scholarly journals Anatomical and micromorphological characteristics of the seed coat of field pea (Pisum sativum L.) genotypes in relation to cracks and damage of seeds

2017 ◽  
Vol 69 (3) ◽  
pp. 503-512 ◽  
Author(s):  
Jelena Lazarevic ◽  
Lana Zoric ◽  
Djura Karagic ◽  
Branko Milosevic ◽  
Dunja Karanovic ◽  
...  

In this paper, we present the morphological characteristics of the seed and micromorphological, anatomical and chemical characteristics of the seed coat of pea (Pisum sativum L.) genotypes, Jezero, Javor and NS Junior. Our aim was to investigate whether these genotypes can be differentiated based on seed coat morphoanatomical characteristics, depending on the harvest treatment. The observations and measurements of seed coat cross-sections were performed using light microscopy. The seed coat surface was observed using SEM. A tuberculate seed coat surface characterized all examined pea genotypes, and the average diameter of the tubercle was about 12 ?m. Statistical and laboratory analyses revealed that major damage was the most frequent defect type as the result of mechanized harvest in all the examined genotypes. Genotype NS Junior had the shortest seed length (6.1 mm). Micromorphological analysis revealed that the seed surface was tuberculate in all genotypes. The genotype Jezero had the highest number of tubercle ribs (11.0) and a significantly higher proportion of parenchyma tissue (50.6%), while NS Junior was characterized by the greatest share of macrosclereids (49.8%). The highest number of osteosclereids (832/mm2) was counted in genotype Javor. In addition, genotype NS Junior stands out due to the highest percentage of crude fiber (62.75 g/100g) in the seed coat. There was a marked difference among the studied genotypes with regard to the seed coat morphoanatomical characteristics, which is confirmed by the results of multivariate discriminant analysis (MDA). These results suggested that the morphological, micromorphological and anatomical characteristics of the seed might have an impact on the seed coat damage level at harvest.

2009 ◽  
Vol 89 (1) ◽  
pp. 73-75 ◽  
Author(s):  
Deng-Jin Bing ◽  
Don Beauchesne ◽  
Al Sloan ◽  
Yantai Gan ◽  
Cecil Vera ◽  
...  

Hugo is a high-yielding field pea (Pisum sativum L.) cultivar with yellow cotyledons. It has a semi-leafless leaf type, and is powdery mildew resistant. It has round seed shape, medium seed size and high seed coat integrity. The cultivar is adapted to field pea growing regions in western Canada. Key words: Field pea, Pisum sativum, cultivar description


2020 ◽  
Vol 50 (5) ◽  
Author(s):  
Tianyao Zhao ◽  
Wenjin Su ◽  
Yang Qin ◽  
Liyun Wang ◽  
Yufan Kang

ABSTRACT: Pea (Pisum sativum L.) is the fourth leading legume crop in the world, and its demand is increasing. In this study, the morphological characteristics (seed shape, seed surface, seed coat color, hilum color, cotyledon color, 100-seed weight and color values), total phenolic content (TPC), total flavonoid content (TFC), 2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) free radical scavenging capacity and ferric reducing antioxidant power (FRAP) of 75 pea cultivars were investigated. Results showed rich genetic diversity and a wide range of phenolic contents and antioxidant activities. Sixteen varieties enriched with phenolic contents and high antioxidant activities were screened out. A significant correlation was reported among color values, TPC, TFC, ABTS and FRAP. Principal component analysis (PCA) extracted four principal components with a total cumulative contribution of 81.29%. Hierarchical cluster analysis based on the four extracted principal components resulted in a dendrogram dividing the peas into three groups. In addition, dark pea seeds have potential as a functional food in addition to their traditional role in providing dietary protein and fibre. This study provided a scientific basis for the breeding of pea varieties, development of new products and improvement of pea resource utilization.


2019 ◽  
Vol 31 (1) ◽  
pp. 101-116
Author(s):  
Fatih Hanci

AbstractThe aim of this study was to identify the molecular and morphological characteristics of Turkish pea accessions (Pisum sativum L.). The genetic diversity among 130 Turkish landraces and 2 commercial varieties in a total of 132 pea accessions was assessed with 14 simple sequence repeat (SSR) markers. Forty-eight (48) polymorphic alleles were identified using 14 SSR markers. The pairwise Dice coefficients of similarity between accessions ranged from 0.091 to 0.960. The polymorphism information content (PIC) value ranged from 0.585 to 0.861. Overall, 50 morphological traits were evaluated. Cluster analysis was carried out on a matrix of Euclidean distances. The accessions were divided into three main groups. Principal component analysis (PCA) was used to identify the weight of each morphological characteristic. According to the results, the highest eigenvalue was observed in PC-I (13.88) followed by PC-II (11.42), and PC-III (7.32). The first fifteen PCs with eigenvalues > 1 explained 74.08% of the variability. The results showed that the molecular markers were useful and polymorphic, sufficient to allocate all the evaluated accessions. This research has provided significant insights into the genetic variability of Turkish pea accessions.


2019 ◽  
Vol 13 (2) ◽  
pp. 208-218
Author(s):  
Juan Diego Duque-Zapata ◽  
Jaime Eduardo Muñoz ◽  
Oscar Checa-Coral

The pea (Pisum sativum L.) is one of the more important legume crops produced globally. We studied the structure and genetic diversity in a collection of 50 pea accessions with 16 simple sequence repeat (SSR) markers, whose average polymorphic information content (PIC) was 0.62. The SSR markers amplified a total of 28 alleles with an average of 4 alleles per locus, with locus AB71 and D21 amplifying the largest number of alleles (6). The observed heterozygosity (Ho) was 0.09±0.08 and the expected heterozygosity (He) was 0.42, indicating an elevated level of inbreeding (Fis = 0.60). The genetic relationships were inferred with a similarity index (DICE) and a bayesian analysis (STRUCTURE), detecting 2 clusters for the genotypes, with a high similarity of the morphological characteristics of each genotype. The results of this study will be useful for the creation of future breeding programs.The pea (Pisum sativum L.) is one of the more important legume crops produced globally. We studied the structure and genetic diversity in a collection of 50 pea accessions with 16 simple sequence repeat (SSR) markers, whose average polymorphic information content (PIC) was 0.62. The SSR markers amplified a total of 28 alleles with an average of 4 alleles per locus, with locus AB71 and D21 amplifying the largest number of alleles (6). The observed heterozygosity (Ho) was 0.09±0.08 and the expected heterozygosity (He) was 0.42, indicating an elevated level of inbreeding (Fis = 0.60). The genetic relationships were inferred with a similarity index (DICE) and a bayesian analysis (STRUCTURE), detecting 2 clusters for the genotypes, with a high similarity of the morphological characteristics of each genotype. The results of this study will be useful for the creation of future breeding programs.


1987 ◽  
Vol 67 (3) ◽  
pp. 667-674 ◽  
Author(s):  
R. D. REICHERT ◽  
A. O. F. EHIWE

Seed coat breakage of field pea (Pisum sativum L.) during harvesting and handling increases dockage and reduces seed quality. A laboratory-scale abrasive dehulling device was used to evaluate seed coat durability of field peas grown in 1983 (20 cultivars, 6 locations) and 1984 (17 cultivars, 5 locations). Analysis of variance revealed significant (P < 0.05) effects due to cultivar and location of growth for both years. Mean cultivar seed coat breakage ranged from 1.5 to 22.3% in 1983 and from 0.6 to 14.9% in 1984. The seed coat breakage of several cultivars in 1983 (MP 913, Line 32, Tipu, MP 926 and MP 942) and 1984 (MP 939 and Sv20565) was greater (P < 0.05) than that of Century, the current standard in the industry. The seed coat breakage of Bellevue was less (P < 0.05) than that of Century in both years. Locations which experienced hot or dry fall conditions produced seed with poor seed coat durability. Broad sense heritability of seed coat durability, based on the components of variance method, was 57.5 and 56.2% in 1983 and 1984, respectively. Seed coat breakage was not correlated (P > 0.05) with seed coat content or thickness, protein, lignin, neutral detergent fiber, hemicellulose content, 1000-seed weight or strength of adhesion between the cotyledons. Cellulose content of the seed coat was negatively correlated (r = −0.77, P < 0.05) with seed coat breakage in 1984, but not in 1983 (P > 0.05).Key words: Pea, Pisum sativum L., seed coat durability


LWT ◽  
2002 ◽  
Vol 35 (2) ◽  
pp. 158-164 ◽  
Author(s):  
Agnieszka Troszyńska ◽  
Isabel Estrella ◽  
M.Luisa López-Amóres ◽  
Teresa Hernández

Planta ◽  
1997 ◽  
Vol 201 (2) ◽  
pp. 128-137 ◽  
Author(s):  
A. D�jardin ◽  
C. Rochat ◽  
S. Maugenest ◽  
J. -P. Boutin

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