scholarly journals Antioxidant activity of pea protein hydrolysates produced by batch fermentation with lactic acid bacteria

2015 ◽  
Vol 67 (3) ◽  
pp. 1033-1042 ◽  
Author(s):  
Nemanja Stanisavljevic ◽  
Goran Vukotic ◽  
Ferenc Pastor ◽  
Desanka Suznjevic ◽  
Zivko Jovanovic ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Large Scale ◽  
Lactobacillus Rhamnosus ◽  
Weight Fraction ◽  
Exchange Chromatography ◽  
Scale Production ◽  
Large Scale Production ◽  
Pea Proteins ◽  
Basic Peptides ◽  
Pea Seed

Nine Lactobacillus strains known for surface proteinase activity were chosen from our collection and tested for their ability to grow in pea seed protein-based medium, and to hydrolyze purified pea proteins in order to produce peptides with antioxidant (AO) activity. Two strains, Lactobacillus rhamnosus BGT10 and Lactobacillus zeae LMG17315, exhibited strong proteolytic activity against pea proteins. The AO activity of the pea hydrolysate fraction, MW <10 kDa, obtained by the fermentation of purified pea proteins with Lactobacillus rhamnosus BGT10, was tested by standard spectrophotometric assays (DPPH, ABTS, Fe3+-reducing capacity) and the recently developed direct current (DC) polarographic assay. The low molecular weight fraction of the obtained hydrolysate was separated using ion exchange chromatography, while the AO activity of eluted fractions was determined by means of a sensitive DC polarographic assay without previous concentration of samples. Results revealed that the fraction present in low abundance that contained basic peptides possessed the highest antioxidant activity. Based on the obtained results, it can be concluded that Lactobacillus rhamnosus BGT10 should be further investigated as a candidate strain for large-scale production of bioactive peptides from legume proteins.

scholarly journals Large-scale Production of Novel Porcine Circovirus Type 2d (PCV2d) Subunit Vaccine Using E. coli Platform

2021 ◽  
Author(s):  
Sarawuth Noppiboon ◽  
Thunyaluk Kerdboon ◽  
Neeracha Lapanusorn ◽  
Pisit Ekkpongpaisit ◽  
Sarah Slack ◽  
...  
Keyword(s):  
Large Scale ◽  
Pilot Scale ◽  
Porcine Circovirus Type ◽  
Exchange Chromatography ◽  
Porcine Circovirus ◽  
Scale Production ◽  
E Coli ◽  
Large Scale Production ◽  
Production Platform ◽  
Cost Efficient

Porcine Circovirus type 2d (PCV2d) is becoming the predominant PCV genotype and considerably affects the global pig industry. Nevertheless, currently no commercial PCV2d vaccine is available. Preventing and controlling the disease caused by PCV2d is therefore based on other genotype-based vaccines. However, their production platforms are laborious, limited in expression level and relatively expensive for veterinary applications. To address these challenges, we have developed a simple and cost-efficient platform for a novel PCV2d vaccine production platform, using fed-batch E. coli fermentation followed by cell disruption and filtration, and a single purification step via cation exchange chromatography. The process was developed at bench scale and then pilot scale where the PCV2d subunit protein yield was approximately 0.8 g/L fermentation volume in a short production time. Moreover, we have successfully implemented this production process at two different sites, in Southeast Asia and Europe. This demonstrates transferability and the high potential for successful industrial production.

scholarly journals Cytotoxic and Antioxidant Activity of Zinc Oxide Nanoparticles Synthesised Using Maranta Arundinacea Root Extract

2020 ◽  
Vol 11 (3) ◽  
pp. 4372-4377
Author(s):  
Aparna J ◽  
Rajeshkumar S
Keyword(s):  
Antioxidant Activity ◽  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Large Scale ◽  
Oxide Nanoparticles ◽  
Root Extract ◽  
Scale Production ◽  
Synthesis Of Nanoparticles ◽  
Large Scale Production ◽  
Uv Visible

The main aim of this study was to employ Maranta arundinacea root extract in the synthesis of Zinc oxide nanoparticles and check the antioxidant activity and cytotoxic effect. The use of nanotechnology in medicine especially for drug delivery is shown to have numerous benefits. Nanoparticles are being used to lessen toxicity and facet results that capsules may additionally impose on the patient. The zinc oxide nanoparticles synthesized using different plant extract used for many biomedical applications such as anticancer activity, antibacterial activity, antioxidant activity, antifungal activity and many more applications used in medicine ( Diagnosis and drug) Plant mediated organic synthesis of nanoparticles has been gaining importance because of its simplicity and eco friendliness. Maranta arundinacea mediated Zinc oxide nanoparticles were initially synthesised and characterised by UV-Visible spectrophotometer and TEM. Further the Maranta arundinacea mediated ZnoNPs were subjected to DPPH assay to determine the antioxidant activity. Maranta arundinacea mediated ZnoNPs were biosynthesised with ease and showed good antioxidant and cytotoxic activity . This study concludes that Maranta arundinacea mediated ZnoNPs nanoparticles have the potential to be used as an effective antioxidant. Hence, it may be employed in large scale production and may be used in many medicinal applications where there is a need for antioxidant.

scholarly journals A Novel Method for High-Level Production of TEV Protease by Superfolder GFP Tag

2009 ◽  
Vol 2009 ◽  
pp. 1-8 ◽  
Author(s):  
Xudong Wu ◽  
Di Wu ◽  
Zhisheng Lu ◽  
Wentao Chen ◽  
Xiaojian Hu ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Large Scale ◽  
Fluorescent Protein ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Scale Production ◽  
Sequence Specificity ◽  
Tev Protease ◽  
Large Scale Production ◽  
Novel Method

Because of its stringent sequence specificity, tobacco etch virus (TEV) protease is widely used to remove fusion tags from recombinant proteins. Due to the poor solubility of TEV protease, many strategies have been employed to increase the expression level of this enzyme. In our work, we introduced a novel method to produce TEV protease by using visible superfolder green fluorescent protein (sfGFP) as the fusion tag. The soluble production and catalytic activity of six variants ofsfGFP-TEV was examined, and then the best variant was selected for large-scale production. After purified by Ni-NTA affinity chromatography and Q anion exchange chromatography, the best variant ofsfGFP-TEV fusion protease was obtained with purity of over 98% and yield of over 320 mg per liter culture. ThesfGFP-TEV had a similar catalytic activity to that of the original TEV protease. Our research showed a novel method of large-scale production of visible and functional TEV protease for structural genomics research and other applications.

scholarly journals Selection and characterization of a SpaCBA pilus-secreting food-grade derivative of Lacticaseibacillus rhamnosus GG

2021 ◽  
Author(s):  
Hanne L. P. Tytgat ◽  
Pia Rasinkangas ◽  
Jarmo Ritari ◽  
Justus Reunanen ◽  
Steven Aalvink ◽  
...  
Keyword(s):  
Large Scale ◽  
Low Cost ◽  
Lactobacillus Rhamnosus ◽  
Scale Production ◽  
Therapeutic Application ◽  
Food Grade ◽  
Large Scale Production ◽  
Key Points

AbstractMany studies have established the functional properties of Lacticaseibacillus rhamnosus GG, previously known as Lactobacillus rhamnosus GG, marketed worldwide as a probiotic. The extraordinary capacity of L. rhamnosus GG to bind to human mucus and influence the immune system especially stand out. Earlier, we have shown the key role of its SpaCBA sortase-dependent pili encoded by the spaCBA-srtC1 gene cluster herein. These heterotrimeric pili consist of a shaft pilin SpaA, a basal pilin SpaB, and tip pilin SpaC that contains a mucus-binding domain. Here, we set out to characterize a food-grade non-GMO mutant of L. rhamnosus GG, strain PA11, which secretes its pilins, rather than coupling them to the cell surface, due to a defect in the housekeeping sortase A. The sortase-negative strain PA11 was extensively characterized using functional genomics and biochemical approaches and found to secrete the SpaCBA pili into the supernatant. Given the functional importance and uniqueness of the mucus-binding pili of L. rhamnosus GG, strain PA11 offers novel opportunities towards the characterization and further therapeutic application of SpaCBA pili and their low-cost, large-scale production. Key points •Creation of pilus-secreting mutant (PA11) of the key probiotic LGG. •Strain PA11 is defective in a functional housekeeping sortase SrtA. •Strain PA11 opens novel biotherapeutic application avenues. Graphical abstract

scholarly journals Saccharomyces Cerevisiae Var. Boulardii: Valuable Probiotic Starter for Craft Beer Production

2019 ◽  
Vol 9 (16) ◽  
pp. 3250 ◽  
Author(s):  
Joaquín Mulero-Cerezo ◽  
Álvaro Briz-Redón ◽  
Ángel Serrano-Aroca
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Antioxidant Activity ◽  
Large Scale ◽  
Bioreactor Culture ◽  
Scale Production ◽  
Craft Beer ◽  
Yeast Viability ◽  
Large Scale Production ◽  
Lower Alcohol ◽  
Probiotic Yeast

The use of probiotic starters remaining viable in unpasteurized and unfiltered beers could significantly increase health benefits. Here, the probiotic Saccharomyces cerevisiae var. boulardii (Scb) and a commercial Saccharomyces cerevisiae (Sc) strain, which is commonly employed in the brewing industry, are compared as single starters. The healthy value of the produced beers and growth performance in a laboratory bioreactor are analysed by determining antioxidant activity, phenolic content and profile, alcohol, biomass growth modelling by the logistic and Gompertz equations, biovolume estimation from 2D microscopy images, and yeast viability after fermentation. Thus, in this study, the craft beer produced with the probiotic yeast possessed higher antioxidant activity, lower alcohol content, similar sensory attributes, much higher yeast viability and more acidification, which is very desirable to reduce contamination risks at large-scale production. Furthermore, Scb exhibited faster growth in the bioreactor culture and larger cell volumes than Sc, which increases the probiotic volume of the final craft beer.

scholarly journals Production and Partial Purification of Heat-Stable Enterotoxin (A) Produced by Enterotoxigenic Escherichia coli

2010 ◽  
Vol 34 (1) ◽  
pp. 122-129
Author(s):  
Rajiha I. Al-Nuaimy
Keyword(s):  
Large Scale ◽  
Specific Activity ◽  
Exchange Chromatography ◽  
Enterotoxigenic Escherichia Coli ◽  
Partial Purification ◽  
Scale Production ◽  
E Coli ◽  
Standard Curve ◽  
Heat Stable ◽  
Large Scale Production

A total of (25) stool samples were collected from children and adults (2- 4) years oldsuffering from diarrhea to isolate E. coli strains that produce heat-stable enterotoxin a (STa),and after performing microscopic examination, cultural characterization and biochemicalidentification only (11) isolates showed positive E. coli. STa activity was estimated by usingsuckling mouse assay (SMA) and from these (11) isolates only (5) showed STa activity andthe one with the highest STa activity was selected for large scale production of STa, whichwas followed by partial purification using ion-exchange chromatography (normal phase)using DEAE sephadex A-50 column. After purification and determination of proteinconcentration by using the standard curve of bovine serum albumin, the concentration oftoxin-protein was estimated as (1.08) mg/ml. The specific activity varied from (350) U/mgprotein at the first step of purification to (2366.6) U/mg protein at the final step, while thefinal purification of the toxin was about (6.76) fold and with a yield of (18.25) %.

I. S. A. Baud. Forms of Production and Women's Labour: Gender Aspects of Industrialisation in India and Mexico. New Delhi: Sage Publications. 1992. 321 pp.Hardbound. Price: Indian Rs 295.00.

1993 ◽  
Vol 32 (1) ◽  
pp. 129-131
Author(s):  
Naureen Talha
Keyword(s):  
Large Scale ◽  
Indian Society ◽  
New Delhi ◽  
Scale Production ◽  
Female Labour ◽  
Self Employment ◽  
Commodity Production ◽  
Large Scale Production ◽  
Mexican Society ◽  
Small Production

The literature on female labour in Third World countries has become quite extensive. India, being comparatively more advanced industrially, and in view of its size and population, presents a pictures of multiplicity of problems which face the female labour market. However, the author has also included Mexico in this analytical study. It is interesting to see the characteristics of developing industrialisation in two different societies: the Indian society, which is conservative, and the Mexican society, which is progressive. In the first chapter of the book, the author explains that he is not concerned with the process of industrialisation and female labour employed at different levels of work, but that he is interested in forms of production and women's employment in large-scale production, petty commodity production, marginal small production, and self-employment in the informal sector. It is only by analysis of these forms that the picture of females having a lower status is understood in its social and political setting.

An Efficient and Improved Process for the Synthesis of Itopride Hydrochloride and Trimethobenzamide Hydrochloride

2018 ◽  
Vol 15 (4) ◽  
pp. 572-575 ◽  
Author(s):  
Ponnusamy Kannan ◽  
Samuel I.D. Presley ◽  
Pallikondaperumal Shanmugasundaram ◽  
Nagapillai Prakash ◽  
Deivanayagam Easwaramoorthy
Keyword(s):  
Large Scale ◽  
Hydroxylamine Hydrochloride ◽  
Scale Production ◽  
One Pot ◽  
Hydrochloride Salt ◽  
Environmental Friendly ◽  
Large Scale Production ◽  
Non Ulcer Dyspepsia ◽  
Itopride Hydrochloride ◽  
Yield And Purity

Aim and Objective: Itopride is a prokinetic agent used for treating conditions like non-ulcer dyspepsia. Itopride is administered as its hydrochloride salt. Trimethobenzamide is used for treating nausea and vomiting and administered as its hydrochloride salt. The aim is to develop a novel and environmental friendly method for large-scale production of itopride and trimethobenzamide. Materials and Methods: Itopride and trimethobenzamide can be prepared from a common intermediate 4- (dimethylaminoethoxy) benzyl amine. The intermediate is prepared from one pot synthesis using Phyrdroxybenzaldehye and zinc dust and further reaction of the intermediate with substituted methoxy benzoic acid along with boric acid and PEG gives itopride and trimethobenzamide. Results: The intermediate 4-(dimethylaminoethoxy) benzylamine is prepared by treating p-hydroxybenzaldehyde and 2-dimethylaminoethyl chloride. The aldehyde formed is treated with hydroxylamine hydrochloride. The intermediate is confirmed by NMR and the purity is analysed by HPLC. Conclusion: Both itopride and trimethobenzamide were successfully synthesized by this method. The developed method is environmental friendly, economical for large-scale production with good yield and purity.

scholarly journals Cyanobacteria—From the Oceans to the Potential Biotechnological and Biomedical Applications

Marine Drugs ◽  
2021 ◽  
Vol 19 (5) ◽  
pp. 241
Author(s):  
Shaden A. M. Khalifa ◽  
Eslam S. Shedid ◽  
Essa M. Saied ◽  
Amir Reza Jassbi ◽  
Fatemeh H. Jamebozorgi ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Biomedical Applications ◽  
Large Scale ◽  
Biological Activities ◽  
Scale Production ◽  
Pharmacological Activities ◽  
Large Scale Production ◽  
Marine Products ◽  
Hiv 1 ◽  
Successful Phase

Cyanobacteria are photosynthetic prokaryotic organisms which represent a significant source of novel, bioactive, secondary metabolites, and they are also considered an abundant source of bioactive compounds/drugs, such as dolastatin, cryptophycin 1, curacin toyocamycin, phytoalexin, cyanovirin-N and phycocyanin. Some of these compounds have displayed promising results in successful Phase I, II, III and IV clinical trials. Additionally, the cyanobacterial compounds applied to medical research have demonstrated an exciting future with great potential to be developed into new medicines. Most of these compounds have exhibited strong pharmacological activities, including neurotoxicity, cytotoxicity and antiviral activity against HCMV, HSV-1, HHV-6 and HIV-1, so these metabolites could be promising candidates for COVID-19 treatment. Therefore, the effective large-scale production of natural marine products through synthesis is important for resolving the existing issues associated with chemical isolation, including small yields, and may be necessary to better investigate their biological activities. Herein, we highlight the total synthesized and stereochemical determinations of the cyanobacterial bioactive compounds. Furthermore, this review primarily focuses on the biotechnological applications of cyanobacteria, including applications as cosmetics, food supplements, and the nanobiotechnological applications of cyanobacterial bioactive compounds in potential medicinal applications for various human diseases are discussed.

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