scholarly journals The importance of cross-reactivity in grass pollen allergy

2014 ◽  
Vol 66 (3) ◽  
pp. 1149-1155 ◽  
Author(s):  
Ivana Aleksic ◽  
Olga Vuckovic ◽  
Katarina Smiljanic ◽  
Marija Gavrovic-Jankulovic ◽  
Vera Krsmanovic ◽  
...  
Keyword(s):  
Grass Pollen ◽  
Pollen Allergy ◽  
Immunoblot Analysis ◽  
Cross Reactivity ◽  
Phleum Pratense ◽  
Pollen Extract ◽  
Grass Pollen Allergy ◽  
Number Of Patients

According to the data obtained from in vivo and in vitro testing in Serbia, a significant number of patients have allergic symptoms caused by grass pollen. We examined the protein composition of grass pollens (Dactylis glomerata, Lolium perenne and Phleum pratense) and cross-reactivity in patients allergic to grass pollen from our region. The grass pollen allergen extract was characterized by SDS-PAGE, while cross-reactivity of single grass pollens was revealed by immunoblot analysis. A high degree of cross-reactivity was demonstrated for all three single pollens in the sera of allergic patients compared to the grass pollen extract mixture. Confirmation of the existence of cross-reactivity between different antigenic sources facilitates the use of monovalent vaccines, which are easier to standardize and at the same time prevent further sensitization of patients and reduces adverse reactions.

scholarly journals Sensitization to timothy grass pollen allergenic molecules in children

2013 ◽  
Vol 8 ◽  
Author(s):  
Alessandra Scaparrotta ◽  
Marcello Verini ◽  
Nicola Pietro Consilvio ◽  
Anna Cingolani ◽  
Daniele Rapino ◽  
...  
Keyword(s):  
Grass Pollen ◽  
Pollen Allergy ◽  
Phleum Pratense ◽  
Pollen Extract ◽  
Mediterranean Population ◽  
Grass Pollen Allergy ◽  
Timothy Grass ◽  
Timothy Grass Pollen ◽  
Species Specific

Background: Grass pollens are significant elicitors of IgE-mediated allergic disease in the world and timothy (Phleum pratense) is one of the most important pollens of the family. Molecular and biochemical characterization of Phleum pratense has revealed several allergen components: rPhl p 1 and rPhl p 5 have been shown to be “Species Specific Allergens”, while the profilin rPhl p 12 and the calcium-binding protein rPhl p 7 are the principal Cross-Reactive components. Methods: In this study the pattern of sensitization to rPhl p 1, rPhl p 5, rPhl p 7 and rPhl p 12 was analyzed in children with asthma and/or rhinoconjunctivitis and grass pollen allergy, in order to evaluate the frequency of sensitization to allergenic molecules of Phleum pratense among pediatric subjects allergic to grass pollen in a Mediterranean population. The correlation of sensitization to these Phleum allergenic molecules with IgE against grass pollen extract and its variation according to age and level of IgE against grass pollen extract were evaluated. Results: IgE against to rPhl p 1 were found in 99% (205/207) of patients, to rPhl p 5 in 67% (139/207), to rPhl p 12 in 32% (66/207) and to rPhl p 7 only in 5% (10/207). Sensitization only to “Species Specific” (rPhl p1, rPhl p5) allergenic molecules of Phleum pratense was detected in 65% (135/207) of children. Our data show the predominant role of rPhl p 1 in pediatric populations as the most relevant sensitizing allergen detectable at all ages and at all levels of timothy grass pollen-specific IgE antibodies, while the importance of rPhl p 5 rises with the increase of patients’ age and with grass pollen IgE levels. Conclusions: The assessment of sensitization to grass pollen allergenic molecules could help develop a better characterization of allergic sensitization in grass pollen allergy in children, which may be different in every patient. It could also enable clinicians to give more specific and effective immunotherapy, based on allergenic molecule sensitization.

Diagnosis of cypress pollen allergy: in vivo and in vitro standardization of a Juniperus ashei pollen extract

Allergy ◽  
2003 ◽  
Vol 58 (8) ◽  
pp. 808-813 ◽  
Author(s):  
M. Hrabina ◽  
J.-P. Dumur ◽  
H. Sicard ◽  
A. Viatte ◽  
C. Andre
Keyword(s):  
Pollen Allergy ◽  
Pollen Extract ◽  
Juniperus Ashei

Reliability of Basophil Activation Test Using CD203c Expression in Diagnosis of Pollen Allergy

2011 ◽  
Vol 25 (6) ◽  
pp. e225-e231 ◽  
Author(s):  
Seçil Kepil Özdemir ◽  
Deniz Güloğlu ◽  
Betül A. Sin ◽  
Atilla H. Elhan ◽  
Aydan İkincioğulları ◽  
...  
Keyword(s):  
Grass Pollen ◽  
Immunoglobulin E ◽  
Pollen Allergy ◽  
Basophil Activation Test ◽  
Basophil Activation ◽  
Pollen Extract ◽  
Activation Test ◽  
Cutoff Values ◽  
Phl P 5

Background CD203c is a basophil surface marker and its expression is rapidly up-regulated after cross-linking of high-affinity immunoglobulin E (IgE) receptor (FcepsilonR1) by an allergen. CD203c basophil activation tests have been studied for the in vitro diagnosis of several allergic conditions. However, there is limited data about its diagnostic usefulness. The optimum allergen concentrations for stimulation and allergen specific cutoff values remain unknown for a number of allergens. This study was designed to investigate the efficacy of basophil activation test via CD203c in the diagnosis of pollen allergy. Methods The CD203c basophil activation was determined in 31 allergic rhinitis patients with pollen allergy and 9 healthy nonatopic controls during the off-season. CD203c expression was evaluated using three-color staining protocol by flow cytometry. Results After an in vitro stimulation with grass pollen extract, the CD203c assay clearly discriminated pollen-allergic patients from controls (p < 0.001). A dose-dependent increase in the percentages of CD203c-activated basophils was shown in rhinitis patients with pollen allergy (p < 0.001). The sensitivity and specificity was 100% and optimal cutoff values were 14.05 and 10.05% with 45.1 and 4.5 μg/mL Phl p 5 stimulation, respectively. Although the specificity was also 100%, the sensitivity was 93 and 87% and the cutoff values were 5.40 and 5.35% with 4.5 x 10– 4 and 4.5 x 10– 5 micrograms/mL Phl p 5 stimulation, respectively. Conclusion The CD203c basophil activation test seems to be a reliable tool in the diagnosis of grass pollen allergy. It could be used when conventional diagnostic tests fail or can not be performed.

scholarly journals Current strategies of allergen-specific immunotherapy

2015 ◽  
Vol 12 (4) ◽  
pp. 16-26
Author(s):  
K S Pavlova ◽  
O M Kurbacheva
Keyword(s):  
Grass Pollen ◽  
Specific Immunotherapy ◽  
Pollen Allergy ◽  
Pollen Extract ◽  
Efficacy And Safety ◽  
Grass Pollen Allergy ◽  
Allergen Specific Immunotherapy ◽  
Natural Exposure ◽  
Safety Studies ◽  
Exposure Conditions

The article discusses the principles of the optimal allergovaccine choice for allergen-specific immunotherapy (ASIT) for grass-pollen-allergy patients; reviewed efficacy and safety studies of the standardized mixt five-grass-pollen extract. Relatively grass sensitization is preferred use mixt grass-pollen extract is thus more suitable for specific immunotherapy, as it contains a broader repertoire of the epitopes, which approximate to the natural exposure conditions.

Molecular profiles of IgE to Phleum pratense in children with grass pollen allergy: Implications for specific immunotherapy

2012 ◽  
Vol 129 (3) ◽  
pp. 834-839.e8 ◽  
Author(s):  
Salvatore Tripodi ◽  
Tullio Frediani ◽  
Sandra Lucarelli ◽  
Francesco Macrì ◽  
Giuseppe Pingitore ◽  
...  
Keyword(s):  
Grass Pollen ◽  
Specific Immunotherapy ◽  
Pollen Allergy ◽  
Phleum Pratense ◽  
Grass Pollen Allergy ◽  
Molecular Profiles
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