scholarly journals Mitotic activity of smooth muscle cells of the myoma: Does hormonal stimulation have an effect on the number of mitoses?

2010 ◽  
Vol 62 (1) ◽  
pp. 39-45
Author(s):  
Tatjana Kastratovic ◽  
Irena Tanaskovic ◽  
Vesna Lackovic ◽  
Marija Sorak ◽  
Vesna Stankovic ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Menstrual Cycle ◽  
Smooth Muscle Cells ◽  
Mitotic Activity ◽  
Luteal Phase ◽  
Muscle Cells ◽  
Secretory Phase ◽  
Hormonal Stimulation ◽  
Proliferative Phase ◽  
Proliferating Activity

Myomas develop as a result of increased mitotic (proliferating) activity of smooth muscle cells. In this study we examined the pathohistological samples of 176 myomas and their endometria that were obtained after hysterectomy from patients in the proliferative (follicular) and secretory (luteal) phase of the menstrual cycle. We examined the mitotic activity of the myoma cells in both phases and established that the average number of mitoses in the proliferative phase was significantly larger compared to the secretory phase, and that in the proliferative phase of the cycle there exists a statistically significant convergent association of the number of mitoses in the endometrium and in myomas. The number of endometrial mitoses is significantly larger than in myomas in both phases of the cycle.

scholarly journals The cannabinoid receptor CB1 affects the proliferation and apoptosis of adenomyotic human uterine smooth muscle cells of the junctional zone: a mechanism study

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Sha Wang ◽  
Bohan Li ◽  
Xue Shen ◽  
Hua Duan ◽  
Zhengchen Guo ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Menstrual Cycle ◽  
Smooth Muscle Cells ◽  
Untreated Control ◽  
Cannabinoid Receptor ◽  
Muscle Cells ◽  
Control Group ◽  
Untreated Control Group ◽  
Junctional Zone ◽  
Proliferation And Apoptosis

Abstract Background The denomyotic junctional zone (JZ) plays an important role in the pathogenesis of adenomyosis. Proliferating cell nuclear antigen (PCNA) is an important nuclear marker of cell proliferation. This study aimed to evaluate the effects of the cannabinoid receptor CB1 on proliferation and apoptosis in the JZ in women with and without adenomyosis. Methods JZ smooth muscle cells (JZSMCs) of the adenomyosis and control groups were collected and cultivated. Immunohistochemistry and immunoblotting were used for protein localization and expression detection of CB1 and PCNA. Additionally, qRT-PCR was used to quantitatively analyse the mRNA expression of the two. AM251 and ACEA were used to regulate the function of CB1 receptors, and CCK-8 assay and flow cytometry assay were used to verify the proliferation and apoptosis of JZSMCs after regulation. Results We demonstrated that in normal JZSMCs CB1 and PCNA messenger RNA (mRNA) and protein expression was significantly higher in the proliferative phase of the menstrual cycle than in the secretory phase. CB1 and PCNA expression in JZSMCs from women with ADS was significantly higher than that in control women and did not significantly differ across the menstrual cycle. CB1 receptor antagonist AM251 inhibited the proliferation of adenomyotic JZSMCs in a dose-dependent manner. The CB1 receptor agonist ACEA significantly promoted the proliferation of adenomyotic JZSMCs. The apoptosis rate of adenomyotic JZSMCs treated with AM251 was significantly higher than that of JZSMCs from the untreated control group. The apoptosis rate was significantly decreased in the ACEA group compared with that in the untreated control group. Furthermore, AM251 suppressed the phosphorylation of AKT and Erk1/2 in adenomyotic JZSMCs. The CB1 agonist ACEA significantly promoted the phosphorylation of AKT and Erk1/2. Conclusions Our results indicated that the levels of CB1 and PCNA were increased in patients with adenomyosis and that cyclic changes were lost. CB1 may affect uterine JZ proliferation and apoptosis in adenomyosis by enhancing AKT and MAPK/Erk signalling.

Enhanced Proliferating Activity of Cultured Smooth Muscle Cells From SHR

1989 ◽  
Vol 2 (2_Pt_1) ◽  
pp. 108-110 ◽  
Author(s):  
Jean-Luc Paquet ◽  
Maryvonne Baudouin-Legros ◽  
Pierre Marche ◽  
Philippe Meyer
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Proliferating Activity ◽  
Cultured Smooth Muscle Cells

Increased Mitotic Activity in Primary Cultures of Aortic Medial Smooth Muscle Cells after Exposure to Hyperlipemic Serum

1974 ◽  
pp. 193-195 ◽  
Author(s):  
Katti Fischer-Dzoga ◽  
Rose M. Jones ◽  
Dragoslava Vesselinovitch ◽  
Robert W. Wissler
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Mitotic Activity ◽  
Primary Cultures ◽  
Muscle Cells

Immunogold localization of HMB-45 antigen in pulmonary lymphangiomyomatosis

1995 ◽  
Vol 53 ◽  
pp. 998-999
Author(s):  
J.M. Minda ◽  
E. Dessy ◽  
G. G. Pietra
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Room Temperature ◽  
Osmium Tetroxide ◽  
Muscle Cells ◽  
Ultrastructural Localization ◽  
Reproductive Age ◽  
Thin Sections ◽  
Smooth Muscle Proliferation ◽  
Hmb 45

Pulmonary lymphangiomyomatosis (PLAM) is a rare disease occurring exclusively in women of reproductive age. It involves the lungs, lymph nodes and lymphatic ducts. In the lungs, it is characterized by the proliferation of smooth muscle cells around lymphatics in the bronchovascular bundles, lobular septa and pleura The nature of smooth muscle proliferation in PLAM is still unclear. Recently, reactivity of the smooth muscle cells for HMB-45, a melanoma-related antigen has been reported by immunohistochemistry. The purpose of this study was the ultrastructural localization of HMB-45 immunoreactivity in these cells using gold-labeled antibodies.Lung tissue from three cases of PLAM, referred to our Institution for lung transplantation, was embedded in either Poly/Bed 812 post-fixed in 1% osmium tetroxide, or in LR White, without osmication. For the immunogold technique, thin sections were placed on Nickel grids and incubated with affinity purified, monoclonal anti-melanoma antibody HMB-45 (1:1) (Enzo Diag. Co) overnight at 4°C. After extensive washing with PBS, grids were treated with Goat-anti-mouse-IgG-Gold (5nm) (1:10) (Amersham Life Sci) for 1 hour, at room temperature.

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