scholarly journals Genetic variability within Serbian populations of the rare and endangered pottioid moss Hilpertia velenovskyi (Schiffn.) Zander inferred by isozyme analyses

2008 ◽  
Vol 60 (2) ◽  
pp. 207-213 ◽  
Author(s):  
M. Sabovljevic ◽  
Aneta Sabovljevic ◽  
J. Radulovic ◽  
Ivana Dragicevic

Genetic variability within Serbian populations of the pottioid moss Hilpertia velenovskyi (Schiffn.) Zander was studied. Eight populations of this rare and endangered moss species were chosen for peroxidase isozyme analyses. From the data obtained, it can be inferred that four haplotypes are present among H. velenovskyi populations in Serbia, the Banat population being distinctive from all others.

Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1887
Author(s):  
Natalia V. Dementieva ◽  
Andrei A. Kudinov ◽  
Tatiana A. Larkina ◽  
Olga V. Mitrofanova ◽  
Artyom P. Dysin ◽  
...  

Preserving breed uniqueness and purity is vitally important in developing conservation/breeding programs for a germplasm collection of rare and endangered chicken breeds. The present study was aimed at analyzing SNP genetic variability of 21 small local and imported purebred and F1 crossbred populations and identifying crossbreeding events via whole-genome evaluation of runs of homozygosity (ROH). The admixture models more efficiently reflected population structure, pinpointing crossbreeding events in the presence of ancestral populations but not in their absence. Multidimensional scaling and FST-based analyses did not discriminate properly between purebred populations and F1 crossbreds, especially when comparing related breeds. When applying the ROH-based approach, more and longer ROHs were revealed in purebred individuals/populations, suggesting this as an effective implement in genome-wide analysis of germplasm breed purity.


Biologia ◽  
2008 ◽  
Vol 63 (6) ◽  
Author(s):  
Marko Sabovljević ◽  
Jan-Peter Frahm

AbstractThe genetic structure, diversity and phylogeography of the moss species Campylopus oerstedianus in Europe was studied, based on the ITS region of the nrDNA of nine selected European populations. Although this species is only known in sterile stage, long-distance dispersal and gene flow among populations seem to be present within Europe. High levels of genetic differentiation between the investigated Greek population and the western European populations indicate a long lasting isolation.The populations from France and Switzerland root together and supposedly have a common origin. From the French populations, the one from the Pyrenees is the most basal one and the populations in the Massif Central and the Vosges Mts can be derived from it. This indicates relatively recent dispersal of the species from the Mediterranean to the north, in spite of the lack of sporophytes and the fact that the type of the dispersed propagules and their vectors are not obvious.


1988 ◽  
Vol 66 (8) ◽  
pp. 1621-1631 ◽  
Author(s):  
A. M. Desrochers ◽  
J. F. Bain ◽  
S. I. Warwick

The Carduus nutans L. complex in North America has been treated either as one species with four subspecies (ssp. nutans, ssp. leiophyllus (Petrovic) Stoj. & Stef., ssp. macrolepis (Peterm.) Kazmi, and ssp. macrocephalus (Desf.) Nyman) or as three species: Carduus nutans with two subspecies (ssp. nutans and ssp. macrolepis), C. thoermeri Weinm., and C. macrocephalus Desf. A biosystematic study of this complex, including morphological, flavonoid, and isozyme analyses, of 19 populations, was conducted to clarify the taxonomy of this complex in Canada. Both the morphological and flavonoid analyses clearly indicate the existence of only two closely related groups of taxa referable to ssp. nutans and ssp. leiophyllus. The classificatory discriminant analysis indicated correct classification rates of individuals of 93.6% and 96.0% for ssp. nutans and ssp. leiophyllus, respectively. Each taxon has a distinct flavonoid profile. Given the high mean genetic identity value (ī = 0.93) between the two taxa in the complex, and the estimates of genetic variability obtained, the taxa are best treated at the subspecific level, as ssp. nutans and ssp. leiophyllus.


2018 ◽  
Vol 10 (1) ◽  
pp. 1
Author(s):  
Muhammad Restu ◽  
Mohammad Naiem

A research was conducted to determine genetic variability and structure of ebony either within provenances or within trees in the same provenance using isozyme analyses. Results of this study are expected to show genetic variability of ebony with different provenances. For the purpose of the study, five provenances of ebony (Maros,Barru, Sidrap, Malili, and Mamuju) were prepared. The isozyme analysis using electrophoresis was applied to determine the genetic variability. The obtained data were statistically analyzed using multivariate and dendrogram analyses with Numerical Taxonomy System (NTSYS) Program applying unwighted pairgroup method and arrithmetic average (UPGMA) approach. Results showed that the variability of genetic provenances of ebony were less than any other tree species. Among the existing provenances, Barru dan Mamuju showed higher genetic variability compared to other provenances. Ebony provenance was generally found to homozygously increase or to performinbreeding. The genetic variability of ebony was mostly derived from the variability in population (95.4%). Grouping individual trees based on their provenances indicated that Malili provenance showed closed relationship to Maros provenance, whilst Mamuju provenance showed closed relationship to Barru and Sidrap provenances.


HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 441C-441
Author(s):  
Luz Marina Reyes ◽  
Orlando Martinez ◽  
Margarita Beltran

Fifteen clones of banana and plantain of different ploidies, belonging to the Colombian Collection of Musaceas (CCM), maintained at in vitro conditions were characterized. Twenty-three isozyme systems were analyzed using young leaves of micropropagated clones. Eleven systems presented electrophoretic activity: diaphorase (DIA), esterase (EST), glutamate dehydrogenase (GDH), malate dehydrogenase (MDH), malic enzyme (ME), peroxidase (PRX), phosphoglucoisomerase (PGI), phosphogluconate dehydrogenase (PGDH), phosphoglucomutase (PGM), ribulose biphosphate carboxilase (RUB), and shikimate dehydrogenase (SKDH). DIA and RUB isozymes are reported the first time for the genus Musa, and ME, GDH, PGDH, and PGI are not reported previously in acrilamide support. A total of 24 loci were identified that encoded at least 50 alleles. The enzymes with greater genetic variability were EST and DIA, with 14 and 10 alleles, respectively—these represent 48% of the polymorphism detected in this study. The systems PGM, SKDH, PGDH, and ME allowed to differentiate clones of M. acuminata (bananas AA and AAA) from hybrid clones derived of M. acuminata × M. balbisiana (plantains AAB and ABB). Otherwise, it was found that materials maintained under in vitro conditions for more than 10 subcultures presented evidence of variation at the protein level. The isozymes that allowed us to observe these changes were: DIA, EST, ME, PGDH, PGM, and SKDH.


HortScience ◽  
2012 ◽  
Vol 47 (9) ◽  
pp. 1347-1350 ◽  
Author(s):  
Marko Sabovljević ◽  
Milorad Vujičić ◽  
Jasmina Šinžar Sekulić ◽  
Jose Gabriel Segarra-Moragues ◽  
Beata Papp ◽  
...  

This study provides the results of the developmental biology of the highly rare and endangered moss species Bruchia vogesiaca (recorded in less than 30 localities in the Northern Hemisphere, mainly western, central, and southwestern Europe). The aim of the study was to achieve the fully developed gametophyte and to propagate it for the purpose of conservation, reintroduction, and introduction to potential habitats free from xenic contamination. These gametophytes will be used for the study of genetics and genomics of this species. The micropropagation of B. vogesiaca was successfully applied on BCD medium supplemented with 0.1 μM BA and on BCD supplemented with 0.3 μM IBA and 0.3 μM BA for numerous gametophore production. The highest production of secondary protonema was achieved on MS/2 S/2 medium enriched with 0.1 or 0.3 μM IBA and 0.3 μM BA. Rather successfully applied micropropagation of this threatened moss species enables better knowledge of its biology and is of great value for its conservation biology and developmental research. Chemical names used: indole-3-butyric acid (IBA), N6-benzyladenine (BA), Murashige and Skoog medium (MS).


2001 ◽  
Vol 21 (6) ◽  
pp. 580-592 ◽  
Author(s):  
Arnold Boonstra ◽  
Dick de Zeeuw ◽  
Paul E. de Jong ◽  
Gerjan Navis

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