scholarly journals Influence of 2,4-D and BAP on callus growth and the subsequent regeneration of somatic embryos in long-term cultures of Pelargonium x domesticum cv. Madame Layal

2007 ◽  
Vol 10 (1) ◽  
pp. 0-0 ◽  
Author(s):  
Klaus-Thomas Haensch ◽  
Philip Schulz
Keyword(s):  
Somatic Embryos ◽  
Callus Growth ◽  
Pelargonium X Domesticum ◽  
Subsequent Regeneration

Somatic Embryogenesis and In vitro Plant Regeneration in Vigna trilobata (L.) Verd. - A Potential Pasture Legume

1970 ◽  
Vol 19 (1) ◽  
pp. 89-99
Author(s):  
K. Choudhary ◽  
M. Singh ◽  
M. S. Rathore ◽  
N. S. Shekhawat
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Somatic Embryos ◽  
Basal Medium ◽  
Long Term Study ◽  
Continuous Application ◽  
First Time ◽  
Pasture Legume

This long term study demonstrates for the first time that it is possible to propagate embryogenic Vigna trilobata and to subsequently initiate the differentiation of embryos into complete plantlets. Initiation of callus was possible on 2,4-D. Somatic embryos differentiated on modified MS basal nutrient medium with 1.0 mg/l  of 2,4-D and 0.5 mg/l  of Kn. Sustained cell division resulted in globular and heart shape stages of somatic embryos. Transfer of embryos on to a fresh modified MS basal medium with 0.5 mg/l of Kn and 0.5 mg/l of GA3 helped them to attain maturation and germination. However, the propagation of cells, as well as the differentiation of embryos, were inhibited by a continuous application of these growth regulators. For this reason, a long period on medium lacking these growth regulators was necessary before the differentiation of embryos occurred again. The consequences for improving the propagation of embryogenic cultures in Vigna species are discussed. Key words: Pasture  legume, Vigna trilobata, Globular, Heart shape, somatic embryogenesis D.O.I. 10.3329/ptcb.v19i1.4990 Plant Tissue Cult. & Biotech. 19(1): 89-99, 2009 (June)

Adaptation of Medicago rigidula to a cereal–pasture rotation in north-west Syria

1986 ◽  
Vol 107 (1) ◽  
pp. 179-186 ◽  
Author(s):  
A. M. Abd El-Moneim ◽  
P. S. Cocks
Keyword(s):  
North Africa ◽  
Farming System ◽  
Livestock Production ◽  
Grazing Management ◽  
North West ◽  
Selection Programme ◽  
Ley Farming ◽  
Medicago Rigidula ◽  
Subsequent Regeneration

SUMMARYThe ley-farming system (integrated cereal and livestock production in which cerealsrotate with self-regenerating pastures) is considered to be of great potential benefit to north Africa and west Asia. In the colder parts of this region (of which north Syria is typical) its application is limited by poor adaptation of commercial medic cultivars (mainly Medicago truncatula and M. littoralis). An extended selection programme hag identified M. rigidula as adapted to the soils and climate of the region but nothing is known of its adaptation to the ley-farming system itself.An experiment which included 23 selections of M. rigidula and one each of M. rotata and M. noeana was conducted over 3 years during which herbage production, seed yield, and the fate of seeds were observed during the 1st year when pastures were established, the 2nd year when wheat was sown, and the 3rd year when the pasture regenerated. Of the 400–800 kg seed/ha produced in the 1st year an average of 87% remained in the soil in spring of the 3rd year. The weight of seed regenerating in the 3rd year varied from 30 to nearly 170 kg/ha, and herbage production, especially in winter, depended heavily on the number of regenerating seedlings. The most productive regenerating pastures produced nearly 2 t/ha of dried herbage by 1 January, and more than 6 t/ha for the whole growing season.The results showed that there was sufficient residual and newly produced seed at the end of the 3rd year to be sure that subsequent regeneration would result in similar herbage yields in the 5th year, and that the pasture was assured of long-term persistence. The significance of this for livestock production is discussed, and it is concluded that the results should encourage further investigation of grazing management and socioeconomic factors seen as constraints to introducing the ley-farming system to north Syria.

scholarly journals Callus growth and ion composition in response to long-term NaCl-induced stress in two sugarcane (Saccharum sp.) cultivars

2017 ◽  
Vol 8 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Errabii Tomader ◽  
Bernard Gandonou Christophe ◽  
Bouhdid Samira ◽  
Abrini Jamal ◽  
Skali-Senhaji Nadia
Keyword(s):  
Callus Growth ◽  
Ion Composition ◽  
Induced Stress ◽  
Saccharum Sp

Somatic polyembryogenesis in embryogenic cell masses of Piceaabies (Norway spruce) and Pinustaeda (loblolly pine) after thawing from liquid nitrogen

1987 ◽  
Vol 17 (9) ◽  
pp. 1130-1134 ◽  
Author(s):  
P. K. Gupta ◽  
D. J. Durzan ◽  
B.J. Finkle
Keyword(s):  
Liquid Nitrogen ◽  
Loblolly Pine ◽  
Somatic Embryos ◽  
Cell Mass ◽  
Embryogenic Cell ◽  
Long Term Storage ◽  
Embryogenic Cell Lines ◽  
Improvement Programs ◽  
Term Storage

We describe a method for the possible cryopreservation of embryogenic callus of Piceaabies and Pinustaeda at −196 °C and the regeneration of somatic embryos from thawed cells of subcultured embryonal–suspensor masses. Piceaabies and Pinustaeda were frozen without cryoprotective agent, in the presence of dimethyl sulfoxide (10%), or in a mixture of polyethylene glycol, glucose, and dimethylsulfoxide (10, 8, and 10% w/v, respectively). Cell masses placed in plastic vials or aluminum envelopes were frozen at 1 °C/min to −30 °C and then immersed for 10 min in liquid nitrogen. Cells were thawed rapidly and placed on modified MS subculture medium. Six to seven somatic embryos per gram of fresh weight were regenerated from each piece of frozen cell mass as compared with 12–13 embryos per gram from unfrozen cells. Post-thaw cell growth was inhibited initially by up to 5 weeks. Inhibition was reversed after the third 10-day subculture. Results suggest that the long-term storage of embryogenic cell lines in liquid nitrogen may be feasible for tree improvement programs in circumstances where testing of progeny may take several years.

Successful long-term preservation of abscisic-acid-treated and desiccated carrot somatic embryos

1999 ◽  
Vol 18 (9) ◽  
pp. 749-753 ◽  
Author(s):  
H. Shiota ◽  
K. Tachibana ◽  
K. Watabe ◽  
H. Kamada
Keyword(s):  
Abscisic Acid ◽  
Somatic Embryos ◽  
Long Term Preservation

scholarly journals The Fate of Integrated Ri T-DNA rol Genes during Regeneration via Somatic Embryogenesis in Tylophora indica

2015 ◽  
Vol 2015 ◽  
pp. 1-16 ◽  
Author(s):  
Dipasree Roychowdhury ◽  
Binay Chaubey ◽  
Sumita Jha
Keyword(s):  
Somatic Embryogenesis ◽  
Embryogenic Callus ◽  
Somatic Embryos ◽  
Growth Morphology ◽  
Tylophora Indica ◽  
Transformed Plants ◽  
Indirect Somatic Embryogenesis ◽  
Spontaneous Regeneration ◽  
Callus Lines

The fate of integrated Ri T-DNA rol genes during regeneration via indirect somatic embryogenesis and stability of its effect on morphology and tylophorine content of Ri-transformed plants have been studied in Tylophora indica. Integration and expression of Ri T-DNA genes in transformed embryogenic callus lines derived from transformed root lines, 300 Ri-transformed somatic embryos, and 23 Ri-transformed plant lines were analysed. Fifty root lines studied showed integration and expression of four rol genes of TL-DNA. Spontaneous regeneration via indirect somatic embryogenesis was obtained from root lines that were TL+/TR−. Stable integration and expression of rol genes were observed in root lines, embryogenic callus lines, and the spontaneously induced somatic embryos. Nineteen out of the 23 Ri-transformed plant lines and their clones showed phenotypic and genetic stability over the period of 3 years. Four Ri-transformed plants were morphologically similar to nontransformed plants but showed variation with the integration and expression of the rolA gene and absence of other rol genes. Variant Ri-transformed plant line A428#1-V showed highest tylophorine content (2.93±0.03 mg gDW−1) among plant lines studied. The effects of T-DNA genes on growth, morphology, and tylophorine content of the Ri-transformed plants were stable in the long term culture.

scholarly journals Primary results of 2-dimensional electrophoresis for protein studies of Gentiana kurroo Royle somatic embryos derived from long-term embryogenic cell suspensions

2014 ◽  
Vol 83 (3) ◽  
pp. 247-250 ◽  
Author(s):  
Agnieszka Niedziela ◽  
Jan J. Rybczyński
Keyword(s):  
Cell Suspension ◽  
Gel Electrophoresis ◽  
Somatic Embryos ◽  
Expression Profiles ◽  
Polyacrylamide Gel Electrophoresis ◽  
Embryogenic Cell Suspension ◽  
Two Dimensional Gel Electrophoresis ◽  
Embryogenic Cell ◽  
Gentiana Kurroo

Two-dimensional gel electrophoresis was used to compare protein expression profiles between various stages of <em>Gentiana kurroo</em> Royle somatic embryos. Seven distinct stages (I–VII) were pointed out and measured from long-term embryogenic cell suspension. Isoelectric focusing was done in the pH intervals 3–10, and the second dimension was carried out with 13% SDS-polyacrylamide gel electrophoresis. Dependent on the stage from about 400 (stage IV) to more than 700 (stage II) protein spots were totally detected. The molecular weight of abundant proteins range from 12 to 70 kDa, however, majority of proteins were located between 20–49 kDa spots on the gels. The highest difference in the number of spots appeared in the case of globular embryo (stage I) and elongated cotyledonary stage (stage VII) with differences being about 130 spots. The relevance of embryogenic cell suspension choice for proteomic analysis as well as expediency of the increasing number of particular embryo stages is discussed.

scholarly journals Recurrent somatic embryogenesis in long-term cultures of Gentiana lutea L. as a source for synthetic seed production for medium-term preservation

2012 ◽  
Vol 64 (2) ◽  
pp. 809-817 ◽  
Author(s):  
Irina Holobiuc ◽  
R. Catana
Keyword(s):  
Somatic Embryogenesis ◽  
Seed Production ◽  
Somatic Embryos ◽  
Plant Material ◽  
Synthetic Seed ◽  
Sugar Alcohols ◽  
Medium Term ◽  
Gentiana Lutea

Our aim was to establish an efficient and reproducible system for producing synthetic seeds from recurrent somatic embryogenesis in long-term cultures of Gentiana lutea L. This species is a vulnerable medicinal plant, protected both at the national and international levels, and is included in different Red Lists and Books. In vitro culture, as an alternative to classical methods of preservation, allows for the cyclic multiplication of plant material and short-, medium- and long-term preservation of tissue collections. Biotechnological approaches allow for maintenance of the plant material in a confined space and protection against biotic and abiotic factors. Somatic embryogenesis (SE) is the most efficient way to regenerate plants, ensuring material for preservation and fundamental research. In our experiment, recurrent somatic embryogenesis was developed in long-term cultures in the presence of sugar alcohols (mannitol, sorbitol) and in the absence of growth factors. This process proceeded at a high rate, with adventive somatic embryos being generated in a continuous process, followed by maturation, germination and development into plants. To follow the somatic embryogenesis process, histological samples were made. We used these embryogenic cultures for synthetic seed production and medium-term conservation. The viability of somatic embryos after moderate osmotic stress treatment was tested using TTC. Our methodology relied on the induction of somatic embryogenesis in the presence of auxins in the first cycle of in vitro cultures, long-term high embryogenic culture maintenance in the presence of sugar alcohols and synthetic seed production.

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