scholarly journals Establishment and development of the Catherine’s moss Atrichum undulatum (Hedw.) P. Beauv. (Polytrichaceae) in in vitro conditions

2006 ◽  
Vol 58 (2) ◽  
pp. 87-93 ◽  
Author(s):  
Aneta Sabovljevic ◽  
Tijana Cvetic ◽  
M. Sabovljevic
Keyword(s):  
In Vitro Culture ◽  
Ms Medium ◽  
Mineral Salts ◽  
Anatomical Characteristics

The effect of sucrose and mineral salts on morphogenesis of the Catherine?s moss (Atrichum undulatum) in in vitro culture was tested. In vitro culture of this species was established from disinfected spores on Murashige and Skoog (MS) medium. Apical shoots of gametophytes were used to investigate the influence of sucrose and mineral salts on protonemal and gametophyte growth and multiplication. Paper also treats morpho-anatomical characteristics of plants grown in nature and plants derived from in vitro culture.

scholarly journals In vitro culture and apogamy: Alternative pathway in the life cycle of the moss Amblystegium serpens (Amblystegiaceae)

2005 ◽  
Vol 57 (4) ◽  
pp. 267-272 ◽  
Author(s):  
Tijana Cvetic ◽  
Marko Sabovljevic ◽  
Aneta Sabovljevic ◽  
D. Grubisic
Keyword(s):  
Life Cycle ◽  
In Vitro Culture ◽  
Alternative Pathway ◽  
Ms Medium ◽  
Air Humidity ◽  
Mineral Salts ◽  
First Report ◽  
Macro Mineral

In vitro culture of the moss Amblystegium serpens (Amblystegiaceae) was established on hormone-free Murashige and Skoog (MS) medium that contained a half amount of MS micro- and macro- mineral salts and vitamins, 100 mg/l myoinositol, 30 g/l sucrose, and 0.70% (w/v) agar. Spores were germinated and primary protonema developed on the above medium at 16 h day/8 h night 25?2?C, 60-70% air humidity, and irradiance of 47 ?mol/m2s. Three months after development of primary protonema, seven sporophytes appeared directly from primary protonema without generation alternation. The phenomenon of apogamous sporophyte formation is very rare, both in nature and under in vitro conditions. This is the first report of apogamy induced by Amblystegium serpens.

scholarly journals In vitro bulblet regeneration from immature embryos of endangered and endemic Muscari azureum

2011 ◽  
Vol 63 (1) ◽  
pp. 209-215 ◽  
Author(s):  
S. Uranbey
Keyword(s):  
Callus Induction ◽  
High Frequency ◽  
Ornamental Plant ◽  
Immature Embryos ◽  
Induction Medium ◽  
Ms Medium ◽  
Culture Initiation ◽  
Mineral Salts ◽  
Font Color

A high frequency of bulblet regeneration was achieved for the endemic and endangered ornamental plant Muscari azureum using immature embryos. Immature embryos of M. azureum were cultured on a callus induction medium consisting of N6 mineral salts and vitamins, 400 gL-1 casein + 40 gL-1 sucrose + 2 mgL-1 L-proline, 2 mgL-1 2,4-D and 2 gL-1 Gelrite. Then the embryogenic callus clusters were transferred to a bulblet induction medium consisting of MS mineral salts and vitamins containing different concentrations and combinations of BAP, KIN, TDZ, Zeatin, IAA, NAA, 30 gL-1 sucrose and 7 gL-1 agar. Prolific bulblet multiplication (over 13 bulblets/embryo) was achieved from immature embryos after 5-6 months of culture initiation. Well-developed bulblets were excised and individually rooted on ? strength MS medium supplemented with 1 mgL-1 IBA, 0.5 gL-1activated charcoal, 20 gL-1sucrose and 6 gL-1agar and acclimatized. <br><br><font color="red"><b> This article has been retracted. Link to the retraction <u><a href="http://dx.doi.org/10.2298/ABS150608072E">10.2298/ABS150608072E</a><u></b></font>

In vitro culture of pointed gourd (Trichosanthes dioica Roxb.)

1970 ◽  
Vol 35 (1) ◽  
pp. 135-142 ◽  
Author(s):  
MA Malek ◽  
D Khanam ◽  
M Khatun ◽  
MH Molla ◽  
MA Mannan
Keyword(s):  
Plant Regeneration ◽  
In Vitro Culture ◽  
Culture Media ◽  
Culture Conditions ◽  
Root Induction ◽  
Ms Medium ◽  
Trichosanthes Dioica ◽  
Pointed Gourd ◽  
Regenerated Plantlets

An experiment was conducted to study the in vitro culture of pointed gourd. Cotyledon rescued from physiologically matured seeds (PMS) and immatured seeds (IMS) of pointed gourd were used as explants. Cotyledon excised from PMS responded very well in all culture conditions. Plant regenerated from cotyledon of PMS ranged from 38 to 96% in different hormonal formulations of culture media. Highest percentage of shoot regeneration was observed in MS + 1.0 mg/l BAP and lowest in MS + 2.5 mg/l BAP. No plant regeneration was observed in cotyledon from immatured seeds. The highest percentage of root induction (99%) was achieved in half MS medium supplemented with 0.5 mg/l NAA. The regenerated plantlets were successfully established in earthen pot. Keywords: Cotyledon; in vitro; pointed gourd. DOI: 10.3329/bjar.v35i1.5874Bangladesh J. Agril. Res. 35(1) : 135-142, March 2010

scholarly journals In Vitro Culture of Heuchera villosa ‘Caramel’

HortScience ◽  
2017 ◽  
Vol 52 (4) ◽  
pp. 622-624 ◽  
Author(s):  
Hua Q. Zhao ◽  
Qing H. He ◽  
Li L. Song ◽  
Mei F. Hou ◽  
Zhi G. Zhang
Keyword(s):  
Acetic Acid ◽  
In Vitro Culture ◽  
Butyric Acid ◽  
Shoot Regeneration ◽  
Ms Medium ◽  
Shoot Induction ◽  
Induction Rate ◽  
Regenerated Plantlets ◽  
Indole 3 Acetic Acid

The procedure for Heuchera villosa ‘Caramel’ propagation was investigated, which involves shoot regeneration, rooting of regenerated shoots, and acclimation of regenerated plantlets. Petioles, as explants, were cultured on MS medium supplemented with 1-naphthylacetic acid (NAA), benzylaminopurine (BA), thidiazuron (TDZ) and callus formed on all media. Shoots were observed to proliferate from callus on media with BA and NAA, whereas no shoots regenerated on media with TDZ and NAA. On media containing 0.5 or 1.0 mg·L−1 BA in combination with NAA, the regenerated shoots showed severe hyperhydricity, whereas on media containing 0.1 mg·L−1 BA in combination with NAA, the regenerated shoots grew normally. The highest shoot induction rate, 90.6%, was obtained on media containing 0.1 mg·L−1 BA and 0.01 mg·L−1 NAA. The effects of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), and NAA on rooting of H. villosa ‘Caramel’ was explored. The highest rooting rate (95%) was obtained on 1/2 MS medium containing 0.2 mg·L−1 NAA. In the subsequent acclimation experiments, about 85% of rooted plantlets survived and grew normally.

scholarly journals 883 PB 279 REGENERATING ADVENTITIOUS PLANTS FROM IN VITRO CULTURE OF LIATRIS SPICATA (L.) WILLD. COTYLEDONS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 560d-560
Author(s):  
Dennis P. Stimart ◽  
John C. Mather
Keyword(s):  
In Vitro Culture ◽  
Adventitious Shoots ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Ms Medium ◽  
Ex Vitro ◽  
Adventitious Shoot Formation ◽  
Murashige Skoog ◽  
Micropropagated Plants

Cotyledons from developing embryos 6 to 8 weeks old of Liatris spicata (blazing star) were cultured on Murashige-Skoog (MS) medium containing 0, 0.4, 4.4, and 44.4 μ M benzyladenine (BA) or 0, 0.2, 2.2, and 22.2 μ M thidiazuron (TDZ) to induce adventitious shoot formation. The highest percent of cotyledons forming shoots with highest shoot counts was on medium containing 2.2 μ M TDZ. Vitreous shoots formed on medium with 22.2 μ M TDZ. Callus derived from cotyledons and cultured on medium containing 4.44 μ M BA or 2.2 μ M TDZ formed adventitious shoots with highest shoot counts on 4.44 μ M BA. Adventitious shoots derived from cotyledons and callus were rooted on MS medium with 5.0 μ Mindole-3-butyric acid, acclimatized and grown ex vitro. All micropropagated plants appeared similar to each other.

scholarly journals Regenerating Adventitious Shoots from in Vitro Culture of Liatris spicata (L.) Willd. Cotyledons

HortScience ◽  
1996 ◽  
Vol 31 (1) ◽  
pp. 154-155
Author(s):  
Dennis P. Stimart ◽  
John C. Mather
Keyword(s):  
In Vitro Culture ◽  
Adventitious Shoots ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Ms Medium ◽  
Adventitious Shoot Formation ◽  
Regenerated Plants ◽  
Murashige And Skoog Medium ◽  
Homogeneous Chemical

Cotyledons from developing 6- to 8-week-old embryos of Liatris spicata (L.) Willd. (blazing star) were cultured on Murashige and Skoog medium containing 0, 0.4, 4.4, or 44.4 μm BA or 0, 0.2, 2.2, or 22.2 μm TDZ to induce adventitious shoot formation. The highest percentage of cotyledons forming the most shoots was on medium containing 2.2 μm TDZ. Cotyledon-derived callus cultured on medium containing 4.4 μm BA formed ≈16 times more adventitious shoots than on 2.2 μm TDZ. Adventitious shoots derived from cotyledons or callus produced roots when placed on MS medium containing 5.0 μm IBA. Regenerated plants that flowered in the field appeared homogeneous. Chemical names used: N6-benzyladenine (BA), thidiazuron (TDZ), indole-3-butyric acid (IBA).

scholarly journals In Vitro Shoot Regeneration and Development of Microcorms of Moroccan Saffron (Crocus sativus L.)

2017 ◽  
pp. 50-55
Author(s):  
Khalid Lagram ◽  
Mohamed Ben El Caid ◽  
Souad El Aaouam ◽  
Mohamed Lachheb ◽  
Abdelhamid El Mousadik ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Culture Medium ◽  
Crocus Sativus ◽  
Induction Medium ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Male Sterile ◽  
Indirect Organogenesis ◽  
Crocus Sativus L

Crocus sativus L. is a male sterile vegetatively propagated plant. Its flower produces stigmas that when dried, constitute the source of a spice commonly known as Saffron. Slow vegetative propagation and diseases limit the production and the development of saffron. “In vitro” culture could be an effective method to overcome these limitations by improving the quantity and the quality of the planting materials. In this work, Crocus sativus L. segments corms of cultivar from the region of Taliouine (Southeast of Morocco) were used for the propagation through indirect organogenesis. To optimize the in vitro growth conditions, we have used the Murashige and Skoog medium (MS medium), supplemented with 2.4-dichlorophenoxyacetic acid (2.4-D) and with 6-benzylaminopurin (BAP) at combination of various concentrations. Our results showed the formation of callus in 85.42% of explants that grow in a culture medium supplemented with 2,4-D combined with BAP, at a concentration of 1mg/l each. In addition, we observed that increasing the concentration of BAP in the culture medium to 1.5mg/l improved the rate of shoots initiation (0.81). In the meantime, we noted that a combination of BAP (8mg/l) and Naphthalene acetic acid (NAA; 2mg/l) has significantly improved the rate of the formation of advanced shoots (6.65). Finally, the shoots that developed were transferred to an induction medium of roots and corms. As a result, we observed that 50% of shoots tested in ½ MS medium supplemented with 2.4-D and of BAP (1 mg/l each) and 5% sucrose, formed corms. Our study provides a first database for in vitro culture of Moroccan saffron cultivars.

scholarly journals Regeneration of Cucumis melo L. plants from cell suspension derived from hypocotyls callus

2013 ◽  
Vol 7 (3) ◽  
pp. 23-28
Author(s):  
Jamella Hazza Rasheed
Keyword(s):  
In Vitro Culture ◽  
Cell Suspension ◽  
Callus Culture ◽  
Cucumis Melo ◽  
Ms Medium ◽  
Regeneration Capability ◽  
Cucumis Melo L

This study aimed to investigate the regeneration capability of cell suspension derived from hypocotyl callus of the vegetable plant, muskmelon, Cucumis melo L. Culture of the densities (7.5, 9.8, 13.0, 11.2, 7.8, 4.2 )× 102 cell/ml produced callus primordia which formed typical callus culture successfully. This green – yellowish color and semi-compact callus regenerated shoots on agar solidified MS medium supplemented with 2.0mgL-1BA. These regenerates rooted readily in MS0 medium. They were adapted and routinely transferred to soil. The conclusion of this work that muskmelon plants have a good response to in vitro culture with no need to specific requirements.

scholarly journals Determinación histológica de regenerantes de Euchile mariae (Ames) Withner, (Orchidaceae), obtenidos a partir de protocormos cultivados in vitro

Lankesteriana ◽  
2015 ◽  
Vol 7 (1-2) ◽  
Author(s):  
Iris Suárez-Quijada ◽  
Estela Sandoval-Zapotilla ◽  
Mabel Hernández-Altamirano ◽  
Víctor Chávez-Ávila
Keyword(s):  
In Vitro Culture ◽  
Somatic Embryos ◽  
Histological Analysis ◽  
Leaf Primordium ◽  
Ms Medium ◽  
Modified Ms Medium ◽  
Histochemical Tests ◽  
Average Size

From the germination of seeds of Euchile mariae in modified MS medium, the formation of pro- tocorms was achieved. Once these reached an average size from 2 to 5 mm long and the formation of his first leaf primordium, they were used like explants to induce a morphogenetic response. Through in vitro culture of top and bottom protocorms sections, were obtained differentiated structures from asexual origin. Their morphology was similar to protocorms obtained from the germination of seeds, in this way we call them protocorm like bodies (PLB’s). Through of the histological analysis of these structures it was possible to reveal that these PLB’s turned out to be somatic embryos. The histochemical tests demonstrate the pres- ence of cellular contents like: proteins, lipids and starch; both in the cells of the embryos as well as in the cells of initial tissues. 

scholarly journals EFFICIENT in vitro PROPAGATION OF Amaranthus viridis L. USING NODE EXPLANTS

2020 ◽  
Vol 19 (4) ◽  
pp. 41-51
Author(s):  
Tour Jan ◽  
Ikram Ullah ◽  
Bilal Muhammad ◽  
_ Tariq ◽  
Ali Mansoor ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Ammonium Nitrate ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Dark Green

Hyperhydricity is a frequently problem in plants during in vitro culture and affected micropropagation ofplants. To develop an efficient in vitro regenerated system without hyperdydricity, we demonstrated the effectof different disinfected agents (mercuric chlorite and hypochlorite), growth regulators, their concentrationsand combinations, Agar, pH, ammonium nitrate (NH4NO3) and number of subcultures. Mercuric chlorite at0.07% and exposing time (9–10 min) was appropriate for hygienic culture. The shoots induced by Benzyladnine(BA) alone or in combination with α-Naphthaleneacetic acid (NAA) exhibited maximum multiplicationwith symptoms of hyperhydricity than those induced by Kinetin alone or in combination with NAA. Hyperhydricitywas also reduced by increasing the concentration of agar, pH and elimination of NH4NO3 from themacroelements of Murashig and Skoog (MS) medium. Repeated subcultures affected both multiplication andhyperhydricity. The multiplication of shoots increased from parental culture up to 5th subculture and thereafterdeclined in 6th subculture. Although shoot hyperhydricity were observed from 1st subculture (19%) andthen increased up to 85% in 6th subculture. This increased in hyperhydricity could be due to the remaininginfluence of hormones. In shoots of 5th subculture the content of chlorophyll (dark green) were higher thanshoots of 6th subculture.

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