scholarly journals Effects of increased proliferation of human adipose tissue-derived mesenchymal stem cells by sphingosylphosphorylcholine on the survival of cryopreserved fat grafts

2018 ◽  
Vol 70 (3) ◽  
pp. 543-549
Author(s):  
Yong Bae ◽  
Ji Song ◽  
Kyeong Nam ◽  
Joo Kim ◽  
Su Nam
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Nude Mice ◽  
Soft Tissue Augmentation ◽  
Fat Graft ◽  
Saline Control ◽  
Control Group ◽  
Fat Tissue ◽  
Lipid Molecule ◽  
Fat Grafts

The use of cryopreserved adipose tissue for soft-tissue augmentation is common, but the unpredictability of fat graft viability remains a limitation. Human adipose-derived stem cells (hADSC) have been introduced to enhance viability and improve the survival of transplanted fat tissue. Sphingosylphosphorylcholine (SPC) is a bioactive lipid molecule involved in various cellular responses. SPC stimulates the proliferation of various cell types such as hADSC. We demonstrated the effects of hADSC and SPC on the survival of cryopreserved fat grafts in nude mice. The cryopreserved fat grafts were treated with hADSC or hADSC+SPC, and a normal saline (control) mixture in BALB/c male nude mice. We examined the weight and volume of the mice in each group (n=11) at 8 weeks after transplantation to evaluate the survival of the fat tissue. The hADSC group showed increased weight and volume compared with the control group. The hADSC+SPC group showed a higher survival rate in terms of weight and volume than the control or hADSC group. In addition, the hADSC+SPC treatment significantly increased the expression of angiogenic factors. These results suggest the potential clinical utility of hADSC+SPC.

scholarly journals Improvement of the Survival of Human Autologous Fat Transplantation by Adipose-Derived Stem-Cells-Assisted Lipotransfer Combined with bFGF

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Aimei Jiang ◽  
Ming Li ◽  
Wenjing Duan ◽  
Yilong Dong ◽  
Yanmei Wang
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Histological Analysis ◽  
Angiogenic Factors ◽  
Fat Graft ◽  
Adipose Derived Stem Cells ◽  
Fat Transplantation ◽  
Autologous Fat ◽  
Fat Grafts ◽  
Autologous Fat Transplantation

Adipose-derived stem cells (ASCs) transplanted along with autologous adipose tissue may improve fat graft survival; however, the efficacy of ASCs has been diluted by low vascularization. This study was designed to test the hypothesis that basic fibroblast growth factor (bFGF) may improve the effects of ASCs because it owns the property to boost angiogenesis. In the present study, human fat tissues were mixed with ASCs, ASCs plus 100 U bFGF, or medium as the control and then injected subcutaneously into immunologically compromised nude mice for 12 weeks. Our findings demonstrated that mixture with the ASCs significantly increased the weight and volume of the fat grafts compared to control grafts, and histological analysis revealed that both ASCs and ASCs plus bFGF grafts consisted predominantly of adipose tissue and had significantly less fibrosis but greater microvascular density compared with control and also grafts mixed with ASCs had a high expression of angiogenic factors. More importantly, the bFGF treated fat grafts shown elevate in survival, vascularization, and angiogenic factors expression when compared with the grafts that received ASCs alone. These results indicated that bFGF together with ASCs can enhance the efficacy of autologous fat transplantation and increase blood vessel generation involved in the benefits from bFGF.

scholarly journals Comparative study on the effect of adipose-derived stem cells versus alpha-lipoic acid in autologus fat graft survival in adult female albino rat: histological and immune histochemical study

QJM ◽  
2020 ◽  
Vol 113 (Supplement_1) ◽  
Author(s):  
S H Ibrahim ◽  
S M M Omar ◽  
G A Ibrahim ◽  
A E Omar
Keyword(s):  
Stem Cells ◽  
Survival Rate ◽  
Lipoic Acid ◽  
Fat Graft ◽  
Control Group ◽  
Adipose Derived Stem Cells ◽  
Alpha Lipoic Acid ◽  
Inflammatory Infiltration ◽  
Autologous Fat ◽  
Fat Grafts

Abstract Background Autologous fat transfer is a popular option for soft tissue augmentation, but has a low survival rate. So, enriching the transplanted fat with stem cells (cell assisted lipotransfer) or oral administration of alpha-lipoic acid (ALA) before and after graft injection, were thought to increase the survival rate of the transplanted fat. Aim of Study Is to compare between cell-assisted lipotransfer (CAL) and ALA therapy in improvement of fat graft survival. Materials and Methods Thirty adult female albino rats were used and divided into three groups. Group I: the control group. Group II: CAL group received autologous fat mixed with adipose derived stem cells (ASCs). Group III: ALA group in which animals received oral ALA four days before fat transplantation and continued throughout experiment. Fat grafts were collected from the sites of injection after twenty-eight days. Histological and immune histochemical studies were performed. Statistical analysis was also done. Results Histological evaluation of the fat grafts from animals of control group revealed atrophy and death of adipocytes with formation of fat cysts. Severe inflammatory infiltration with giant cells formation and fibrosis were detected. Significant decrease of VEGF reaction was seen. In CAL group significant increase in number of intact adipocytes together with decrease in inflammation and fibrosis occurred. Moreover, significant increase in VEGF reaction was observed. In ALA group, significant decrease in number of intact adipocytes and VEGF reaction was detected compared with CAL group. Also, significant increase in inflammatory infiltration and fibrosis were detected compared to CAL group. Conclusions Both ASCs and ALA showed improvement in all histological parameters compared with control group. However, enrichment of fat with ASCs showed the best results.

Abstract 1281: Human Adipose Tissue-derived Stem Cells Potently Preserve Cardiac Function Resulting From Permanently Induced Myocardial Ischemia

Circulation ◽  
2007 ◽  
Vol 116 (suppl_16) ◽  
Author(s):  
Liying Cai ◽  
Brian H Johnstone ◽  
Todd G Cook ◽  
Keith L March
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Cardiac Function ◽  
Heart Function ◽  
Human Adipose Tissue ◽  
Functional Improvement ◽  
Saline Control ◽  
Control Group

Background The use of stem cells has gained much interest as a potential therapeutic approach for repair of damage caused by a variety of cardiac insults. We and others have demonstrated the ability of pluripotent adipose-derived stem cells (ASCs), to promote repair in ischemic skeletal tissues. Here we demonstrate that ASCs also stimulate a robust functional improvement following acute myocardial infarction (MI) in rats. Methods and Results ASCs were harvested from human subcutaneous adipose tissue. ASCs were characterized in vitro before in vivo testing. Growth and metabolic activity of human microvascular endothelial cells (HMVEC) cultured in growth-factor deficient minimal medium (MM) increased 1.7-fold when supplemented with a 1:1 mixture of ASC CM ( p <0.01). Sprout formation by HMVECs and migration of endothelial progenitor cell (EPC) was enhanced by 2.1 and 2.0-fold, repectively, when ASC CM was added to MM ( p <0.01). Following demonstration of potency in vitro, the ASCs were evaluated for the ability to protect and rescue ischemic myocardium in an athymic nude rat following permanent ligation of the proximal LAD region. Immediately after ligation 10 6 ASCs in 100 μl saline or carrier alone was injected into 2 sites of the peri-infarct region, then at 4 and 28 d heart function was evaluated echocardiography using a Visualsonics Vivo770. ASC-treated rats consistently exhibited better cardiac function at 1 month compared to the saline control group. LV ejection fraction of the ASC group was 56 ± 7% (mean ± SEM) vs 37 ± 3% for the control (p<0.04). Fractional shortening was 32 ± 5% (ASC) vs 19 ± 2% (p<0.04). LV volumes both at end-diastolic and end-systolic stages were lower in ASC group (311 ± 17 μl and 139 ± 21 μl, respectively) than saline group (391 ± 30 μl and 249 ± 27 μl) (p<0.03). Anterior wall thinning was attenuated in ASC group (1.6 ± .08 mm vs 1.2 ± .2, at end-diastole, p<0.03). Post-mortem histological analysis demonstrated that ASC treated hearts had lower fibrosis (26 ± 6% vs 34 ± 6%; p<0.05). Conclusion We have demonstrated that ASCs have great potential as a cell therapy to preserve heart function following ischemic insult. Given the abundant source of ASCs, therapies with these cells have a higher potential for widespread adoption compared to more rare cell types.

scholarly journals Human Umbilical Cord Mesenchymal Stem Cell Promotes Angiogenesis via Integrin β1/ERK1/2/HIF-1α/VEGF-A Signaling Pathway for off-the-shelf Breast Tissue Engineering

2021 ◽  
Author(s):  
Mian Wu ◽  
Lifeng Chen ◽  
Yuhan Qi ◽  
Hai Ci ◽  
Shan Mou ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Umbilical Cord ◽  
Breast Tissue ◽  
Control Group ◽  
Human Umbilical Cord ◽  
Fat Tissue ◽  
Integrin Β1

Abstract Background: Mesenchymal stem cells (MSC) based tissue engineered breast represent the visible future for breast reconstruction after mastectomy. However, autologous MSCs might not be appropriate for the large graft construction due to cell senescence during excessive cell expansion, thus hindering its further off-the-shelf application. As human umbilical cord stem cells (hUCMSCs) have been proved to be safe with low immune response and easily stored, they are ideal for off-the-shelf tissue engineering application. Here, we aim to explore the possibility of umbilical cord mesenchymal stem cells as tissue-engineered breast seed cells. Methods: The allogenic hUCMSCs were injected into transplanted fat tissue with or without breast scaffolds as an alternative for breast tissue engineering in vivo, and its potential mechanism of angiogenesis in vitro was explored.Results: The hUCMSCs promoted proliferation, migration, and angiogenesis of human umbilical vein endothelial cells (HUVECs) through paracrine process by activating the integrin β1/ERK1/2/HIF-1α/VEGF-A signaling pathway. Histological examination of grafted fat revealed that the group which received hUCMSCs transplantation had more fat tissue ((93.60±2.40) %) and less MAC2+CD206- M1 macrophages ((0.50±0.47) cells/field) compared to control group (fat tissue (45.42±5.96) and macrophage cells/field (5.00±2.23)). Moreover, the cell tracing dye labeled hUCMSCs were confirmed to differentiate into adipocytes and vascular endothelial cells in adipose tissue. Additionally, when applied to the tissue-engineered breast with scaffold, the group treat with hUCMSCs had more adipose tissues and CD31+ cells than the control group.Conclusions: Our findings demonstrate the role of allogeneic hUCMSCs in regenerating adipose tissue and may provide a new strategy to construct tissue engineered breast.

DOSE-DEPENDENT EFFECTS OF ADIPOSE TISSUE-DERIVED STROMAL VASCULAR FRACTION CELLS ON ANGIOGENESIS AND FIBROSIS IN HUMAN FAT GRAFTS

2014 ◽  
Vol 26 (03) ◽  
pp. 1450045
Author(s):  
Shou-Cheng Teng ◽  
Li-Ting Li ◽  
Shyi-Gen Chen ◽  
Tien-Mu Chen ◽  
Cheng-Hao Liao ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Stromal Vascular Fraction ◽  
Cyst Formation ◽  
Fat Graft ◽  
Fat Tissue ◽  
Fat Grafts ◽  
Regenerative Cells ◽  
Liposuction Surgery ◽  
Dose Dependent ◽  
Anti Inflammation

Adipose tissue is not only an ideal material for soft tissue filling and augmentation, but also a plentiful source of regenerative cells in adipose-derived stromal vascular fraction. Enriched fat tissue with adipose-derived stromal vascular fraction cells (ADSVFCs) can improve the fat graft survival, as shown in previous studies. The objective of this study is to estimate the effects of various dosages of ADSVFCs on fat grafts. We hypothesized that the improvement of ADSVFCs on fat transplantation quality would be dose dependent. Fat tissues were obtained by liposuction surgery. ADSVFCs were mixed into fat tissue, and the ADSVFC-enriched fat tissues were implanted subcutaneously into nude mice. The histological findings showed that the neoangiogenesis and integrity of grafted fat cells significantly increased with increasing dosages of ADSVFCs. Higher dosages of ADSVFCs also significantly reduced the side effects of the fat grafts, including reducing inflammation, cell infiltration, fibrosis and cyst formation. In conclusion, ADSVFC supplemented fat implants can improve angiogenesis and anti-inflammation. However, these effects of ADSVFCs on fat graft quality are dose dependent.

scholarly journals Engineered Fat Graft Enhanced with Adipose-Derived Stromal Vascular Fraction Cells for Regenerative Medicine: Clinical, Histological and Instrumental Evaluation in Breast Reconstruction

2019 ◽  
Vol 8 (4) ◽  
pp. 504 ◽  
Author(s):  
Pietro Gentile ◽  
Donato Casella ◽  
Enza Palma ◽  
Claudio Calabrese
Keyword(s):  
Breast Reconstruction ◽  
Preoperative Evaluation ◽  
Three Dimensional ◽  
Stromal Vascular Fraction ◽  
Fat Graft ◽  
Control Group ◽  
Study Group ◽  
Fat Tissue ◽  
Calvarial Defects

The areas in which Stromal Vascular Fraction cells (SVFs) have been used include radiotherapy based tissue damage after mastectomy, breast augmentation, calvarial defects, Crohn’s fistulas, and damaged skeletal muscle. Currently, the authors present their experience using regenerative cell therapy in breast reconstruction. The goal of this study was to evaluate the safety and efficacy of the use of Engineered Fat Graft Enhanced with Adipose-derived Stromal Vascular Fraction cells (EF-e-A) in breast reconstruction. 121 patients that were affected by the outcomes of breast oncoplastic surgery were treated with EF-e-A, comparing the results with the control group (n = 50) treated with not enhanced fat graft (EF-ne-A). The preoperative evaluation included a complete clinical examination, a photographic assessment, biopsy, magnetic resonance (MRI) of the soft tissue, and ultrasound (US). Postoperative follow-up took place at two, seven, 15, 21, 36 weeks, and then annually. In 72.8% (n = 88) of breast reconstruction treated with EF-e-A, we observed a restoration of the breast contour and an increase of 12.8 mm in the three-dimensional volume after 12 weeks, which was only observed in 27.3% (n = 33) of patients in the control group that was treated with EF-ne-A. Transplanted fat tissue reabsorption was analyzed with instrumental MRI and US. Volumetric persistence in the study group was higher (70.8%) than that in the control group (41.4%) (p < 0.0001 vs. control group). The use of EF-e-A was safe and effective in this series of treated cases.

scholarly journals Preenrichment with Adipose Tissue-Derived Stem Cells Improves Fat Graft Retention in Patients with Contour Deformities of the Face

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Muhammad M. Bashir ◽  
Muhammad Sohail ◽  
Fridoon J. Ahmad ◽  
Mahmood S. Choudhery
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Stem Cell ◽  
Patient Satisfaction ◽  
Fat Grafting ◽  
Fat Graft ◽  
Cell Group ◽  
Patient Satisfaction Scores ◽  
The Face ◽  
Tissue Grafts

Quick absorption of adipose tissue grafts makes the outcomes less satisfactory for clinical applications. In the current study, adipose tissue grafts were mixed with adipose tissue-derived stem cells (ASCs) to improve retention of adipose tissue grafts and to make the clinical outcomes of fat grafting more reliable. Adipose tissue was either injected alone (conventional group) or mixed with ASCs (stem cell group) before injection. In both groups, adipose tissue was injected at the site of contour throughout layers of tissues till visual clinical symmetry with the opposite side was achieved. The volume of injected fat graft was measured after 72 hours and 6 months using a B-mode ultrasound device connected with a 12 MH frequency probe. The percentage reduction in the volume of injected fat, physician satisfaction scores (Ph-SCs), and patient satisfaction scores (P-SCs) were also recorded. After 6 months, there was significantly lower fat absorption in the stem cell group as compared to the conventional group. Mean physician and patient satisfaction scores were significantly improved in the stem cell group. No significant adverse effects were noted in any patient. Significantly lower absorption of graft due to the use of ASCs improves the clinical outcomes of conventional fat grafting for contour deformities of the face. The current preenrichment strategy is noninvasive, safe and can be applied to other diseases that require major tissue augmentation such as breast surgery. This trial is registered with NCT02494752.

scholarly journals Ischemia-Reperfusion Injury of Adipofascial Tissue: An Experimental Study Evaluating Early Histologic and Biochemical Alterations in Rats

2005 ◽  
Vol 2005 (5) ◽  
pp. 304-308 ◽  
Author(s):  
Yusuf Kenan Coban ◽  
Ergul Belge Kurutas ◽  
Harun Ciralik
Keyword(s):  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Control Group ◽  
Clinical Settings ◽  
Fat Tissue ◽  
Biochemical Alterations ◽  
Subcutaneous Adipose ◽  
Epigastric Flap

Fat necrosis remains a serious complication in reconstructive flaps. In clinical setting, it is well known that fat tissue is more susceptible to ischemic events. We aimed to evaluate early histological and biochemical changes of adipofascial tissue in an experimantal model. An epigastric flap model in rats was used to evaluate the effect of ischemia-reperfusion (I-R) injury on adipofascial tissue. Two groups of animals (one with ischemia alone and other ischemia-reperfusion group) were used to evaluate the degree of histological edema, congestion and extravascular bleeding, and early biochemical alterations within the adipofascial flaps. The biochemical parameters included glutathione (GSH) and malondialdehyde (MDA). In each group, contralateral groin subcutaneous adipose tissue served as control. These evaluations were compared to normal unmanipulated, contralateral abdominal subcutaneous adipose tissue. The ischemia-reperfused flap group showed histologically significantly much edema congestion and bleeding than the control groups (P<.0001). The control group showed less edema in fat tissue than the ischemia-alone group (P<.05). All of the flaps in the ischemia-only group showed significantly less bleeding and edema than I-R group (P<.001). The ratio of MDA/GSH was 33 in control, 37 in ischemia alone, and 82 in ischemia-reperfusion groups, respectively. This study confirms that significant histologic and biochemical alteration occurs after ischemia and ischemia-reperfusion events in adipose tissue. Marked drop in adipose tissue antioxidant levels after I-R suggested that preemptive measures to this decrease should be undertaken in clinical settings.

Enrichment of autologous fat grafts with ex-vivo expanded adipose tissue-derived stem cells for graft survival: a randomised placebo-controlled trial

The Lancet ◽  
2013 ◽  
Vol 382 (9898) ◽  
pp. 1113-1120 ◽  
Author(s):  
Stig-Frederik Trojahn Kølle ◽  
Anne Fischer-Nielsen ◽  
Anders Bruun Mathiasen ◽  
Jens Jørgen Elberg ◽  
Roberto S Oliveri ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Graft Survival ◽  
Ex Vivo ◽  
Controlled Trial ◽  
Autologous Fat ◽  
Fat Grafts
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