scholarly journals The effects of estradiol and human chorionic gonadotropin on ACHT cells in peripubertal female rats: A histological and stereological study

2014 ◽  
Vol 66 (1) ◽  
pp. 143-148 ◽  
Author(s):  
Verica Milosevic ◽  
Danijela Todorovic ◽  
Branka Sosic-Jurjevic ◽  
Ivana Medigovic ◽  
Jasmina Pantelic ◽  
...  
Keyword(s):  
Body Weight ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Volume Density ◽  
Female Rats ◽  
Stereological Analysis

The effects of estradiol (EDP) and human chorionic gonadotropin (HCG) on histological and stereological features of adrenocorticotropic (ACTH) cells in peripubertal female rats were examined. The first group of females received five injections of EDP (0.25 mg/kg b.w.), every second day from the 4th to 14th day after birth, and was killed at the peripubertal stage. The second group of females was given two injections of pregnyl-gonadotrophinum chorionicum (HCG; 50 IU/kg body weight) on the 36th and 37th days after birth, and it was killed 24 h after the last treatment. The controls were injected with an equivalent volume of the corresponding vehicle. ACTH cells were immunohistochemically labeled and stereologically evaluated. Stereological analysis showed that the volume of ACTH cells and their volume density in peripubertal females treated with EDP, were decreased by 15.6% and 53.8% (p<0.05), respectively, compared to the controls. In HCG-treated animals, the observed parameters were increased by 39.2% and 15.4% (p<0.05), respectively, in comparison with the control females. These findings suggest that the application of EDP or HCG exerted opposite effects on the stereological features of pituitary ACTH cells.

scholarly journals Immunohistomorphometric Features of ACTH Cells in Juvenile Rats after Treatment with Estradiol or Human Chorionic Gonadotropin

2012 ◽  
Vol 31 (1) ◽  
pp. 34-39 ◽  
Author(s):  
Verica Milošević ◽  
Danijela Todorović ◽  
Miroslava Veličković ◽  
Nataša Ristić ◽  
Gordana Ušćebrka ◽  
...  
Keyword(s):  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Neonatal Period ◽  
Secretory Activity ◽  
Treated Group ◽  
Inhibitory Effect ◽  
Volume Density ◽  
Female Rats ◽  
Female Rat ◽  
Juvenile Rats

Immunohistomorphometric Features of ACTH Cells in Juvenile Rats after Treatment with Estradiol or Human Chorionic GonadotropinEstradiol and human chorionic gonadotropin (hCG) are very important in controlling the secretory activity of hormone producing cells in the female rat pituitary glands. The aim of the present study was to examine the morphometric parameters of immunohistochemically labeled ACTH cells in juvenile (16th day) female rat pituitaries after treatment with five doses of estradiol dipropionate (EDP) and two doses of hCG during the neonatal period of life. The controls were treated on the same schedule with an equivalent volume of vehicle. All animals were sacrificed 24 h after the last treatment. ACTH-producing cells were studied using the peroxidase-antiperoxidase immunohistochemical procedure. The absolute and relative pituitary weights were increased (p<0.05) only in the EDP treated group by 120.0% and by 121.1% respectively, in comparison with the controls. In this group, the volume of ACTH cells, volume of their nuclei and volume density were significantly decreased (p<0.05) by 6.4%, 33.3% and 46.2% respectively, compared to the corresponding controls. After treatment with hCG, there were no significant (p>0.05) changes neither in the volume of ACTH cells nor in the volume of their nuclei, in comparison with the controls. On the basis of the results obtained in our study, it can be concluded that EDP, injected into female rats during the neonatal period of life, has an inhibitory effect on the immunohistomorphometric parameters of ACTH cells, but such an effect is not clearly expressed after treatment with hCG.

scholarly journals Immunohistomorphometric Characteristics Of Pituitary GH Cells In Infant And Peripubertal Female Rats After Treatment With Estradiol Or Human Chorionic Gonadotropin

2015 ◽  
Vol 16 (4) ◽  
pp. 281-285
Author(s):  
Natasa Ristic ◽  
Vladimir Ajdzanovic ◽  
Svetlana Trifunovic ◽  
Nasta Tanic ◽  
Nada Bujisic ◽  
...  
Keyword(s):  
Cell Volume ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Neonatal Period ◽  
Volume Density ◽  
Morphometric Parameters ◽  
Female Rats ◽  
Inhibitory Effects ◽  
Control Groups ◽  
Gh Cells

AbstractThe effects of estradiol-dipropionate (EDP) or human chorionic gonadotropin (hCG) on immunohistomorphometric characteristics of pituitary GH cells in infant and peripubertal female rats were investigated. The first group of females received five injections of EDP (0.25 mg/kg b.w.) during the neonatal period of life, and was further divided into two subgroups which were sacrificed at the infantile period (17th day) or at the peripubertal period (38th day). The second group received two doses of hCG (50 IU/kg b.w.) on the 15th and 16th day of life in the first subgroup, and on the 36th and 37th days of life in the second subgroup, while they were sacrificed 24 h after the last treatment, respectively. The control females were injected with an equivalent volume of the vehicle and sacrificed according to the appropriate schedules as the hormone treated rats. EDP treatment decreased GH cell volume density in infant and peripubertal females, by 38% and 76% (p<0.05) respectively, in comparison with the controls. The number of GH cells per mm2 in infantile and peripubertal period was decreased in EDP treated animals by 26% and 53% (p<0.05) respectively, compared to the controls. Also, upon EDP treatment in both periods, GH cells were diminished in size and less intensely immunolabelled than in the control groups. The morphometric parameters in animals treated with hCG were insignificantly changed in both analyzed periods, in comparison with the controls. Unlike hCG, EDP manifested clear inhibitory effects on the immunohistomorphometric characteristics of GH cells in examined female rats.

Administration of human chorionic gonadotropin affects sleep-wake phases and other associated behaviors in cycling female rats

1994 ◽  
Vol 654 (2) ◽  
pp. 181-190 ◽  
Author(s):  
P. Toth ◽  
H. Lukacs ◽  
E.S. Hiatt ◽  
K.H. Reid ◽  
V. Iyer ◽  
...  
Keyword(s):  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Female Rats

scholarly journals Induced maturation of eel Anguilla bicolor using different hormone combination

2015 ◽  
Vol 13 (2) ◽  
pp. 189
Author(s):  
Agus Oman Sudrajat ◽  
Antharest Sugati ◽  
, Alimuddin
Keyword(s):  
Growth Hormone ◽  
Body Weight ◽  
Body Length ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Gonadal Development ◽  
The Body ◽  
Gonad Maturation ◽  
Pregnant Mare Serum Gonadotropin ◽  
Serum Gonadotropin

<p class="BasicParagraph" align="center"><strong>ABSTRACT</strong></p><p class="BasicParagraph" align="center"><strong> </strong></p><p class="Pa2">Artificial reproduction of eel <em>Anguilla bicolor </em>is not yet well-established because of insufficient broodstock number. In this research, induction of Indonesian eel gonad maturation was performed by hormonal with a combination of <em>pregnant mare serum gonadotropin </em>(PMSG), <em>human chorionic gonadotropin </em>(HCG) antidopamin and recombinant <em>growth hormone </em>(rGH). This research consisted of five treatments namely: control (NaCl 0,9%), PMSG 20 IU/ kg, PMSG 20 IU/kg + antidopamin 10 ppm/kg, PMSG 20 IU/kg + antidopamin 10 ppm/kg + rGH 10 μg/kg dan PMSG 20 IU/kg + HCG 10 IU/kg. Each treatment contained 10 fishes. Hormonal induction was conducted by intramuscular injections, as much as five times at intervals of seven days. Furthermore observations on gonadal development were performed after injection for 21 days. The results showed that the treatment generated pregnancy level of 100%, while control was 0%. The best treatment was PMSG 20 IU/kg + antidopamin 10 ppm/kg+ rGH 10 μg/kg, seen from a more mature phase of the gametes, spermatocytes in male and oocytes with perinukleolar phase in female fish. Eel at the body weight of 120.4 to 207.8 g and at the body length of 40.9 to 43.1 cm was male, at the body weight of 274.8 g and at the body length of 47 cm was in intersexual phase, and at the body weight of 323.4 g and at the body length of 53 cm was female.</p><p class="Default"> </p><p class="Pa2">Keywords: <em>Anguilla bicolor</em>, antidopamin, hormones, PMSG, rGH, HCG</p><br /><p class="BasicParagraph"> </p><p class="BasicParagraph"> </p><p class="BasicParagraph" align="center"><strong>ABSTRAK</strong></p><p class="BasicParagraph"><strong> </strong></p><p class="Pa2">Pemijahan ikan sidat secara buatan belum dapat dilakukan karena keterbatasan induk matang gonad. Penelitian ini dilakukan untuk mengetahui pengaruh pemberian hormon terhadap percepatan proses perkembangan gonad ikan sidat (<em>Anguilla bicolor</em>). Hormon yang digunakan adalah kombinasi dari <em>pregnant mare serum gonadotropin </em>(PMSG), <em>human chorionic gonadotropin </em>(HCG), antidopamin dan recombinant <em>growth hormone </em>(rGH). Induksi hormonal untuk mempercepat perkembangan gonad ikan sidat dilakukan melalui lima perlakuan yaitu yaitu kontrol (NaCl 0,9%), PMSG 20 IU/kg, PMSG 20 IU/kg+antidopamin 100 ppm/kg, PMSG 20 IU/kg+antidopamin 100 ppm/ kg+rGH 10 μg/kg dan PMSG 20 IU/kg+HCG 10 IU/kg. Setiap perlakuan dilakukan pada sepuluh ekor ikan sidat. Aplikasi induksi hormonal dilakukan melalui penyuntikan secara intramuskular sebanyak lima kali dengan interval tujuh hari sekali, selanjutnya dilakukan pengamatan terhadap perkembangan gonad selama 21 hari dengan interval tujuh hari sekali setelah penyuntikan. Hasil penelitian menunjukkan bahwa perlakuan hormonal menyebabkan tingkat kebuntingan sebanyak 100% pada ikan perlakuan, sedangkan kontrol sebanyak 0%. Kombinasi terbaik adalah PMSG+antidopamin+rGH, terlihat dari fase gamet yang lebih matang yaitu mencapai fase spermatosit pada ikan jantan dan oosit dengan fase perinukleolar pada ikan betina. Berdasarkan hasil penelitian, ikan sidat dengan bobot 120,4−207,8 g dan panjang 40,9−43,1 cm masih berjenis kelamin jantan. Ikan dengan bobot 274,8 g dan panjang 47 cm masih berada pada fase peralihan kelamin, sedangkan pada bobot 323,4 dan panjang 53 cm sudah berjenis kelamin betina.</p><p class="Default"> </p><p class="Pa2">Kata kunci: <em>Anguilla bicolor</em>, antidopamin, hormon, PMSG, rGH, HCG</p>

scholarly journals Effect of exogenous human chorionic gonadotropin on ovulation in mice

2020 ◽  
Vol 23 (8) ◽  
pp. 1006-1010
Author(s):  
S. Ya. Amstislavsky ◽  
S. V. Ranneva ◽  
D. S. Ragaeva ◽  
E. A. Chuyko ◽  
A. M. Popkova ◽  
...  
Keyword(s):  
Body Weight ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Assisted Reproductive Technologies ◽  
Polar Body ◽  
Reproductive Technologies ◽  
The Body ◽  
Hormonal Stimulation ◽  
Experimental Group

The implementation of assisted reproductive technologies (ART), hormonal stimulation in particular, may change the quality of ovulated oocytes. The purpose of our work was to study ovulation in CD1 mice after their stimulation with human chorionic gonadotropin (hCG) and to investigate the effects of such hormonal stimulation on the pregnancy duration, fetal losses and the weight of the offspring. No significant differences were found in the total number of ovulated oocytes or in the number of immature (without a polar body) ovulated oocytes; nor were there differences between the groups in the number of oocytes with a developing polar body. However, the number of matured oocytes with a distinct polar body was significantly higher (p < 0.05) in mice stimulated with hCG (experimental group) as compared with the controls (6.2 ± 0.86 and 2.2 ± 0.97, respectively). No significant differences were observed between the experimental and control mice in the duration of pregnancy or in the numbers of term offspring, including the percentage of live and stillborn pups. However, the body weight of the offspring in the experimental group was significantly lower (p < 0.001) as compared with the controls on the fifth day after birth (3.16 ± 0.09 and 3.76 ± 0.07, respectively). Thus, exogenous hCG facilitates the development of mouse oocytes in vivo, which leads to the larger number of their mature forms at ovulation, however, the offspring born after hCG-stimulated pregnancy was characterized by a lower body weight on the fifth day after birth.

scholarly journals INDUKSI OVULASI DAN PEMIJAHAN IKAN AGAMYSIS (Agamyxis albomaculatus) MENGGUNAKAN HORMON YANG BERBEDA

2017 ◽  
Vol 12 (2) ◽  
pp. 169
Author(s):  
Bastiar Nur ◽  
Asep Permana ◽  
Agus Priyadi ◽  
Siti Zuhriyah Mustofa ◽  
Siti Murniasih
Keyword(s):  
Body Weight ◽  
Latent Period ◽  
Aromatase Inhibitor ◽  
Freshwater Fish ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Fertilization Rate ◽  
Hatching Rate ◽  
Recirculation System ◽  
Ad Libitum

Ikan agamysis (Agamyxis albomaculatus) merupakan ikan hias air tawar introduksi yang memiliki potensi cukup bagus untuk dikembangkan di Indonesia, namun masih bermasalah dalam budidayanya. Penelitian ini bertujuan untuk menguji efektivitas kombinasi hormon berbeda dalam menginduksi ovulasi dan pemijahan ikan agamysis. Jumlah ikan yang digunakan sebanyak 30 ekor (15 jantan dan 15 betina) dengan ukuran panjang 10-15 cm dan bobot 50-200 g. Ikan uji dipelihara dalam akuarium sistem resirkulasi dan diberi pakan cacing tanah dua kali sehari secara ad libitum. Perlakuan yang diberikan berupa penggunaan beberapa jenis hormon yaitu: (A) LHRHa dan antidopamin (ovaprim) dosis 0,7 mL/kg; (B) human chorionic gonadotropin (hCG) dosis 500 IU/kg; (C) aromatase inhibitor (AI) dosis 10 mg/kg; (D) kombinasi AI dosis 10 mg/kg + ovaprim dosis 0,7 mL/kg; serta (E) kombinasi hCG dosis 500 IU/kg + ovaprim dosis 0,7 mg/kg. Hormon diberikan melalui penyuntikan secara intramuskuler. Hasil penelitian menunjukkan bahwa penggunaan kombinasi hormon hCG dosis 500 IU/kg + ovaprim dosis 0,7 mL/kg; memberikan respons pemijahan terbaik dengan waktu laten 7-12 jam; bobot telur ovulasi 7,3–9,4 g; derajat pembuahan 5,71%-34,7%; dan derajat penetasan 32,5%-50,44%. Penggunaan kombinasi hormon hCG dan ovaprim efektif menstimulasi proses ovulasi telur sehingga dapat menjadi acuan untuk peningkatan produksi larva ikan agamysis dalam pemijahan buatan.Agamysis (Agamyxis albomaculatus) is an introduced freshwater fish that has the potential to be developed in Indonesia. Yet, the fish has not been successfully cultivated. This research was conducted to determine the effectiveness of different hormone combinations to induce ovulation and spawning of agamysis. This research used 30 fishes (15 male and 15 female) ranged 10-15 cm in length and 50-200 g in body weight. The fish were stocked in aquaria equipped with recirculation system and fed with earthworm by ad libitum twice a day. The treatments were: (A) LHRHa and antidopamin (ovaprim) dose of 0.7 mL/ kg, (B) human chorionic gonadotropin (hCG) dose of 500 IU/kg, (C) aromatase inhibitor (AI) dose of 10 mg/kg, (D) a combination of AI dose of 10 mg/kg + ovaprim dose of 0.7 mL/kg, and (E) a combination of hCG dose of 500 IU/kg + ovaprim dose of 0.7 mg/kg. The hormones were given through intramuscularly injection. The results showed that the combination of ovaprim dose of 0.7 mL/kg and hCG dose of 500 IU/kg produced the best spawning response, with the latent period of 7-12 hours, weight of ovulated eggs of 7.3-9.4 g, fertilization rate of 5.71% to 34.7% and hatching rate between 32.5% - 50.44%. In conclusion, the combination of hCG and ovaprim was found to be more effective to stimulate egg ovulation of agamysis and could be used in improving larvae production.

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