scholarly journals Potential improvement of Lymantria dispar L. management by quercetin

2014 ◽  
Vol 66 (3) ◽  
pp. 1125-1129 ◽  
Author(s):  
Vesna Peric-Mataruga ◽  
Branimir Hackenberger ◽  
Milena Vlahovic ◽  
Larisa Ilijin ◽  
Marija Mrdakovic

Lymantria dispar, a polyphagous insect pest, copes with a wide variety of host-specific allelochemicals. Glutathione S-transferases (GST) are important for catalyzing detoxification in L. dispar. Larval mortality, GST activity in midgut tissue and mass of L. dispar with different trophic adaptations (originating from two forests with a suitable host, Quercus robur, and an unsuitable host, Robinia pseudoacacia), differed after feeding on quercetin supplemented diets (2% or 5% w/w). Quercetin inhibited GST most potently in oak forest larvae that were less adapted to flavonoids in their diet. The larvicidal effect of quercetin on L. dispar larvae depended on the host-use history. We believe this is important in strategies for sustainable control of insect pests.

2016 ◽  
Vol 5 (2) ◽  
pp. 58
Author(s):  
Heri Prabowo ◽  
I.G.A.A. Indrayani

<p>Penggunaan pestisida kimia yang cukup tinggi pada akhir-akhir ini telah menimbulkan dampak negatif ter-hadap lingkungan, sehingga pengendalian hama yang ramah lingkungan sangat diperlukan. Saat ini, peng-gunaan nematoda entomopatogen terutama Steinernema sp., membuka peluang untuk digunakan sebagai pengendalian Achaea janata. Tujuan dari penelitian ini untuk mengetahui keefektifan Steinernema sp. ter-hadap A. Janata , dilaksanakan di laboratorium patologi serangga Balai Penelitan Tanaman Pemanis dan Serat pada bulan Maret sampai dengan Agustus 2011. Penelitian menggunakan rancanagan acak lengkap (RAL) dengan empat ulangan. Setiap ulangan menggunakan 25 larva A. janata instar 2. Larva diekspose de-ngan berbagai variasi konsentrasi Steinernema sp. dan kematian larva diamati setiap hari sampai 120 jam setelah infeksi. Konsentrasi Steinernema sp. yang digunakan adalah 0, 50, 100, 200, 300, dan 400 JI/larva. Hasil penelitian pemberian Steinernema sp. pada konsentrasi 200, 300, dan 400 JI/larva cukup efektif untuk membunuh A. janata dengan persentase berkisar antara 80–94% mulai 48–120 jam setelah perlakuan. Se-makin tinggi konsentrasi nematoda, semakin tinggi mortalitas A. janata. Steinernema sp. dengan konsen-trasi 400 JI/larva paling efektif membunuh larva, menurunkan bobot larva, bobot pupa, jumlah telur yang dihasilkan, dan fertilitas telur.</p><p> </p><p>High intensity of chemical pesticide application has become a serious concern of environmentalists in recent years, because of various negative impacts of it. Therefore, environmentally friendly techniques of controlling insect pest are needed. Recently, the use of entomopathogenic nematodes, especially Steinernema sp., has created new possibilities of promising control techniques against insect pests. The aim of this study was to evaluate the effectiveness of Steinernema sp. to A. janata larvae in laboratory. This research was conducted at the Laboratory of Insect Pathology Laboratory of Indonesian Sweetener and Fiber Crops Research Insti-tute from March to August 2011. Tests used the 2nd stage larvae of Achaea janata, the leaf eater of castor (Ricinus communis). Treatment arranged in a completely randomized design (CRD) with 4 replicates. For each test used 25 larvae which were exposed to various concentrations of Steinernema sp. Concentration of Steinernema sp. used was 0, 50, 100, 200, 300, and 400 infective juvenile/larvae. Daily mortality A. janata larvae, larval and pupal weight, the number of eggs laid, and number of hatch eggs were recorded. Steinernema sp. on concentration of 200; 300; and 400 IJ/larvae was effective to cause mortality of A. janata larvae (80–94% mortality after 48–120 hours). The higher the concentration of the nematode the higher larval mortality. Steinernema sp. with concentration of 400 IJ/ larvae was effective decreasing larval and pupal weight, the number of eggs laid, and fertility of the eggs produced.</p>


2020 ◽  
Vol 31 (1) ◽  
pp. 24-35 ◽  
Author(s):  
Somiahnadar Rajendran

Insects are a common problem in stored produce. The author describes the extent of the problem and approaches to countering it. Stored products of agricultural and animal origin, whether edible or non-edible, are favourite food for insect pests. Durable agricultural produce comprising dry raw and processed commodities and perishables (fresh produce) are vulnerable to insect pests at various stages from production till end-use. Similarly, different animal products and museum objects are infested mainly by dermestids. Insect pests proliferate due to favourable storage conditions, temperature and humidity and availability of food in abundance. In addition to their presence in food commodities, insects occur in storages (warehouses, silos) and processing facilities (flour mills, feed mills). Insect infestation is also a serious issue in processed products and packed commodities. The extent of loss in stored products due to insects varies between countries depending on favourable climatic conditions, and pest control measures adopted. In stored food commodities, insect infestation causes loss in quantity, changes in nutritional quality, altered chemical composition, off-odours, changes in end-use products, dissemination of toxigenic microorganisms and associated health implications. The insects contribute to contaminants such as silk threads, body fragments, hastisetae, excreta and chemical secretions. Insect activity in stored products increases the moisture content favouring the growth of moulds that produce mycotoxins (e.g., aflatoxin in stored peanuts). Hide beetle, Dermestes maculatus infesting silkworm cocoons has been reported to act as a carrier of microsporidian parasite Nosema bombycis that causes pebrine disease in silkworms. In dried fish, insect infestation leads to higher bacterial count and uric acid levels. Insects cause damage in hides and skins affecting their subsequent use for making leather products. The trend in stored product insect pest management is skewing in favour of pest prevention, monitoring, housekeeping and finally control. Hermetic storage system can be supplemented with CO2 or phosphine application to achieve quicker results. Pest detection and monitoring has gained significance as an important tool in insect pest management. Pheromone traps originally intended for detection of infestations have been advanced as a mating disruption device ensuing pest suppression in storage premises and processing facilities; pheromones also have to undergo registration protocols similar to conventional insecticides in some countries. Control measures involve reduced chemical pesticide use and more non-chemical inputs such as heat, cold/freezing and desiccants. Furthermore, there is an expanding organic market where physical and biological agents play a key role. The management options for insect control depend on the necessity or severity of pest incidence. Generally, nonchemical treatments, except heat, require more treatment time or investment in expensive equipment or fail to achieve 100% insect mortality. Despite insect resistance, environmental issues and residue problems, chemical control is inevitable and continues to be the most effective and rapid control method. There are limited options with respect to alternative fumigants and the alternatives have constraints as regards environmental and health concerns, cost, and other logistics. For fumigation of fresh agricultural produce, new formulations of ethyl formate and phosphine are commercially applied replacing methyl bromide. Resistance management is now another component of stored product pest management. In recent times, fumigation techniques have improved taking into consideration possible insect resistance. Insect control deploying nanoparticles, alone or as carriers for other control agents, is an emerging area with promising results. As there is no single compound with all the desired qualities, a necessity has arisen to adopt multiple approaches. Cocktail applications or combination treatments (IGRs plus organophosphorus insecticides, diatomaceous earth plus contact insecticides, nanoparticles plus insecticides/pathogens/phytocompounds and conventional fumigants plus CO2; vacuum plus fumigant) have been proved to be more effective. The future of store product insect pest management is deployment of multiple approaches and/or combination treatments to achieve the goal quickly and effectively.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Abhay Punia ◽  
Nalini Singh Chauhan ◽  
Drishtant Singh ◽  
Anup Kumar Kesavan ◽  
Sanehdeep Kaur ◽  
...  

AbstractThe antibiosis effect of gallic acid on Spodoptera litura F. (Lepidoptera: Noctuidae) and its parasitoid evaluated by feeding six days old larvae on artificial diet incorporated with different concentrations (5 ppm, 25 ppm, 125 ppm, 625 ppm, 3125 ppm) of the phenolic compound revealed higher concentration (LC50) of gallic acid had a negative impact on the survival and physiology of S. litura and its parasitoid Bracon hebetor (Say) (Hymenoptera:Braconidae). The mortality of S. litura larvae was increased whereas adult emergence declined with increasing concentration of gallic acid. The developmental period was delayed significantly and all the nutritional indices were reduced significantly with increase in concentration. Higher concentration (LC50) of gallic acid adversely affected egg hatching, larval mortality, adult emergence and total development period of B. hebetor. At lower concentration (LC30) the effect on B. hebetor adults and larvae was non-significant with respect to control. Gene expression for the enzymes viz., Superoxide dismutase, Glutathione peroxidase, Peroxidase, Esterases and Glutathione S transferases increased while the total hemocyte count of S. litura larvae decreased with treatment. Our findings suggest that gallic acid even at lower concentration (LC30) can impair the growth of S. litura larvae without causing any significant harm to its parasitoid B. hebetor and has immense potential to be used as biopesticides.


Insects ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 23
Author(s):  
Md Munir Mostafiz ◽  
Errol Hassan ◽  
Rajendra Acharya ◽  
Jae-Kyoung Shim ◽  
Kyeong-Yeoll Lee

The Indian meal moth, Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae), is an insect pest that commonly affects stored and postharvest agricultural products. For the control of insect pests and mites, methyl benzoate (MBe) is lethal as a fumigant and also causes contact toxicity; although it has already been established as a food-safe natural product, the fumigation toxicity of MBe has yet to be demonstrated in P. interpunctella. Herein, we evaluated MBe as a potential fumigant for controlling adults of P. interpunctella in two bioassays. Compared to the monoterpenes examined under laboratory conditions, MBe demonstrated high fumigant activity using a 1-L glass bottle at 1 μL/L air within 4 h of exposure. The median lethal concentration (LC50) of MBe was 0.1 μL/L air; the median lethal time (LT50) of MBe at 0.1, 0.3, 0.5, and 1 μL/L air was 3.8, 3.3, 2.8, and 2.0 h, respectively. Compared with commercially available monoterpene compounds used in pest control, MBe showed the highest fumigant toxicity (toxicity order as follows): MBe > citronellal > linalool > 1,8 cineole > limonene. Moreover, in a larger space assay, MBe caused 100% mortality of P. interpunctella at 0.01 μL/cm3 of air after 24 h of exposure. Therefore, MBe can be recommended for use in food security programs as an ecofriendly alternative fumigant. Specifically, it provides another management tool for curtailing the loss of stored food commodities due to P. interpunctella infestation.


2021 ◽  
Vol 3 (2) ◽  
pp. 383-393
Author(s):  
Patient Farsia Djidjonri ◽  
Nukenine Elias Nchiwan ◽  
Hartmut Koehler

The present study investigates the effect of intercropping (maize-cowpea, maize-okra, maize-okra-cowpea, okra-cowpea) compared to insecticide application on the level of infestation of insect pests and the final yield of maize, cowpea and okra. Field experiments were conducted during the 2016 and 2017 cropping seasons in the Guinean Savannah (Dang-Ngaoundere) and Sudano Sahelian (Gouna-Garoua) agro-ecological zones in Cameroon. Our experimental design was a split plot arrangement in a randomized complete block with four replications. The main factor was assigned to the use of insecticide (Cypermethrin) and sub plots were devoted for cropping systems. We compared the efficiency of intercropping to that of Cypermethrin application on the Yield of maize, cowpea and okra as influenced by insect pest damages. The comparison of monocropped sprayed by Cypermethrin to unsprayed showed that, in Dang, insect pests reduced maize yield by 37% and 24% in 2016 and 2017, respectively, whereas in Gouna, it was lower than 8% during the both years. Reduction in seed yield by insect pests on cowpea in Dang represented 47% and 50% in 2016 and 2017, respectively, whereas in Gouna, it was 55% and 63% in 2016 and 2017, respectively. For okra, insect pests reduced okra fruit yield by 25% and 44% in Dang and 23% and 28% in Gouna, respectively, in 2016 and 2017. Crop yield was lower in intercropping compared to monoculture due to competition of plants in association on different resources. Considering the total yields obtained from each intercropping, intercropping trials resulted generally in higher yields compared to mono-culture (LER > 1) in both sites and years but the respective yields were quite different. On the basis of the results obtained, we recommend maize-cowpea intercropping as a sustainable solution to reduce the infestation level of their pest insects.


2017 ◽  
Vol 108 (5) ◽  
pp. 658-666 ◽  
Author(s):  
X. Lin ◽  
Y. Jiang ◽  
L. Zhang ◽  
Y. Cai

AbstractSpodoptera litura is a widespread polyphagous insect pest that can develop resistance and cross-resistance to insecticides, making it difficult to control. Insecticide exposure has previously been linked with induction of specific olfactory-related proteins, including some chemosensory proteins (CSPs) and odorant-binding proteins (OPBs), which may disrupt detection of environmental factors and reduce fitness. However, functional evidence supporting insecticide and OBPs/CSPs mediation remains unknown. Here we fed male S. litura moths with sucrose water containing one of three insecticides, chlorpyrifos, emamectin benzoate or fipronil, and used real-time quantitative polymerase chain reaction and RNAi to investigate OBPs and CSPs expression and their correlations with survival. Chlorpyrifos and emamectin benzoate increased expression of 78% of OBPs, plus 63 and 56% of CSP genes, respectively, indicating a major impact on these gene families. RNAi knockdown of SlituCSP18, followed by feeding with chlorpyrifos or fipronil, decreased survival rates of male moths significantly compared with controls. Survival rate also decreased significantly with the downregulation of SlituOBP9 followed by feeding with chlorpyrifos. Thus, although these three insecticides had different effects on OBP and CSP gene expression, we hypothesize that SlituOBPs and SlituCSPs might mediate their effects by increasing their expression levels to improve survival. Moreover, the differential response of S. litura male moths to the three insecticides indicated the potential specificity of chlorpyrifos affect SlituCSP18 and SlituOBP9 expression.


2021 ◽  
Vol 70 ◽  
pp. 158-166
Author(s):  
Qiang Zhang ◽  
Wei Dou ◽  
Clauvis Nji Tizi Taning ◽  
Guy Smagghe ◽  
Jin-Jun Wang

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Sardul Singh Sandhu ◽  
Anil K. Sharma ◽  
Vikas Beniwal ◽  
Gunjan Goel ◽  
Priya Batra ◽  
...  

The growing demand for reducing chemical inputs in agriculture and increased resistance to insecticides have provided great impetus to the development of alternative forms of insect-pest control. Myco-biocontrol offers an attractive alternative to the use of chemical pesticides. Myco-biocontrol agents are naturally occurring organisms which are perceived as less damaging to the environment. Their mode of action appears little complex which makes it highly unlikely that resistance could be developed to a biopesticide. Past research has shown some promise of the use of fungi as a selective pesticide. The current paper updates us about the recent progress in the field of myco-biocontrol of insect pests and their possible mechanism of action to further enhance our understanding about the biological control of insect pests.


Insects ◽  
2018 ◽  
Vol 9 (4) ◽  
pp. 128 ◽  
Author(s):  
Shovon Chandra Sarkar ◽  
Endong Wang ◽  
Shengyong Wu ◽  
Zhongren Lei

Companion planting is a well-known strategy to manage insect pests and support a natural enemy population through vegetative diversification. Trap cropping is one such type of special companion planting strategy that is traditionally used for insect pest management through vegetative diversification used to attract insect pests away from the main crops during a critical time period by providing them an alternative preferred choice. Trap crops not only attract the insects for feeding and oviposition, but also act as a sink for any pathogen that may be a vector. Considerable research has been conducted on different trap crops as companion plant species to develop improved pest management strategies. Despite this, little consensus exists regarding optimal trap cropping systems for diverse pest management situations. An advantage of trap cropping over an artificially released natural enemy-based biological control could be an attractive remedy for natural enemies in cropping systems. Besides, many trap crop species can conserve natural enemies. This secondary effect of attracting natural enemies may be an advantage compared to the conventional means of pest control. However, this additional consideration requires a more knowledge-intensive background to designing an effective trap cropping system. We have provided information based on different trap crops as companion plant, their functions and an updated list of trap cropping applications to attract insect pests and natural enemies that should be proven as helpful in future trap cropping endeavors.


2011 ◽  
Vol 63 (3) ◽  
pp. 571-577 ◽  
Author(s):  
Vesna Peric-Mataruga ◽  
Marija Mrdakovic ◽  
Milena Vlahovic ◽  
Larisa Ilijin ◽  
Jankovic Tomanic ◽  
...  

The number, morphometric parameters and amount of aminergic neurosecretory product of protocerebral A2 neurosecretory neurons were investigated in the fifth instar of Lymantria dispar caterpillars, following a suitable or unsuitable trophic regime. Caterpillars originated from two populations (Quercus rubra or Robinia pseudoacacia forest) and were differently adapted to trophic stress, i.e. feeding on locust tree leaves - unsuitable host plant. The number of neurosecretory neurons was higher in the caterpillars originated from Robinia population than in Quercus population, regardless of feeding. A2 neurosecretory neurons, nuclei and their nucleoli were larger in caterpillars fed with unsuitable leaves in both populations. There was more aminergic product in the A2 neurosecretory neurons of the caterpillars fed with unsuitable leaves independently of population origin.


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