scholarly journals Anatomy and trichome micromorphology of Stachys scardica (Griseb.) Hayek (Lamiaceae)

2014 ◽  
Vol 66 (3) ◽  
pp. 1217-1226 ◽  
Author(s):  
Slavica Grujic ◽  
Sonja Duletic-Lausevic ◽  
Ana Dzamic ◽  
P.D. Marin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Anatomical Structure ◽  
Vegetative Organs ◽  
Anatomical Features ◽  
Leaf Petiole ◽  
Plant Parts ◽  
Peltate Trichomes ◽  
Stem Leaf ◽  
Scanning Electron

The anatomy and micromorphology of the vegetative organs and calyx of Stachys scardica (Griseb.) Hayek were investigated using light (LM) and scanning electron microscopy (SEM). The analysis of the anatomical structure of stem, leaf and leaf petiole showed the presence of an additional adaxial phloem in the vascular bundle of the petiole that was recorded exclusively in species belonging to the subgenus Betonica. On the surface of studied plant parts, three types of trichomes were found: simple nonglandular uniseriate multicellular, nonglandular branched elongated and glandular peltate trichomes. The present study shows that certain micromorphological and anatomical features of Stachys taxa are valuable taxonomic characters.

scholarly journals Morphological and anatomical structure of cypselas of species of the genus Olgaea (Asteraceae: Cardueae)

Turczaninowia ◽  
2021 ◽  
Vol 24 (4) ◽  
pp. 23-46
Author(s):  
Elena V. Novozhilova ◽  
Elvira V. Boyko
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Comparative Analysis ◽  
Anatomical Structure ◽  
Anatomical Features ◽  
The Family ◽  
Scanning Electron ◽  
Shape And Size

This paper is a continuation of a series of our investigation of the morphological and anatomical structure of the cypselas of the family Asteraceae. The article presents the results of an investigation of the morphological and anatomical structure of the cypselas of 6 species of the genus Olgaea Iljin of the tribe Cardueae Cass. (O. baldschuanica (C. Winkl.) Iljin, O. leucophylla (Turcz.) Iljin, O. lomonossowii (Trautv.) Iljin, O. nidulans (Rupr.) Iljin, O. pectinata Iljin, O. tangutica Iljin) by light and scanning electron microscopy (SEM). The carpological examination of the cypselas revealed specific features of the species of the genus Olgaea: the shape of the cypsela, the sculpture of the surface of the pericarp, the absence (or presence) crowns and carpopodium, the ratio of the thickness of the pericarp and exotesta, the number of rows and the nature of thickening of the walls of mesocarp cells, the presence or absence of endocarp, the shape and size of exotesta cells, the coefficient of their palisade (the ratio of the length of the radial walls to the length of the tangent). Cypsela of Olgaea species have significant differences, which indicates the heterogeneity of the genus and the need for its revision. Based on the data obtained and the previously published results of the study (Novozhilova, Boyko, 2019), a comparative analysis of the morphological and anatomical features of the cypsela of representatives of the genera Olgaea and Alfredia Cass. was carried out, which revealed the main differences in the structure of the cypsela of these two genera. It was found that the cypsela Olgaea and Alfredia have different structures, and therefore it is inappropriate to combine them into one genus.

scholarly journals ELECTRON MICROSCOPIC STUDIES OF SOME ANTICANCER PLANTS POLLEN GRAINS

2021 ◽  
Vol 9 (10) ◽  
pp. 1386-1393
Author(s):  
Jayshree Sandesh Thaware ◽  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Morphological Characters ◽  
Electron Microscopic ◽  
Plant Parts ◽  
Morphological Studies ◽  
Datura Metel ◽  
Scanning Electron

Pollen is appropriately referred by some as Golden dust extremely valuable on account of their tremendous applications in science, industries and public health. No other plant part even though extremely tiny in size is packed with so much information and power. Similar to other plant parts, pollen characters are so varied that the classification system of plants can be built up entirely on the basis of pollen morphology.Palynology is the distinct branch of biology that deals with the dispersed microscopic tiny living and fossil entities including pollen grains, spores, algal and fungal fragments and others. An important aspect of Palynology is the Pollen morphology. The importance of Palynology in taxonomic and phylogenetic consideration of plants is well known. The changes occurring through hybridization and years of cultivation are reflected in pollen morphology. The scope and interest in the study of pollen morphology have widened with the advent of Scanning Electron Microscopy (SEM) and with regards to unipalynous taxa particularly the understanding of finer morphology is of fundamental importance. SEM gives a correct understanding of exine surface as the electron photographs of the surface replica of the exine provides the exact picture of the ornamentation pattern. The variation in the pollen morphological characters helps in the classification of plant taxa and their assessment of their phylogenetic relationship. In the present investigation, the pollen morphological studies were carried out of some ethnomedicinal plants like Catharanthus roseus, Allamanda cathartica, Datura metel, Brassica juncea, Raphanus sativus and Cleome viscosa pollen grains by Scanning electron microscopy. All that they possess anticancer characteristics in common.

Simplified Procedure for Hand Fracturing, Identifying, and Curating Small Macrocharcoal Remains

IAWA Journal ◽  
2010 ◽  
Vol 31 (2) ◽  
pp. 139-147 ◽  
Author(s):  
Jeffrey R. Boutain ◽  
Adam R. Brown ◽  
David T. Webb ◽  
Bryson H. Toyofuku
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Maximum Amount ◽  
Anatomical Structure ◽  
Alternative Procedure ◽  
Evacuation Time ◽  
Amount Of Information ◽  
Plant Remains ◽  
Simplified Procedure ◽  
Scanning Electron

Charred plant remains are common and significant components of many archeological assemblages, and the proper identification of these remains is essential for an excavation team to gather the maximum amount of information. Identification of charred plant remains, especially of small pieces, can be difficult due to the brittle characteristics of charcoal and changes in anatomical structure due to charring. Charcoal must be snapped, which is difficult for small specimens, or sectioned with time consuming resin embedding procedures. This study presents an alternative procedure in which small (0.7 mm thick) charcoal specimens are produced, attached to specimen mounting stubs used in scanning electron microscopy (SEM), and then hand snapped. This procedure consistently produced flat viewing surfaces. It also reduced the air evacuation time in SEM and facilitated the production of replicas.

scholarly journals Contribution to the identification of the species Myrcia hatschbachii D. Legrand (Myrtaceae): anatomical and histochemical analyses

2021 ◽  
Vol 43 ◽  
pp. e75
Author(s):  
Larissa Junqueira Gatto ◽  
Vanessa Barbosa Bobek ◽  
Jane Manfron Budel ◽  
Natasha Tiemi Fabri ◽  
Josiane De Fatima Gaspari Dias ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phenolic Compounds ◽  
Vascular Bundle ◽  
Folk Medicine ◽  
Spongy Parenchyma ◽  
Anatomical Features ◽  
Arc Shape ◽  
Histochemical Tests ◽  
Scanning Electron

The genus Myrcia is used in folk medicine to treat diabetes. The plants used in folk medicine require morphological and anatomical references to attest to its authenticity. This is the first report of the microscopic study of Myrcia hatschbachii. In this sense, the aim of the present study was to describe the anatomical characteristics, in order to contribute to the species' identification. For anatomical and surface analyses free-hand sections were prepared and observed using optical microscopy, simultaneously some materials were processed and observed under scanning electron microscopy. In addition, histochemical tests were performed. The anatomical features described here correspond with previously reported features found in other species of the genus Myrcia. Furthermore, the following anatomical markers were observed in Myrcia hatschbachii: druses in the spongy parenchyma, concave-convex midrib, heart-shaped petiole, oval-shaped stem, C-shaped vascular bundle in the midrib and open arc shape with invaginated ends in the petiole; phenolic compounds in the phloem and lignified cells in the pith. The results of anatomical and histochemical analyses provide micromorphological and microchemical features that can help in the taxonomy and proper identification of the species.

Localisation of furanocoumarins in the tissues and on the surface of shoots of Heracleum sosnowskyi

Botany ◽  
2017 ◽  
Vol 95 (11) ◽  
pp. 1057-1070 ◽  
Author(s):  
Elżbieta Weryszko-Chmielewska ◽  
Mirosława Chwil
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Invasive Plant ◽  
Health Hazard ◽  
Leaf Petiole ◽  
Native Flora ◽  
Heracleum Sosnowskyi ◽  
Epidermal Surface ◽  
Light Fluorescence ◽  
Scanning Electron

Heracleum sosnowskyi Manden. was introduced in Poland as a fodder plant. Currently, it is regarded as an invasive plant posing a health hazard to humans and animals and a threat to native flora. The aim of the study was to localise furanocoumarins in the stem and leaf tissues. The investigations were carried out using light, fluorescence, and scanning electron microscopy as well as histochemical assays. The epidermis of the analysed organs bears live, non-capitate hairs with variable length, which contain lipids, essential oils, polysaccharides, tannins, and furanocoumarins. The observations performed with scanning electron microscopy revealed the presence of a foamy substance and furanocoumarin crystals on the surface of the trichomes and other epidermal cells, as well as in the parenchyma cells. Characteristic furanocoumarin autofluorescence was present in the epidermis and on its surface, as well as in the subepidermal parenchyma. Secondary fluorescence was emitted by furanocoumarins in different leaf petiole tissues: psoralen, bergapten, and xanthotoxin. We have detected for the first time the presence of furanocoumarins in different tissues of leaves in H. sosnowskyi. Furanocoumarins were also abundantly present on the epidermal surface of cells. This explains why the contact with the plant is dangerous to humans and results in development of photodermatoses.

scholarly journals A peculiar new species of gall-inducing, clearwing moth (Lepidoptera, Sesiidae) associated with Cayaponia in the Atlantic Forest

ZooKeys ◽  
2019 ◽  
Vol 866 ◽  
pp. 39-63 ◽  
Author(s):  
Gilson R.P. Moreira ◽  
Oleg G. Gorbunov ◽  
Júlia Fochezato ◽  
Gislene L. Gonçalves
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Atlantic Forest ◽  
Average Distance ◽  
Larval Instar ◽  
Plant Parts ◽  
Maximum Likelihood Tree ◽  
Clearwing Moth ◽  
Coi Sequences ◽  
Scanning Electron

Larvae of most clearwing moths (Lepidoptera, Sesiidae) are endophagous borers of many angiosperms, including their fruits, stems, and roots. Their localized feeding may lead to swellings on those plant parts, but whether the structures produced should be considered true galls is still controversial. In this study we describe a peculiar sesiid moth, Neospheciacecidogenasp. nov. whose larvae induce unusual, external galls on Cayponiapilosa (Vell.) Cogn. (Cucurbitaceae) in the Atlantic Forest of southernmost Brazil. The adults, egg, larva, pupa and the gall are described and illustrated based on light and scanning electron microscopy. Galls are cylindrical and unilocular; they are induced individually on axillary buds of the C.pilosa stem. Unlike larvae of other sesiids, those of N.cecidogenasp. nov. lack abdominal pseudopodia, and show reduced stemata and chaetotaxy. Pupation occurs inside the gall, after having overwintered in the last larval instar. A maximum likelihood tree constructed based on mitochondrial DNA (COI) sequences showed that N.cecidogenasp. nov. is monophyletic and has an average distance of 13% to species of Melittia. The genera Neosphecia Le Cerf, 1916 stat. rev., Premelittia Le Cerf, 1916 stat rev., and Melittina Le Cerf, 1917 stat. rev. are restored from synonyms of Melittia Hübner, 1819 [“1816”].

scholarly journals Comparative structure of the osmophores in the flowers of Stanhopea graveolens Lindley and Cycnoches chlorochilon Klotzsch (Orchidaceae)

2012 ◽  
Vol 64 (2) ◽  
pp. 15-24 ◽  
Author(s):  
Mirosława Chwil
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Apical Part ◽  
Ornamental Plant ◽  
Abaxial Surface ◽  
Glandular Hairs ◽  
Anatomical Features ◽  
Cuticular Ornamentation ◽  
Comparative Structure ◽  
Scanning Electron

The genus <i>Tradescantia</i> comprises about 70 species. In Poland <i>Tradescantia x andersoniana</i> is basically grown as an ornamental plant that is recommended for borders and to be planted around garden ponds. The present study investigated flowering as well as the micromorphological and anatomical features of some floral elements of <i>Tradescantia x andersoniana</i> W. Ludw. Rohweder 'Karin'. The macro- and micromorphology of the flowers was examined using stereoscopic, light, and scanning electron microscopy (SEM). Spiderwort produces flowers with a diameter of 4.6-5 cm, which open early in the morning and close at noon. Non-glandular and glandular hairs grow on the abaxial surface of the calyx and on the apical part of the ovary. The glandular hairs develop a several-celled stalk and a unicellular spherical or elongated head. The staminal filaments produce chain-shaped trichomes. Striate cuticular ornamentation is found on their surface and on the epidermis covering the perianth. The striae on the cells of the stamen hairs run to the two poles of the cell. The pistil develops a wet stigma with unfused unicellular papillae. The cuticle on their surface is smooth, whereas on the style near the stigma it forms dense folds.

scholarly journals Morpho-anatomical adaptations of Potamogeton polygonus (Potamogetonaceae) to lotic and lentic environments

Rodriguésia ◽  
2011 ◽  
Vol 62 (4) ◽  
pp. 927-936 ◽  
Author(s):  
Makeli Garibotti Lusa ◽  
Maria Regina Torres Boeger ◽  
Maria Cecília de Chiara Moço ◽  
Cleusa Bona
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phenotypic Plasticity ◽  
Light Microscopy ◽  
Aquatic Macrophytes ◽  
Anatomical Structure ◽  
Southern Brazil ◽  
Anatomical Adaptations ◽  
Stems And Leaves ◽  
Scanning Electron

Abstract Aquatic macrophytes show great phenotypic plasticity and are able to occupy environments with different physicochemical conditions. The present study aimed to characterize morphology and anatomical structure of the pondweed, Potamogeton polygonus Cham. & Schltdl., and to identify adaptive modifications of the plant in lotic and lentic environments. Sampling was carried out in Palmas and General Carneiro, Paraná state, southern Brazil. Ten individuals from each locality were collected. Morpho-anatomical characteristics of the roots, stems and leaves were measured. The anatomical structure was analyzed with light microscopy and scanning electron microscopy. Significant morphological and anatomical adaptive differences were observed between plants of the two environments.

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