scholarly journals Investigation into the flux distribution of central carbon metabolism in Corynebacterium glutamicum using principal component analysis

2015 ◽  
Vol 67 (3) ◽  
pp. 767-774
Author(s):  
Chuanyu Shang ◽  
Xiangfei Zhou ◽  
Wenwei Zhou ◽  
Xiaoyao Xie ◽  
Yin Yi
Keyword(s):  
Corynebacterium Glutamicum ◽  
Carbon Metabolism ◽  
Flux Distribution ◽  
Environmental Changes ◽  
Principal Component ◽  
Central Carbon Metabolism ◽  
Preliminary Examination ◽  
Genetic Modifications ◽  
Central Carbon

Central carbon metabolism is the main source of energy required by organisms and it provides precursors for other in vivo metabolic processes. The flux flowing through the pathways involved in central carbon metabolism characterizes its biological function and genetic readout between species or environments. In recent years, using a 13C tracer technique, researchers have measured the flux of central carbon metabolism in Corynebacterium glutamicum under a variety of nutritional and environmental changes or genetic modifications. However, there is no integrated and comparative analysis of these measured flux values. In this study, the flux values of central carbon metabolism in Corynebacterium glutamicum that were obtained in other recent studies were consolidated. A preliminary examination of the distribution characteristics of flux values in each metabolic pathway was conducted and the regression relationship between different fluxes was investigated. The principal components of the flux vector were further extracted and aggregated based on the components, and the general features of flux distribution of central carbon metabolism as well as the influence of environmental and genetic factors on the flux distribution were determined. This study provides a foundation for further investigation into the flux distribution and regulation characteristics of central carbon metabolism.

scholarly journals Ex vivo and in vivo stable isotope labelling of central carbon metabolism and related pathways with analysis by LC–MS/MS

2019 ◽  
Vol 14 (2) ◽  
pp. 313-330 ◽  
Author(s):  
Min Yuan ◽  
Daniel M. Kremer ◽  
He Huang ◽  
Susanne B. Breitkopf ◽  
Issam Ben-Sahra ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Carbon Metabolism ◽  
Ex Vivo ◽  
Central Carbon Metabolism ◽  
Stable Isotope Labelling ◽  
Isotope Labelling ◽  
Central Carbon

scholarly journals Modulation of the central carbon metabolism of Corynebacterium glutamicum improves malonyl‐CoA availability and increases plant polyphenol synthesis

2019 ◽  
Vol 116 (6) ◽  
pp. 1380-1391 ◽  
Author(s):  
Lars Milke ◽  
Patrícia Ferreira ◽  
Nicolai Kallscheuer ◽  
Adelaide Braga ◽  
Michael Vogt ◽  
...  
Keyword(s):  
Corynebacterium Glutamicum ◽  
Carbon Metabolism ◽  
Central Carbon Metabolism ◽  
Malonyl Coa ◽  
Plant Polyphenol ◽  
Central Carbon

Central carbon metabolism of Leishmania parasites

Parasitology ◽  
2010 ◽  
Vol 137 (9) ◽  
pp. 1303-1313 ◽  
Author(s):  
ELEANOR C. SAUNDERS ◽  
DAVID P. DE SOUZA ◽  
THOMAS NADERER ◽  
MARIJKE F. SERNEE ◽  
JULIE E. RALTON ◽  
...  
Keyword(s):  
Carbon Metabolism ◽  
Drug Targets ◽  
Carbon Sources ◽  
Central Carbon Metabolism ◽  
Mammalian Host ◽  
Insect Vector ◽  
Carbon Utilization ◽  
Central Carbon ◽  
Host Infection

SUMMARYLeishmania spp. are sandfly-transmitted protozoa parasites that cause a spectrum of diseases in humans. Many enzymes involved in Leishmania central carbon metabolism differ from their equivalents in the mammalian host and are potential drug targets. In this review we summarize recent advances in our understanding of Leishmania central carbon metabolism, focusing on pathways of carbon utilization that are required for growth and pathogenesis in the mammalian host. While Leishmania central carbon metabolism shares many features in common with other pathogenic trypanosomatids, significant differences are also apparent. Leishmania parasites are also unusual in constitutively expressing most core metabolic pathways throughout their life cycle, a feature that may allow these parasites to exploit a range of different carbon sources (primarily sugars and amino acids) rapidly in both the insect vector and vertebrate host. Indeed, recent gene deletion studies suggest that mammal-infective stages are dependent on multiple carbon sources in vivo. The application of metabolomic approaches, outlined here, are likely to be important in defining aspects of central carbon metabolism that are essential at different stages of mammalian host infection.

Lighting Up Live-Cell and In Vivo Central Carbon Metabolism with Genetically Encoded Fluorescent Sensors

2020 ◽  
Vol 13 (1) ◽  
pp. 293-314 ◽  
Author(s):  
Zhuo Zhang ◽  
Xiawei Cheng ◽  
Yuzheng Zhao ◽  
Yi Yang
Keyword(s):  
Carbon Metabolism ◽  
Tricarboxylic Acid Cycle ◽  
Cell Metabolism ◽  
Central Carbon Metabolism ◽  
Fluorescent Sensors ◽  
Pentose Phosphate ◽  
Spatiotemporal Information ◽  
Extant Species ◽  
Central Carbon

As the core component of cell metabolism, central carbon metabolism, consisting of glycolysis, the pentose phosphate pathway, and the tricarboxylic acid cycle converts nutrients into metabolic precursors for biomass and energy to sustain the life of virtually all extant species. The metabolite levels or distributions in central carbon metabolism often change dynamically with cell fates, development, and disease progression. However, traditional biochemical methods require cell lysis, making it challenging to obtain spatiotemporal information about metabolites in living cells and in vivo. Genetically encoded fluorescent sensors allow the rapid, sensitive, specific, and real-time readout of metabolite dynamics in living organisms, thereby offering the potential to fill the gap in current techniques. In this review, we introduce recent progress made in the development of genetically encoded fluorescent sensors for central carbon metabolism and discuss their advantages, disadvantages, and applications. Moreover, several future directions of metabolite sensors are also proposed.

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