scholarly journals Effect of medium pH and cultivation period on mycelial biomass, polysaccharide, and ligninolytic enzyme production by Ganoderma lucidum from Montenegro

2006 ◽  
Vol 58 (3) ◽  
pp. 179-182 ◽  
Author(s):  
Jelena Vukojevic ◽  
Mirjana Stajic ◽  
Sonja Duletic-Lausevic ◽  
Jasmina Simonic
Keyword(s):  
Ganoderma Lucidum ◽  
Enzyme Production ◽  
Ligninolytic Enzymes ◽  
Extracellular Polysaccharide ◽  
Ligninolytic Enzyme ◽  
Extracellular Polysaccharides ◽  
Medium Ph ◽  
Polysaccharide Production ◽  
Intracellular Polysaccharide ◽  
Maximal Production

The effect of initial medium pH on biomass, extracellular and intracellular polysaccharide, and ligninolytic enzyme production by Ganoderma lucidum was investigated at different pH values after 7 and 14 days of cultivation. Maximal production of biomass was recorded at pH 4.5 and 5.0; maximal production of extracellular polysaccharides at pH 7.0 and 3.0; and maximal production of intracellular polysaccharides at pH 7.0 and 5.5. Ligninolytic enzymes were not produced at any pH of the medium. Maximal biomass production was obtained on the 11th day of cultivation; maximal extracellular polysaccharide production on the 7th day; and maximal intracellular polysaccharide production on the 6th and 10th day of cultivation. .

Effects of Fluoride, Lithium and Strontium on Extracellular Polysaccharide Production by Streptococcus mutans and Actinomyces viscosus

1981 ◽  
Vol 60 (C) ◽  
pp. 1601-1610 ◽  
Author(s):  
Patrick Treasure
Keyword(s):  
Trace Elements ◽  
Streptococcus Mutans ◽  
Extracellular Polysaccharide ◽  
Water Soluble ◽  
Extracellular Polysaccharides ◽  
Polysaccharide Production

Effects of trace elements on production of extracellular polysaccharides (EPS) by S. mutans and A. viscosus were examined in vitro. Fluoride enhanced EPS production. Lithium and strontium had little effect alone, but tended to reverse the effect of fluoride. The proportion of water-soluble EPS and the proportion of glucosyl-EPS were increased by fluoride.

scholarly journals Ligninolytic Enzyme Production and Decolorization Capacity of Synthetic Dyes by Saprotrophic White Rot, Brown Rot, and Litter Decomposing Basidiomycetes

2020 ◽  
Vol 6 (4) ◽  
pp. 301
Author(s):  
Ivana Eichlerová ◽  
Petr Baldrian
Keyword(s):  
Enzyme Production ◽  
Ligninolytic Enzymes ◽  
Brown Rot ◽  
Dye Decolorization ◽  
Ligninolytic Enzyme ◽  
White Rot ◽  
Laccase Production ◽  
Synthetic Dyes ◽  
Mn Peroxidase ◽  
Taxonomic Groups

An extensive screening of saprotrophic Basidiomycetes causing white rot (WR), brown rot (BR), or litter decomposition (LD) for the production of laccase and Mn-peroxidase (MnP) and decolorization of the synthetic dyes Orange G and Remazol Brilliant Blue R (RBBR) was performed. The study considered in total 150 strains belonging to 77 species. The aim of this work was to compare the decolorization and ligninolytic capacity among different ecophysiological and taxonomic groups of Basidiomycetes. WR strains decolorized both dyes most efficiently; high decolorization capacity was also found in some LD fungi. The enzyme production was recorded in all three ecophysiology groups, but to a different extent. All WR and LD fungi produced laccase, and the majority of them also produced MnP. The strains belonging to BR lacked decolorization capabilities. None of them produced MnP and the production of laccase was either very low or absent. The most efficient decolorization of both dyes and the highest laccase production was found among the members of the orders Polyporales and Agaricales. The strains with high MnP activity occurred across almost all fungal orders (Polyporales, Agaricales, Hymenochaetales, and Russulales). Synthetic dye decolorization by fungal strains was clearly related to their production of ligninolytic enzymes and both properties were determined by the interaction of their ecophysiology and taxonomy, with a more relevant role of ecophysiology. Our screening revealed 12 strains with high decolorization capacity (9 WR and 3 LD), which could be promising for further biotechnological utilization.

scholarly journals GANODERMA LUCIDUM: A POTENT SOURCE OF LIGNINOLYTIC ENZYME PRODUCTION.

2017 ◽  
Vol 5 (5) ◽  
pp. 1977-1981 ◽  
Author(s):  
Nikki Agrawal ◽  
◽  
Preeti Verma ◽  
RaviShankar Singh ◽  
SushilKumar Shahi ◽  
...  
Keyword(s):  
Ganoderma Lucidum ◽  
Enzyme Production ◽  
Ligninolytic Enzyme

scholarly journals Effect of Pretreated Colza Straw on the Growth and Extracellular Ligninolytic Enzymes Production by Lentinula edodes and Ganoderma lucidum

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 157
Author(s):  
Galina Makarenkova ◽  
Valda Balode ◽  
Dzintra Zala ◽  
Elina Azena ◽  
Alexander Rapoport ◽  
...  
Keyword(s):  
Ganoderma Lucidum ◽  
Laccase Activity ◽  
Lentinula Edodes ◽  
Culture Media ◽  
Agricultural Waste ◽  
Ligninolytic Enzymes ◽  
Ligninolytic Enzyme ◽  
Solid Media ◽  
Extracellular Laccase ◽  
Surface Liquid

Lentinula edodes 3565 and Ganoderma lucidum 9621 were compared for their ability to produce lignocellulolytic enzymes in submerged (SM) and surface liquid (SL) fermentation of hydrolysed colza straw lignin waste that remained after the production of furfural and bioethanol (CS lignin). Application of cultivated mushrooms to dispose of pretreated colza straw agricultural waste is an approach to decrease the quantity of residual lignin while simultaneously obtaining active substances, e.g., the ligninolytic enzyme complex from mycelium. The effect of adding CS lignin to culture media on the yield of L. edodes and G. lucidum mycelium and extracellular laccase activity was studied. It was revealed that the mycelial growth of G. lucidum on solid media was significantly improved by adding CS lignin. Laccase activity during SL cultivation of L. edodes on medium with CS lignin gradually increased over the experiment starting on day 21 and peaked at 520 U/mL on day 28. G. lucidum expressed the maximum laccase activity, 540 U/mL, during the first 14 days of mycelium SM cultivation. Extracellular laccase activity was enhanced about 35- to 40-fold at cultivation of L. edodes and about 10- to 15-fold in the case of G. lucidum by supplementing liquid culture media with CS lignin.

scholarly journals Screening of ligninolytic fungi for bioremediation of dyes

2021 ◽  
pp. 35-42
Author(s):  
Seri Amelie Muliyadi ◽  
Elida Tengku Zainal Mulok ◽  
Noor Hana Hussein ◽  
Rohana Mat Nor
Keyword(s):  
Water Pollution ◽  
Textile Industry ◽  
Enzyme Production ◽  
Dna Isolation ◽  
Ligninolytic Enzymes ◽  
Its Region ◽  
Soft Rot ◽  
Ligninolytic Enzyme ◽  
Fungal Species ◽  
Ligninolytic Fungi

Water pollution is a growing concern worldwide. One of the main causes of water pollution includes the textile industry which produces a large amount of wastewater every day. This wastewater is known to contain dyes that are recalcitrant and hard to treat. In order to solve this problem, bioremediation using ligninolytic fungi is commonly used for the ligninolytic enzymes which are able to break down the dyes. In this study, samples were collected from decaying woods and soils in the vicinity of UiTM Puncak Alam forests. A total of 20 fungal isolates were tested for ligninolytic enzyme production. Out of the 20 isolates, 13 were found to produce lignin peroxidase and manganese peroxidase, but only one produced laccase. The isolate that produced all three enzymes was used for DNA isolation and identified using amplification of the ITS region by PCR. The isolate was identified as Trichoderma asperellum, a soft rot fungal species which is renowned for its role in bioremediation as a biosorbent.

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