scholarly journals Improvement of the Survival of Human Autologous Fat Transplantation by Adipose-Derived Stem-Cells-Assisted Lipotransfer Combined with bFGF

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Aimei Jiang ◽  
Ming Li ◽  
Wenjing Duan ◽  
Yilong Dong ◽  
Yanmei Wang
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Histological Analysis ◽  
Angiogenic Factors ◽  
Fat Graft ◽  
Adipose Derived Stem Cells ◽  
Fat Transplantation ◽  
Autologous Fat ◽  
Fat Grafts ◽  
Autologous Fat Transplantation

Adipose-derived stem cells (ASCs) transplanted along with autologous adipose tissue may improve fat graft survival; however, the efficacy of ASCs has been diluted by low vascularization. This study was designed to test the hypothesis that basic fibroblast growth factor (bFGF) may improve the effects of ASCs because it owns the property to boost angiogenesis. In the present study, human fat tissues were mixed with ASCs, ASCs plus 100 U bFGF, or medium as the control and then injected subcutaneously into immunologically compromised nude mice for 12 weeks. Our findings demonstrated that mixture with the ASCs significantly increased the weight and volume of the fat grafts compared to control grafts, and histological analysis revealed that both ASCs and ASCs plus bFGF grafts consisted predominantly of adipose tissue and had significantly less fibrosis but greater microvascular density compared with control and also grafts mixed with ASCs had a high expression of angiogenic factors. More importantly, the bFGF treated fat grafts shown elevate in survival, vascularization, and angiogenic factors expression when compared with the grafts that received ASCs alone. These results indicated that bFGF together with ASCs can enhance the efficacy of autologous fat transplantation and increase blood vessel generation involved in the benefits from bFGF.

scholarly journals Comparative study on the effect of adipose-derived stem cells versus alpha-lipoic acid in autologus fat graft survival in adult female albino rat: histological and immune histochemical study

QJM ◽  
2020 ◽  
Vol 113 (Supplement_1) ◽  
Author(s):  
S H Ibrahim ◽  
S M M Omar ◽  
G A Ibrahim ◽  
A E Omar
Keyword(s):  
Stem Cells ◽  
Survival Rate ◽  
Lipoic Acid ◽  
Fat Graft ◽  
Control Group ◽  
Adipose Derived Stem Cells ◽  
Alpha Lipoic Acid ◽  
Inflammatory Infiltration ◽  
Autologous Fat ◽  
Fat Grafts

Abstract Background Autologous fat transfer is a popular option for soft tissue augmentation, but has a low survival rate. So, enriching the transplanted fat with stem cells (cell assisted lipotransfer) or oral administration of alpha-lipoic acid (ALA) before and after graft injection, were thought to increase the survival rate of the transplanted fat. Aim of Study Is to compare between cell-assisted lipotransfer (CAL) and ALA therapy in improvement of fat graft survival. Materials and Methods Thirty adult female albino rats were used and divided into three groups. Group I: the control group. Group II: CAL group received autologous fat mixed with adipose derived stem cells (ASCs). Group III: ALA group in which animals received oral ALA four days before fat transplantation and continued throughout experiment. Fat grafts were collected from the sites of injection after twenty-eight days. Histological and immune histochemical studies were performed. Statistical analysis was also done. Results Histological evaluation of the fat grafts from animals of control group revealed atrophy and death of adipocytes with formation of fat cysts. Severe inflammatory infiltration with giant cells formation and fibrosis were detected. Significant decrease of VEGF reaction was seen. In CAL group significant increase in number of intact adipocytes together with decrease in inflammation and fibrosis occurred. Moreover, significant increase in VEGF reaction was observed. In ALA group, significant decrease in number of intact adipocytes and VEGF reaction was detected compared with CAL group. Also, significant increase in inflammatory infiltration and fibrosis were detected compared to CAL group. Conclusions Both ASCs and ALA showed improvement in all histological parameters compared with control group. However, enrichment of fat with ASCs showed the best results.

Autologous Fat Transplantation

1997 ◽  
Vol 14 (4) ◽  
pp. 433-443 ◽  
Author(s):  
Melvin A. Shiffman
Keyword(s):  
Preliminary Analysis ◽  
Personal Communication ◽  
Review Of The Literature ◽  
Fat Transplantation ◽  
Experimental Procedure ◽  
Autologous Fat ◽  
Fat Grafts ◽  
Autologous Fat Transplantation ◽  
Practicing Physicians ◽  
Attention To Detail

A preliminary analysis of the methods that have been used and the results achieved since fat grafts were first used in 1889 is presented. Descriptions of the techniques used by several practicing physicians were collected by personal communication and a review of the literature and are recorded in this paper. Although the process of fat transplantation has not been standardized, it can no longer be considered an experimental procedure as enough surgeons have shown that there can be consistently good results. Failures and complications are not the result of the theory that fat cannot be transplanted, but the result of errors in technique and not adequate attention to detail.

Promotion of Primary Murine Breast Cancer Growth and Metastasis by Adipose-Derived Stem Cells Is Reduced in the Presence of Autologous Fat Graft

2019 ◽  
Vol 143 (1) ◽  
pp. 137-147 ◽  
Author(s):  
Simon Gebremeskel ◽  
John Gencarelli ◽  
Alison J. Gareau ◽  
Terry Levatte ◽  
Anton Dugandzic ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Stem Cells ◽  
Fat Graft ◽  
Cancer Growth ◽  
Adipose Derived Stem Cells ◽  
Autologous Fat ◽  
Autologous Fat Graft ◽  
Breast Cancer Growth

Enrichment of autologous fat grafts with ex-vivo expanded adipose tissue-derived stem cells for graft survival: a randomised placebo-controlled trial

The Lancet ◽  
2013 ◽  
Vol 382 (9898) ◽  
pp. 1113-1120 ◽  
Author(s):  
Stig-Frederik Trojahn Kølle ◽  
Anne Fischer-Nielsen ◽  
Anders Bruun Mathiasen ◽  
Jens Jørgen Elberg ◽  
Roberto S Oliveri ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Graft Survival ◽  
Ex Vivo ◽  
Controlled Trial ◽  
Autologous Fat ◽  
Fat Grafts

scholarly journals Effects of increased proliferation of human adipose tissue-derived mesenchymal stem cells by sphingosylphosphorylcholine on the survival of cryopreserved fat grafts

2018 ◽  
Vol 70 (3) ◽  
pp. 543-549
Author(s):  
Yong Bae ◽  
Ji Song ◽  
Kyeong Nam ◽  
Joo Kim ◽  
Su Nam
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Nude Mice ◽  
Soft Tissue Augmentation ◽  
Fat Graft ◽  
Saline Control ◽  
Control Group ◽  
Fat Tissue ◽  
Lipid Molecule ◽  
Fat Grafts

The use of cryopreserved adipose tissue for soft-tissue augmentation is common, but the unpredictability of fat graft viability remains a limitation. Human adipose-derived stem cells (hADSC) have been introduced to enhance viability and improve the survival of transplanted fat tissue. Sphingosylphosphorylcholine (SPC) is a bioactive lipid molecule involved in various cellular responses. SPC stimulates the proliferation of various cell types such as hADSC. We demonstrated the effects of hADSC and SPC on the survival of cryopreserved fat grafts in nude mice. The cryopreserved fat grafts were treated with hADSC or hADSC+SPC, and a normal saline (control) mixture in BALB/c male nude mice. We examined the weight and volume of the mice in each group (n=11) at 8 weeks after transplantation to evaluate the survival of the fat tissue. The hADSC group showed increased weight and volume compared with the control group. The hADSC+SPC group showed a higher survival rate in terms of weight and volume than the control or hADSC group. In addition, the hADSC+SPC treatment significantly increased the expression of angiogenic factors. These results suggest the potential clinical utility of hADSC+SPC.

scholarly journals Salvianolic acid-B improves fat graft survival by promoting proliferation and adipogenesis

2021 ◽  
Author(s):  
Jia-Ming Sun ◽  
Chia-Kang Ho ◽  
Ya Gao ◽  
Chio-Hou Chong ◽  
Dan-Ning Zheng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Graft Survival ◽  
Salvia Miltiorrhiza ◽  
Salvianolic Acid B ◽  
Fat Graft ◽  
Fat Transplantation ◽  
Salvianolic Acid ◽  
Fat Grafts

Abstract Background: Our previous study proved that Salvia miltiorrhiza could enhance fat graft survival by promoting adipogenesis. However, the effect of salvianolic acid B (Sal-B), the most abundant and bioactive water-soluble compound in Salvia miltiorrhiza, on fat graft survival has not yet been investigated.Objective: This study aims to investigate whether salvianolic acid B could improve fat graft survival and promote preadipocyte differentiation. The underlying mechanism has also been studied.Methods: In vivo, 0.2 ml of Coleman fat was transplanted into nude mice with salvianolic acid B. The grafts were evaluated by HE and IF at 2 and 4 weeks posttransplantation and by micro-CT at 4 weeks posttransplantation. In vitro, the adipogenesis and proliferative activities of salvianolic acid B were analyzed in cultured human adipose-derived stem cells (h-ADSCs) and 3T3-L1 cells to detect the mechanism by which salvianolic acid B affects graft survival.Results: In vivo, the weights and volumes of the fat grafts in the Sal-B-treated groups were significantly higher than those of the fat grafts in the control group. In addition, higher fat integrity and more viable adipocytes were observed in the Sal-B-treated groups. In vitro, salvianolic acid B showed the ability to promote 3T3-L1 and h-ADSC proliferation and adipogenesis.Conclusions: Our in vitro experiments demonstrated that salvianolic acid B can promote the proliferation of adipose stem cells and enhance the differentiation of adipose stem cells. Simultaneously, in vivo experiments showed that salvianolic acid B can improve the survival rate of fat transplantation. Therefore, our research shed light on the potential therapeutic usage of salvianolic acid B in improving the survival rate of fat transplantation.

scholarly journals Characteristics and Potentiality of Human Adipose-Derived Stem Cells (hASCs) Obtained from Enzymatic Digestion of Fat Graft

Cells ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 282 ◽  
Author(s):  
Pietro Gentile ◽  
Maria Piccinno ◽  
Claudio Calabrese
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Ex Vivo ◽  
Stromal Vascular Fraction ◽  
Enzymatic Digestion ◽  
Histological Evaluation ◽  
Fat Graft ◽  
Adipose Derived Stem Cells ◽  
Differentiation Potential ◽  
New Therapeutic Approaches

Human adipose-derived stem cells localize in the stromal-vascular portion, and can be ex vivo isolated using a combination of washing steps and enzymatic digestion. For this study, we undertook a histological evaluation of traditional fat graft compared with fat graft enriched with stromal vascular fraction cells isolated by the Celution™ system to assess the interactions between cells and adipose tissue before the breast injection. In addition, we reported on histological analyses of biopsies derived from fat grafted (traditional or enriched with SVFs) in the breast in order to assess the quality of the adipose tissue, fibrosis and vessels. The hASCs derived from enzymatic digestion were systematically characterized for growth features, phenotype and multi-potent differentiation potential. They fulfill the definition of mesenchymal stem cells, albeit with a higher neural phenotype profile. These cells also express genes that constitute the core circuitry of self-renewal such as OCT4, SOX2, NANOG and neurogenic lineage genes such as NEUROD1, PAX6 and SOX3. Such findings support the hypothesis that hASCs may have a potential usefulness in neurodegenerative conditions. These data can be helpful for the development of new therapeutic approaches in personalized medicine to assess safety and efficacy of the breast reconstruction.

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