Application of response surface methodology for optimization of growth and lipids in Scenedesmus abundans using batch culture system

RSC Advances ◽  
2014 ◽  
Vol 4 (42) ◽  
pp. 22129-22140 ◽  
Author(s):  
Chelladurai Chellamboli ◽  
Muthiah Perumalsamy
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Batch Culture ◽  
Culture System ◽  
Fossil Fuels ◽  
Biofuel Production ◽  
Alternative Source ◽  
System P

Owing to an increased demand for fuel and the depletion of fossil fuels, an alternative source such as algae is currently being exploited for biofuel production.

scholarly journals Response surface methodology for optimization studies of hydro-distillation of essential oil from pixie mandarin (Citrus reticulata Blanco) peels

2021 ◽  
Vol 23 (4) ◽  
pp. 26-34
Author(s):  
Tan Phat Dao ◽  
Ngo Thi Cam Quyen ◽  
Tran Thi Yen Nhi ◽  
Chi Cuong Nguyen ◽  
Trung Thanh Nguyen ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Essential Oil ◽  
Response Surface ◽  
Target Function ◽  
Extraction Process ◽  
Coefficient Of Determination ◽  
Gas Chromatography Mass Spectrometry ◽  
P Value ◽  
Alternative Source ◽  
Mathematical Techniques

Abstract Essential oil extraction technique from mandarin pixie peels by hydro-distillation is optimized by response surface methodology (RSM). Mathematical techniques were used in experimental design to evaluate the impacts of factors that affect the extraction process and improve the yield of the extraction process. A central mixed design based on influencing variables such as water ratio (3–5 mL/g), temperature (110–130 °C) and extraction time (90–150 min) was adopted with essential oil yield as the target function. Correlation analysis of the mathematical regression model showed that the quadratic polynomial model can be used to optimize hydro-distillation of pixie mandarin oil. The results showed that under the optimum extraction conditions, the highest quantity of essential oils was achieved (7.28 mL/100 g materials). In terms of statistical analysis, the significance levels (p-value <0.05) of the model showed that the experimental results had a good impact between factors. The coefficient of determination indicating the match between the experimental value and the predicted value of the model was high (R2>0.9). The chemical composition of the essential oil was analyzed by Gas Chromatography-Mass Spectrometry, revealing the dominance of limonene content (97.667%), which implies that the essential oil of pixie mandarin could be an alternative source of limonene.

scholarly journals Subcritical Water Extraction of Chlorella pyrenoidosa: Optimization through Response Surface Methodology

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Selvakumar Thiruvenkadam ◽  
Shamsul Izhar ◽  
Yoshida Hiroyuki ◽  
Razif Harun
Keyword(s):  
Response Surface Methodology ◽  
Reaction Time ◽  
Response Surface ◽  
Reaction Temperature ◽  
Subcritical Water ◽  
Chlorella Pyrenoidosa ◽  
Water Extraction ◽  
Biofuel Production ◽  
Oil Yield ◽  
Subcritical Water Extraction

Subcritical water extraction (SCW) was used to extract oil from Chlorella pyrenoidosa. The operational factors such as reaction temperature, reaction time, and biomass loading influence the oil yield during the extraction process. In this study, response surface methodology was employed to identify the desired extraction conditions for maximum oil yield. Experiments were carried out in batch reactors as per central composite design with three independent factors including reaction temperature (170, 220, 270, 320, and 370°C), reaction time (1, 5, 10, 15, and 20 min), and biomass loading (1, 3, 5, 10, and 15%). A maximum oil yield of 12.89 wt.% was obtained at 320°C and 15 min, with 3% biomass loading. Sequential model tests showed the good fit of experimental data to the second-order quadratic model. This study opens the great potential of SCW to extract algal oil for use in algal biofuel production.

scholarly journals Development of Sustainable Phycoremediation With the Help of Chlorella Pyrenoidosa for Textile Wastewater Analysis Using Response Surface Methodology

2020 ◽  
Author(s):  
Shubhangi Mishra ◽  
Pradeep Kumar Srivast ◽  
Virendra Singh ◽  
Monika Sharma
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Biomass Productivity ◽  
Textile Wastewater ◽  
Optimal Growth ◽  
Chlorella Pyrenoidosa ◽  
Biofuel Production ◽  
Aquatic Life ◽  
Chlorella Sp ◽  
Textile Wastewaters

Abstract The uncontrolled utilization for the textile products is increasing year by year resulting with the elevating wastewater generated from the textile industries, which makes it among the prevalent sources of critical environmental deteoration issue globally. Products obtained from the dyes used are the primary toxic product for aquatic life, they cause aesthetic pollution, eutrophication, perturbation and increase in BOD and COD in aquatic life. Three types of textile wastewaters (Acid Yellow dye, Acid orange dye and Basic pink dye) has been used for wastewater treatment and microalgal (Chlorella pyrenoidosa) biomass production. Nitrogen content in textile wastewaters is very less, hence urea is used as nitrogen source in wastewater. Optimal growth condition (Urea-0.4g/L, wastewater- 40%(v/v)) is developed through Response surface methodology (RSM). The biomass productivity for chlorella sp. is 1.2-1.5 g/L/day in textile wastewaters. The reduction efficiency of COD, Nitrate-N Ammonia-N, Phosphate-P, and Dye(color) removal for Chlorella is 90-95%, 75-85%, 90-98%, 65-74% and 40-65%.After harvesting the Biomass by flocculation method it can be used for biofuel production by in-situ transesterification.

scholarly journals Exploring the Exhaust Emission and Efficiency of Algal Biodiesel Powered Compression Ignition Engine: Application of Box–Behnken and Desirability Based Multi-Objective Response Surface Methodology

Energies ◽  
2021 ◽  
Vol 14 (18) ◽  
pp. 5968
Author(s):  
Prabhakar Sharma ◽  
Ajay Chhillar ◽  
Zafar Said ◽  
Saim Memon
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Fossil Fuels ◽  
Objective Response ◽  
Interaction Model ◽  
Operating Conditions ◽  
Exhaust Emission ◽  
Operating Parameters ◽  
Compression Ignition Engine ◽  
Multi Objective

Sustainable Development Goals were established by the United Nations General Assembly to ensure that everyone has access to clean, affordable, and sustainable energy. Third-generation biodiesel derived from algae sources can be a feasible option in tackling climate change caused by fossil fuels as it has no impact on the human food supply chain. In this paper, the combustion and emission characteristics of Azolla Pinnata oil biodiesel-diesel blends are investigated. The multi-objective response surface methodology (MORSM) with Box–Behnken design is employed to decrease the number of trials to conserve finite resources in terms of human labor, time, and cost. MORSM was used in this study to investigate the interaction, model prediction, and optimization of the operating parameters of algae biodiesel-powered diesel engines to obtain the best performance with the least emission. For engine output prediction, a prognostic model is developed. Engine operating parameters are optimized using the desirability technique, with the best efficiency and lowest emission as the criteria. The results show Theil’s uncertainty for the model’s predictive capability (Theil’s U2) to be between 0.0449 and 0.1804. The Nash–Sutcliffe efficiency is validated to be excellent between 0.965 and 0.9988, whilst the mean absolute percentage deviation is less than 4.4%. The optimized engine operating conditions achieved are 81.2% of engine load, 17.5 of compression ratio, and 10% of biodiesel blending ratio. The proposed MORSM-based technique’s dependability and robustness validate the experimental methods.

scholarly journals Development of Sustainable Phycoremediation with the help of Chlorella Pyrenoidosa for Textile Wastewater Analysis using Response Surface Methodology

2020 ◽  
Author(s):  
Shubhangi Mishra ◽  
Pradeep Srivast ◽  
Virendra Singh ◽  
Monika Sharma
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Biomass Productivity ◽  
Textile Wastewater ◽  
Optimal Growth ◽  
Chlorella Pyrenoidosa ◽  
Biofuel Production ◽  
Aquatic Life ◽  
Chlorella Sp ◽  
Textile Wastewaters

Abstract The uncontrolled utilization for the textile products is increasing year by year resulting with the elevating wastewater generated from the textile industries, which makes it among the prevalent sources of critical environmental deteoration issue globally. Products obtained from the dyes used are the primary toxic product for aquatic life, they cause aesthetic pollution, eutrophication, perturbation and increase in BOD and COD in aquatic life. Three types of textile wastewaters (Acid Yellow dye, Acid orange dye and Basic pink dye) has been used for wastewater treatment and microalgal (Chlorella pyrenoidosa) biomass production. Nitrogen content in textile wastewaters is very less, hence urea is used as nitrogen source in wastewater. Optimal growth condition (Urea-0.4g/L, wastewater- 40%(v/v)) is developed through Response surface methodology (RSM). The biomass productivity for chlorella sp. is 1.2-1.5 g/L/day in textile wastewaters. The reduction efficiency of COD, Nitrate-N Ammonia-N, Phosphate-P, and Dye(color) removal for Chlorella is 90-95%, 75-85%, 90-98%, 65-74% and 40-65%.After harvesting the Biomass by flocculation method it can be further used for biofuel production by in-situ transesterification.

scholarly journals Optimization of pectin extraction from the native fruit of Garcinia binucao using response surface methodology

2019 ◽  
Vol 268 ◽  
pp. 01006
Author(s):  
Jed Karl Victor Robrigado ◽  
Jasen Emil De Leon ◽  
Aldous Tyrone Orillan ◽  
John Raymond Barajas ◽  
Pee Jay Gealone
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Disease Outbreaks ◽  
Extraction Process ◽  
World Market ◽  
The Philippines ◽  
Extraction Time ◽  
Alternative Source ◽  
Promising Alternative ◽  
Citrus Greening Disease

Citrus supply for pectin primers in the world market is continually under threat due to the widespread of uncontrolled citrus greening disease outbreaks. As a consequence, a market squeeze in pectin supply was experienced globally and further resulted to a significant surge in prices of pectin derived products. In an attempt to provide an alternative source of pectin primers to the pectin-making industry, we investigate a novel and promising pectin primer native to the Philippines. In this work, we explored extraction of pectin from fruits of the Garcinia binucao tree (GBT). We implement a response surface methodology (RSM) to optimize the extraction of pectin from GBT. Box-behken experimental design was used to obtain the optimal conditions in the extraction process. Temperature (°C), extraction time (min), and pH were found to have significant effects on the pectin yield. Actual values of these independent variables were chosen on the basis of preliminary experimental results. Optimum conditions using ridge analysis were found to be: temperature 72.4°C, extraction time 117.5 min, and pH at 1.68. In conclusion, a high pectin yield obtained from GBT fruits strongly suggests that these fruits are promising alternative primers in pectin production.

scholarly journals Endophytic bacteria of Catharanthus roseus as an alternative source of Vindoline and application of response surface methodology to enhance its production

2019 ◽  
Vol 71 (1) ◽  
pp. 27-38 ◽  
Author(s):  
Nawed Anjum ◽  
Ramesh Chandra
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Catharanthus Roseus ◽  
Endophytic Bacteria ◽  
Cost Effective ◽  
Ionization Mass ◽  
Nutritional Requirement ◽  
Alternative Source ◽  
Anticancer Compounds ◽  
Cost Efficient

The requirement for novel, cost-effective pharmaceutical compounds is growing. We screened endophytic bacteria of Catharanthus roseus for the production of vinca alkaloids. Vindoline-producing endophytic bacteria was identified as Microbacterium sp. Vindoline was purified and characterized using column chromatography, TLC, HPLC and electrospray ionization mass spectrometry. The subsequent aim of this study was to design a cost-efficient media that can maximize vindoline production from Microbacterium sp. Initial optimization of the nutritional requirement and of the process parameters were carried out by monothetic analysis. Key factors obtained were optimized by the central composite design of response surface methodology. This model was also validated by repeating the experiment under the optimized conditions, which resulted in maximum production of 82 ?g/L (predicted response was 76.21 ?g/L). Reliability of the experiments was confirmed by ANOVA analysis, regression coefficient, prediction vs actual chart and a 3D response plot, which determined the fit of the model. This study should serve as a reference point for the use of statistical techniques in bacterial systems for production of bioactive, anticancer compounds by plant endophytes.

scholarly journals Exploring the potentials of microalgae as an alternative source of renewable energy

2021 ◽  
Vol 38 (1) ◽  
pp. 24-39
Author(s):  
P.A. Adedibu ◽  
D.A. Animasaun ◽  
G.G. Joseph
Keyword(s):  
Renewable Energy ◽  
Fossil Fuels ◽  
Economic Value ◽  
Biofuel Production ◽  
Food Sources ◽  
Alternative Source ◽  
Plant Materials ◽  
Conventional Agriculture ◽  
High Productivity ◽  
Alternative Means

The world’s energy needs highly depend on fossil fuels, which were formed over several million centuries. The price of petroleum increases daily and unfortunately, its exploitation is currently at an alarming rate for such essential non-renewable energy. Also, the recent clamour for safe and cheap alternative means of energy generation to mitigate global warming and its detrimental effects is drawing attention towards biofuel production to supplement and possibly, substitute fossil fuels. To this effect, many plant materials have been tested and employed in the past decades for biofuel production. However, a good number of plants used in biofuel production as feedstock are crop plants, which have more economic value as food. Therefore, it is imperative to explore the possibility of biofuel production from non-food sources, hence, we examine the potential of microalgae as an alternative source of renewable energy. Microalgae are of great interest in biofuel production for its high productivity, cosmopolitan nature, easy culturing on waters and land, and noncompeting with conventional agriculture for resources. In view of these, this article focuses on the potentials of microalgae in biofuel production and mitigation of environmental pollution by its considerably low greenhouse gas emissions.

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