scholarly journals ELECTRON MICROSCOPIC STUDIES ON MORPHOLOGICAL CHANGES OF CHLAMYDIA PSITTACI INDUCED BY A SMALL DOSE OF PENICILLIN

Uirusu ◽  
1973 ◽  
Vol 23 (3) ◽  
pp. 205-220
Author(s):  
Yoshitsugu YAMADA
Keyword(s):  
Small Dose ◽  
Morphological Changes ◽  
Chlamydia Psittaci ◽  
Electron Microscopic ◽  
Microscopic Studies

scholarly journals Differential distributions in tissues and efficacies of aztreonam and ceftazidime and in vivo bacterial morphological changes following treatment.

1997 ◽  
Vol 41 (2) ◽  
pp. 401-409 ◽  
Author(s):  
A Turcotte ◽  
M Simard ◽  
N J Morin ◽  
D Beauchamp ◽  
M G Bergeron
Keyword(s):  
Cell Walls ◽  
Morphological Changes ◽  
Enterobacter Cloacae ◽  
Control Group ◽  
Electron Microscopic ◽  
Penetration Ratio ◽  
Microscopic Studies ◽  
Bow Tie ◽  
Fibrin Clots

The differential tissue distributions of aztreonam and ceftazidime within fibrin clots infected with Pseudomonas aeruginosa, Enterobacter cloacae, and Serratia marcescens, their efficacies, and the in vivo bacterial morphological changes induced by these drugs were evaluated. Rabbits were given intravenously a single dose of 100 mg of either agents/kg of body weight. In the cores of the clots, the peak levels of both drugs were much lower than those observed in the peripheries and in serum. Aztreonam's half-lives within the peripheries and in the cores of the fibrin clots were up to six times higher than observed in serum, while ceftazidime's half-lives in clots were twice that observed in serum. This resulted in a much greater penetration ratio for aztreonam than for ceftazidime. Both drugs controlled the growth of P. aeruginosa in vivo, but E. cloacae and S. marcescens responded better to ceftazidime. Morphological changes were more abundant in the peripheries than in the cores of the clots. In the control group, P. aeruginosa's morphology in the cores was different than that in the peripheries of the clots. Against P. aeruginosa, aztreonam did induce morphological changes in the cores while ceftazidime did not. Electron microscopic studies revealed that morphological changes associated with aztreonam seemed different than those of ceftazidime. Along with elongation of bacteria, more bow tie and herniated bacteria were observed with aztreonam. Though both agents selectively affect PBP 3, as manifested by elongated bacteria, they induce in the peripheries of the clots thickening, breaks, and detachment in bacterial cell walls, alterations which are generally associated with antibiotics affecting PBP 1a and 1b.

An Electron Microscopic Study of the Human Gastric Epithelia with Special Reference to the Cardinal Morphological Changes of the Chief Cells Induced by Insulin Stimulus

1972 ◽  
Vol 30 ◽  
pp. 344-345
Author(s):  
Hiroshi Saito ◽  
Goro Asano ◽  
Kaoru Aihara ◽  
Katsunari Fukushi ◽  
Minoru Yoshida ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Morphological Changes ◽  
Regular Insulin ◽  
Endoscopic Biopsy ◽  
Granular Endoplasmic Reticulum ◽  
Ultrastructural Changes ◽  
Electron Microscopic ◽  
Chief Cells ◽  
Microscopic Studies ◽  
The One

This short communication is dealt with the ultrastructural changes of the chief cells in insulin stimulus in chronic gastritic condition. The bio gastro-endoscopic biopsy was obtained and pepsin activity of the gastric juice was measured in respective cases. Regular insulin of 0.15U/kg was administrated intra-muscularly and in pre-administration of insulin, 10 minutes, 20 minutes and 30 minutes after administration, biopsied specimens were subjected for electron microscopic studies.In the pre-treated chief cells, extensive development of the cysternal structures of the granular endoplasmic reticulum in basal aspect of the cytoplasm and spherical or oval shaped, light homogeneous zymogen granules in supranuclear region and especially apical aspect of the cytoplasm were featured. Moreover, other type of the chief cells as the one characterized by their fragmented and saccular dilated granular endoplasmic reticulum in basal aspect of the cytoplasm, also exist.

Electron Microscopic study of the polymyxin treated vibrio cholerae cells

1990 ◽  
Vol 48 (3) ◽  
pp. 642-643
Author(s):  
Tapas Kumar Mandal
Keyword(s):  
Vibrio Cholerae ◽  
Morphological Changes ◽  
Indian Subcontinent ◽  
Analytical Techniques ◽  
Polymyxin B ◽  
Electron Microscopic ◽  
Microscopic Studies ◽  
Bacterial Organism ◽  
Polypeptide Antibiotic ◽  
Dose Dependent

Infections by Vibrio Cholerae (a gram-negative bacterial organism) in the Indian subcontinent are frequently encoutered. The polypeptide antibiotic polymyxin-B (PB) is a clinically important antibiotic which is used to treat infections caused by a number of gr am-negative bacteria. It has been observed that PB causes disruption of u U1rastructure of outer membrane (OW) , as revealed by the electron microscopic studies for a number of bacteria, through the formation of blebs and crenations (finger-like structures) on the OM . In this context, it is relevant to look specifically at the morphological changes induced by PB on Vibrio cells. PB produces dose dependent changes in the surface topography of pathogenic Vibrio cholerae cells. The susceptibilities of various vibrio strains to PB are also studied through analytical techniques.

Electron Microscopic study of the parathyroid gland of the melatonin-treated hamster

1990 ◽  
Vol 48 (3) ◽  
pp. 328-329
Author(s):  
Shizuko Shoumura ◽  
Shoichi Emura ◽  
Tomo Yamahira ◽  
Tomoo Kawada ◽  
Hiroshi Oda ◽  
...  
Keyword(s):  
Body Weight ◽  
Pineal Gland ◽  
Parathyroid Gland ◽  
Morphological Changes ◽  
Ultrastructural Changes ◽  
Electron Microscopic ◽  
Golden Hamsters ◽  
Microscopic Studies ◽  
The Relationship ◽  
Average Body

Some studies have dealt with the relationship between the pineal gland and the parathyroid gland (PTG) Morphological changes suggest that the pineal gland may inhibit or stimulate the PTG. There are a few light microscopic studies on the effects of pineal extract on the PTG. However, there is no study on the effects of melatonin on the ultrastructure of the PTG. We investigated ultrastructural changes in the PTG of golden hamsters after administration of melatonin.Materials and methods. Three-month-old female golden hamsters with an average body weight of 130 g were divided into 5 groups of 5 animals each. One group was given 0.2.ml of distilled water as controls. The remaining groups were given 0.2 ml of melatonin solution at a dose of 0.8 mg/100 g body weight. The PTG of each group was removed under pentobarbital anesthesia at 1, 5, 24 and 48 hours after injection. The PTG was immersed in a mixture of 2.5% glutaraldehyde and 2% OsO4, dehydrated through acetone and embedded in Epon 812.

Light and electron microscopic studies of phrenic nerves after long-term electrical stimulation

1983 ◽  
Vol 58 (1) ◽  
pp. 84-91 ◽  
Author(s):  
Jung H. Kim ◽  
Elias E. Manuelidis ◽  
William W. L. Glenn ◽  
Yoshitaka Fukuda ◽  
Douglas S. Cole ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Morphological Changes ◽  
Electron Microscopic ◽  
Content Type ◽  
Bipolar Electrodes ◽  
Microscopic Studies ◽  
Phrenic Nerves ◽  
Focal Demyelination ◽  
Real Area

✓ Light and electron microscopic evaluations were carried out on canine phrenic nerves subjected to long-term electrical stimulation. A total of 34 stimulated and 19 control nerves were studied by light microscopy, and 10 stimulated and five control nerves were evaluated by electron microscopy. Except in a few cases in which a higher current was used, the current used for stimulation was between 1 and 2 mA. The pulse width was 150 µsec. The typical charge per pulse was 0.22 µC and charge density per pulse 1.125 µC/sq cm of real area. The total number of days of electrical stimulation in individual phrenic nerves ranged from 4 to 374. No morphological changes in the phrenic nerve that could be attributed to the electrical stimulation were observed by light or electron microscopic study. There were, however, two phrenic nerves cuffed with bipolar electrodes which showed focal demyelination at the electrode level, but these changes were caused by factors other than the electrical stimulation. The results of the studies have direct clinical implications to long-term stimulation of phrenic nerves.

Sign in / Sign up

Export Citation Format

Share Document