scholarly journals PURIFICATION AND BIOLOGICAL ACTIVITIES OF COMBINATION OF HEMI-SYNTHESIZED THIOSEMICARBAZONES OF MITRACARPUS SCABER ZUCC

2020 ◽  
Author(s):  
BAMBOLA Bouraima ◽  
HOUNGBEME Gouton Alban ◽  
FAGBOHOUN Louis ◽  
MEDEGAN Sèdami ◽  
GBAGUIDI Ahokanou Fernand
Keyword(s):  
Liquid Chromatography ◽  
Peer Review ◽  
Biological Activities ◽  
Ethanolic Extract ◽  
Medium Pressure ◽  
Crude Extracts ◽  
Medium Pressure Liquid Chromatography ◽  
Review Reports ◽  
Mitracarpus Scaber ◽  
E Mail

The harvested Mitracarpus Scaber plants are identified in the national herbarium and registered under the number AA. 6252/HLB. During this work, three crude extracts are prepared from three organic solvents, namely dichloromethane; ethanol and hydro ethanol in 50/50 v/v proportion. The respective alkaloid extracts obtained from the corresponding crude extracts served as substrates for the hemi synthesis of thiosemicarbazone totals from thiosemicarbazides. The three hemi-synthesis products obtained were tested on eight (08) strains of germs, namely E. coli ATCC 25922, S. aureus ATCC 25923, E. faecalis ATCC 22921, P. aeruginosa ATCC 27853, C. albicans ATCC 10231, S. typhi, K. pneumoniae and Dermatophilus 146. The ethanolic extract of thiosemicarbazones exhibited the best bioactive activity and was found to be the most selective. By a series of bio guided chromatographies: TLC thin layer chromatography; CPA atmospheric pressure chromatography and medium pressure liquid chromatography. MPLC (medium pressure liquid chromatography), separation on dextran gel: Sephadex® LH20). The use of available spectral data, cross-checked with that of the literature, made it possible to identify and purify three (03) molecules of thiosemicarbazones, making it possible to study the biological activity of their combination. Peer Review History: UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. Received file Average Peer review marks at initial stage: 5.5/10 Average Peer review marks at publication stage: 8.0/10 Reviewer(s) detail: Name: Prof. Dr. Ali Gamal Ahmed Al-kaf Affiliation: Sana'a university, Yemen E-mail: [email protected]   Name: Prof. Dr. Amani S. Awaad Affiliation: College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj. KSA E-mail: [email protected] Comments of reviewer(s): Similar Articles: ANTIFUNGAL, CYTOTOXIC AND PHYTOTOXICITY OF AERIAL PART OF RANUNCULUS MURICATUS ANTI-INFLAMMATORY AND ANTI-OXIDANT ACTIVITIES OF METHANOL EXTRACT OF BAPHIA NITIDA

scholarly journals GREEN, RAPID, SIMPLE, AND AN EFFECTIVE ONE-POT MULTICOMPONENT STRATEGY FOR SYNTHESIS OF NOVEL DIHYDROPYRANO[2,3-C]PYRAZOL-6-AMINES IN AQUEOUS MEDIUM

2020 ◽  
Author(s):  
Sadeq Hamood Saleh Azzam ◽  
Aisha Siddekha ◽  
MA Pasha
Keyword(s):  
Aqueous Medium ◽  
Peer Review ◽  
Aromatic Aldehydes ◽  
Spectroscopic Techniques ◽  
One Pot ◽  
Excellent Yield ◽  
Unique System ◽  
Review Reports ◽  
Mitracarpus Scaber ◽  
E Mail

Objectives:  A series of new dihydropyrano [2,3-c]pyrazol-6-amines in this investigation  study, are synthesized  by the strategy of one-pot four component reaction in an aqueous medium. Methods:  After getting optimum conditions the reaction of different substituted aromatic aldehydes, ethyl acetoacetate, hydrazine hydrate,  and phenylacetonitrile catalysed by Sodium hydrogen sulphate NaHSO4 (0.5 m mol) by refluxing in ethanol-water (1:1) (5 ml) and heated at 80 oC. The synthesized molecules product was filtered, washed with water to get nearly pure product in excellent yield. Results:  The pure products which were got in a short duration, with excellent yield (80 – 87%). Those new compounds were identified by using spectroscopic techniques FT-IR, 1H-NMR and 13C-NMR. Conclusion: In conclusion, the usefulness of this one-pot multicomponents protocol include easily available starting materials, facile, simple and environment-friendly procedure, and easier separation of products by filtration. Peer Review History: Received 5 March 2019; Revised 11 April; Accepted 9 May, Available online 15 May 2020 UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. Received file Average Peer review marks at initial stage: 5.0/10 Average Peer review marks at publication stage: 7.0/10 Reviewer(s) detail: Name: Prof Cyprian Ogbonna ONYEJI Affiliation: Obafemi Awolowo University, Ile-Ife, Nigeria E-mail: [email protected]   Name: Dr. Md. Shahidul Islam Affiliation: USTC, Chittagong, Bangladesh E-mail: [email protected] Comments of reviewer(s): Similar Articles: TOXICITY AND ANTITRYPANOSOMAN ACTIVITY OF HEMI SYNTHESIS PRODUCTS OBTAINED FROM BIOACTIVE COMPOUNDS OF MITRACARPUS SCABER HARVESTED SOUTH OF BENIN

scholarly journals TOXICITY AND ANTITRYPANOSOMAN ACTIVITY OF HEMI SYNTHESIS PRODUCTS OBTAINED FROM BIOACTIVE COMPOUNDS OF MITRACARPUS SCABER HARVESTED SOUTH OF BENIN

2019 ◽  
Author(s):  
BAMBOLA Bouraïma ◽  
HOUNGBEME Gouton Alba ◽  
GANDONOU Dossa Clément ◽  
GBAGUIDI Ahokannou Fernand ◽  
FAGBOHOUN Louis ◽  
...  
Keyword(s):  
Peer Review ◽  
Research Centre ◽  
Toxicity Tests ◽  
Antitrypanosomal Activity ◽  
Unique System ◽  
Review Reports ◽  
Alkaloid Extracts ◽  
Mitracarpus Scaber ◽  
Larval Toxicity ◽  
E Mail

Present work involve study of the toxicity and the antitrypanosomal activity of the hemi syntheses (total of thiosemicarbazones) extracted from the substrates (reagents of thiosemicarbazides) in 1N hydrochloric acid medium from Mitracarpus Scaber harvested in the region of Abomey-calavi. The ethanolic, dichloromethane and hydroethanol extracts (50:50 v/v) yielded the alkaloid extracts with yields of 4.44%, 2.48% and 5.08% respectively leading to three products of hemi-synthesis P1, P2 and P3 whose larval toxicities have respective values LC50: 78.1μg/mL, 95μg/mL, and 48.8 μg/mL whereas the toxicity tests of the alkaloid samples of ethanol, dichloromethane and hydroethanolic E1, E2 and E3 extracted from the evaluation of the same larval toxicity test gave values of 83.41μg/mL 102.51μg/mL and 52.91μg/mL respectively. These results were less toxic than those of semisynthetic products. Acute and sub-acute toxicity in non-pregnant NMRI female mice after oral gavage of P2 product has been shown to be non-toxic. The antitrypanosomal test was carried out according to the Alamar blue method, it revealed that P1 moderately inhibits trypanosome parasites (IC50=18.06 μg/mL) as well as P2 and P3 with a respective IC50 of 17.24 μg/mL and 20, 68 μg/mL while the alkaloid totals had lower antitrypanosomal activity than the hemi-synthesis products. Peer Review History: UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. Received file Average Peer review marks at initial stage: 6.0/10 Average Peer review marks at publication stage: 8.5/10 Reviewer(s) detail: Name: Dr. Gehan Fawzy Abdel Raoof Kandeel Affiliation: Pharmacognosy Department, National Research Centre, Dokki, 12622,  Giza, Egypt E-mail: [email protected]   Name: Dr. Dalia Kamal Zaffar Ali  Affiliation: Modern University for technology and information, Egypt E-mail: [email protected] Comments of reviewer(s):

One-step Separation and Purification of Four Phenolic Acids from Stenoloma chusanum (L.) Ching by Medium-pressure Liquid Chromatography and High-speed Counter-current Chromatography

2019 ◽  
Vol 9 (2) ◽  
pp. 138-143
Author(s):  
Tianyun Li ◽  
Xiling Dai ◽  
Yichen Li ◽  
Guozheng Huang ◽  
Jianguo Cao
Keyword(s):  
Natural Products ◽  
Liquid Chromatography ◽  
Phenolic Acids ◽  
High Speed ◽  
High Efficiency ◽  
Total Phenolic ◽  
Medium Pressure ◽  
Medium Pressure Liquid Chromatography ◽  
One Step ◽  
Counter Current

Background:Stenoloma chusanum (L.) Ching is a Chinese traditional medicinal fern with high total flavonoid and total phenolic content. Traditionally, phenolic compounds were separated by using column chromatography, which is relatively inefficient. </P><P> Objective: This study aims to use an efficient method to separate natural products from S. chusanum by Medium-Pressure Liquid Chromatography (MPLC) and High-Speed Counter-Current Chromatography (HSCCC).Methods:In the present research, firstly, a sample (2.5 g) from the dichloromethane extract of S. chusanum was separated by MPLC. Next, fraction P5 was purified by HSCCC with a two-phase solvent system composed of hexane-ethyl acetate-methanol-water (HEMWat) at a volume ratio of 2:4:1:4 (v/v/v/v). </P><P> Result: Four phenolic acids were obtained and their structures were identified by means of NMR and ESI-mass analysis. They were identified as: 1) protocatechuic acid (34 mg, purity 90.1%), 2) syringic acid (66 mg, purity 99.0%), 3) p-hydroxybenzoic acid (5 mg, purity 91.2%) and 4) vanillic acid (6 mg, purity 99.3%).Conclusion:The combination of MPLC and HSCCC is a high-efficiency separation method for natural products. This is the first report with regard to the separation of four phenolic acids in one step by MPLC and HSCCC from S. chusanum (L.) Ching.

scholarly journals AN OVERVIEW ON CURRENT REGULATION AND EVALUATION OF BIOCIDAL PRODUCTS

2019 ◽  
Author(s):  
Aslı ŞAHİNER ◽  
Evren ALGIN YAPAR
Keyword(s):  
Peer Review ◽  
Chemical Substance ◽  
Biological Action ◽  
Insect Repellents ◽  
Biocidal Products ◽  
Unique System ◽  
Natural Oil ◽  
Review Reports ◽  
E Mail ◽  
Biocidal Product

A biocidal product is a substance or mixture prepared to limit, destroy, neutralize or control the effects of a harmful microorganism, plants and animals. The active substance in a biocidal product can be a natural oil or extract, a chemical substance or a microorganism, virus or fungus. Biocides consist of four main groups: disinfectants, preservatives (wood, paint, etc.), pest control and other type of biocidal products. A biocidal substance can also be added to a product to make the product itself into a biocidal product. These products range from disinfectants, hand sanitizers, preservatives, insect repellents, to rodenticides and insecticides and are used to protect humans, animals, materials and articles by controlling the intended target organism by a chemical or biological action. To make sure the use of biocidal products do not have unacceptable risks for people, animals and the environment, they are regulated to control their marketing, sale and use. In the current study biocidal products have been overviewed in the scope of current European Union regulations, product types and conformity tests. Peer Review History: UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. Received file Average Peer review marks at initial stage: 5.0/10 Average Peer review marks at publication stage: 7.0/10 Reviewer(s) detail: Name: Dr. Barkat Ali Khan Affiliation: Kampala International University , Uganda E-mail: [email protected]   Name: Dr. Sally A. El-Zahaby Affiliation: Pharos University in Alexandria, Egypt E-mail: [email protected] Comments of reviewer(s):

scholarly journals FORMULATION AND EVALUATION OF PHARMACEUTICALY STABLE SERUM OF DUCHESNEA INDICA (ARBENAN)

2021 ◽  
Author(s):  
A. Mumtihanah Mursyid ◽  
Risda Waris
Keyword(s):  
Peer Review ◽  
Leaf Extract ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Initial Stage ◽  
Unique System ◽  
Duchesnea Indica ◽  
Leaf Powder ◽  
Review Reports ◽  
Stable Leaf

Objective: Arbenan (Duchesnea indica) plants contain saponins, flavonoids, and tannins which have antioxidant activity. The purpose of this research is to perform formulation and evaluation extract ethanol of Arbenan leaves in the form of serum which is pharmaceutically stable. Method: Arbenan leaf powder was macerated with ethanol solvent, and then left for 3-4 days while stirring repeatedly, and then filtering. Furthermore, the liquid ethanol extract that has been obtained is evaporated using a Rotary Vacum Evaporator was used to evaporate the extract. Prepared extract was used to evaluate various parameters like organoleptics, homogeneity, viscosity, and pH. Result: All formulations were having typical smell, light brown color and a little thick consistency. Formulations of leaf extract of Arabenan with four variations bases have shown to have good stability after stress condition. It can be seen from the evaluation result are organoleptics, homogeneity, viscosity, rheology, and pH. Conclusion: Study concludes that a stable leaf extract of Arabenan can be effectively formulated into a serum by the means of various bases.                                     Peer Review History: Received 8 January 2021; Revised 13 February; Accepted 4 March, Available online 15 March 2021 UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency.  Received file:                Reviewer's Comments: Average Peer review marks at initial stage: 5.0/10 Average Peer review marks at publication stage: 7.0/10 Reviewer(s) detail: Dr. Marwa A. A. Fayed,  University of Sadat City, Egypt, [email protected] Prof. Dr. Ali Gamal Ahmed Al-kaf,  Sana'a university, Yemen, [email protected] Ahmad Najib, Universitas Muslim Indonesia, Makassar, Indonesia, [email protected] Similar Articles: PHYTOCHEMICAL, ANTI-INFLAMMATORY, ANALGESIC, ANTIPYRETIC AND ACUTE TOXICITY OF PSIADIA PUNCTULATA GROWING IN YEMEN ANTIHYPERLIPIDEMIC EFFECT OF THE ETHANOLIC EXTRACT OF SCAEVOLA TACCADA (GAERTN) ROXB. LEAVES ETHNOBOTANY, PHYTOCHEMISTRY AND PHARMACOLOGY OF OCHNA SCHWEINFURTHIANA: A REVIEW

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