scholarly journals Antibacterial and free-radical-scavenging properties of Stachys schtschegleevii (Lamiaceae)

2010 ◽  
Vol 62 (4) ◽  
pp. 941-945 ◽  
Author(s):  
Malvika Abichandani ◽  
Lutfun Nahar ◽  
Poonam Singh ◽  
Rohit Chitnis ◽  
H. Nazemiyeh ◽  
...  
Keyword(s):  
Free Radical ◽  
Bacterial Infections ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Positive Control ◽  
Free Radical Scavenging ◽  
Meoh Extract ◽  
Inflammatory Conditions ◽  
Iranian Traditional Medicine ◽  
Radical Scavenging Properties

Stachys schtschegleevii Sosn. (Lamiaceae alt. Labiatae) is one of 34 Stachys species found in Iran, and is morphologically similar to Stachys inflata. This plant has been used in the Iranian traditional medicine as a remedy for bacterial infections, rheumatic fever and other inflammatory conditions. The n-hexane, dichloromethane (DCM) and methanol (MeOH) extracts of the non-flowering aerial parts of this plant were assessed for their antibacterial and freeradical- scavenging activities using the micro-titer-based antimicrobial assay incorporating resazurin as an indicator of cell growth and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, respectively. While the n-hexane extract did not show any free-radical-scavenging activity, the MeOH extract showed the highest level of activity with a RC50 value of 2.94 x 10-2 mg/mL, which was about ten-fold less active than the positive control Trolox? (RC50 = 2.60 x 10-3 mg/mL). None of the extracts showed any antibacterial property against Bacillus cereus and B. subtilis at test concentrations. However, all extracts were active against ampicillin-resistant Escherichia coli and Staphylococcus aureus. The MeOH extract was the most potent (MIC range 1.56-6.25 mg/mL) among the extracts and was most active against ampicillin-resistant E. coli (MIC = 1.56 mg/mL).

scholarly journals EVALUATION OF ANTIOXIDANT AND FREE RADICAL SCAVENGING ACTIVITIES OF DIFFERENT SOLVENT EXTRACTS OF LEAVES OF PIPER UMBELLATUM

2017 ◽  
Vol 10 (2) ◽  
pp. 274
Author(s):  
Sethupandian Geetha ◽  
Kokkaiah Irulandi ◽  
Palanichamy Mehalingam
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Frap Assay ◽  
Radical Scavenging Properties

Objective: This study was designed to determine the Total phenol, flavonoid content, antioxidant and free radical scavenging properties of different solvent extracts of Piper umbellatum.  Methods: Different solvent extracts evaluated with DPPH radical scavenging activity and Reducing power activity.Results: The presence of phenol and flavonoid showed highly in the methanol extracts than ethyl acetate and acetone extracts. All the extracts have various level of antioxidant activity. Methanol solvent extract have good extraction and show significant antioxidant activity. The effect of reducing power of methanol extract revealed good antioxidant activity compare with other tested extracts.Conclusion: On the basis of the above results we concluded that methanol extract of Piper umbellatum whole plant extracts shows significant antioxidant activity than ethyl acetate and acetone extracts.Keywords: Piper umbellatum, Antioxidant, DPPH, FRAP assay, Polar, Non polar solvents

Composition of Dithyrea wislizenii fruit extract and free-radical scavenging activity of its constituents

2012 ◽  
Vol 90 (8) ◽  
pp. 652-659 ◽  
Author(s):  
Sabine Montaut ◽  
Julie Grandbois ◽  
Laura S. Rossi ◽  
Sonia Kamal ◽  
James Khouri ◽  
...  
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Positive Control ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Activity Assay ◽  
Fruit Extract ◽  
Brine Shrimp Lethality Assay

Glucolesquerellin (2), glucohesperin (3), quercetin 3-O-sophoroside (4), and quercetin 3-O-sophoroside-7-O-glucoside (5), isolated from the fruit of Dithyrea wislizenii , were quantified by HPLC. The fruit extract and flavonoids were not found to be toxic by using a brine shrimp lethality assay. The fruit extract and the flavonoids and glucosinolates were submitted to a free-radical scavenging activity assay with the diphenylpicrylhydrazyl radical (DPPH•). The concentration of quercetin (6) (a positive control for the flavonoids) able to scavenge 50% of DPPH• (SC50) was 32 ± 2 µmol/L (or 4 ± 1 µg/mL), which was about 27 times more potent than the crude extract. Compounds 4 and 5 had a SC50, the concentration of the compound required to scavenge 50% of the DPPH•, of 78 ± 1 µmol/L and 113 ± 10 µmol/L, respectively. The positive control for the glucosinolates, glucoraphasatin, (1) had a SC50 of 1768 ± 60 µmol/L. The glucosinolates 2 and 3 had a SC50 of 7819 ± 1968 and 970 ± 63 µmol/L, respectively.

scholarly journals Free radical scavenging activity of ethanolic extracts from herbs and spices commercialized in Brazil

2008 ◽  
Vol 51 (6) ◽  
pp. 1225-1232 ◽  
Author(s):  
Lilian Regina Barros Mariutti ◽  
Gisela Pizarro de Mattos Barreto ◽  
Neura Bragagnolo ◽  
Adriana Zerlotti Mercadante
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Black Pepper ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Decay Rate Constant ◽  
Ethanolic Extracts ◽  
Radical Scavenging Properties ◽  
Herbs And Spices

Ethanolic extracts from 23 different dried herbs and spices commercialized in Brazil were investigated for their free radical scavenging properties using the stable free radicals 2,2'-diphenyl-β-picrylhydrazyl (DPPH•) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS•+), and Trolox as reference (TEAC) for both radicals. The kinetic curves for both radicals showed to follow the first order kinetics model and the decay rate constant (k obs) was calculated. For all the samples, the two methods showed a good linear TEAC correlation, indicating that the average reactivity of the compounds present in the ethanolic extracts was similar. Sage and rosemary extracts showed the highest free radical scavenging capacities, while onion showed negligible activity and colorifico, one of the most consumed spices in Brazil, showed low ABTS•+ scavenging activity. Three distinct situations were found for the extracts concerning the DPPH• scavenging capacities: (1) extracts, like rosemary and laurel, that presented the same efficient concentrations (EC50 ) but differed in the TEAC values and velocities of action (k obs), (2) extracts, such as garlic and basil, that showed similar EC50 and TEAC values, but different k obs values and (3) extracts that reacted at the same velocities but completely differed in the free radical scavenging capacities, like black pepper, savory, nutmeg, rosemary and sage. Similar considerations could be done for the ABTS•+ results. For the first time the ABTS•+ scavenging activity for allspice, basil, cardamom, chives, colorifico, cumin, dill, laurel, marjoram, parsley and tarragon was reported.

scholarly journals Insecticidal Activity and Free Radical Scavenging Properties of Isolated Phytoconstituents from the Saudi Plant Nuxia oppositifolia (Hochst.)

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 914
Author(s):  
Shaza M. Al-Massarani ◽  
Ali A. El-Gamal ◽  
Adnan J. Al-Rehaily ◽  
Ebtesam S. Al-Sheddi ◽  
Mai M. Al-Oqail ◽  
...  
Keyword(s):  
Free Radical ◽  
Insecticidal Activity ◽  
Radical Scavenging ◽  
Positive Control ◽  
Free Radical Scavenging ◽  
Alcoholic Extract ◽  
Chemical Structures ◽  
Aerial Parts ◽  
Radical Scavenging Properties ◽  
Promising Source

Chromatographic purification of the alcoholic extract from the aerial parts of the Saudi plant Nuxia oppositifolia (Hochst.), Benth., resulted in five isolated phenolic compounds. Two flavones, hispidulin (1) and jaceosidin (2), and the phenylethanoid glycosides, verbascoside (3), isoverbascoside (4), and conandroside (5), were identified and their chemical structures were determined by spectroscopic analyses. The insecticidal activity of compounds 1 and 2, in addition to 11 compounds isolated in a previous research (6–16), was evaluated against the Yellow Fever mosquito, Aedes aegypti. Four compounds displayed adulticidal activity with LD50 values of 2–2.3 μg/mosquito. Free radical scavenging properties of the plant extracts and compounds (1–5) were evaluated by measuring the 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonate radical cation (ABTS•+) scavenging activity. All compounds exhibited notable activity, compared with the positive control, l-Ascorbic acid. This study suggests that N. oppositifolia could be a promising source of secondary metabolites, some with lethal adulticidal effect against Ae. aegypti.

scholarly journals Morin Protects Human Respiratory Cells from PM2.5 Induced Genotoxicity by Mitigating ROS and Reverting Altered miRNA Expression

2019 ◽  
Vol 16 (13) ◽  
pp. 2389 ◽  
Author(s):  
Indhumathi Veerappan ◽  
Senthil Kumar Sankareswaran ◽  
Rajaguru Palanisamy
Keyword(s):  
Dna Damage ◽  
Free Radical ◽  
Mirna Expression ◽  
Radical Scavenging Activity ◽  
Fine Particulate Matter ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Oxidative Potential ◽  
Respiratory Cells ◽  
Radical Scavenging Properties

Chronic fine particulate matter (PM2.5) exposure causes oxidative stress and leads to many diseases in human like respiratory and cardiovascular disorders, and lung cancer. It is known that toxic responses elicited by PM2.5 particles depend on its physical and chemical characteristics that are greatly influenced by the source. Dietary polyphenolic compounds that possess antioxidant and free radical scavenging properties could be used for therapeutic or preventive approaches against air pollution related health hazards. This study evaluates characteristics and toxicity of PM2.5 collected from rural, urban, industrial, and traffic regions in and around Coimbatore City, Tamilnadu, India. Traffic PM2.5 particles contained higher amounts of metals and polycyclic aromatic hydrocarbons (PAHs). It also possessed higher levels of oxidative potential, induced more intracellular reactive oxygen species (ROS), and caused more levels of cell death and DNA damage in human respiratory cells. Its exposure up regulated DNA damage response related miR222, miR210, miR101, miR34a, and miR93 and MycN and suppressed Rad52. Pre-treatment with morin significantly decreased the PM2.5 induced toxicity and conferred protection against PM2.5 induced altered miRNA expression. Results of this study showed that cytoprotective effect of morin is due to its antioxidative and free radical scavenging activity.

scholarly journals Preparation, Characterization and Antioxidant Evaluation of Cu(II) and Zn(II) Tannates

2018 ◽  
Vol 5 (1) ◽  
pp. 158-171
Author(s):  
Gessyca G. Costa ◽  
Camila C.S.M. Brito ◽  
Ailton J. Terezo ◽  
Adriana P. Cardoso ◽  
Elias Y. Ionashiro ◽  
...  
Keyword(s):  
Free Radical ◽  
Metal Ions ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Hydroxyl Group ◽  
Scavenging Activity ◽  
Radical Scavenging Properties ◽  
Dsc Curves

Introduction: The interest in the antioxidant materials increased due to their free radical scavenging properties avoiding various pathologies. Tannic Acid (TA) is known to have high free radical scavenging activity, but its application is still limited. This paper had the objective of synthesis, characterization and evaluation of free radical scavenging activity of TA associated with Cu (II) and Zn (II) ions, in a buffered aqueous medium. Methods and Materials: Techniques of FTIR, simultaneous TG-DSC characterized the obtained compounds, and the evaluation of free radical scavenging activity was made using 2,2–diphenyl–1–picrylhydrazyl (DPPH) radical. The FTIR analysis of the compounds showed significant displacement of the main bands in the TA spectrum (νC=O, δO-H, σO-H), suggesting an association of metal ions with the oxygen of the hydroxyl group of TA. TG-DSC curves showed information about the thermal stability, stoichiometry, hydration water, and residues formation. Results and Conclusion: The TA association with the metal ions Cu(II) and Zn(II) decreased the antioxidant activity of the molecule significantly, showing that these compounds could be potentially used as pro-oxidant agents.

scholarly journals Free Radical Scavenging Capacity, Carotenoid Content, and NMR Characterization of Blighia sapida Aril Oil

2018 ◽  
Vol 2018 ◽  
pp. 1-7
Author(s):  
Andrea Goldson Barnaby ◽  
Jesse Clarke ◽  
Dane Warren ◽  
Kailesha Duffus
Keyword(s):  
Free Radical ◽  
Chemical Shifts ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Monounsaturated Fatty Acids ◽  
Free Radical Scavenging ◽  
Carotenoid Content ◽  
Nmr Characterization ◽  
Radical Scavenging Properties ◽  
Blighia Sapida

Blighia sapida aril oil is rich in monounsaturated fatty acids but is however currently not utilized industrially. The oil was characterized utilizing nuclear magnetic resonance (NMR) and Fourier Transform Infrared Spectroscopy (FTIR). A spectrophotometric assay was conducted to determine the free radical scavenging properties and carotenoid content of the oil. Chemical shifts resonating between δ 5.30 and 5.32 in the 1H NMR are indicative of olefinic protons present in ackee aril oil which are due to the presence of oleic acid. A peak at 3006 cm−1 in the FTIR spectra confirms the high levels of monounsaturation. The oil has a free radical scavenging activity of 48%  ± 2.8% and carotenoid content of 21 ± 0.2 ppm.

scholarly journals Bioactivity of Rumex obtusifolius (Polygonaceae)

2010 ◽  
Vol 62 (2) ◽  
pp. 387-392 ◽  
Author(s):  
Diane Harshaw ◽  
Lutfun Nahar ◽  
Brahmachari Vadla ◽  
Gadria Saif-E-Naser ◽  
Satyajit Sarker
Keyword(s):  
Free Radical ◽  
Solid Phase ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Bacterial Strains ◽  
Meoh Extract ◽  
Rumex Obtusifolius ◽  
Brine Shrimp Lethality Assay ◽  
Ethnobotanical Uses

Rumex obtusifolius L. (Polygonaceae), commonly known as 'broad-leaf dock', is one of the most common Irish wayside weeds, and it also occurs in silage fields, on river banks, in ditches and on waste grounds. The ethnobotanical uses of this species include its use as an antidote to nettle, depurative, astringent, laxative, and tonic, and in the treatment of sores, blisters, burns, cancer and tumors. The bioactivities of n-hexane, dichloromethane (DCM) and methanol (MeOH) extracts of the leaves of R. obtusifolius were assessed using the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assay, the newly developed micro-titer-based antimicrobial assay incorporating resazurin as an indicator of cell growth, and the brine shrimp lethality assay. The most potent free radical scavenging activity was displayed by the MeOH extract with a RC50 value of 7.80 x 10-2 mg/mL. Among the fractions obtained from solid-phase extraction (SPE) of the MeOH extract, the 50% aqueous methanolic SPE fraction exhibited the highest levels of free radical scavenging property (RC50 = 1.05 x 10-2 mg/mL). While the n-hexane extract did not show any antibacterial activity at test concentrations, the DCM extract was active only against Escherichia coli. However, the MeOH extract as well as the 50% and 80% SPE fractions of the MeOH extract showed significant antibacterial property against all bacterial strains tested. None of the extracts or fractions exhibited any significant toxicity towards brine shrimps.

Sign in / Sign up

Export Citation Format

Share Document