals Application of a reversed-phase HPLC method for quantitative p-coumaric acid analysis in wine

OENO One ◽  
2010 ◽  
Vol 44 (2) ◽  
pp. 117
Author(s):  
Dominique Salameh ◽  
Cédric Brandam ◽  
Toufic Rizk ◽  
Roger Lteif ◽  
Pierre Strehaiano
Keyword(s):  
Mobile Phase ◽  
Chromatographic Method ◽  
Red Wine ◽  
Reversed Phase ◽  
Hplc Method ◽  
Coumaric Acid ◽  
Reversed Phase Hplc ◽  
Mobile Phase Composition ◽  
Rp Hplc ◽  
Synthetic Media

<p style="text-align: justify;"><strong>Aims</strong>: This paper presents a rapid chromatographic method to monitor the concentration of <em>p</em>-coumaric acid in wine and in bioconversion studies.</p><p style="text-align: justify;"><strong>Methods and results</strong>: RP-HPLC method was validated in synthetic wine medium and in natural red wine. Mobile phase composition was water 77%, acetonitrile 23%. Formic acid was added to control pH at 3.5. The flow was 0.7 mL/min and the temperature 30 °C. The detection was done using UV at 305 nm. The linearity range was validated between 0.5 and 15 mg/L. The resolution was respectively 5.35 and 2.99. The detection and quantification limits were 0.01 mg/L and 0.04 mg/L. This method was used to study <em>p</em>-coumaric acid bioconversion into 4-ethylphenol and 4-vinylphenol, and to study this acid adsorption in enological conditions.</p><p style="text-align: justify;"><strong>Conclusions</strong>: This paper presented a simple HPLC method to monitor the concentration of <em>p</em>-coumaric acid in synthetic media and natural wine. It was used to study the <em>p</em>-coumaric acid bioconversion rates and mechanism.</p><p style="text-align: justify;"><strong>Significance and impact of the study</strong>: This method is useful to monitor <em>p</em>-coumaric acid concentration, which helps to predict amounts of 4-ethylphenol or 4-vinylphenol that can be produced in wine. This method can be helpful to control undesirable phenolic flavors potential in wine.</p>

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