scholarly journals Endophytic bacteria of Catharanthus roseus as an alternative source of Vindoline and application of response surface methodology to enhance its production

2019 ◽  
Vol 71 (1) ◽  
pp. 27-38 ◽  
Author(s):  
Nawed Anjum ◽  
Ramesh Chandra
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Catharanthus Roseus ◽  
Endophytic Bacteria ◽  
Cost Effective ◽  
Ionization Mass ◽  
Nutritional Requirement ◽  
Alternative Source ◽  
Anticancer Compounds ◽  
Cost Efficient

The requirement for novel, cost-effective pharmaceutical compounds is growing. We screened endophytic bacteria of Catharanthus roseus for the production of vinca alkaloids. Vindoline-producing endophytic bacteria was identified as Microbacterium sp. Vindoline was purified and characterized using column chromatography, TLC, HPLC and electrospray ionization mass spectrometry. The subsequent aim of this study was to design a cost-efficient media that can maximize vindoline production from Microbacterium sp. Initial optimization of the nutritional requirement and of the process parameters were carried out by monothetic analysis. Key factors obtained were optimized by the central composite design of response surface methodology. This model was also validated by repeating the experiment under the optimized conditions, which resulted in maximum production of 82 ?g/L (predicted response was 76.21 ?g/L). Reliability of the experiments was confirmed by ANOVA analysis, regression coefficient, prediction vs actual chart and a 3D response plot, which determined the fit of the model. This study should serve as a reference point for the use of statistical techniques in bacterial systems for production of bioactive, anticancer compounds by plant endophytes.

Application of response surface methodology for optimization of growth and lipids in Scenedesmus abundans using batch culture system

RSC Advances ◽  
2014 ◽  
Vol 4 (42) ◽  
pp. 22129-22140 ◽  
Author(s):  
Chelladurai Chellamboli ◽  
Muthiah Perumalsamy
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Batch Culture ◽  
Culture System ◽  
Fossil Fuels ◽  
Biofuel Production ◽  
Alternative Source ◽  
System P

Owing to an increased demand for fuel and the depletion of fossil fuels, an alternative source such as algae is currently being exploited for biofuel production.

Optimization of Culture Conditions for Cold-Active Cellulase Production by Penicillium cordubense D28 Using Response Surface Methodology

2011 ◽  
Vol 183-185 ◽  
pp. 994-998
Author(s):  
Shuo Dong ◽  
Nai Yu Chi ◽  
Qing Fang Zhang
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Cellulase Activity ◽  
Cellulase Production ◽  
Culture Conditions ◽  
Batch Reactors ◽  
Corn Meal ◽  
Cold Active ◽  
Initial Ph ◽  
Cost Efficient

The design of an optimum and cost-efficient medium for production of cold-active cellulase by Penicillium cordubense D28 was attempted by using response surface methodology (RSM). Based on the Plackett–Burman design, corn meal, (NH4)2SO4 and branc were selected as the most critical nutrient. Subsequently, they were investigated by the Box-Behnken design. Results showed that the maximum cold-active cellulase activity of 110.4U/mL was predicted when the concentration of corn meal, (NH4)2SO4 and branc were 21.97 g/L, 2.39 g/L and 14.99 g/L, respectively. The results were further verified by triplicate experiments. The batch reactors were operated under an optimized condition of the respective corn meal, (NH4)2SO4 and branc concentration of 22 g/L , 2.4 g/L and 15 g/L , the initial pH of 6.0 and experimental temperature of 20 ± 1°C. Without further pH adjustment, the maximum cold-active cellulase activity of 109.8 U/mL was obtained based on the optimized medium with further verified the practicability of this optimum strategy.

scholarly journals Response surface methodology for optimization studies of hydro-distillation of essential oil from pixie mandarin (Citrus reticulata Blanco) peels

2021 ◽  
Vol 23 (4) ◽  
pp. 26-34
Author(s):  
Tan Phat Dao ◽  
Ngo Thi Cam Quyen ◽  
Tran Thi Yen Nhi ◽  
Chi Cuong Nguyen ◽  
Trung Thanh Nguyen ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Essential Oil ◽  
Response Surface ◽  
Target Function ◽  
Extraction Process ◽  
Coefficient Of Determination ◽  
Gas Chromatography Mass Spectrometry ◽  
P Value ◽  
Alternative Source ◽  
Mathematical Techniques

Abstract Essential oil extraction technique from mandarin pixie peels by hydro-distillation is optimized by response surface methodology (RSM). Mathematical techniques were used in experimental design to evaluate the impacts of factors that affect the extraction process and improve the yield of the extraction process. A central mixed design based on influencing variables such as water ratio (3–5 mL/g), temperature (110–130 °C) and extraction time (90–150 min) was adopted with essential oil yield as the target function. Correlation analysis of the mathematical regression model showed that the quadratic polynomial model can be used to optimize hydro-distillation of pixie mandarin oil. The results showed that under the optimum extraction conditions, the highest quantity of essential oils was achieved (7.28 mL/100 g materials). In terms of statistical analysis, the significance levels (p-value <0.05) of the model showed that the experimental results had a good impact between factors. The coefficient of determination indicating the match between the experimental value and the predicted value of the model was high (R2>0.9). The chemical composition of the essential oil was analyzed by Gas Chromatography-Mass Spectrometry, revealing the dominance of limonene content (97.667%), which implies that the essential oil of pixie mandarin could be an alternative source of limonene.

scholarly journals Optimization of Geometry Parameters of Inkjet-Printed Silver Nanoparticle Traces on PDMS Substrates Using Response Surface Methodology

Materials ◽  
2019 ◽  
Vol 12 (20) ◽  
pp. 3329 ◽  
Author(s):  
Jumana Abu-Khalaf ◽  
Loiy Al-Ghussain ◽  
Ahmad Nadi ◽  
Razan Saraireh ◽  
Abdulrahman Rabayah ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Line Width ◽  
Silver Nanoparticle ◽  
Inkjet Printing ◽  
Cost Effective ◽  
Optimal Number ◽  
Gauge Factor ◽  
Straight Line ◽  
Input Parameters

Inkjet printing is an emerging technology with key advantages that make it suitable for the fabrication of stretchable circuits. Specifically, this process is cost-effective and less complex compared to conventional fabrication technologies. Inkjet printing has several process and geometry parameters that significantly affect the electromechanical properties of the printed circuits. This study aims to optimize the geometry parameters of inkjet-printed silver nanoparticle traces on plasma-treated polydimethylsiloxane (PDMS) substrates. The optimization process was conducted for two printed shapes, namely straight line and horseshoe patterns. The examined input factors for the straight line traces were: the number of inkjet-printed layers and line width. On the other hand, the number of cycles and amplitude were the examined input parameters for the horseshoe shape. First, the optimal number of layers and line width were found from the straight line analysis and subsequently were used in the optimization of the horseshoe pattern parameters. The optimization of the input parameters was carried out using the response surface methodology (RSM), where the objective of the optimization was to maximize the breakdown strain of the traces while maximizing the gauge factor and minimizing the ink cost. The results indicate that a 1.78 mm line width and one layer are the optimal geometry parameters for the straight line traces, while for the horseshoe pattern, the optimal parameters are one layer, a line width of 1.78 mm, amplitude of 4 mm and one cycle. The optimal straight line was designed to sustain up to 10% strain while the horseshoe pattern was designed to sustain up to 15% strain.

Hydrotropic Extraction of Xanthones from Mangosteen Pericarp

2014 ◽  
Vol 984-985 ◽  
pp. 372-376 ◽  
Author(s):  
D. Gnana Prakash ◽  
Pradeep Panneerselvam ◽  
Sai Madhusudanan ◽  
V. Aditya
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Sodium Salicylate ◽  
Cost Effective ◽  
Separation Process ◽  
Extraction Technique ◽  
Solid Loading ◽  
Extraction Temperature ◽  
Optimal Conditions

A novel separation process for extraction of xanthones from mangosteen pericarp was investigated using aqueous hydrotrope solution. The hydrotropes sodium salicylate was used for the extraction. The effects of factors such as hydrotrope concentration, extraction temperature and solid loading were investigated by using Response Surface Methodology (RSM). The optimal conditions to obtain the highest yield of xanthones were 2M sodium salicylate, extraction temperature of 40°C and 3% solid loading. This hydrotropic extraction technique is simple and cost effective for the separation of bioactive compounds.Key words: Xanthones; Hydrotropes; Hydrotropic extraction

Response surface methodology (RSM) mediated interaction of media concentration and process parameters for the pigment production by Monascus purpureus MTCC 369 under solid state fermentation

2017 ◽  
Vol 46 (1) ◽  
pp. 14-20 ◽  
Author(s):  
Rachna Sehrawat ◽  
Paramjit S. Panesar ◽  
Tanya L. Swer ◽  
Anit Kumar
Keyword(s):  
Response Surface Methodology ◽  
Solid State ◽  
Response Surface ◽  
Solid State Fermentation ◽  
Cost Effective ◽  
Monascus Purpureus ◽  
Content Type ◽  
Pea Pod ◽  
Pharmaceutical Industries ◽  
Micro Organisms

Purpose This paper aims to extract colour from micro-organisms (as a source of natural pigments) using agro-industrial substrates to replace synthetic media by solid state fermentation. Nature is filled with colours. Due to health and environmental consciousness among people, use of synthetic colour has declined, and so the need to develop colour from cheap and easily available natural sources (plants, animals, micro-organisms and algae) using a cost-effective technique with higher yield and rapid growth. Monascus purpureus colour is a potent source of compounds (Dimerumic acid, Monacolin-k and -aminobutyric acid) having antimutagenic, antimicrobial and antiobesity, which helps in combating diseases. Design/methodology/approach Response surface methodology was used to optimise the biopigments extraction from Monascus purpureus using solid state fermentation. Findings The best optimised conditions for biopigments production using Monascus purpureus MTCC 369 were pH 5.4 at 32°C for 8 days 9 hours (8.9 days) from sweet potato peel and pea pod powder, 7.8 (w/w) and 3.9 per cent (w/w), respectively, which gave a final yield of 21 CVU/g. The model F-value of 69.18 and high value of adjusted determination coefficient 96.00 per cent implies high level of significance of the fitted model. Practical implications Extracted colour can be used in beverages, confectionery and pharmaceutical industries. Social implications Colour produced using Monascus purpureus MTCC 369 is a natural source. As consumers are reluctant to use synthetic colour because of the undesirable allergic reactions caused by them, so a biopigment produced is natural colouring compound with wide application in food sector. Originality/value Selected sources of carbon and nitrogen were not used earlier by any researcher to extract biopigment from Monascus purpureus MTCC 369.

An experimental strategy validated to design cost-effective culture media based on response surface methodology

2017 ◽  
Vol 47 (6) ◽  
pp. 578-588 ◽  
Author(s):  
J. L. Navarrete-Bolaños ◽  
M. G. Téllez-Martínez ◽  
R. Miranda-López ◽  
H. Jiménez-Islas
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Culture Media ◽  
Cost Effective ◽  
Experimental Strategy ◽  
Design Cost
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