scholarly journals The effect of knockdown of insulin receptor substrates 1 and 2 on glucose and lipid metabolism in human hepatoblastoma cells

2018 ◽  
Vol 70 (4) ◽  
pp. 757-764
Author(s):  
Tianfei Yu ◽  
Ming Li ◽  
Huiying Dong ◽  
Pengyu Xie ◽  
Wanzhu Sun ◽  
...  

The European corn borer (Ostrinia nubilalis) is one of the most important insect pests of maize, and has a significant impact on the production of this crop. In this work, we examined the effects of different insecticides on the antioxidative defense system of O. nubilalis larvae. The experimental setup consisted of a completely randomized block design with 4 replicates. Four experimental groups were formed as follows: control (C), indoxacarb (250 mL ha-1), chlorantraniliprole (100 mL ha-1) and the chlorantraniliprole+lambda cyhalothrin (200 mL ha-1) group. Larvae from maize stems were collected 20 days after insecticide application and the whole larvae were homogenized. The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione S-transferase (GST), and the total amount of free SH groups were assessed. Comparison of the experimental groups showed that indoxacarb significantly affected the activities of GST, GPx and the total amount of free SH groups, while chlorantraniliprole significantly affected the activities of SOD, CAT, GST and the total amount of free SH groups, while chlorantraniliprole+lambda cyhalothrin significantly affected the activities of CAT, GST and the total amount of free SH groups. The results show that exposure to insecticides considerably affects the antioxidative defense components of the European corn borer larvae, especially chlorantraniliprole (T2).

2018 ◽  
Vol 70 (4) ◽  
pp. 765-773 ◽  
Author(s):  
Filip Franeta ◽  
Dejan Mircic ◽  
Dajana Todorovic ◽  
Zeljko Milovac ◽  
Nihat Granica ◽  
...  

The European corn borer (Ostrinia nubilalis) is one of the most important insect pests of maize, and has a significant impact on the production of this crop. In this work, we examined the effects of different insecticides on the antioxidative defense system of O. nubilalis larvae. The experimental setup consisted of a completely randomized block design with 4 replicates. Four experimental groups were formed as follows: control (C), indoxacarb (250 mL ha-1), chlorantraniliprole (100 mL ha-1) and the chlorantraniliprole+lambda cyhalothrin (200 mL ha-1) group. Larvae from maize stems were collected 20 days after insecticide application and the whole larvae were homogenized. The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione S-transferase (GST), and the total amount of free SH groups were assessed. Comparison of the experimental groups showed that indoxacarb significantly affected the activities of GST, GPx and the total amount of free SH groups, while chlorantraniliprole significantly affected the activities of SOD, CAT, GST and the total amount of free SH groups, while chlorantraniliprole+lambda cyhalothrin significantly affected the activities of CAT, GST and the total amount of free SH groups. The results show that exposure to insecticides considerably affects the antioxidative defense components of the European corn borer larvae, especially chlorantraniliprole (T2).


2009 ◽  
Vol 24 (1) ◽  
pp. 19-24 ◽  
Author(s):  
Kathleen Russell ◽  
Ric Bessin

AbstractA two-year field study was conducted to evaluate the effectiveness of Trichogramma ostriniae Pang et Chen (T.o.) releases in combination with habitat modification, to enhance biological control of European corn borer (ECB), Ostrinia nubilalis Hübner, the key pest of bell peppers in Kentucky. A randomized block design with a split-plot treatment arrangement was used in both years. Buckwheat, Fagopyrum esculetum Moench, was inter-planted as a nectar source for the wasps and as an attractant for ECB natural enemies. Sentinel ECB egg masses were placed in each subplot to monitor establishment and dispersal of T. ostriniae within and between plots. Fruit were harvested at maturity, graded according to USDA standards, inspected for insect damage, and dissected to confirm ECB presence when evidence of entry was suspected, to determine percentage of infested fruits. Percent ECB infested fruit was reduced in plots with wasp releases and in plots with buckwheat inter-planted. The interaction of habitat modification and wasp releases significantly reduced pepper damage as a result of the synergy between tactics. Percentage of T. ostriniae parasitized sentinel eggs was significantly higher in wasp release plots compared to non-release plots. T. ostriniae releases used in conjunction with habitat modification for ECB control in bell peppers appears to be a promising alternative to traditional insecticide-based strategies for small-acreage and organic farms. This study displays an example system utilizing conservation biological control at a landscape level for pest suppression.


2011 ◽  
Vol 51 (1) ◽  
pp. 38-43 ◽  
Author(s):  
Jagdev Kular ◽  
Sarwan Kumar

Quantification of Avoidable Yield Losses in OilseedBrassicaCaused by Insect PestsA six year field study was conducted from 2001-2002 to 2006-2007 at Punjab Agricultural University, Ludhiana, India to study the losses in seed yield of differentBrassicaspecies (B. juncea, B. napus, B. carinata, B. rapaandEruca sativa) by the infestation of insect pests. The experiment was conducted in two different sets viz. protected/sprayed and unprotected, in a randomized block design, with three replications. Data on the infestation of insect pests, and seed yield were recorded at weekly intervals and at harvest, respectively. The loss in seed yield, due to mustard aphid and cabbage caterpillar, varied from 6.5 to 26.4 per cent.E. sativasuffered the least loss in seed yield and harboured the minimum population of mustard aphid (2.1 aphids/plant) and cabbage caterpillar (2.4 larvae/plant). On the other hand,B. carinatawas highly susceptible to the cabbage caterpillar (26.2 larvae/plant) and suffered the maximum yield loss (26.4%).


2021 ◽  
Author(s):  
Huyen Bui ◽  
Robert Greenhalgh ◽  
Gunbharpur S. Gill ◽  
Meiyuan Ji ◽  
Andre H. Kurlovs ◽  
...  

AbstractMaize (Zea mays subsp. mays) yield loss from arthropod herbivory is substantial. While the basis of resistance to major insect herbivores has been comparatively well-studied in maize, less is known about resistance to spider mite herbivores, which are distantly related to insects and feed by a different mechanism. Two spider mites, the generalist Tetranychus urticae, and the grass-specialist Oligonychus pratensis, are notable pests of maize, especially during drought conditions. We assessed the resistance to both mite species of 38 highly diverse maize lines, including several previously reported to be resistant to one or the other mite species. We found that line B96, as well as its derivatives B49 and B75, were highly resistant to T. urticae. In contrast, neither these three lines, nor any others included in our study, were notably resistant to O. pratensis. Quantitative trait locus (QTL) mapping with F2 populations from crosses of B49, B75, and B96 to susceptible B73 identified a large-effect QTL on chromosome 6 as underlying T. urticae resistance in each line, with an additional QTL on chromosome 1 in B96. Genome sequencing and haplotype analyses identified B96 as the apparent sole source of resistance haplotypes. Our study identifies loci for use in maize breeding programs for T. urticae resistance, as well as to assess if the molecular-genetic basis of spider mite resistance is shared with insect pests of maize, as B96 is also among the most resistant known maize lines to several insects, including the European corn borer, Ostrinia nubilalis.Key message Maize(Zea mays subsp. mays) inbred lines B49, B75, and B96 harbor large-effect loci for resistance to the generalist spider mite Tetranychus urticae, but not the specialist Oligonychus pratensis.


EUGENIA ◽  
2015 ◽  
Vol 21 (1) ◽  
Author(s):  
J. Rimbing ◽  
J. Pelealu ◽  
D. Sualang

ABSTRACT   The research was arranged in a randomized block design (RBD), with 10 treatments and 3 replications. To get the active insects pests, net sweepings were done for the flying insects, while other pests were observed directly on the plants. Collecting data of plant damages were made by systematic diagonal lines, except for viruses, census was conducted on each experimental plot.  The results of this experiment documented 12 species of insect pests attacking soybean plants. Soybean pest populations were relatively low, except for Aphis glycine that was quite high. In all treatments, plant damage caused by sucking pest Piezodorus sp and Riptortus linearis were relatively low of 1.53 % to 1.94 %; it indicated an insignificant effect to decline the production. Crop damages by virus showed a significant effect. The lowest virus attacks found on compost of 5 tons per ha with 43 plants infected per treatment, the highest virus attack showed on combination of 5 tons compost per ha + inorganic fertilizer + mulch, in which there were 274 infected plants per treatment. Apparently virus attacks gave impact on soybean production. The highest production were documented on treatment of 5 tons compost per ha with production of 0.55 tons per ha, while the lowest production was found on combination of 5 tons compost per ha + inorganic fertilizer + mulch, in which the production was 0.22 tons per ha. Keywords:  fertilizer, mulch, insect pest, production


1994 ◽  
Vol 19 (1) ◽  
pp. 131-131
Author(s):  
K. A. Sorensen ◽  
C. Wade Holloway

Abstract ‘Atlantic’ potatoes were planted in early Mar for Tests 1 and 2 near Elizabeth City, N.C. on 42 inch centers. Plots in Test 1 were 14 ft long and in Test 2 20 ft long, with single row spacing replicated 4 times in a randomized complete block design. Alleys of 5 ft were used between replicates within rows. Treatments in Test 1 and Test 2 were applied with a CO2 pressurized backpack sprayer with a single hollow cone nozzle (18X) operated at 60 psi to deliver 54.5 gal/acre. In Test 1 all treatments were applied on 5, 11 and 18 May. All treatments were applied to Test 2 on 5 May, with the exception of the 1/6 lb rate of Trigard 75WP in combination with Trigard 75WP at 1/3 lb rate which received the combination low rate (1/6 lb) 6 days later. On 11 May applications were applied to all treatments except for Trigard 75WP at the 1/3 lb, 2/3 lb (egg only) and Trigard 75WP 1/3 lb (egg only) plus Trigard 75WP at the 1/6 lb rate. At this time the low rate of the combination Trigard 75WP 1/6 lb rate was applied. Imidan 50WP at 2 lbs and Imidan 50WP at 2 lbs plus Butacide 8EC at 0.5 lbs was applied on 5, 11 and 18 May. Test 1 and Test 2 counts of CPB on 3/hill plots were made 11 and 18 May. Small larvae were assumed to be first and second instar, and large larvae, third and fourth instar. Visual CPB damage ratings were made 18 May and 15 Jun. Visual ECB ratings were made and potatoes harvested and yields recorded 15 Jun.


2020 ◽  
Vol 13 (4) ◽  
pp. 125
Author(s):  
IG.A.A. INDRAYANI ◽  
SIWI SUMARTINI

<p>ABSTRAK<br />Hingga kini teknik perakitan varietas kapas tahan hama masih<br />dilakukan secara konvensional berdasarkan beberapa karakter morfologi<br />tanaman, seperti: bulu daun, daun okra, braktea berpilin, nektar, dan<br />gosipol tinggi. Karakter-karakter ini diketahui erat hubungannya dengan<br />ketahanan terhadap hama, khususnya H. armigera. Berkaitan dengan<br />serangan H. armigera pada buah, diduga ada bagian-bagian buah kapas<br />yang berkontribusi secara langsung pada serangan hama ini, misalnya<br />braktea buah. Namun demikian, besarnya pengaruh braktea terhadap<br />kerusakan buah kapas perlu dipelajari dalam upaya meminimalkan<br />kerusakan. Penelitian ini bertujuan untuk mengetahui pengaruh ukuran<br />braktea terhadap tingkat kerusakan buah oleh H. armigera pada beberapa<br />aksesi kapas. Penelitian dilaksanakan di Kebun Percobaan Balai Penelitian<br />Tanaman Tembakau dan Serat, di Asembagus, Situbondo, Jawa Timur<br />mulai bulan Januari hingga Desember 2006. Sebanyak 18 aksesi dari 50<br />aksesi kapas dengan berbagai variasi ukuran braktea digunakan sebagai<br />perlakuan. Setiap perlakuan (aksesi) disusun dalam rancangan acak<br />kelompok (RAK), dengan tiga kali ulangan. Lima tanaman kapas dari<br />masing-masing aksesi ditentukan secara acak, dan sebanyak 5 buah kapas<br />muda (diameter ± 4 cm) dipetik dari masing-masing tanaman sampel,<br />kemudian dibawa ke laboratorium untuk diukur luas braktea dan buahnya.<br />Selain itu dilakukan pula pengamatan kerusakan buah dan hasil kapas<br />berbiji di lapang. Hasil penelitian menunjukkan bahwa ukuran braktea<br />berkorelasi positif dengan tingkat kerusakan buah (R 2 = 0,9014), sehingga<br />braktea berukuran besar dan lebar serta menutupi buah secara total<br />berpotensi mengalami kerusakan akibat serangan H. armigera lebih tinggi<br />dibanding braktea berukuran kecil dan sempit. Ukuran panjang dan lebar<br />braktea pada 18 aksesi kapas bervariasi antar aksesi dan masing-masing<br />berkorelasi positif dengan luas (R 2 = 0,876; R 2 = 0,894). Hasil penelitian<br />ini dapat dimanfaatkan dalam merakit varietas tahan hama, dan<br />kombinasinya dengan karakter-karakter morfologi kapas yang sudah ada<br />untuk menghasilkan varietas kapas baru dengan tingkat ketahanan yang<br />lebih tinggi terhadap hama penggerek buah H. armigera.<br />Katakunci : Braktea, Helicoverpa armigera, aksesi kapas, karakter<br />morfologi.</p><p><br />ABSTRACT<br />Effects of bract size of several cotton accessions to<br />American bollworm injury level<br />Conventional  method  by  crossing  technique  based  on<br />morphological characters of plant is now still used in providing resistant<br />varieties of cotton against insect bollworms. A number of genetic<br />characters are now available and have been studying for their assosiation<br />with insect pests resistance such as hairiness, okra leaf, frego bract,<br />nectariless, and high gossypol. Regarding to boll damage by H. armigera,<br />it can be mentioned that there are many other morphological characters of<br />cotton attributable to bollworm damage, such as floral bract. As a part of<br />boll, it is estimated that bracts assosiated with bollworm attacked due to<br />their larger size compared with boll size. Objective of the study was to find<br />out the effect of bract size in relation to bollworm damage on cotton<br />accessions. The study was conducted at Experimental Station of<br />Indonesian Tobacco and Fiber Crops Research Institute in Asembagus,<br />Situbondo, East Java from January to December 2006. Eighteen of fifty<br />cotton accessions were used as treatment and they were arranged in<br />Randomized Block Design (RBD) with three replications. Five randomly<br />cotton plants from each accession and five young bolls were sampled<br />from the selected plant with about 4 cm of diameter were brought in the<br />laboratory to collect information on bract and boll sizes. Bollworm<br />damage was determined by counting the damaged bolls in the field as well<br />as the seed cotton yield. Result showed that bract size was positively<br />correlated with boll damage (R 2 = 0.9014). Higher damaged bolls occured<br />on bolls which is covered completely by bracts. There is variation between<br />length and wide size of bracts among cotton accessions and both showed<br />positive correlation to bract area (R 2 = 0.876; R 2 = 0.894). Based on this<br />study, higher resistance of cotton variety against H. armigera will<br />possiblly be provided through combination between bract size and any<br />other morphological characters of cotton.<br />Key words : Floral bract, Helicoverpa armigera, cotton accession,<br />morphological character</p>


Author(s):  
K. L. Naga ◽  
A. R. Naqvi ◽  
B. L. Naga ◽  
H. L. Deshwal ◽  
L. Jhumar

Aim: Some genotypes of mothbean were screened for their comparative preference against jassids, whiteflies and thrips. Materials and Methods: The experiment was laid out during Kharif season in Randomized Block Design with three Replications. Ten genotypes (viz., RMO-225, RMO-40, RMO-423, RMO-435, RMO-257, RMO-25, RMO-141, RMO-20-36, RMO-04-01-28 and RMO-28-80) were screened against major sucking insect pests which were replicated thrice. Observations: The observations were recorded after two weeks of sowing at weekly intervals after two weeks of sowing. The pest populations were recorded on five randomly selected and tagged plants per plot in early hours when insect have minimum activity. Results: The data revealed that none of the genotypes ware found free from sucking insect pest attack. On the basis of peak population, the genotypes RMO-25 and RMO-141 were categorized as least preferred to jassids, whiteflies and thrips, whereas, RMO-435, RMO-225 and RMO-04-01-28 as highly preferred to jassids and whiteflies and RMO-435, RMO-225 and RMO-257 as highly resistant to thrips. Conclusion: It is well known that certain genotypes of crops are less attacked by a specific insect-pest than others because of natural resistance. In the integrated pest management programme, growing of varieties (RMO-25 and RMO-141) with less preference to sucking insect-pests is one of the most important tools without additional cost.


2010 ◽  
Vol 58 (1) ◽  
pp. 73-79
Author(s):  
F. Pál-Fám ◽  
Z. Varga ◽  
S. Keszthelyi

A better understanding of the relationships between insects and microfungi could help to identify the unknown factors reducing yields in maize. As the first step in current research, the aim was to isolate the microfungal species that can be found in the larval cavity of the European corn borer ( Ostrinia nubilalis Hbn. Lepidoptera. Pyraustidae ) (ECB), one of the most important insect pests of maize. In this way, the scale of potential phytopathogens spread by intermediate hosts could be reduced.Fifty stalk sections damaged by ECB larvae were collected in autumn and fifty in spring on a 20-hectare plot in Ráksi (Somogy county). These were placed in wet chambers and incubated at room temperature under natural light. Identification was done from a pure culture inoculated into potato dextrose agar. Twenty-one species from 14 fungus genera were identified, the majority of which were mitosporic fungi. Species belonging to the Fusarium, Acremoniella and Cladosporium genera were predominant. Most of the species were saprotrophic, though some phytopathogenic species ( Gibberella, Colletotrichum, Nigrospora and Fusarium ) were also identified. The number of genera and the incidence of fungi were much higher in spring samples than in autumn ones, except for Fusarium , where incidence was lower in spring. It was found that failing to harvest the maize significantly enhanced the spread of several fungus species, especially phytopathogenic species, the following year, thereby serving as a source of infection.


Agrologia ◽  
2018 ◽  
Vol 1 (2) ◽  
Author(s):  
Johan A Patty

Plant pests Spodoptera litura is one of the major insect pests that often lowers the cabbage yield. Various control techniques of this pest have been developed, such as the use of botanical pesticides. This study aimed to determine the role of aromatic plants in reducing S. litura population growth and plant damage intensity on cabbage. Experiments used intercropping combinations of cabbage-local basil (‘kemangi’), cabbage-tomato, cabbage-scallion and without aromatic plants as a control. The experimental was arranged in a Randomized Block Design. Parameters measured were larval population, damage intensity and fresh weight of cabbage crops. The results showed that all three cabbage intercropping with the aromatic plants were able to suppress population of S. litura and the resulted damage intensity of cabbage. The treatment of cabbage intercropping with local basil could reduce larval populations of S. litura (to 0.84 larvae) or only 8 larvae per 10 plants and with the lowest crop damage intensity (3.32%) and highest crop weight (486.6 g) per plant.


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