scholarly journals Effect of exogenous human chorionic gonadotropin on ovulation in mice

2020 ◽  
Vol 23 (8) ◽  
pp. 1006-1010
Author(s):  
S. Ya. Amstislavsky ◽  
S. V. Ranneva ◽  
D. S. Ragaeva ◽  
E. A. Chuyko ◽  
A. M. Popkova ◽  
...  
Keyword(s):  
Body Weight ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Assisted Reproductive Technologies ◽  
Polar Body ◽  
Reproductive Technologies ◽  
The Body ◽  
Hormonal Stimulation ◽  
Experimental Group

The implementation of assisted reproductive technologies (ART), hormonal stimulation in particular, may change the quality of ovulated oocytes. The purpose of our work was to study ovulation in CD1 mice after their stimulation with human chorionic gonadotropin (hCG) and to investigate the effects of such hormonal stimulation on the pregnancy duration, fetal losses and the weight of the offspring. No significant differences were found in the total number of ovulated oocytes or in the number of immature (without a polar body) ovulated oocytes; nor were there differences between the groups in the number of oocytes with a developing polar body. However, the number of matured oocytes with a distinct polar body was significantly higher (p < 0.05) in mice stimulated with hCG (experimental group) as compared with the controls (6.2 ± 0.86 and 2.2 ± 0.97, respectively). No significant differences were observed between the experimental and control mice in the duration of pregnancy or in the numbers of term offspring, including the percentage of live and stillborn pups. However, the body weight of the offspring in the experimental group was significantly lower (p < 0.001) as compared with the controls on the fifth day after birth (3.16 ± 0.09 and 3.76 ± 0.07, respectively). Thus, exogenous hCG facilitates the development of mouse oocytes in vivo, which leads to the larger number of their mature forms at ovulation, however, the offspring born after hCG-stimulated pregnancy was characterized by a lower body weight on the fifth day after birth.

scholarly journals Induced maturation of eel Anguilla bicolor using different hormone combination

2015 ◽  
Vol 13 (2) ◽  
pp. 189
Author(s):  
Agus Oman Sudrajat ◽  
Antharest Sugati ◽  
, Alimuddin
Keyword(s):  
Growth Hormone ◽  
Body Weight ◽  
Body Length ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Gonadal Development ◽  
The Body ◽  
Gonad Maturation ◽  
Pregnant Mare Serum Gonadotropin ◽  
Serum Gonadotropin

<p class="BasicParagraph" align="center"><strong>ABSTRACT</strong></p><p class="BasicParagraph" align="center"><strong> </strong></p><p class="Pa2">Artificial reproduction of eel <em>Anguilla bicolor </em>is not yet well-established because of insufficient broodstock number. In this research, induction of Indonesian eel gonad maturation was performed by hormonal with a combination of <em>pregnant mare serum gonadotropin </em>(PMSG), <em>human chorionic gonadotropin </em>(HCG) antidopamin and recombinant <em>growth hormone </em>(rGH). This research consisted of five treatments namely: control (NaCl 0,9%), PMSG 20 IU/ kg, PMSG 20 IU/kg + antidopamin 10 ppm/kg, PMSG 20 IU/kg + antidopamin 10 ppm/kg + rGH 10 μg/kg dan PMSG 20 IU/kg + HCG 10 IU/kg. Each treatment contained 10 fishes. Hormonal induction was conducted by intramuscular injections, as much as five times at intervals of seven days. Furthermore observations on gonadal development were performed after injection for 21 days. The results showed that the treatment generated pregnancy level of 100%, while control was 0%. The best treatment was PMSG 20 IU/kg + antidopamin 10 ppm/kg+ rGH 10 μg/kg, seen from a more mature phase of the gametes, spermatocytes in male and oocytes with perinukleolar phase in female fish. Eel at the body weight of 120.4 to 207.8 g and at the body length of 40.9 to 43.1 cm was male, at the body weight of 274.8 g and at the body length of 47 cm was in intersexual phase, and at the body weight of 323.4 g and at the body length of 53 cm was female.</p><p class="Default"> </p><p class="Pa2">Keywords: <em>Anguilla bicolor</em>, antidopamin, hormones, PMSG, rGH, HCG</p><br /><p class="BasicParagraph"> </p><p class="BasicParagraph"> </p><p class="BasicParagraph" align="center"><strong>ABSTRAK</strong></p><p class="BasicParagraph"><strong> </strong></p><p class="Pa2">Pemijahan ikan sidat secara buatan belum dapat dilakukan karena keterbatasan induk matang gonad. Penelitian ini dilakukan untuk mengetahui pengaruh pemberian hormon terhadap percepatan proses perkembangan gonad ikan sidat (<em>Anguilla bicolor</em>). Hormon yang digunakan adalah kombinasi dari <em>pregnant mare serum gonadotropin </em>(PMSG), <em>human chorionic gonadotropin </em>(HCG), antidopamin dan recombinant <em>growth hormone </em>(rGH). Induksi hormonal untuk mempercepat perkembangan gonad ikan sidat dilakukan melalui lima perlakuan yaitu yaitu kontrol (NaCl 0,9%), PMSG 20 IU/kg, PMSG 20 IU/kg+antidopamin 100 ppm/kg, PMSG 20 IU/kg+antidopamin 100 ppm/ kg+rGH 10 μg/kg dan PMSG 20 IU/kg+HCG 10 IU/kg. Setiap perlakuan dilakukan pada sepuluh ekor ikan sidat. Aplikasi induksi hormonal dilakukan melalui penyuntikan secara intramuskular sebanyak lima kali dengan interval tujuh hari sekali, selanjutnya dilakukan pengamatan terhadap perkembangan gonad selama 21 hari dengan interval tujuh hari sekali setelah penyuntikan. Hasil penelitian menunjukkan bahwa perlakuan hormonal menyebabkan tingkat kebuntingan sebanyak 100% pada ikan perlakuan, sedangkan kontrol sebanyak 0%. Kombinasi terbaik adalah PMSG+antidopamin+rGH, terlihat dari fase gamet yang lebih matang yaitu mencapai fase spermatosit pada ikan jantan dan oosit dengan fase perinukleolar pada ikan betina. Berdasarkan hasil penelitian, ikan sidat dengan bobot 120,4−207,8 g dan panjang 40,9−43,1 cm masih berjenis kelamin jantan. Ikan dengan bobot 274,8 g dan panjang 47 cm masih berada pada fase peralihan kelamin, sedangkan pada bobot 323,4 dan panjang 53 cm sudah berjenis kelamin betina.</p><p class="Default"> </p><p class="Pa2">Kata kunci: <em>Anguilla bicolor</em>, antidopamin, hormon, PMSG, rGH, HCG</p>

scholarly journals Histological Feature of Ovarian Structures Throughout the Reproductive Cycle in Alpine Goats (Capra Hircus)

2019 ◽  
Vol 42 (1) ◽  
pp. 23-34
Author(s):  
Debora Groppetti ◽  
Alessandro Pecile ◽  
Stefano Frattini ◽  
Giulio Pagnacco ◽  
Silvana Arrighi
Keyword(s):  
Body Weight ◽  
Reproductive Cycle ◽  
Ovarian Function ◽  
Assisted Reproductive Technologies ◽  
Small Ruminants ◽  
Reproductive Technologies ◽  
The Body ◽  
Capra Hircus ◽  
Corpora Lutea ◽  
Domestic Species

AbstractThe knowledge about ovarian physiology in small ruminants is still limited, especially when compared to other domestic species. Ovarian function in goats is mainly assessed by ultrasonographic techniques, whereas a quali-quantitative feature of the follicular and luteal structures throughout the reproductive cycle in naturally cycling goats is scarce. This study provides a detailed description of the functional morphology and size of 742 ovarian structures (follicles, corpora hemorrhagica and corpora lutea) in relation to the oestrus phase, the body weight and the age of 25 Alpine goats (Capra hircus). The current study demonstrated that, the number and size of the follicles were related to the stage of the reproductive cycle (P < 0.0001). Also, the mean number of follicles was high during both prepubertal anestrus and diestrus, whereas it was low in the oestrus. Large (3-4 mm in diameter) and very large follicles (> 4 mm) (P < 0.001), and small follicles (< 2 mm; P < 0.05) varied throughout the reproductive cycle, while medium follicles (2-3 mm) were invariably observed. Large and very large follicles were predominant during the diestrus phase and small follicles in the prepubertal anestrus. It is evident from the current study that the number of follicles (P < 0.05) was significantly affected with both body weight and age. On the other hand, the size of follicles was significantly affected with body weight only (P < 0.0001). These results could contribute to a deeper understanding of ovarian transformations with important implications in assisted reproductive technologies, thereby concurring in advancing the efficiency of ultrasound in breeding programs for this species.

scholarly journals Relapse of Human Chorionic Gonadotropin-Induced Hyperthyroidism and Severe Hyperemesis Gravidarum Secondary to Twin-Twin Transfusion Syndrome, With Rapid Recovery Following Fetoscopic Laser Coagulation: Case Report

2021 ◽  
Vol 12 ◽  
Author(s):  
Faiza Lamine ◽  
Chiara Camponovo ◽  
David Baud ◽  
Dominique Werner ◽  
Laura Marino ◽  
...  
Keyword(s):  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Twin Pregnancy ◽  
Assisted Reproductive Technologies ◽  
Hyperemesis Gravidarum ◽  
Reproductive Technologies ◽  
Laser Coagulation ◽  
Monochorionic Twin ◽  
Fetoscopic Laser Coagulation ◽  
Twin Pregnancies

BackgroundLimited data have shown that, compared to uncomplicated twin pregnancies, pregnancies complicated by twin-twin transfusion syndrome (TTTS), a life-threatening condition, are associated with higher maternal serum levels of both human chorionic gonadotropin (hCG) and thyroid hormones. With the continuing expansion of assisted reproductive technologies, the rate of twin pregnancies, including those complicated by TTTS and associated hyperemesis gravidarum, is expected to increase further. Therefore, detailed descriptions of the maternal and fetal clinical outcomes of maternal thyrotoxicosis linked to TTTS can be useful for timely diagnosis and management. However, such descriptions are currently lacking in the literature.Case PresentationWe report the case of a 30-year-old woman carrying a monochorionic twin pregnancy complicated by TTTS that induced a relapse of severe hyperemesis gravidarum with overt non-autoimmune hyperthyroidism at 17 weeks of gestation. Following fetoscopic laser coagulation (FLC), both hyperemesis and hyperthyroidism improved within 1 week.ConclusionsThe present experience contributes to the knowledge base on maternal thyrotoxicosis linked to TTTS and can be useful in the diagnosis and treatment of future cases; it also emphasizes the need for a high degree of clinical suspicion and for close collaboration between endocrinologists and obstetricians. Another key point is that TTTS-associated hyperemesis gravidarum and maternal hyperthyroidism should be considered in the differential diagnosis of refractory or relapsing hyperemesis gravidarum in women with monochorionic twin pregnancy, because this condition may require more stringent supportive treatment before and during the FLC procedure when the mother is overtly hyperthyroid.

321 CYTOSKELETAL ALTERATIONS OF EQUINE OOCYTES THAT FAILED TO CLEAVE AFTER INTRACYTOPLASMIC SPERM INJECTION: EVALUATION OF MATERNAL AND CELL AGING

2015 ◽  
Vol 27 (1) ◽  
pp. 249
Author(s):  
E. Ruggeri ◽  
K. DeLuca ◽  
C. Galli ◽  
G. Lazzari ◽  
J. DeLuca ◽  
...  
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Assisted Reproductive Technologies ◽  
Polar Body ◽  
Reproductive Technologies ◽  
Oocyte Quality ◽  
Cell Aging ◽  
Zygote Development ◽  
Β Tubulin

Intracytoplasmic sperm injection (ICSI) is used for assisted fertilization of equine oocytes. However, not all oocytes cleave after ICSI. Maternal aging deleteriously affects fertility in mares and women, with reduced oocyte quality and success of assisted reproductive technologies. In the oocyte, senescence and cell-programmed death begins after maturation; the extent that maternal age affects these events is unknown. We hypothesised that formation of α/β tubulin asters and f-actin bubbles are associated with aging of the oocyte in vitro and/or aging of the oocyte in vivo, in aged donors. In Exp 1, oocytes were collected from ovaries obtained from an abattoir and matured for 28 h and selected for polar body extrusion (0 h). At 0, 24, and 48 h, oocytes (n = 38 total) were fixed in MTSB-XF and transferred into wash solution with 1% BSA and 0.1% Triton X-100 in PBS for immunostaining. For experiment 2, oocytes were collected from preovulatory follicles of mares (9–25 yr) in a clinical ICSI program and injected with sperm from various stallions after extrusion of a polar body. Between 24 to 51 h after ICSI, uncleaved oocytes (n = 52, single cell without evidence of fragmentation or indentation of the oolemma) were fixed. All oocytes were incubated with α/β tubulin and human-anti-centromere antibody-CREST/ACA (1 : 100 each). Following primary incubation, oocytes were washed and incubated with Alexa 488, Alexa 647, Alexa 561-phalloidin, and Hoechst 33258. Images and Z-stacks were acquired on an Olympus IX81 spinning disk confocal microscope. Morphometric and intensity analyses of images were performed using SlideBook software (Denver, CO). Student's t-test, Fisher's exact test, and chi-square analyses were used for statistical comparisons. After aging in vitro (experiment 1), the number of oocytes with tubulin multiasters increased (P < 0.001; 9% at 0 h, 14% at 24 h, 85% at 48 h); however, actin bubbling was observed in only 5/38 (13%) oocytes, with no effect of incubation time. In experiment 2, tubulin multiasters were present in 62% of oocytes that failed to cleave. More multiasters were observed per oocyte from mares ≤13 yr than ≥20 yr (P = 0.03) and fixed at 24 to 28 h than 44 to 51 h (P = 0.04). Actin bubbles were observed in 71% of oocytes that failed to cleave after ICSI, with more actin bubbles in oocytes from mares ≥20 yr than ≤13 yr (P = 0.01) and fixed 44 to 51 h versus 24 to 28 h after ICSI (P = 0.05). The sum intensity and area of the actin bubbles were higher in oocytes fixed at 44 to 51 h than 24 to 28 h (P = 0.01 and P = 0.04). The area occupied by the actin bubbles was larger (P = 0.05) in oocytes from mares ≥20 yr than ≤13 yr. This study demonstrates actin bubbles and tubulin asters are involved in oocyte aging and cytoskeleton remodelling with or without fertilization. Although actin structures were associated with donor age and hours after ICSI, they were not present in unfertilized oocytes aged in vitro. Multiaster formation was associated with cell senescence in oocytes aged in vitro. Although not previously reported for the equine oocyte, multiaster formation appeared to be an initial fertilization event within the oocyte associated with attempted zygote development.

Re-imagining Reproduction: Unsettling Metaphors in the History of Imperial Science and Commercial Surrogacy in India

Somatechnics ◽  
2015 ◽  
Vol 5 (1) ◽  
pp. 88-103 ◽  
Author(s):  
Kalindi Vora
Keyword(s):  
Assisted Reproductive Technologies ◽  
Reproductive Technologies ◽  
The Body ◽  
Body Parts ◽  
Colonial History ◽  
Women's Bodies ◽  
Commercial Surrogacy ◽  
Art Practices ◽  
History Of ◽  
Travel Industry

This paper provides an analysis of how cultural notions of the body and kinship conveyed through Western medical technologies and practices in Assisted Reproductive Technologies (ART) bring together India's colonial history and its economic development through outsourcing, globalisation and instrumentalised notions of the reproductive body in transnational commercial surrogacy. Essential to this industry is the concept of the disembodied uterus that has arisen in scientific and medical practice, which allows for the logic of the ‘gestational carrier’ as a functional role in ART practices, and therefore in transnational medical fertility travel to India. Highlighting the instrumentalisation of the uterus as an alienable component of a body and subject – and therefore of women's bodies in surrogacy – helps elucidate some of the material and political stakes that accompany the growth of the fertility travel industry in India, where histories of privilege and difference converge. I conclude that the metaphors we use to structure our understanding of bodies and body parts impact how we imagine appropriate roles for people and their bodies in ways that are still deeply entangled with imperial histories of science, and these histories shape the contemporary disparities found in access to medical and legal protections among participants in transnational surrogacy arrangements.

scholarly journals Clinical and in Vivo Response Following Surgery or Surgery Plus Adjuvant Chemotherapy or Immunotherapy for Colorectal Carcinoma in a Rat Model

1983 ◽  
Vol 76 (10) ◽  
pp. 833-840 ◽  
Author(s):  
A K House ◽  
M A L Maley
Keyword(s):  
Body Weight ◽  
Adjuvant Chemotherapy ◽  
Surgical Excision ◽  
The Body ◽  
Recurrent Tumour ◽  
Mesenteric Node ◽  
And Control ◽  
Tumour Weight

Two cohorts of rats, 240 with colon cancer and 150 controls, were assessed clinically and immunologically for their response to tumour and its management which was either by surgical excision alone or by surgical excision combined with either adjuvant chemotherapy or immunotherapy. The histology and invasion characteristics were observed for similarity with those of human lesions. Metastases were found in liver, lymph nodes, the peritoneum or lungs in 27% of animals during follow up. Significantly fewer adjuvant-treated rats had metastases than those receiving surgery alone ( P < 0.05), and less total tumour weight was found in the adjuvant-treated rats at four ( P < 0.03) and six ( P < 0.001) weeks postoperatively. Animals in the adjuvant immunotherapy group survived longer than in either other group ( P < 0.001). The crude parameters of host response to tumour, body, spleen and mesenteric lymph node weight were recorded and the latter two indexed to body weight. The body weight of tumour and control rats increased significantly with time ( P < 0.04). The spleen and mesenteric node indices were significantly ( P < 0.04) greater in tumour than control rats and were varied by recurrent tumour growth and by the adjuvant treatment administered postoperatively.

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