scholarly journals Effects of CaMKII Regulation on Atrial Action Potential Under Oxidative Stress Condition

2018 ◽  
Author(s):  
Haibo Sui ◽  
Qince Li ◽  
Kuanquan Wang ◽  
Funebi Francis Ijebu ◽  
Yongfeng Yuan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Action Potential ◽  
Stress Condition ◽  
Oxidative Stress Condition

scholarly journals L-Carnitine: An Antioxidant Remedy for the Survival of Cardiomyocytes under Hyperglycemic Condition

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Fernanda Vacante ◽  
Pamela Senesi ◽  
Anna Montesano ◽  
Alice Frigerio ◽  
Livio Luzi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Mitochondrial Dysfunction ◽  
Signaling Pathways ◽  
Stress Condition ◽  
Cardiac Cells ◽  
Ros Production ◽  
Protective Effects ◽  
H9c2 Cells ◽  
Rat Cardiomyocytes ◽  
Oxidative Stress Condition

Background. Metabolic alterations as hyperglycemia and inflammation induce myocardial molecular events enhancing oxidative stress and mitochondrial dysfunction. Those alterations are responsible for a progressive loss of cardiomyocytes, cardiac stem cells, and consequent cardiovascular complications. Currently, there are no effective pharmacological measures to protect the heart from these metabolic modifications, and the development of new therapeutic approaches, focused on improvement of the oxidative stress condition, is pivotal. The protective effects of levocarnitine (LC) in patients with ischemic heart disease are related to the attenuation of oxidative stress, but LC mechanisms have yet to be fully understood. Objective. The aim of this work was to investigate LC’s role in oxidative stress condition, on ROS production and mitochondrial detoxifying function in H9c2 rat cardiomyocytes during hyperglycemia. Methods. H9c2 cells in the hyperglycemic state (25 mmol/L glucose) were exposed to 0.5 or 5 mM LC for 48 and 72 h: LC effects on signaling pathways involved in oxidative stress condition were studied by Western blot and immunofluorescence analysis. To evaluate ROS production, H9c2 cells were exposed to H2O2 after LC pretreatment. Results. Our in vitro study indicates how LC supplementation might protect cardiomyocytes from oxidative stress-related damage, preventing ROS formation and activating antioxidant signaling pathways in hyperglycemic conditions. In particular, LC promotes STAT3 activation and significantly increases the expression of antioxidant protein SOD2. Hyperglycemic cardiac cells are characterized by impairment in mitochondrial dysfunction and the CaMKII signal: LC promotes CaMKII expression and activation and enhancement of AMPK protein synthesis. Our results suggest that LC might ameliorate metabolic aspects of hyperglycemic cardiac cells. Finally, LC doses herein used did not modify H9c2 growth rate and viability. Conclusions. Our novel study demonstrates that LC improves the microenvironment damaged by oxidative stress (induced by hyperglycemia), thus proposing this nutraceutical compound as an adjuvant in diabetic cardiac regenerative medicine.

The SOD-2 protein is the single active SOD enzyme in C. elegans

2020 ◽  
Author(s):  
Lourds M. Fernando ◽  
Silwat Adeel ◽  
Mohammed Abul Basar ◽  
Anna K. Allen ◽  
Atanu Duttaroy
Keyword(s):  
Oxidative Stress ◽  
Stress Condition ◽  
Activity Analysis ◽  
Sod Activity ◽  
Mutant Analysis ◽  
Oxidative Stress Condition ◽  
E Coli ◽  
C Elegans ◽  
Aerobic Organism

ABSTRACTThe nematode C. elegans has a contingent of five sod genes, one of the largest among aerobic organism. Earlier studies revealed each of the five sod genes is capable of making perfectly active SOD proteins in heterologous expressions systems therefore none appears to be a pseudogene. Yet deletion of the entire contingent of sod genes fails to impose any effect on the survival of C. elegans except these animals appear more sensitive to extraneously applied oxidative stress condition. We asked how many of the five sod genes are actually active in C. elegans through an in-gel SOD activity analysis. Here we provide evidence that out of the five genes only the mitochondrial SOD gene is active in C. elegans, albeit at a much lesser amount compared to D. melanogaster and E. coli. Mutant analysis further confirmed that among the mitochondrial forms, SOD-2 is the only naturally active SOD in C. elegans.

scholarly journals Tissue Mechanisms of Adult Zebrafish Ventricular ECG Patterns under Baseline and Oxidative Stress Condition

2020 ◽  
Vol 118 (3) ◽  
pp. 259a
Author(s):  
Yali Zhao ◽  
Nicholas James ◽  
Ashraf Beshay ◽  
Eileen Chang ◽  
Thao P. Nguyen
Keyword(s):  
Oxidative Stress ◽  
Stress Condition ◽  
Adult Zebrafish ◽  
Oxidative Stress Condition ◽  
And Oxidative Stress

scholarly journals Effect of 6 Weeks of Aerobic Training on the Oxidative Stress Condition of Male Wistar Rats Exposed To Particulate Matter

2021 ◽  
Author(s):  
Zahra Eshaghi ◽  
Abdolhamid Habibi ◽  
Seyed Reza Fatemi Tabatabaei ◽  
Gholamreza Goudarzi
Keyword(s):  
Oxidative Stress ◽  
Particulate Matter ◽  
Wistar Rats ◽  
Antioxidant Defense ◽  
Aerobic Training ◽  
Stress Condition ◽  
Infected Group ◽  
Oxidative Stress Condition ◽  
Significant Difference ◽  
Male Wistar Rats

Introduction: Scientific evidence suggests the role of environmental pollutants, especially particulate mater in oxidative stress. However, the regular aerobic training has an important role in enhancing antioxidant defense and resistance to oxidative stress. Therefore, the purpose of this study was to investigate the effect of 6 weeks of aerobic training on the oxidative stress condition of male Wistar rats exposed to particulate matter. Methods: In this experimental study, 32 male rats (245 ± 8.5 g) aged 10 weeks, were divided into 4 groups of 8 rats each. The rats received the infusion of the particulate matter for 6 weeks through the nose. The exercise program included 6 weeks of aerobic training with 50% of maximum exercise; at the end of 6 weeks of the program, 24 hours after the last exercise session, blood samples were taken. Descriptive one-way analysis of variance (ANOVA) was used in different groups. If analysis were significant, differences between groups were estimated using LSD test. Significance was defined as P≤0.05. Statistical analysis was performed using SPSS software version 16. Results: After 6 weeks of aerobic training, levels of Malondialdehyde in both groups: healthy exercise and particulate matter+exercise decreased significantly and in the infected group (P≤0.05), but did not show significant difference compared to the control group (P≥0.05). The level of Superoxide dismutase in the infected group showed a significant increase. There was no significant difference between the two Glutathione peroxidas and catalase enzymes in each group (P≥0.05). Conclusion: Based on the results, it can be concluded that aerobic training may be effective in reducing lipid peroxidation and enhancing antioxidant defense.

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